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Catalyzes the conversion of oxalurate (N-carbamoyl-2-oxoglycine) to oxamate and carbamoyl phosphate (PubMed:38888336)"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"oxamate + carbamoyl phosphate = N-carbamoyl-2-oxoglycine + phosphate","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:22984"},{"database":"ChEBI","id":"CHEBI:43474"},{"database":"ChEBI","id":"CHEBI:57824"},{"database":"ChEBI","id":"CHEBI:58228"},{"database":"ChEBI","id":"CHEBI:58363"}],"ecNumber":"2.1.3.5","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"38888336"}]},"physiologicalReactions":[{"directionType":"right-to-left","reactionCrossReference":{"database":"Rhea","id":"RHEA:22986"},"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"38888336"}]}]},{"commentType":"COFACTOR","cofactors":[{"name":"Mg(2+)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"38888336"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:18420"}}]},{"commentType":"BIOPHYSICOCHEMICAL PROPERTIES","kineticParameters":{"maximumVelocities":[{"velocity":27.0,"unit":"umol/min/mg","enzyme":"enzyme toward oxamate","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"38888336"}]},{"velocity":15.4,"unit":"umol/min/mg","enzyme":"enzyme toward carbamoyl phosphate","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"38888336"}]}],"michaelisConstants":[{"constant":36.9,"unit":"mM","substrate":"oxamate","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"38888336"}]},{"constant":1.3,"unit":"mM","substrate":"carbamoyl phosphate","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"38888336"}]}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"38888336"}],"value":"kcat is 58.5 sec(-1) with oxamate as substrate. kcat is 35.5 sec(-1) with carbamoyl phosphate as substrate."}]}},"phDependence":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"38888336"}],"value":"Optimum pH is 9.0 (PubMed:38888336). No enzyme activity is detected at pH 6.0 and 7.0 (PubMed:38888336). Activity increases in the pH range of 8.0-9.0 (PubMed:38888336)."}]},"temperatureDependence":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"38888336"}],"value":"Optimum temperature is 30 degrees Celsius."}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"38888336"}],"value":"Nitrogen metabolism; (S)-allantoin degradation"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"38888336"}],"value":"The OXTCase is composed of 3 subunits, AllF, AllG and AllH"}],"commentType":"SUBUNIT"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"38888336"}],"value":"The deletion mutant cannot produce oxamate during anaerobic allantoin degradation (PubMed:38888336). However, the levels of oxalate, produced via a glyoxylate shunt, significantly increase (PubMed:38888336)"}],"commentType":"DISRUPTION PHENOTYPE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"38888336"}],"value":"Oxalurate could not be used as a standard substance due to its unavailability, and the enzyme reaction was performed in the reverse direction: the synthesis of oxalurate from oxamate and carbamoyl phosphate"}],"commentType":"MISCELLANEOUS"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the AllH family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":271,"modifier":"EXACT"}},"description":"Oxamate carbamoyltransferase subunit AllH","featureId":"PRO_0000168646"}],"keywords":[{"id":"KW-0460","category":"Ligand","name":"Magnesium"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0808","category":"Molecular function","name":"Transferase"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-B1UJ6TNQ0SAS1","citationType":"submission","authors":["Chung E.","Allen E.","Araujo R.","Aparicio A.M.","Davis K.","Duncan M.","Federspiel N.","Hyman R.","Kalman S.","Komp C.","Kurdi O.","Lew H.","Lin D.","Namath A.","Oefner P.","Roberts D.","Schramm S.","Davis R.W."],"title":"Sequence of minutes 4-25 of Escherichia coli.","publicationDate":"JAN-1997","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / MG1655 / ATCC 47076","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"9278503","citationType":"journal article","authors":["Blattner F.R.","Plunkett G. 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