{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P0A8M6","secondaryAccessions":["O07992","O07995","P76367","Q8X8U3"],"uniProtkbId":"TMAR_ECOLI","entryAudit":{"firstPublicDate":"2005-06-07","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2005-06-07","entryVersion":125,"sequenceVersion":1},"annotationScore":5.0,"organism":{"scientificName":"Escherichia coli (strain K12)","taxonId":83333,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Enterobacterales","Enterobacteriaceae","Escherichia"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"36577380"}],"value":"Pole-localizer protein TmaR"}},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"PTS system regulator TmaR"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"33376208"}],"value":"Targeting of sugar metabolism-associated regulator"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"33376208"}],"value":"tmaR"},"synonyms":[{"value":"yeeX"}],"orderedLocusNames":[{"value":"b2007"},{"value":"JW1989"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"33376208"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"36577380"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37934665"}],"value":"Pole-localizer protein involved in the regulation of several cellular processes (PubMed:33376208, PubMed:36577380, PubMed:37934665). Regulated processes include sugar metabolism, cell motility and small RNA-mediated regulation (PubMed:33376208, PubMed:36577380, PubMed:37934665). TmaR controls the subcellular localization and the activity of PtsI, the general PTS system enzyme I (EI), by polar sequestration and release of PtsI (PubMed:33376208). Polar sequestration of PtsI restricts its activity, thus regulating sugar metabolism and enabling cell survival in mild acidic conditions (PubMed:33376208). TmaR does not affect localization of the phosphocarrier protein HPr (PubMed:33376208). It also plays a central role in cell motility (PubMed:37934665). It controls flagella production and, thus, cell motility and biofilm formation, by forming condensates that protect and stabilize flagella related transcripts at the poles (PubMed:37934665). In addition, TmaR affects small RNA-mediated regulation by recruiting the RNA-binding protein Hfq to the poles during certain stress conditions (PubMed:36577380). It plays a crucial role in Hfq condensation (PubMed:36577380)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"33376208"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"36577380"}],"value":"Regulated via phosphorylation (PubMed:33376208, PubMed:36577380). Phosphorylation of TmaR on Tyr-72 is required for the release of PtsI from the poles and for its activity (PubMed:33376208). Only phosphorylated TmaR can release PtsI from the poles, since the diffuse non-phosphorylated TmaR binds to PtsI quite irreversibly (PubMed:33376208)"}],"commentType":"ACTIVITY REGULATION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"33376208"}],"value":"Interacts directly with the PTS EI component PtsI (PubMed:33376208). Binding is reversible as long as TmaR can get phosphorylated (PubMed:33376208)"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"33376208"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37934665"}],"value":"Forms clusters that localize mainly near one pole of the cell (PubMed:33376208, PubMed:37934665). Forms condensates at the cell poles in an RNA-dependent manner (PubMed:37934665). Phosphorylation on Tyr-72 is required for the formation of polar clusters (PubMed:33376208). Polar localization does not require PtsI (PubMed:33376208)"}]},"subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"33376208"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37934665"}],"value":"Cytoplasm","id":"SL-0086"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"33376208"}],"value":"The amount of TmaR does not change much among the different growth phases"}],"commentType":"DEVELOPMENTAL STAGE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37934665"}],"value":"The two helices, which seemingly fold into a coiled-coil, and the C-terminal region are probably all necessary for TmaR condensation"}],"commentType":"DOMAIN"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"33376208"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"36577380"}],"value":"Phosphorylated on Tyr-72 (PubMed:33376208). Phosphorylation on Tyr-72 is important for activity and polar localization of PtsI (PubMed:33376208). Phosphorylation on Tyr-79 might be required for polar localization of Hfq (PubMed:36577380)"}],"commentType":"PTM"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"33376208"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"36577380"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37934665"}],"value":"Cells lacking this gene are hyperactive for PTS sugar consumption (PubMed:33376208). In the absence of TmaR, PtsI is diffuse, released from the poles, and, therefore, more active in sugar uptake (PubMed:33376208). The absence of the gene also leads to detrimental effects on the ability of cells to survive in mild acidic conditions, probably due to PtsI hyperactivity (PubMed:33376208). Absence of the gene severely impairs the capacity of E.coli to produce flagella (PubMed:37934665). The mutant shows a reduced swimming ability, is completely impaired in swarming on semi-solid agar and has a dramatically reduced ability to form biofilms (PubMed:37934665). It also abolishes polar localization of Hfq in late stationary phase cells and Hfq condensation (PubMed:36577380)"}],"commentType":"DISRUPTION PHENOTYPE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37934665"}],"value":"Overexpression of TmaR leads to the formation of chained cells, suggesting that the cells cannot complete division (PubMed:37934665). Overexpressed TmaR forms filaments (PubMed:37934665)"}],"commentType":"MISCELLANEOUS"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00683"},{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the pole-localizer TmaR family"}],"commentType":"SIMILARITY"},{"commentType":"SEQUENCE CAUTION","sequenceCautionType":"Erroneous initiation","sequence":"BAA15828.1","note":"Extended N-terminus.","evidences":[{"evidenceCode":"ECO:0000305"}]}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":109,"modifier":"EXACT"}},"description":"Pole-localizer protein TmaR","featureId":"PRO_0000072758"},{"type":"Coiled coil","location":{"start":{"value":14,"modifier":"EXACT"},"end":{"value":41,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00683"}]},{"type":"Modified residue","location":{"start":{"value":72,"modifier":"EXACT"},"end":{"value":72,"modifier":"EXACT"}},"description":"Phosphotyrosine","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"33376208"}]},{"type":"Modified residue","location":{"start":{"value":79,"modifier":"EXACT"},"end":{"value":79,"modifier":"EXACT"}},"description":"Phosphotyrosine","evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"36577380"}]},{"type":"Mutagenesis","location":{"start":{"value":15,"modifier":"EXACT"},"end":{"value":15,"modifier":"EXACT"}},"description":"Fails to form clusters and presents a diffused pattern throughout the cytoplasm.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37934665"}],"alternativeSequence":{"originalSequence":"V","alternativeSequences":["L"]}},{"type":"Mutagenesis","location":{"start":{"value":20,"modifier":"EXACT"},"end":{"value":20,"modifier":"EXACT"}},"description":"Fails to form clusters and presents a diffused pattern throughout the cytoplasm. Shows a reduced swimming ability, is completely impaired in swarming on semi-solid agar and has a dramatically reduced ability to form biofilms.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37934665"}],"alternativeSequence":{"originalSequence":"R","alternativeSequences":["P"]}},{"type":"Mutagenesis","location":{"start":{"value":26,"modifier":"EXACT"},"end":{"value":26,"modifier":"EXACT"}},"description":"Fails to form clusters and presents a diffused pattern throughout the cytoplasm.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37934665"}],"alternativeSequence":{"originalSequence":"R","alternativeSequences":["H"]}},{"type":"Mutagenesis","location":{"start":{"value":45,"modifier":"EXACT"},"end":{"value":45,"modifier":"EXACT"}},"description":"Fails to form clusters and presents a diffused pattern throughout the cytoplasm.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37934665"}],"alternativeSequence":{}},{"type":"Mutagenesis","location":{"start":{"value":46,"modifier":"EXACT"},"end":{"value":46,"modifier":"EXACT"}},"description":"Decreases TmaR clustering.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37934665"}],"alternativeSequence":{"originalSequence":"D","alternativeSequences":["K"]}},{"type":"Mutagenesis","location":{"start":{"value":48,"modifier":"EXACT"},"end":{"value":48,"modifier":"EXACT"}},"description":"Fails to form clusters and presents a diffused pattern throughout the cytoplasm. Shows a reduced swimming ability, is completely impaired in swarming on semi-solid agar and has a dramatically reduced ability to form biofilms.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37934665"}],"alternativeSequence":{"originalSequence":"L","alternativeSequences":["P"]}},{"type":"Mutagenesis","location":{"start":{"value":51,"modifier":"EXACT"},"end":{"value":51,"modifier":"EXACT"}},"description":"Localizes to the cell pole.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"33376208"}],"alternativeSequence":{"originalSequence":"Y","alternativeSequences":["F"]}},{"type":"Mutagenesis","location":{"start":{"value":59,"modifier":"EXACT"},"end":{"value":59,"modifier":"EXACT"}},"description":"Forms clusters that do not localize to the poles. Shows a reduced swimming ability, is completely impaired in swarming on semi-solid agar and has a dramatically reduced ability to form biofilms.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37934665"}],"alternativeSequence":{"originalSequence":"E","alternativeSequences":["K"]}},{"type":"Mutagenesis","location":{"start":{"value":72,"modifier":"EXACT"},"end":{"value":72,"modifier":"EXACT"}},"description":"Does not localize to the cell poles.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"33376208"}],"alternativeSequence":{"originalSequence":"Y","alternativeSequences":["D","E"]}},{"type":"Mutagenesis","location":{"start":{"value":72,"modifier":"EXACT"},"end":{"value":72,"modifier":"EXACT"}},"description":"Does not localize to the cell poles and is completely spread within the cytoplasm. Has no effect on survival in acidic conditions. Does not form foci. When overexpressed, forms polar aggregates and does not form chained cells.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"33376208"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37934665"}],"alternativeSequence":{"originalSequence":"Y","alternativeSequences":["F"]}},{"type":"Mutagenesis","location":{"start":{"value":73,"modifier":"EXACT"},"end":{"value":73,"modifier":"EXACT"}},"description":"Fails to form clusters and presents a diffused pattern throughout the cytoplasm. Shows a reduced swimming ability, is completely impaired in swarming on semi-solid agar and has a dramatically reduced ability to form biofilms.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37934665"}],"alternativeSequence":{"originalSequence":"E","alternativeSequences":["R"]}},{"type":"Mutagenesis","location":{"start":{"value":79,"modifier":"EXACT"},"end":{"value":79,"modifier":"EXACT"}},"description":"Localizes to the cell pole. Has no effect on survival in acidic conditions. Abolishes Hfq polar accumulation.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"33376208"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"36577380"}],"alternativeSequence":{"originalSequence":"Y","alternativeSequences":["F"]}},{"type":"Mutagenesis","location":{"start":{"value":91,"modifier":"EXACT"},"end":{"value":91,"modifier":"EXACT"}},"description":"Fails to form clusters and presents a diffused pattern throughout the cytoplasm. Shows a reduced swimming ability, is completely impaired in swarming on semi-solid agar and has a dramatically reduced ability to form biofilms.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37934665"}],"alternativeSequence":{"originalSequence":"R","alternativeSequences":["H"]}},{"type":"Mutagenesis","location":{"start":{"value":91,"modifier":"EXACT"},"end":{"value":91,"modifier":"EXACT"}},"description":"Fails to form clusters and presents a diffused pattern throughout the cytoplasm.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37934665"}],"alternativeSequence":{"originalSequence":"R","alternativeSequences":["S"]}},{"type":"Mutagenesis","location":{"start":{"value":95,"modifier":"EXACT"},"end":{"value":95,"modifier":"EXACT"}},"description":"Does not affect cluster formation.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37934665"}],"alternativeSequence":{"originalSequence":"K","alternativeSequences":["E"]}},{"type":"Sequence conflict","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"in Ref. 4; AA sequence","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"M","alternativeSequences":["D"]}},{"type":"Sequence conflict","location":{"start":{"value":7,"modifier":"EXACT"},"end":{"value":7,"modifier":"EXACT"}},"description":"in Ref. 4; AA sequence","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"S","alternativeSequences":["D"]}}],"keywords":[{"id":"KW-0175","category":"Domain","name":"Coiled coil"},{"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"id":"KW-0903","category":"Technical term","name":"Direct protein sequencing"},{"id":"KW-0597","category":"PTM","name":"Phosphoprotein"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0346","category":"Biological process","name":"Stress response"}],"references":[{"referenceNumber":1,"citation":{"id":"9097040","citationType":"journal article","authors":["Itoh T.","Aiba H.","Baba T.","Fujita K.","Hayashi K.","Inada T.","Isono K.","Kasai H.","Kimura S.","Kitakawa M.","Kitagawa M.","Makino K.","Miki T.","Mizobuchi K.","Mori H.","Mori T.","Motomura K.","Nakade S.","Nakamura Y.","Nashimoto H.","Nishio Y.","Oshima T.","Saito N.","Sampei G.","Seki Y.","Sivasundaram S.","Tagami H.","Takeda J.","Takemoto K.","Wada C.","Yamamoto Y.","Horiuchi T."],"citationCrossReferences":[{"database":"PubMed","id":"9097040"},{"database":"DOI","id":"10.1093/dnares/3.6.379"}],"title":"A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map.","publicationDate":"1996","journal":"DNA Res.","firstPage":"379","lastPage":"392","volume":"3"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / W3110 / ATCC 27325 / DSM 5911","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"9278503","citationType":"journal article","authors":["Blattner F.R.","Plunkett G. III","Bloch C.A.","Perna N.T.","Burland V.","Riley M.","Collado-Vides J.","Glasner J.D.","Rode C.K.","Mayhew G.F.","Gregor J.","Davis N.W.","Kirkpatrick H.A.","Goeden M.A.","Rose D.J.","Mau B.","Shao Y."],"citationCrossReferences":[{"database":"PubMed","id":"9278503"},{"database":"DOI","id":"10.1126/science.277.5331.1453"}],"title":"The complete genome sequence of Escherichia coli K-12.","publicationDate":"1997","journal":"Science","firstPage":"1453","lastPage":"1462","volume":"277"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / MG1655 / ATCC 47076","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"16738553","citationType":"journal article","authors":["Hayashi K.","Morooka N.","Yamamoto Y.","Fujita K.","Isono K.","Choi S.","Ohtsubo E.","Baba T.","Wanner B.L.","Mori H.","Horiuchi T."],"citationCrossReferences":[{"database":"PubMed","id":"16738553"},{"database":"DOI","id":"10.1038/msb4100049"}],"title":"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.","publicationDate":"2006","journal":"Mol. Syst. Biol.","firstPage":"E1","lastPage":"E5","volume":"2"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / W3110 / ATCC 27325 / DSM 5911","type":"STRAIN"}]},{"referenceNumber":4,"citation":{"id":"9868784","citationType":"journal article","authors":["Wasinger V.C.","Humphery-Smith I."],"citationCrossReferences":[{"database":"PubMed","id":"9868784"},{"database":"DOI","id":"10.1111/j.1574-6968.1998.tb13343.x"}],"title":"Small genes/gene-products in Escherichia coli K-12.","publicationDate":"1998","journal":"FEMS Microbiol. Lett.","firstPage":"375","lastPage":"382","volume":"169"},"referencePositions":["PROTEIN SEQUENCE OF 1-10"],"referenceComments":[{"value":"K12","type":"STRAIN"}]},{"referenceNumber":5,"citation":{"id":"33376208","citationType":"journal article","authors":["Szoke T.","Albocher N.","Govindarajan S.","Nussbaum-Shochat A.","Amster-Choder O."],"citationCrossReferences":[{"database":"PubMed","id":"33376208"},{"database":"DOI","id":"10.1073/pnas.2016017118"}],"title":"Tyrosine phosphorylation-dependent localization of TmaR that controls activity of a major bacterial sugar regulator by polar sequestration.","publicationDate":"2021","journal":"Proc. Natl. Acad. Sci. U.S.A.","firstPage":"0","lastPage":"0","volume":"118"},"referencePositions":["FUNCTION","ACTIVITY REGULATION","INTERACTION WITH PTSI","SUBCELLULAR LOCATION","DEVELOPMENTAL STAGE","PHOSPHORYLATION AT TYR-72","DISRUPTION PHENOTYPE","MUTAGENESIS OF TYR-51; TYR-72 AND TYR-79"],"referenceComments":[{"value":"K12 / BW25113","type":"STRAIN"}]},{"referenceNumber":6,"citation":{"id":"36577380","citationType":"journal article","authors":["Goldberger O.","Szoke T.","Nussbaum-Shochat A.","Amster-Choder O."],"citationCrossReferences":[{"database":"PubMed","id":"36577380"},{"database":"DOI","id":"10.1016/j.celrep.2022.111881"}],"title":"Heterotypic phase separation of Hfq is linked to its roles as an RNA chaperone.","publicationDate":"2022","journal":"Cell Rep.","firstPage":"111881","lastPage":"111881","volume":"41"},"referencePositions":["FUNCTION","ACTIVITY REGULATION","PROBABLE PHOSPHORYLATION AT TYR-79","DISRUPTION PHENOTYPE","MUTAGENESIS OF TYR-79"],"referenceComments":[{"value":"K12 / MG1655 / ATCC 47076","type":"STRAIN"}]},{"referenceNumber":7,"citation":{"id":"37934665","citationType":"journal article","authors":["Szoke T.","Goldberger O.","Albocher-Kedem N.","Barsheshet M.","Dezorella N.","Nussbaum-Shochat A.","Wiener R.","Schuldiner M.","Amster-Choder O."],"citationCrossReferences":[{"database":"PubMed","id":"37934665"},{"database":"DOI","id":"10.1016/j.celrep.2023.113393"}],"title":"Regulation of major bacterial survival strategies by transcripts sequestration in a membraneless organelle.","publicationDate":"2023","journal":"Cell Rep.","firstPage":"113393","lastPage":"113393","volume":"42"},"referencePositions":["FUNCTION","SUBCELLULAR LOCATION","DOMAIN","DISRUPTION PHENOTYPE","OVEREXPRESSION","MUTAGENESIS OF VAL-15; ARG-20; ARG-26; LEU-45; ASP-46; LEU-48; GLU-59; TYR-72; GLU-73; ARG-91 AND LYS-95"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"U00096","properties":[{"key":"ProteinId","value":"AAC75068.2"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AP009048","properties":[{"key":"ProteinId","value":"BAA15828.1"},{"key":"Status","value":"ALT_INIT"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"F64965","properties":[{"key":"EntryName","value":"F64965"}]},{"database":"RefSeq","id":"NP_416511.4","properties":[{"key":"NucleotideSequenceId","value":"NC_000913.3"}]},{"database":"RefSeq","id":"WP_000450409.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_CP009789.1"}]},{"database":"AlphaFoldDB","id":"P0A8M6","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"P0A8M6","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"4261588","properties":[{"key":"Interactions","value":"22"}]},{"database":"BioGRID","id":"850888","properties":[{"key":"Interactions","value":"1"}]},{"database":"DIP","id":"DIP-47887N","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"P0A8M6","properties":[{"key":"Number of interactors","value":"237"}]},{"database":"IntAct","id":"P0A8M6","properties":[{"key":"Interactions","value":"5"}]},{"database":"STRING","id":"511145.b2007","properties":[{"key":"Description","value":"-"}]},{"database":"jPOST","id":"P0A8M6","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"511145-b2007","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblBacteria","id":"AAC75068","properties":[{"key":"ProteinId","value":"AAC75068"},{"key":"GeneId","value":"b2007"}]},{"database":"GeneID","id":"67172069","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"946541","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ecj:JW1989","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"eco:b2007","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ecoc:C3026_11325","properties":[{"key":"Description","value":"-"}]},{"database":"PATRIC","id":"fig|511145.12.peg.2083","properties":[{"key":"GeneDesignation","value":"-"}]},{"database":"EchoBASE","id":"EB3172","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"COG2926","properties":[{"key":"ToxonomicScope","value":"Bacteria"}]},{"database":"HOGENOM","id":"CLU_153146_0_0_6","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"P0A8M6","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"ENMRDDY","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"90485at2","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"P0A8M6","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"EcoCyc:G7087-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"PRO","id":"PR:P0A8M6","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000000625","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"GO","id":"GO:0051286","properties":[{"key":"GoTerm","value":"C:cell tip"},{"key":"GoEvidenceType","value":"IDA:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"33376208"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"EXP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"33376208"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37934665"}]},{"database":"GO","id":"GO:0005829","properties":[{"key":"GoTerm","value":"C:cytosol"},{"key":"GoEvidenceType","value":"IDA:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"18304323"}]},{"database":"GO","id":"GO:1903066","properties":[{"key":"GoTerm","value":"P:regulation of protein localization to cell tip"},{"key":"GoEvidenceType","value":"IMP:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"33376208"}]},{"database":"HAMAP","id":"MF_00683","properties":[{"key":"EntryName","value":"Pole_loc_TmaR"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR007458","properties":[{"key":"EntryName","value":"DUF496"}]},{"database":"InterPro","id":"IPR053375","properties":[{"key":"EntryName","value":"UPF0265"}]},{"database":"NCBIfam","id":"NF003844","properties":[{"key":"EntryName","value":"PRK05423.1"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"NF040881","properties":[{"key":"EntryName","value":"PTS_reg_TmaR"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR39591","properties":[{"key":"EntryName","value":"UPF0265 PROTEIN YEEX"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR39591:SF1","properties":[{"key":"EntryName","value":"UPF0265 PROTEIN YEEX"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF04363","properties":[{"key":"EntryName","value":"DUF496"},{"key":"MatchStatus","value":"1"}]},{"database":"PIRSF","id":"PIRSF028773","properties":[{"key":"EntryName","value":"UCP028773"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLLLDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGEMKNGEAK","length":109,"molWeight":12778,"crc64":"C66EA94BA415B46D","md5":"CDE4763FCEFE996B6C1CFF110F9AA96B"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"ACTIVITY REGULATION":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"DEVELOPMENTAL STAGE":1,"DOMAIN":1,"PTM":1,"DISRUPTION PHENOTYPE":1,"MISCELLANEOUS":1,"SIMILARITY":1,"SEQUENCE CAUTION":1},"countByFeatureType":{"Chain":1,"Coiled coil":1,"Modified residue":2,"Mutagenesis":15,"Sequence conflict":2},"uniParcId":"UPI000013AC20"}}