{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P0A8E7","secondaryAccessions":["P77482","Q2MC00"],"uniProtkbId":"YAJQ_ECOLI","entryAudit":{"firstPublicDate":"2005-06-07","lastAnnotationUpdateDate":"2026-01-28","lastSequenceUpdateDate":"2008-01-15","entryVersion":130,"sequenceVersion":2},"annotationScore":5.0,"organism":{"scientificName":"Escherichia coli (strain K12)","taxonId":83333,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Enterobacterales","Enterobacteriaceae","Escherichia"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Nucleotide-binding protein YajQ"}}},"genes":[{"geneName":{"value":"yajQ"},"orderedLocusNames":[{"value":"b0426"},{"value":"JW5058"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12381839"}],"value":"Nucleotide-binding protein (PubMed:12381839). Binds ATP, GTP and tRNA (PubMed:12381839)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12381839"}],"value":"Monomer"}],"commentType":"SUBUNIT"},{"commentType":"INTERACTION","interactions":[{"interactantOne":{"uniProtKBAccession":"P0A8E7","intActId":"EBI-370752"},"interactantTwo":{"uniProtKBAccession":"P0A988","geneName":"dnaN","intActId":"EBI-542385"},"numberOfExperiments":2,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"P0A8E7","intActId":"EBI-370752"},"interactantTwo":{"uniProtKBAccession":"P0A9I5","geneName":"napD","intActId":"EBI-554985"},"numberOfExperiments":4,"organismDiffer":false}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12381839"}],"value":"Composed of two domains, each centered on a beta-sheet, connected by two helical segments"}],"commentType":"DOMAIN"},{"commentType":"MASS SPECTROMETRY","method":"Electrospray","molWeight":18212.0,"molWeightError":0.0,"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12381839"}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00632"},{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the YajQ family"}],"commentType":"SIMILARITY"},{"commentType":"SEQUENCE CAUTION","sequenceCautionType":"Erroneous initiation","sequence":"AAB40182.1","note":"Extended N-terminus.","evidences":[{"evidenceCode":"ECO:0000305"}]}],"features":[{"type":"Initiator methionine","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"Removed","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12381839"}]},{"type":"Chain","location":{"start":{"value":2,"modifier":"EXACT"},"end":{"value":163,"modifier":"EXACT"}},"description":"Nucleotide-binding protein YajQ","featureId":"PRO_0000106180"}],"keywords":[{"id":"KW-0067","category":"Ligand","name":"ATP-binding"},{"id":"KW-0342","category":"Ligand","name":"GTP-binding"},{"id":"KW-0547","category":"Ligand","name":"Nucleotide-binding"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-B1UJ6TNQ0SAS1","citationType":"submission","authors":["Chung E.","Allen E.","Araujo R.","Aparicio A.M.","Davis K.","Duncan M.","Federspiel N.","Hyman R.","Kalman S.","Komp C.","Kurdi O.","Lew H.","Lin D.","Namath A.","Oefner P.","Roberts D.","Schramm S.","Davis R.W."],"title":"Sequence of minutes 4-25 of Escherichia coli.","publicationDate":"JAN-1997","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / MG1655 / ATCC 47076","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"9278503","citationType":"journal article","authors":["Blattner F.R.","Plunkett G. III","Bloch C.A.","Perna N.T.","Burland V.","Riley M.","Collado-Vides J.","Glasner J.D.","Rode C.K.","Mayhew G.F.","Gregor J.","Davis N.W.","Kirkpatrick H.A.","Goeden M.A.","Rose D.J.","Mau B.","Shao Y."],"citationCrossReferences":[{"database":"PubMed","id":"9278503"},{"database":"DOI","id":"10.1126/science.277.5331.1453"}],"title":"The complete genome sequence of Escherichia coli K-12.","publicationDate":"1997","journal":"Science","firstPage":"1453","lastPage":"1462","volume":"277"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / MG1655 / ATCC 47076","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"16738553","citationType":"journal article","authors":["Hayashi K.","Morooka N.","Yamamoto Y.","Fujita K.","Isono K.","Choi S.","Ohtsubo E.","Baba T.","Wanner B.L.","Mori H.","Horiuchi T."],"citationCrossReferences":[{"database":"PubMed","id":"16738553"},{"database":"DOI","id":"10.1038/msb4100049"}],"title":"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.","publicationDate":"2006","journal":"Mol. Syst. Biol.","firstPage":"E1","lastPage":"E5","volume":"2"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / W3110 / ATCC 27325 / DSM 5911","type":"STRAIN"}]},{"referenceNumber":4,"citation":{"id":"10493123","citationType":"journal article","authors":["Fountoulakis M.","Takacs M.-F.","Berndt P.","Langen H.","Takacs B."],"citationCrossReferences":[{"database":"PubMed","id":"10493123"},{"database":"DOI","id":"10.1002/(sici)1522-2683(19990801)20:11<2181::aid-elps2181>3.0.co;2-q"}],"title":"Enrichment of low abundance proteins of Escherichia coli by hydroxyapatite chromatography.","publicationDate":"1999","journal":"Electrophoresis","firstPage":"2181","lastPage":"2195","volume":"20"},"referencePositions":["IDENTIFICATION BY MASS SPECTROMETRY"],"referenceComments":[{"value":"B / BL21","type":"STRAIN"}]},{"referenceNumber":5,"citation":{"id":"12381839","citationType":"journal article","authors":["Saveanu C.","Miron S.","Borza T.","Craescu C.T.","Labesse G.","Gagyi C.","Popescu A.","Schaeffer F.","Namane A.","Laurent-Winter C.","Barzu O.","Gilles A.-M."],"citationCrossReferences":[{"database":"PubMed","id":"12381839"},{"database":"DOI","id":"10.1110/ps.0217502"}],"title":"Structural and nucleotide-binding properties of YajQ and YnaF, two Escherichia coli proteins of unknown function.","publicationDate":"2002","journal":"Protein Sci.","firstPage":"2551","lastPage":"2560","volume":"11"},"referencePositions":["FUNCTION","NUCLEOTIDE BINDING","SUBUNIT","DOMAIN","MASS SPECTROMETRY"],"referenceComments":[{"value":"K12","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"U82664","properties":[{"key":"ProteinId","value":"AAB40182.1"},{"key":"Status","value":"ALT_INIT"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"U00096","properties":[{"key":"ProteinId","value":"AAC73529.2"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AP009048","properties":[{"key":"ProteinId","value":"BAE76206.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"B64772","properties":[{"key":"EntryName","value":"B64772"}]},{"database":"RefSeq","id":"NP_414960.2","properties":[{"key":"NucleotideSequenceId","value":"NC_000913.3"}]},{"database":"RefSeq","id":"WP_001138904.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_CP009789.1"}]},{"database":"AlphaFoldDB","id":"P0A8E7","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"P0A8E7","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"4259513","properties":[{"key":"Interactions","value":"28"}]},{"database":"DIP","id":"DIP-31840N","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"P0A8E7","properties":[{"key":"Number of interactors","value":"498"}]},{"database":"IntAct","id":"P0A8E7","properties":[{"key":"Interactions","value":"6"}]},{"database":"STRING","id":"511145.b0426","properties":[{"key":"Description","value":"-"}]},{"database":"jPOST","id":"P0A8E7","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"511145-b0426","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblBacteria","id":"AAC73529","properties":[{"key":"ProteinId","value":"AAC73529"},{"key":"GeneId","value":"b0426"}]},{"database":"GeneID","id":"86945340","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"93777034","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"945063","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ecj:JW5058","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"eco:b0426","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ecoc:C3026_02080","properties":[{"key":"Description","value":"-"}]},{"database":"PATRIC","id":"fig|1411691.4.peg.1851","properties":[{"key":"GeneDesignation","value":"-"}]},{"database":"EchoBASE","id":"EB3379","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"COG1666","properties":[{"key":"ToxonomicScope","value":"Bacteria"}]},{"database":"HOGENOM","id":"CLU_099839_1_0_6","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"P0A8E7","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"DFKGVGA","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"9801447at2","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"P0A8E7","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"EcoCyc:G6240-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"PRO","id":"PR:P0A8E7","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000000625","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"GO","id":"GO:0005829","properties":[{"key":"GoTerm","value":"C:cytosol"},{"key":"GoEvidenceType","value":"IDA:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"15911532"},{"evidenceCode":"ECO:0000314","source":"PubMed","id":"18304323"}]},{"database":"GO","id":"GO:0005524","properties":[{"key":"GoTerm","value":"F:ATP binding"},{"key":"GoEvidenceType","value":"IDA:EcoliWiki"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"12381839"}]},{"database":"GO","id":"GO:0005525","properties":[{"key":"GoTerm","value":"F:GTP binding"},{"key":"GoEvidenceType","value":"IDA:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"12381839"}]},{"database":"GO","id":"GO:0000166","properties":[{"key":"GoTerm","value":"F:nucleotide binding"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0000049","properties":[{"key":"GoTerm","value":"F:tRNA binding"},{"key":"GoEvidenceType","value":"IDA:EcoliWiki"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"12381839"}]},{"database":"GO","id":"GO:0006974","properties":[{"key":"GoTerm","value":"P:DNA damage response"},{"key":"GoEvidenceType","value":"IMP:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"23308060"}]},{"database":"GO","id":"GO:0042542","properties":[{"key":"GoTerm","value":"P:response to hydrogen peroxide"},{"key":"GoEvidenceType","value":"IMP:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"34072091"}]},{"database":"CDD","id":"cd11740","properties":[{"key":"EntryName","value":"YajQ_like"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.30.70.860:FF:000001","properties":[{"key":"EntryName","value":"UPF0234 protein YajQ"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.30.70.990:FF:000001","properties":[{"key":"EntryName","value":"UPF0234 protein YajQ"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.70.860","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.70.990","properties":[{"key":"EntryName","value":"YajQ-like, domain 2"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_00632","properties":[{"key":"EntryName","value":"YajQ"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR035571","properties":[{"key":"EntryName","value":"UPF0234-like_C"}]},{"database":"InterPro","id":"IPR035570","properties":[{"key":"EntryName","value":"UPF0234_N"}]},{"database":"InterPro","id":"IPR036183","properties":[{"key":"EntryName","value":"YajQ-like_sf"}]},{"database":"InterPro","id":"IPR007551","properties":[{"key":"EntryName","value":"YajQ/Smlt4090-like"}]},{"database":"NCBIfam","id":"NF003819","properties":[{"key":"EntryName","value":"PRK05412.1"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR30476","properties":[{"key":"EntryName","value":"UPF0234 PROTEIN YAJQ"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR30476:SF0","properties":[{"key":"EntryName","value":"UPF0234 PROTEIN YAJQ"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF04461","properties":[{"key":"EntryName","value":"YajQ"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF89963","properties":[{"key":"EntryName","value":"YajQ-like"},{"key":"MatchStatus","value":"2"}]}],"sequence":{"value":"MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDASKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGIESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKNFRD","length":163,"molWeight":18344,"crc64":"DCBD1D4EB1630111","md5":"43B40EEC41574E304AEB711BFDC83B82"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBUNIT":1,"INTERACTION":2,"DOMAIN":1,"MASS SPECTROMETRY":1,"SIMILARITY":1,"SEQUENCE CAUTION":1},"countByFeatureType":{"Initiator methionine":1,"Chain":1},"uniParcId":"UPI000018F705"}}