{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P0A7E3","secondaryAccessions":["P00495","Q2M7V6"],"uniProtkbId":"PYRE_ECOLI","entryAudit":{"firstPublicDate":"1986-07-21","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2007-01-23","entryVersion":145,"sequenceVersion":2},"annotationScore":5.0,"organism":{"scientificName":"Escherichia coli (strain K12)","taxonId":83333,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Enterobacterales","Enterobacteriaceae","Escherichia"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01208"}],"value":"Orotate phosphoribosyltransferase"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01208"}],"value":"OPRT"},{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01208"}],"value":"OPRTase"}],"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01208"}],"value":"2.4.2.10"}]}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"6349999"}],"value":"pyrE"},"orderedLocusNames":[{"value":"b3642"},{"value":"JW3617"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01208"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"8620002"}],"value":"Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP)"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:10380"},{"database":"ChEBI","id":"CHEBI:30839"},{"database":"ChEBI","id":"CHEBI:33019"},{"database":"ChEBI","id":"CHEBI:57538"},{"database":"ChEBI","id":"CHEBI:58017"}],"ecNumber":"2.4.2.10","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01208"}]}},{"commentType":"COFACTOR","cofactors":[{"name":"Mg(2+)","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01208"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:18420"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"8620002"}],"value":"Inhibited by sulfate and phosphate ions"}],"commentType":"ACTIVITY REGULATION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01208"}],"value":"Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"6349999"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"8620002"}],"value":"Homodimer"}],"commentType":"SUBUNIT"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01208"}],"value":"Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily"}],"commentType":"SIMILARITY"}],"features":[{"type":"Initiator methionine","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"Removed"},{"type":"Chain","location":{"start":{"value":2,"modifier":"EXACT"},"end":{"value":213,"modifier":"EXACT"}},"description":"Orotate phosphoribosyltransferase","featureId":"PRO_0000110694"},{"type":"Binding site","location":{"start":{"value":26,"modifier":"EXACT"},"end":{"value":26,"modifier":"EXACT"}},"description":"in other chain","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58017"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01208"}],"ligand":{"name":"5-phospho-alpha-D-ribose 1-diphosphate","id":"ChEBI:CHEBI:58017","note":"ligand shared between dimeric partners"}},{"type":"Binding site","location":{"start":{"value":34,"modifier":"EXACT"},"end":{"value":35,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30839"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01208"}],"ligand":{"name":"orotate","id":"ChEBI:CHEBI:30839"}},{"type":"Binding site","location":{"start":{"value":72,"modifier":"EXACT"},"end":{"value":73,"modifier":"EXACT"}},"description":"in other chain","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58017"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01208"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"8620002"}],"ligand":{"name":"5-phospho-alpha-D-ribose 1-diphosphate","id":"ChEBI:CHEBI:58017","note":"ligand shared between dimeric partners"}},{"type":"Binding site","location":{"start":{"value":99,"modifier":"EXACT"},"end":{"value":99,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58017"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01208"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"8620002"}],"ligand":{"name":"5-phospho-alpha-D-ribose 1-diphosphate","id":"ChEBI:CHEBI:58017","note":"ligand shared between dimeric partners"}},{"type":"Binding site","location":{"start":{"value":100,"modifier":"EXACT"},"end":{"value":100,"modifier":"EXACT"}},"description":"in other chain","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58017"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01208"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"8620002"}],"ligand":{"name":"5-phospho-alpha-D-ribose 1-diphosphate","id":"ChEBI:CHEBI:58017","note":"ligand shared between dimeric partners"}},{"type":"Binding site","location":{"start":{"value":103,"modifier":"EXACT"},"end":{"value":103,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58017"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01208"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"8620002"}],"ligand":{"name":"5-phospho-alpha-D-ribose 1-diphosphate","id":"ChEBI:CHEBI:58017","note":"ligand shared between dimeric partners"}},{"type":"Binding site","location":{"start":{"value":105,"modifier":"EXACT"},"end":{"value":105,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58017"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01208"}],"ligand":{"name":"5-phospho-alpha-D-ribose 1-diphosphate","id":"ChEBI:CHEBI:58017","note":"ligand shared between dimeric partners"}},{"type":"Binding site","location":{"start":{"value":124,"modifier":"EXACT"},"end":{"value":132,"modifier":"EXACT"}},"description":"in other chain","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58017"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01208"}],"ligand":{"name":"5-phospho-alpha-D-ribose 1-diphosphate","id":"ChEBI:CHEBI:58017","note":"ligand shared between dimeric partners"}},{"type":"Binding site","location":{"start":{"value":128,"modifier":"EXACT"},"end":{"value":128,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30839"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01208"}],"ligand":{"name":"orotate","id":"ChEBI:CHEBI:30839"}},{"type":"Binding site","location":{"start":{"value":156,"modifier":"EXACT"},"end":{"value":156,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30839"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01208"}],"ligand":{"name":"orotate","id":"ChEBI:CHEBI:30839"}},{"type":"Sequence conflict","location":{"start":{"value":133,"modifier":"EXACT"},"end":{"value":133,"modifier":"EXACT"}},"description":"in Ref. 1 and 2","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{}},{"type":"Helix","location":{"start":{"value":3,"modifier":"EXACT"},"end":{"value":14,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6TAK"}]},{"type":"Beta strand","location":{"start":{"value":17,"modifier":"EXACT"},"end":{"value":24,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6TAK"}]},{"type":"Beta strand","location":{"start":{"value":30,"modifier":"EXACT"},"end":{"value":35,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6TAK"}]},{"type":"Helix","location":{"start":{"value":37,"modifier":"EXACT"},"end":{"value":39,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6TAK"}]},{"type":"Helix","location":{"start":{"value":43,"modifier":"EXACT"},"end":{"value":60,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6TAK"}]},{"type":"Beta strand","location":{"start":{"value":65,"modifier":"EXACT"},"end":{"value":69,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6TAK"}]},{"type":"Turn","location":{"start":{"value":71,"modifier":"EXACT"},"end":{"value":73,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6TAK"}]},{"type":"Helix","location":{"start":{"value":74,"modifier":"EXACT"},"end":{"value":89,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6TAK"}]},{"type":"Beta strand","location":{"start":{"value":94,"modifier":"EXACT"},"end":{"value":98,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6TAK"}]},{"type":"Beta strand","location":{"start":{"value":104,"modifier":"EXACT"},"end":{"value":107,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6TAK"}]},{"type":"Beta strand","location":{"start":{"value":111,"modifier":"EXACT"},"end":{"value":114,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6TAK"}]},{"type":"Beta strand","location":{"start":{"value":118,"modifier":"EXACT"},"end":{"value":123,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6TAK"}]},{"type":"Beta strand","location":{"start":{"value":128,"modifier":"EXACT"},"end":{"value":130,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6TAK"}]},{"type":"Helix","location":{"start":{"value":131,"modifier":"EXACT"},"end":{"value":142,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6TAK"}]},{"type":"Beta strand","location":{"start":{"value":146,"modifier":"EXACT"},"end":{"value":155,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6TAK"}]},{"type":"Beta strand","location":{"start":{"value":161,"modifier":"EXACT"},"end":{"value":165,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6TAK"}]},{"type":"Helix","location":{"start":{"value":166,"modifier":"EXACT"},"end":{"value":174,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6TAK"}]},{"type":"Beta strand","location":{"start":{"value":178,"modifier":"EXACT"},"end":{"value":183,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6TAK"}]},{"type":"Helix","location":{"start":{"value":184,"modifier":"EXACT"},"end":{"value":192,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6TAK"}]},{"type":"Helix","location":{"start":{"value":195,"modifier":"EXACT"},"end":{"value":197,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6TAK"}]},{"type":"Helix","location":{"start":{"value":198,"modifier":"EXACT"},"end":{"value":211,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6TAK"}]}],"keywords":[{"id":"KW-0002","category":"Technical term","name":"3D-structure"},{"id":"KW-0903","category":"Technical term","name":"Direct protein sequencing"},{"id":"KW-0328","category":"Molecular function","name":"Glycosyltransferase"},{"id":"KW-0460","category":"Ligand","name":"Magnesium"},{"id":"KW-0665","category":"Biological process","name":"Pyrimidine biosynthesis"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0808","category":"Molecular function","name":"Transferase"}],"references":[{"referenceNumber":1,"citation":{"id":"6349999","citationType":"journal article","authors":["Poulsen P.","Jensen K.F.","Valentin-Hansen P.","Carlsson P.","Lundberg L.G."],"citationCrossReferences":[{"database":"PubMed","id":"6349999"},{"database":"DOI","id":"10.1111/j.1432-1033.1983.tb07641.x"}],"title":"Nucleotide sequence of the Escherichia coli pyrE gene and of the DNA in front of the protein-coding region.","publicationDate":"1983","journal":"Eur. J. Biochem.","firstPage":"223","lastPage":"229","volume":"135"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]","PARTIAL PROTEIN SEQUENCE","SUBUNIT"],"referenceComments":[{"value":"K12","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"6207018","citationType":"journal article","authors":["Poulsen P.","Bonekamp F.","Jensen K.F."],"citationCrossReferences":[{"database":"PubMed","id":"6207018"},{"database":"DOI","id":"10.1002/j.1460-2075.1984.tb02046.x"}],"title":"Structure of the Escherichia coli pyrE operon and control of pyrE expression by a UTP modulated intercistronic attenuation.","publicationDate":"1984","journal":"EMBO J.","firstPage":"1783","lastPage":"1790","volume":"3"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"]},{"referenceNumber":3,"citation":{"id":"7686882","citationType":"journal article","authors":["Burland V.D.","Plunkett G. III","Daniels D.L.","Blattner F.R."],"citationCrossReferences":[{"database":"PubMed","id":"7686882"},{"database":"DOI","id":"10.1006/geno.1993.1230"}],"title":"DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication.","publicationDate":"1993","journal":"Genomics","firstPage":"551","lastPage":"561","volume":"16"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / MG1655 / ATCC 47076","type":"STRAIN"}]},{"referenceNumber":4,"citation":{"id":"9278503","citationType":"journal article","authors":["Blattner F.R.","Plunkett G. III","Bloch C.A.","Perna N.T.","Burland V.","Riley M.","Collado-Vides J.","Glasner J.D.","Rode C.K.","Mayhew G.F.","Gregor J.","Davis N.W.","Kirkpatrick H.A.","Goeden M.A.","Rose D.J.","Mau B.","Shao Y."],"citationCrossReferences":[{"database":"PubMed","id":"9278503"},{"database":"DOI","id":"10.1126/science.277.5331.1453"}],"title":"The complete genome sequence of Escherichia coli K-12.","publicationDate":"1997","journal":"Science","firstPage":"1453","lastPage":"1462","volume":"277"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / MG1655 / ATCC 47076","type":"STRAIN"}]},{"referenceNumber":5,"citation":{"id":"16738553","citationType":"journal article","authors":["Hayashi K.","Morooka N.","Yamamoto Y.","Fujita K.","Isono K.","Choi S.","Ohtsubo E.","Baba T.","Wanner B.L.","Mori H.","Horiuchi T."],"citationCrossReferences":[{"database":"PubMed","id":"16738553"},{"database":"DOI","id":"10.1038/msb4100049"}],"title":"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.","publicationDate":"2006","journal":"Mol. Syst. Biol.","firstPage":"E1","lastPage":"E5","volume":"2"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / W3110 / ATCC 27325 / DSM 5911","type":"STRAIN"}]},{"referenceNumber":6,"citation":{"id":"9298644","citationType":"journal article","authors":["VanBogelen R.A.","Abshire K.Z.","Moldover B.","Olson E.R.","Neidhardt F.C."],"citationCrossReferences":[{"database":"PubMed","id":"9298644"},{"database":"DOI","id":"10.1002/elps.1150180805"}],"title":"Escherichia coli proteome analysis using the gene-protein database.","publicationDate":"1997","journal":"Electrophoresis","firstPage":"1243","lastPage":"1251","volume":"18"},"referencePositions":["IDENTIFICATION BY 2D-GEL"]},{"referenceNumber":7,"citation":{"id":"8620002","citationType":"journal article","authors":["Henriksen A.","Aghajari N.","Jensen K.F.","Gajhede M."],"citationCrossReferences":[{"database":"PubMed","id":"8620002"},{"database":"DOI","id":"10.1021/bi952226y"}],"title":"A flexible loop at the dimer interface is a part of the active site of the adjacent monomer of Escherichia coli orotate phosphoribosyltransferase.","publicationDate":"1996","journal":"Biochemistry","firstPage":"3803","lastPage":"3809","volume":"35"},"referencePositions":["X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) IN COMPLEX WITH DIPHOSPHATE ANALOG","FUNCTION","ACTIVITY REGULATION","SUBUNIT"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"X00781","properties":[{"key":"ProteinId","value":"CAA25358.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"V01578","properties":[{"key":"ProteinId","value":"CAA24899.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"L10328","properties":[{"key":"ProteinId","value":"AAA61995.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"U00096","properties":[{"key":"ProteinId","value":"AAC76666.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AP009048","properties":[{"key":"ProteinId","value":"BAE77650.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"D65165","properties":[{"key":"EntryName","value":"XJEC"}]},{"database":"RefSeq","id":"NP_418099.1","properties":[{"key":"NucleotideSequenceId","value":"NC_000913.3"}]},{"database":"RefSeq","id":"WP_000806177.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_CP009789.1"}]},{"database":"PDB","id":"1ORO","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.40 A"},{"key":"Chains","value":"A/B=1-213"}]},{"database":"PDB","id":"6TAI","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.55 A"},{"key":"Chains","value":"AAA/BBB=1-213"}]},{"database":"PDB","id":"6TAJ","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.60 A"},{"key":"Chains","value":"AAA/BBB=1-213"}]},{"database":"PDB","id":"6TAK","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.25 A"},{"key":"Chains","value":"AAA/BBB=1-213"}]},{"database":"PDBsum","id":"1ORO","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"6TAI","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"6TAJ","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"6TAK","properties":[{"key":"Description","value":"-"}]},{"database":"AlphaFoldDB","id":"P0A7E3","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"P0A7E3","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"4259572","properties":[{"key":"Interactions","value":"16"}]},{"database":"FunCoup","id":"P0A7E3","properties":[{"key":"Number of interactors","value":"725"}]},{"database":"IntAct","id":"P0A7E3","properties":[{"key":"Interactions","value":"1"}]},{"database":"STRING","id":"511145.b3642","properties":[{"key":"Description","value":"-"}]},{"database":"jPOST","id":"P0A7E3","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"511145-b3642","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblBacteria","id":"AAC76666","properties":[{"key":"ProteinId","value":"AAC76666"},{"key":"GeneId","value":"b3642"}]},{"database":"GeneID","id":"75173836","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"75202211","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"948157","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ecj:JW3617","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"eco:b3642","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ecoc:C3026_19735","properties":[{"key":"Description","value":"-"}]},{"database":"PATRIC","id":"fig|1411691.4.peg.3064","properties":[{"key":"GeneDesignation","value":"-"}]},{"database":"EchoBASE","id":"EB0801","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"COG0461","properties":[{"key":"ToxonomicScope","value":"Bacteria"}]},{"database":"HOGENOM","id":"CLU_074878_0_1_6","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"P0A7E3","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"SPFFMNA","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"9779060at2","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"P0A7E3","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"EcoCyc:OROPRIBTRANS-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"MetaCyc:OROPRIBTRANS-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"BRENDA","id":"2.4.2.10","properties":[{"key":"OrganismId","value":"2026"}]},{"database":"UniPathway","id":"UPA00070","properties":[{"key":"RectionId","value":"UER00119"}]},{"database":"EvolutionaryTrace","id":"P0A7E3","properties":[{"key":"Description","value":"-"}]},{"database":"PRO","id":"PR:P0A7E3","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000000625","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"IDA:EcoliWiki"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"9222315"}]},{"database":"GO","id":"GO:0005829","properties":[{"key":"GoTerm","value":"C:cytosol"},{"key":"GoEvidenceType","value":"IDA:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"18304323"}]},{"database":"GO","id":"GO:0000287","properties":[{"key":"GoTerm","value":"F:magnesium ion binding"},{"key":"GoEvidenceType","value":"IDA:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"2419315"},{"evidenceCode":"ECO:0000314","source":"PubMed","id":"7050303"},{"evidenceCode":"ECO:0000314","source":"PubMed","id":"8620002"}]},{"database":"GO","id":"GO:0004588","properties":[{"key":"GoTerm","value":"F:orotate phosphoribosyltransferase activity"},{"key":"GoEvidenceType","value":"IDA:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"9222315"}]},{"database":"GO","id":"GO:0042803","properties":[{"key":"GoTerm","value":"F:protein homodimerization activity"},{"key":"GoEvidenceType","value":"IDA:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"2419315"}]},{"database":"GO","id":"GO:0006207","properties":[{"key":"GoTerm","value":"P:'de novo' pyrimidine nucleobase biosynthetic process"},{"key":"GoEvidenceType","value":"IMP:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"8501045"}]},{"database":"GO","id":"GO:0044205","properties":[{"key":"GoTerm","value":"P:'de novo' UMP biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0006221","properties":[{"key":"GoTerm","value":"P:pyrimidine nucleotide biosynthetic process"},{"key":"GoEvidenceType","value":"IMP:EcoliWiki"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"8501045"}]},{"database":"GO","id":"GO:0046132","properties":[{"key":"GoTerm","value":"P:pyrimidine ribonucleoside biosynthetic process"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"CDD","id":"cd06223","properties":[{"key":"EntryName","value":"PRTases_typeI"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.40.50.2020:FF:000008","properties":[{"key":"EntryName","value":"Orotate phosphoribosyltransferase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.2020","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_01208","properties":[{"key":"EntryName","value":"PyrE"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR023031","properties":[{"key":"EntryName","value":"OPRT"}]},{"database":"InterPro","id":"IPR004467","properties":[{"key":"EntryName","value":"Or_phspho_trans_dom"}]},{"database":"InterPro","id":"IPR029057","properties":[{"key":"EntryName","value":"PRTase-like"}]},{"database":"InterPro","id":"IPR000836","properties":[{"key":"EntryName","value":"PRTase_dom"}]},{"database":"NCBIfam","id":"TIGR00336","properties":[{"key":"EntryName","value":"pyrE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR46683","properties":[{"key":"EntryName","value":"OROTATE PHOSPHORIBOSYLTRANSFERASE 1-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR46683:SF1","properties":[{"key":"EntryName","value":"OROTATE PHOSPHORIBOSYLTRANSFERASE 1-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00156","properties":[{"key":"EntryName","value":"Pribosyltran"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF53271","properties":[{"key":"EntryName","value":"PRTase-like"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00103","properties":[{"key":"EntryName","value":"PUR_PYR_PR_TRANSFER"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGRDLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEAKDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERGRGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV","length":213,"molWeight":23567,"crc64":"F618A6D0A119B9D4","md5":"2C13089CCC8AC8947D75994BEF52960E"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"COFACTOR":1,"ACTIVITY REGULATION":1,"PATHWAY":1,"SUBUNIT":1,"SIMILARITY":1},"countByFeatureType":{"Initiator methionine":1,"Chain":1,"Binding site":10,"Sequence conflict":1,"Helix":9,"Beta strand":11,"Turn":1},"uniParcId":"UPI00001128D0"}}