{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P0A4Q0","secondaryAccessions":["Q54916"],"uniProtkbId":"PLSY_STRR6","entryAudit":{"firstPublicDate":"2005-03-15","lastAnnotationUpdateDate":"2026-06-10","lastSequenceUpdateDate":"2005-03-15","entryVersion":107,"sequenceVersion":1},"annotationScore":5.0,"organism":{"scientificName":"Streptococcus pneumoniae (strain ATCC BAA-255 / R6)","taxonId":171101,"lineage":["Bacteria","Bacillati","Bacillota","Bacilli","Lactobacillales","Streptococcaceae","Streptococcus"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Glycerol-3-phosphate acyltransferase"}},"alternativeNames":[{"fullName":{"value":"Acyl-PO4 G3P acyltransferase"}},{"fullName":{"value":"Acyl-phosphate--glycerol-3-phosphate acyltransferase"}},{"fullName":{"value":"G3P acyltransferase"},"shortNames":[{"value":"GPAT"}],"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16949372"}],"value":"2.3.1.275"}]},{"fullName":{"value":"Lysophosphatidic acid synthase"},"shortNames":[{"value":"LPA synthase"}]}]},"genes":[{"geneName":{"value":"plsY"},"orderedLocusNames":[{"value":"spr0755"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16949372"}],"value":"Catalyzes the transfer of an acyl group from acyl-phosphate (acyl-PO(4)) to glycerol-3-phosphate (G3P) to form lysophosphatidic acid (LPA). This enzyme utilizes acyl-phosphate as fatty acyl donor, but not acyl-CoA or acyl-ACP"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"an acyl phosphate + sn-glycerol 3-phosphate = a 1-acyl-sn-glycero-3-phosphate + phosphate","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:34075"},{"database":"ChEBI","id":"CHEBI:43474"},{"database":"ChEBI","id":"CHEBI:57597"},{"database":"ChEBI","id":"CHEBI:57970"},{"database":"ChEBI","id":"CHEBI:59918"}],"ecNumber":"2.3.1.275","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16949372"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"hexadecanoyl phosphate + sn-glycerol 3-phosphate = 1-hexadecanoyl-sn-glycero-3-phosphate + phosphate","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:54968"},{"database":"ChEBI","id":"CHEBI:43474"},{"database":"ChEBI","id":"CHEBI:57518"},{"database":"ChEBI","id":"CHEBI:57597"},{"database":"ChEBI","id":"CHEBI:138436"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16949372"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:54969"},"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16949372"}]}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16949372"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17308305"}],"value":"Inhibited by acyl-CoA and 1-acylglycerol-3-phosphate"}],"commentType":"ACTIVITY REGULATION"},{"commentType":"BIOPHYSICOCHEMICAL PROPERTIES","kineticParameters":{"michaelisConstants":[{"constant":100.0,"unit":"uM","substrate":"glycerol-3-phosphate (at 37 degrees Celsius and at pH 7.4)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17308305"}]},{"constant":30.0,"unit":"uM","substrate":"acyl-phosphate (at 37 degrees Celsius and at pH 7.4)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17308305"}]}]},"phDependence":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17308305"}],"value":"Optimum pH is 7.4."}]}},{"texts":[{"value":"Lipid metabolism; phospholipid metabolism"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"Probably interacts with PlsX"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"value":"Cell membrane","id":"SL-0039"},"topology":{"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the PlsY family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":213,"modifier":"EXACT"}},"description":"Glycerol-3-phosphate acyltransferase","featureId":"PRO_0000188465"},{"type":"Topological domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":3,"modifier":"EXACT"}},"description":"Extracellular","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":4,"modifier":"EXACT"},"end":{"value":22,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":23,"modifier":"EXACT"},"end":{"value":51,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":52,"modifier":"EXACT"},"end":{"value":74,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":75,"modifier":"EXACT"},"end":{"value":80,"modifier":"EXACT"}},"description":"Extracellular","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":81,"modifier":"EXACT"},"end":{"value":100,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":101,"modifier":"EXACT"},"end":{"value":142,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":143,"modifier":"EXACT"},"end":{"value":161,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":162,"modifier":"EXACT"},"end":{"value":162,"modifier":"EXACT"}},"description":"Extracellular","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":163,"modifier":"EXACT"},"end":{"value":184,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":185,"modifier":"EXACT"},"end":{"value":213,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Mutagenesis","location":{"start":{"value":36,"modifier":"EXACT"},"end":{"value":36,"modifier":"EXACT"}},"description":"Loss of acyltransferase activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17308305"}],"alternativeSequence":{"originalSequence":"S","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":43,"modifier":"EXACT"},"end":{"value":43,"modifier":"EXACT"}},"description":"5% of wild-type activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17308305"}],"alternativeSequence":{"originalSequence":"N","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":46,"modifier":"EXACT"},"end":{"value":46,"modifier":"EXACT"}},"description":"Loss of acyltransferase activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17308305"}],"alternativeSequence":{"originalSequence":"R","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":102,"modifier":"EXACT"},"end":{"value":102,"modifier":"EXACT"}},"description":"Defect in substrate binding.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17308305"}],"alternativeSequence":{"originalSequence":"G","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":103,"modifier":"EXACT"},"end":{"value":103,"modifier":"EXACT"}},"description":"Defect in substrate binding.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17308305"}],"alternativeSequence":{"originalSequence":"G","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":104,"modifier":"EXACT"},"end":{"value":104,"modifier":"EXACT"}},"description":"Loss of acyltransferase activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17308305"}],"alternativeSequence":{"originalSequence":"K","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":185,"modifier":"EXACT"},"end":{"value":185,"modifier":"EXACT"}},"description":"0.6% of wild-type activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17308305"}],"alternativeSequence":{"originalSequence":"H","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":188,"modifier":"EXACT"},"end":{"value":188,"modifier":"EXACT"}},"description":"Loss of acyltransferase activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17308305"}],"alternativeSequence":{"originalSequence":"N","alternativeSequences":["A"]}}],"keywords":[{"id":"KW-1003","category":"Cellular component","name":"Cell membrane"},{"id":"KW-0444","category":"Biological process","name":"Lipid biosynthesis"},{"id":"KW-0443","category":"Biological process","name":"Lipid metabolism"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-0594","category":"Biological process","name":"Phospholipid biosynthesis"},{"id":"KW-1208","category":"Biological process","name":"Phospholipid metabolism"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0808","category":"Molecular function","name":"Transferase"},{"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"id":"KW-1133","category":"Domain","name":"Transmembrane helix"}],"references":[{"referenceNumber":1,"citation":{"id":"11544234","citationType":"journal article","authors":["Hoskins J.","Alborn W.E. Jr.","Arnold J.","Blaszczak L.C.","Burgett S.","DeHoff B.S.","Estrem S.T.","Fritz L.","Fu D.-J.","Fuller W.","Geringer C.","Gilmour R.","Glass J.S.","Khoja H.","Kraft A.R.","Lagace R.E.","LeBlanc D.J.","Lee L.N.","Lefkowitz E.J.","Lu J.","Matsushima P.","McAhren S.M.","McHenney M.","McLeaster K.","Mundy C.W.","Nicas T.I.","Norris F.H.","O'Gara M.","Peery R.B.","Robertson G.T.","Rockey P.","Sun P.-M.","Winkler M.E.","Yang Y.","Young-Bellido M.","Zhao G.","Zook C.A.","Baltz R.H.","Jaskunas S.R.","Rosteck P.R. Jr.","Skatrud P.L.","Glass J.I."],"citationCrossReferences":[{"database":"PubMed","id":"11544234"},{"database":"DOI","id":"10.1128/jb.183.19.5709-5717.2001"}],"title":"Genome of the bacterium Streptococcus pneumoniae strain R6.","publicationDate":"2001","journal":"J. Bacteriol.","firstPage":"5709","lastPage":"5717","volume":"183"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"ATCC BAA-255 / R6","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"16949372","citationType":"journal article","authors":["Lu Y.-J.","Zhang Y.-M.","Grimes K.D.","Qi J.","Lee R.E.","Rock C.O."],"citationCrossReferences":[{"database":"PubMed","id":"16949372"},{"database":"DOI","id":"10.1016/j.molcel.2006.06.030"}],"title":"Acyl-phosphates initiate membrane phospholipid synthesis in Gram-positive pathogens.","publicationDate":"2006","journal":"Mol. Cell","firstPage":"765","lastPage":"772","volume":"23"},"referencePositions":["FUNCTION","CATALYTIC ACTIVITY","ACTIVITY REGULATION","NOMENCLATURE"]},{"referenceNumber":3,"citation":{"id":"17308305","citationType":"journal article","authors":["Lu Y.-J.","Zhang F.","Grimes K.D.","Lee R.E.","Rock C.O."],"citationCrossReferences":[{"database":"PubMed","id":"17308305"},{"database":"DOI","id":"10.1074/jbc.m700374200"}],"title":"Topology and active site of PlsY: the bacterial acylphosphate:glycerol-3-phosphate acyltransferase.","publicationDate":"2007","journal":"J. Biol. Chem.","firstPage":"11339","lastPage":"11346","volume":"282"},"referencePositions":["TOPOLOGY","MUTAGENESIS OF SER-36; ASN-43; ARG-46; GLY-102; GLY-103; LYS-104; HIS-185 AND ASN-188","ACTIVITY REGULATION","BIOPHYSICOCHEMICAL PROPERTIES"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AE007317","properties":[{"key":"ProteinId","value":"AAK99559.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"C97966","properties":[{"key":"EntryName","value":"C97966"}]},{"database":"RefSeq","id":"WP_000628789.1","properties":[{"key":"NucleotideSequenceId","value":"NC_003098.1"}]},{"database":"AlphaFoldDB","id":"P0A4Q0","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"P0A4Q0","properties":[{"key":"Description","value":"-"}]},{"database":"STRING","id":"171101.spr0755","properties":[{"key":"Description","value":"-"}]},{"database":"SwissLipids","id":"SLP:000001798","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"171101-spr0755","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"45653793","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"spr:spr0755","properties":[{"key":"Description","value":"-"}]},{"database":"PATRIC","id":"fig|171101.6.peg.835","properties":[{"key":"GeneDesignation","value":"-"}]},{"database":"eggNOG","id":"COG0344","properties":[{"key":"ToxonomicScope","value":"Bacteria"}]},{"database":"HOGENOM","id":"CLU_081254_4_0_9","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"MetaCyc:MONOMER-14087","properties":[{"key":"Description","value":"-"}]},{"database":"BRENDA","id":"2.3.1.275","properties":[{"key":"OrganismId","value":"1960"}]},{"database":"SABIO-RK","id":"P0A4Q0","properties":[{"key":"Description","value":"-"}]},{"database":"UniPathway","id":"UPA00085","properties":[{"key":"RectionId","value":"-"}]},{"database":"Proteomes","id":"UP000000586","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"GO","id":"GO:0005886","properties":[{"key":"GoTerm","value":"C:plasma membrane"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0043772","properties":[{"key":"GoTerm","value":"F:acyl-phosphate glycerol-3-phosphate acyltransferase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0008654","properties":[{"key":"GoTerm","value":"P:phospholipid biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"HAMAP","id":"MF_01043","properties":[{"key":"EntryName","value":"PlsY"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR003811","properties":[{"key":"EntryName","value":"G3P_acylTferase_PlsY"}]},{"database":"NCBIfam","id":"TIGR00023","properties":[{"key":"EntryName","value":"glycerol-3-phosphate 1-O-acyltransferase PlsY"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR30309:SF0","properties":[{"key":"EntryName","value":"GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR30309","properties":[{"key":"EntryName","value":"INNER MEMBRANE PROTEIN YGIH"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF02660","properties":[{"key":"EntryName","value":"G3P_acyltransf"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM01207","properties":[{"key":"EntryName","value":"G3P_acyltransf"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MITIVLLILAYLLGSIPSGLWIGQVFFQINLREHGSGNTGTTNTFRILGKKAGMATFVIDFFKGTLATLLPIIFHLQGVSPLIFGLLAVIGHTFPIFAGFKGGKAVATSAGVIFGFAPIFCLYLAIIFFGALYLGSMISLSSVTASIAAVIGVLLFPLFGFILSNYDSLFIAIILALASLIIIRHKDNIARIKNKTENLVPWGLNLTHQDPKK","length":213,"molWeight":22929,"crc64":"22CB089C17750818","md5":"C73DD836AB4993E6878A62C832DF33CB"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":2,"ACTIVITY REGULATION":1,"BIOPHYSICOCHEMICAL PROPERTIES":1,"PATHWAY":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Topological domain":6,"Transmembrane":5,"Mutagenesis":8},"uniParcId":"UPI00000515E9"}}