{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P09377","secondaryAccessions":["Q2M8K2"],"uniProtkbId":"RHAS_ECOLI","entryAudit":{"firstPublicDate":"1989-07-01","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"1989-07-01","entryVersion":202,"sequenceVersion":1},"annotationScore":5.0,"organism":{"scientificName":"Escherichia coli (strain K12)","taxonId":83333,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Enterobacterales","Enterobacteriaceae","Escherichia"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01534"}],"value":"HTH-type transcriptional activator RhaS"}},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01534"}],"value":"L-rhamnose operon regulatory protein RhaS"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01534"}],"value":"rhaS"},"synonyms":[{"value":"rhaC2"}],"orderedLocusNames":[{"value":"b3905"},{"value":"JW3876"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01534"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"8230210"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"8757746"}],"value":"Activates expression of the rhaBAD and rhaT operons"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01534"},{"evidenceCode":"ECO:0000305"}],"value":"Binds DNA as a dimer"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01534"}],"value":"Cytoplasm","id":"SL-0086"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"8230210"}],"value":"Induced by RhaR in response to L-rhamnose. Binding of the cAMP receptor protein (CRP) is required for full expression"}],"commentType":"INDUCTION"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":278,"modifier":"EXACT"}},"description":"HTH-type transcriptional activator RhaS","featureId":"PRO_0000194563"},{"type":"Domain","location":{"start":{"value":174,"modifier":"EXACT"},"end":{"value":272,"modifier":"EXACT"}},"description":"HTH araC/xylS-type","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01534"}]},{"type":"DNA binding","location":{"start":{"value":191,"modifier":"EXACT"},"end":{"value":212,"modifier":"EXACT"}},"description":"H-T-H motif","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01534"}]},{"type":"DNA binding","location":{"start":{"value":239,"modifier":"EXACT"},"end":{"value":262,"modifier":"EXACT"}},"description":"H-T-H motif","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01534"}]},{"type":"Site","location":{"start":{"value":241,"modifier":"EXACT"},"end":{"value":241,"modifier":"EXACT"}},"description":"Interaction with sigma-70","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01534"}]},{"type":"Site","location":{"start":{"value":250,"modifier":"EXACT"},"end":{"value":250,"modifier":"EXACT"}},"description":"Interaction with sigma-70","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01534"}]},{"type":"Mutagenesis","location":{"start":{"value":201,"modifier":"EXACT"},"end":{"value":201,"modifier":"EXACT"}},"description":"48% of wild-type activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10464186"}],"alternativeSequence":{"originalSequence":"L","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":202,"modifier":"EXACT"},"end":{"value":202,"modifier":"EXACT"}},"description":"4% of wild-type activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10464186"}],"alternativeSequence":{"originalSequence":"R","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":205,"modifier":"EXACT"},"end":{"value":205,"modifier":"EXACT"}},"description":"28% of wild-type activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10464186"}],"alternativeSequence":{"originalSequence":"H","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":206,"modifier":"EXACT"},"end":{"value":206,"modifier":"EXACT"}},"description":"0.1% of wild-type activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10464186"}],"alternativeSequence":{"originalSequence":"R","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":246,"modifier":"EXACT"},"end":{"value":246,"modifier":"EXACT"}},"description":"66% of wild-type activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10464186"}],"alternativeSequence":{"originalSequence":"C","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":247,"modifier":"EXACT"},"end":{"value":247,"modifier":"EXACT"}},"description":"6.2% of wild-type activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10464186"}],"alternativeSequence":{"originalSequence":"G","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":247,"modifier":"EXACT"},"end":{"value":247,"modifier":"EXACT"}},"description":"0.9% of wild-type activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10464186"}],"alternativeSequence":{"originalSequence":"G","alternativeSequences":["R"]}},{"type":"Mutagenesis","location":{"start":{"value":248,"modifier":"EXACT"},"end":{"value":248,"modifier":"EXACT"}},"description":"0.9% of wild-type activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10464186"}],"alternativeSequence":{"originalSequence":"F","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":248,"modifier":"EXACT"},"end":{"value":248,"modifier":"EXACT"}},"description":"3.3% of wild-type activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10464186"}],"alternativeSequence":{"originalSequence":"F","alternativeSequences":["V"]}},{"type":"Mutagenesis","location":{"start":{"value":249,"modifier":"EXACT"},"end":{"value":249,"modifier":"EXACT"}},"description":"93% of wild-type activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10464186"}],"alternativeSequence":{"originalSequence":"S","alternativeSequences":["G"]}},{"type":"Mutagenesis","location":{"start":{"value":250,"modifier":"EXACT"},"end":{"value":250,"modifier":"EXACT"}},"description":"8.3% of wild-type activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10464186"}],"alternativeSequence":{"originalSequence":"D","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":251,"modifier":"EXACT"},"end":{"value":251,"modifier":"EXACT"}},"description":"87% of wild-type activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10464186"}],"alternativeSequence":{"originalSequence":"S","alternativeSequences":["G"]}},{"type":"Mutagenesis","location":{"start":{"value":252,"modifier":"EXACT"},"end":{"value":252,"modifier":"EXACT"}},"description":"31% of wild-type activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10464186"}],"alternativeSequence":{"originalSequence":"N","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":252,"modifier":"EXACT"},"end":{"value":252,"modifier":"EXACT"}},"description":"0.4% of wild-type activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10464186"}],"alternativeSequence":{"originalSequence":"N","alternativeSequences":["H"]}},{"type":"Mutagenesis","location":{"start":{"value":252,"modifier":"EXACT"},"end":{"value":252,"modifier":"EXACT"}},"description":"0.3% of wild-type activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10464186"}],"alternativeSequence":{"originalSequence":"N","alternativeSequences":["I"]}},{"type":"Mutagenesis","location":{"start":{"value":252,"modifier":"EXACT"},"end":{"value":252,"modifier":"EXACT"}},"description":"37% of wild-type activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10464186"}],"alternativeSequence":{"originalSequence":"N","alternativeSequences":["S"]}},{"type":"Mutagenesis","location":{"start":{"value":253,"modifier":"EXACT"},"end":{"value":253,"modifier":"EXACT"}},"description":"48% of wild-type activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10464186"}],"alternativeSequence":{"originalSequence":"H","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":253,"modifier":"EXACT"},"end":{"value":253,"modifier":"EXACT"}},"description":"32% of wild-type activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10464186"}],"alternativeSequence":{"originalSequence":"H","alternativeSequences":["Q"]}},{"type":"Mutagenesis","location":{"start":{"value":254,"modifier":"EXACT"},"end":{"value":254,"modifier":"EXACT"}},"description":"1.2% of wild-type activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10464186"}],"alternativeSequence":{"originalSequence":"F","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":255,"modifier":"EXACT"},"end":{"value":255,"modifier":"EXACT"}},"description":"80% of wild-type activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10464186"}],"alternativeSequence":{"originalSequence":"S","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":256,"modifier":"EXACT"},"end":{"value":256,"modifier":"EXACT"}},"description":"61% of wild-type activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10464186"}],"alternativeSequence":{"originalSequence":"T","alternativeSequences":["A"]}}],"keywords":[{"id":"KW-0010","category":"Molecular function","name":"Activator"},{"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"id":"KW-0238","category":"Molecular function","name":"DNA-binding"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0677","category":"Domain","name":"Repeat"},{"id":"KW-0684","category":"Biological process","name":"Rhamnose metabolism"},{"id":"KW-0804","category":"Biological process","name":"Transcription"},{"id":"KW-0805","category":"Biological process","name":"Transcription regulation"}],"references":[{"referenceNumber":1,"citation":{"id":"3316663","citationType":"journal article","authors":["Tobin J.F.","Schleif R.F."],"citationCrossReferences":[{"database":"PubMed","id":"3316663"},{"database":"DOI","id":"10.1016/0022-2836(87)90405-0"}],"title":"Positive regulation of the Escherichia coli L-rhamnose operon is mediated by the products of tandemly repeated regulatory genes.","publicationDate":"1987","journal":"J. Mol. Biol.","firstPage":"789","lastPage":"799","volume":"196"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"]},{"referenceNumber":2,"citation":{"id":"8346018","citationType":"journal article","authors":["Plunkett G. III","Burland V.","Daniels D.L.","Blattner F.R."],"citationCrossReferences":[{"database":"PubMed","id":"8346018"},{"database":"DOI","id":"10.1093/nar/21.15.3391"}],"title":"Analysis of the Escherichia coli genome. III. DNA sequence of the region from 87.2 to 89.2 minutes.","publicationDate":"1993","journal":"Nucleic Acids Res.","firstPage":"3391","lastPage":"3398","volume":"21"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / MG1655 / ATCC 47076","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"9278503","citationType":"journal article","authors":["Blattner F.R.","Plunkett G. III","Bloch C.A.","Perna N.T.","Burland V.","Riley M.","Collado-Vides J.","Glasner J.D.","Rode C.K.","Mayhew G.F.","Gregor J.","Davis N.W.","Kirkpatrick H.A.","Goeden M.A.","Rose D.J.","Mau B.","Shao Y."],"citationCrossReferences":[{"database":"PubMed","id":"9278503"},{"database":"DOI","id":"10.1126/science.277.5331.1453"}],"title":"The complete genome sequence of Escherichia coli K-12.","publicationDate":"1997","journal":"Science","firstPage":"1453","lastPage":"1462","volume":"277"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / MG1655 / ATCC 47076","type":"STRAIN"}]},{"referenceNumber":4,"citation":{"id":"16738553","citationType":"journal article","authors":["Hayashi K.","Morooka N.","Yamamoto Y.","Fujita K.","Isono K.","Choi S.","Ohtsubo E.","Baba T.","Wanner B.L.","Mori H.","Horiuchi T."],"citationCrossReferences":[{"database":"PubMed","id":"16738553"},{"database":"DOI","id":"10.1038/msb4100049"}],"title":"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.","publicationDate":"2006","journal":"Mol. Syst. Biol.","firstPage":"E1","lastPage":"E5","volume":"2"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / W3110 / ATCC 27325 / DSM 5911","type":"STRAIN"}]},{"referenceNumber":5,"citation":{"id":"8230210","citationType":"journal article","authors":["Egan S.M.","Schleif R.F."],"citationCrossReferences":[{"database":"PubMed","id":"8230210"},{"database":"DOI","id":"10.1006/jmbi.1993.1565"}],"title":"A regulatory cascade in the induction of rhaBAD.","publicationDate":"1993","journal":"J. Mol. Biol.","firstPage":"87","lastPage":"98","volume":"234"},"referencePositions":["FUNCTION","INDUCTION"],"referenceComments":[{"value":"ECL116","type":"STRAIN"}]},{"referenceNumber":6,"citation":{"id":"8757746","citationType":"journal article","authors":["Via P.","Badia J.","Baldoma L.","Obradors N.","Aguilar J."],"citationCrossReferences":[{"database":"PubMed","id":"8757746"},{"database":"DOI","id":"10.1099/13500872-142-7-1833"}],"title":"Transcriptional regulation of the Escherichia coli rhaT gene.","publicationDate":"1996","journal":"Microbiology","firstPage":"1833","lastPage":"1840","volume":"142"},"referencePositions":["FUNCTION"]},{"referenceNumber":7,"citation":{"id":"10464186","citationType":"journal article","authors":["Bhende P.M.","Egan S.M."],"citationCrossReferences":[{"database":"PubMed","id":"10464186"},{"database":"DOI","id":"10.1128/jb.181.17.5185-5192.1999"}],"title":"Amino acid-DNA contacts by RhaS: an AraC family transcription activator.","publicationDate":"1999","journal":"J. Bacteriol.","firstPage":"5185","lastPage":"5192","volume":"181"},"referencePositions":["DNA-BINDING","MUTAGENESIS OF LEU-201; ARG-202; HIS-205; ARG-206; CYS-246; GLY-247; PHE-248; SER-249; ASP-250; SER-251; ASN-252; HIS-253; PHE-254; SER-255 AND THR-256"],"referenceComments":[{"value":"ECL116","type":"STRAIN"}]},{"referenceNumber":8,"citation":{"id":"10940041","citationType":"journal article","authors":["Bhende P.M.","Egan S.M."],"citationCrossReferences":[{"database":"PubMed","id":"10940041"},{"database":"DOI","id":"10.1128/jb.182.17.4959-4969.2000"}],"title":"Genetic evidence that transcription activation by RhaS involves specific amino acid contacts with sigma 70.","publicationDate":"2000","journal":"J. Bacteriol.","firstPage":"4959","lastPage":"4969","volume":"182"},"referencePositions":["INTERACTION WITH SIGMA-70"],"referenceComments":[{"value":"ECL116","type":"STRAIN"}]},{"referenceNumber":9,"citation":{"id":"11073923","citationType":"journal article","authors":["Holcroft C.C.","Egan S.M."],"citationCrossReferences":[{"database":"PubMed","id":"11073923"},{"database":"DOI","id":"10.1128/jb.182.23.6774-6782.2000"}],"title":"Interdependence of activation at rhaSR by cyclic AMP receptor protein, the RNA polymerase alpha subunit C-terminal domain, and rhaR.","publicationDate":"2000","journal":"J. Bacteriol.","firstPage":"6774","lastPage":"6782","volume":"182"},"referencePositions":["REGULATION BY CRP"]},{"referenceNumber":10,"citation":{"id":"15342598","citationType":"journal article","authors":["Wickstrum J.R.","Egan S.M."],"citationCrossReferences":[{"database":"PubMed","id":"15342598"},{"database":"DOI","id":"10.1128/jb.186.18.6277-6285.2004"}],"title":"Amino acid contacts between sigma 70 domain 4 and the transcription activators RhaS and RhaR.","publicationDate":"2004","journal":"J. Bacteriol.","firstPage":"6277","lastPage":"6285","volume":"186"},"referencePositions":["INTERACTION WITH SIGMA-70"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"X06058","properties":[{"key":"ProteinId","value":"CAA29452.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"L19201","properties":[{"key":"ProteinId","value":"AAB03038.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"U00096","properties":[{"key":"ProteinId","value":"AAC76887.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AP009048","properties":[{"key":"ProteinId","value":"BAE77404.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"S40849","properties":[{"key":"EntryName","value":"S40849"}]},{"database":"RefSeq","id":"NP_418341.1","properties":[{"key":"NucleotideSequenceId","value":"NC_000913.3"}]},{"database":"RefSeq","id":"WP_000217137.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_CP009789.1"}]},{"database":"AlphaFoldDB","id":"P09377","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"P09377","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"4260998","properties":[{"key":"Interactions","value":"123"}]},{"database":"BioGRID","id":"852694","properties":[{"key":"Interactions","value":"5"}]},{"database":"DIP","id":"DIP-10694N","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"P09377","properties":[{"key":"Number of interactors","value":"370"}]},{"database":"IntAct","id":"P09377","properties":[{"key":"Interactions","value":"24"}]},{"database":"STRING","id":"511145.b3905","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"511145-b3905","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblBacteria","id":"AAC76887","properties":[{"key":"ProteinId","value":"AAC76887"},{"key":"GeneId","value":"b3905"}]},{"database":"GeneID","id":"75204579","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"948397","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ecj:JW3876","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"eco:b3905","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ecoc:C3026_21115","properties":[{"key":"Description","value":"-"}]},{"database":"PATRIC","id":"fig|1411691.4.peg.2800","properties":[{"key":"GeneDesignation","value":"-"}]},{"database":"EchoBASE","id":"EB0836","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"COG4977","properties":[{"key":"ToxonomicScope","value":"Bacteria"}]},{"database":"HOGENOM","id":"CLU_000445_88_5_6","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"P09377","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"GHYPSHW","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"2547276at2","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"P09377","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"EcoCyc:PD00221","properties":[{"key":"Description","value":"-"}]},{"database":"PRO","id":"PR:P09377","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000000625","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"AbasyAtlas","id":"P09377","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0003700","properties":[{"key":"GoTerm","value":"F:DNA-binding transcription factor activity"},{"key":"GoEvidenceType","value":"IMP:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"15342598"}]},{"database":"GO","id":"GO:0000976","properties":[{"key":"GoTerm","value":"F:transcription cis-regulatory region binding"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0019318","properties":[{"key":"GoTerm","value":"P:hexose metabolic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0045893","properties":[{"key":"GoTerm","value":"P:positive regulation of DNA-templated transcription"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0006355","properties":[{"key":"GoTerm","value":"P:regulation of DNA-templated transcription"},{"key":"GoEvidenceType","value":"IMP:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"8230210"}]},{"database":"CDD","id":"cd06977","properties":[{"key":"EntryName","value":"cupin_RhaR_RhaS-like_N"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.10.10.60:FF:000181","properties":[{"key":"EntryName","value":"HTH-type transcriptional activator RhaS"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"2.60.120.10:FF:000041","properties":[{"key":"EntryName","value":"HTH-type transcriptional activator RhaS"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.10.60","properties":[{"key":"EntryName","value":"Homeodomain-like"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.60.120.10","properties":[{"key":"EntryName","value":"Jelly Rolls"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_01534","properties":[{"key":"EntryName","value":"HTH_type_RhaS"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR003313","properties":[{"key":"EntryName","value":"AraC-bd"}]},{"database":"InterPro","id":"IPR050204","properties":[{"key":"EntryName","value":"AraC_XylS_family_regulators"}]},{"database":"InterPro","id":"IPR009057","properties":[{"key":"EntryName","value":"Homeodomain-like_sf"}]},{"database":"InterPro","id":"IPR037923","properties":[{"key":"EntryName","value":"HTH-like"}]},{"database":"InterPro","id":"IPR018060","properties":[{"key":"EntryName","value":"HTH_AraC"}]},{"database":"InterPro","id":"IPR018062","properties":[{"key":"EntryName","value":"HTH_AraC-typ_CS"}]},{"database":"InterPro","id":"IPR047220","properties":[{"key":"EntryName","value":"RhaR_RhaS-like_N"}]},{"database":"InterPro","id":"IPR014710","properties":[{"key":"EntryName","value":"RmlC-like_jellyroll"}]},{"database":"InterPro","id":"IPR020449","properties":[{"key":"EntryName","value":"Tscrpt_reg_AraC-type_HTH"}]},{"database":"InterPro","id":"IPR023609","properties":[{"key":"EntryName","value":"Tscrpt_reg_HTH_RhaS"}]},{"database":"NCBIfam","id":"NF010028","properties":[{"key":"EntryName","value":"PRK13503.1"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR46796:SF13","properties":[{"key":"EntryName","value":"HTH-TYPE TRANSCRIPTIONAL ACTIVATOR RHAS"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR46796","properties":[{"key":"EntryName","value":"HTH-TYPE TRANSCRIPTIONAL ACTIVATOR RHAS-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF02311","properties":[{"key":"EntryName","value":"AraC_binding"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF12833","properties":[{"key":"EntryName","value":"HTH_18"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR00032","properties":[{"key":"EntryName","value":"HTHARAC"}]},{"database":"SMART","id":"SM00342","properties":[{"key":"EntryName","value":"HTH_ARAC"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF46689","properties":[{"key":"EntryName","value":"Homeodomain-like"},{"key":"MatchStatus","value":"2"}]},{"database":"SUPFAM","id":"SSF51215","properties":[{"key":"EntryName","value":"Regulatory protein AraC"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00041","properties":[{"key":"EntryName","value":"HTH_ARAC_FAMILY_1"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS01124","properties":[{"key":"EntryName","value":"HTH_ARAC_FAMILY_2"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHGTGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ","length":278,"molWeight":32315,"crc64":"64264514484C0D2A","md5":"3625995567CF62D5B6D1056AA156BFB8"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"INDUCTION":1},"countByFeatureType":{"Chain":1,"Domain":1,"DNA binding":2,"Site":2,"Mutagenesis":21},"uniParcId":"UPI0000133854"}}