{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P09029","secondaryAccessions":["Q2MBQ7"],"uniProtkbId":"PURK_ECOLI","entryAudit":{"firstPublicDate":"1988-11-01","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"1997-11-01","entryVersion":195,"sequenceVersion":2},"annotationScore":5.0,"organism":{"scientificName":"Escherichia coli (strain K12)","taxonId":83333,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Enterobacterales","Enterobacteriaceae","Escherichia"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01928"},{"evidenceCode":"ECO:0000305"}],"value":"N5-carboxyaminoimidazole ribonucleotide synthase"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01928"},{"evidenceCode":"ECO:0000305"}],"value":"N5-CAIR synthase"}],"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01928"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"8117684"}],"value":"6.3.4.18"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01928"},{"evidenceCode":"ECO:0000305"}],"value":"5-(carboxyamino)imidazole ribonucleotide synthetase"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01928"}],"value":"purK"},"orderedLocusNames":[{"evidences":[{"evidenceCode":"ECO:0000312","source":"EMBL","id":"AAC73624.1"}],"value":"b0522"},{"evidences":[{"evidenceCode":"ECO:0000312","source":"EMBL","id":"BAE76299.1"}],"value":"JW0511"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01928"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"8117684"}],"value":"Catalyzes the ATP-dependent conversion of 5-aminoimidazole ribonucleotide (AIR) and HCO(3)(-) to N5-carboxyaminoimidazole ribonucleotide (N5-CAIR)"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"5-amino-1-(5-phospho-beta-D-ribosyl)imidazole + hydrogencarbonate + ATP = 5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole + ADP + phosphate + 2 H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:19317"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:17544"},{"database":"ChEBI","id":"CHEBI:30616"},{"database":"ChEBI","id":"CHEBI:43474"},{"database":"ChEBI","id":"CHEBI:58730"},{"database":"ChEBI","id":"CHEBI:137981"},{"database":"ChEBI","id":"CHEBI:456216"}],"ecNumber":"6.3.4.18","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01928"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"8117684"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01928"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"8117684"}],"value":"Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (N5-CAIR route): step 1/2"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01928"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10569930"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"19053251"}],"value":"Homodimer"}],"commentType":"SUBUNIT"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01928"},{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the PurK/PurT family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"2464576"}],"value":"Was originally thought to be the ATPase subunit of phosphoribosylaminoimidazole carboxylase, with catalytic subunit PurE"}],"commentType":"CAUTION"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":355,"modifier":"EXACT"}},"description":"N5-carboxyaminoimidazole ribonucleotide synthase","featureId":"PRO_0000074997"},{"type":"Domain","location":{"start":{"value":84,"modifier":"EXACT"},"end":{"value":267,"modifier":"EXACT"}},"description":"ATP-grasp","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01928"}]},{"type":"Binding site","location":{"start":{"value":80,"modifier":"EXACT"},"end":{"value":80,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01928"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10569930"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"19053251"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}},{"type":"Binding site","location":{"start":{"value":120,"modifier":"EXACT"},"end":{"value":120,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01928"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10569930"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"19053251"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}},{"type":"Binding site","location":{"start":{"value":125,"modifier":"EXACT"},"end":{"value":131,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01928"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10569930"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"19053251"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}},{"type":"Binding site","location":{"start":{"value":153,"modifier":"EXACT"},"end":{"value":156,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01928"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10569930"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"19053251"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}},{"type":"Binding site","location":{"start":{"value":161,"modifier":"EXACT"},"end":{"value":161,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01928"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10569930"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"19053251"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}},{"type":"Binding site","location":{"start":{"value":184,"modifier":"EXACT"},"end":{"value":184,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01928"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10569930"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"19053251"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}},{"type":"Binding site","location":{"start":{"value":237,"modifier":"EXACT"},"end":{"value":238,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01928"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10569930"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"19053251"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}},{"type":"Sequence conflict","location":{"start":{"value":64,"modifier":"EXACT"},"end":{"value":65,"modifier":"EXACT"}},"description":"in Ref. 1; CAA31421","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"RH","alternativeSequences":["PD"]}},{"type":"Beta strand","location":{"start":{"value":3,"modifier":"EXACT"},"end":{"value":8,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3ETH"}]},{"type":"Helix","location":{"start":{"value":11,"modifier":"EXACT"},"end":{"value":20,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3ETH"}]},{"type":"Helix","location":{"start":{"value":21,"modifier":"EXACT"},"end":{"value":23,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3ETH"}]},{"type":"Beta strand","location":{"start":{"value":26,"modifier":"EXACT"},"end":{"value":30,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3ETH"}]},{"type":"Helix","location":{"start":{"value":36,"modifier":"EXACT"},"end":{"value":38,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3ETH"}]},{"type":"Helix","location":{"start":{"value":41,"modifier":"EXACT"},"end":{"value":43,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3ETJ"}]},{"type":"Beta strand","location":{"start":{"value":44,"modifier":"EXACT"},"end":{"value":49,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3ETH"}]},{"type":"Helix","location":{"start":{"value":57,"modifier":"EXACT"},"end":{"value":63,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3ETH"}]},{"type":"Turn","location":{"start":{"value":69,"modifier":"EXACT"},"end":{"value":72,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3ETH"}]},{"type":"Helix","location":{"start":{"value":73,"modifier":"EXACT"},"end":{"value":78,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3ETH"}]},{"type":"Helix","location":{"start":{"value":80,"modifier":"EXACT"},"end":{"value":89,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3ETH"}]},{"type":"Beta strand","location":{"start":{"value":97,"modifier":"EXACT"},"end":{"value":100,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3ETH"}]},{"type":"Helix","location":{"start":{"value":103,"modifier":"EXACT"},"end":{"value":105,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3ETH"}]},{"type":"Helix","location":{"start":{"value":106,"modifier":"EXACT"},"end":{"value":113,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3ETH"}]},{"type":"Beta strand","location":{"start":{"value":115,"modifier":"EXACT"},"end":{"value":124,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3ETH"}]},{"type":"Turn","location":{"start":{"value":127,"modifier":"EXACT"},"end":{"value":130,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3ETH"}]},{"type":"Beta strand","location":{"start":{"value":131,"modifier":"EXACT"},"end":{"value":135,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3ETH"}]},{"type":"Turn","location":{"start":{"value":136,"modifier":"EXACT"},"end":{"value":138,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1B6S"}]},{"type":"Helix","location":{"start":{"value":139,"modifier":"EXACT"},"end":{"value":141,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3ETH"}]},{"type":"Helix","location":{"start":{"value":144,"modifier":"EXACT"},"end":{"value":146,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3ETH"}]},{"type":"Turn","location":{"start":{"value":147,"modifier":"EXACT"},"end":{"value":149,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3ETH"}]},{"type":"Beta strand","location":{"start":{"value":150,"modifier":"EXACT"},"end":{"value":154,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3ETH"}]},{"type":"Beta strand","location":{"start":{"value":159,"modifier":"EXACT"},"end":{"value":168,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3ETH"}]},{"type":"Beta strand","location":{"start":{"value":174,"modifier":"EXACT"},"end":{"value":176,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3ETH"}]},{"type":"Helix","location":{"start":{"value":177,"modifier":"EXACT"},"end":{"value":179,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3ETH"}]},{"type":"Beta strand","location":{"start":{"value":180,"modifier":"EXACT"},"end":{"value":185,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3ETH"}]},{"type":"Beta strand","location":{"start":{"value":188,"modifier":"EXACT"},"end":{"value":194,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3ETH"}]},{"type":"Helix","location":{"start":{"value":200,"modifier":"EXACT"},"end":{"value":217,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3ETH"}]},{"type":"Beta strand","location":{"start":{"value":221,"modifier":"EXACT"},"end":{"value":230,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3ETH"}]},{"type":"Beta strand","location":{"start":{"value":233,"modifier":"EXACT"},"end":{"value":242,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3ETH"}]},{"type":"Helix","location":{"start":{"value":245,"modifier":"EXACT"},"end":{"value":247,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3ETH"}]},{"type":"Helix","location":{"start":{"value":250,"modifier":"EXACT"},"end":{"value":253,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3ETH"}]},{"type":"Beta strand","location":{"start":{"value":254,"modifier":"EXACT"},"end":{"value":256,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3ETH"}]},{"type":"Helix","location":{"start":{"value":258,"modifier":"EXACT"},"end":{"value":266,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3ETH"}]},{"type":"Helix","location":{"start":{"value":272,"modifier":"EXACT"},"end":{"value":275,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3ETH"}]},{"type":"Beta strand","location":{"start":{"value":280,"modifier":"EXACT"},"end":{"value":287,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3ETH"}]},{"type":"Helix","location":{"start":{"value":292,"modifier":"EXACT"},"end":{"value":296,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3ETH"}]},{"type":"Beta strand","location":{"start":{"value":301,"modifier":"EXACT"},"end":{"value":304,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3ETH"}]},{"type":"Beta strand","location":{"start":{"value":314,"modifier":"EXACT"},"end":{"value":321,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3ETH"}]},{"type":"Helix","location":{"start":{"value":325,"modifier":"EXACT"},"end":{"value":335,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3ETH"}]},{"type":"Helix","location":{"start":{"value":336,"modifier":"EXACT"},"end":{"value":338,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3ETH"}]},{"type":"Helix","location":{"start":{"value":341,"modifier":"EXACT"},"end":{"value":343,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3ETH"}]},{"type":"Helix","location":{"start":{"value":344,"modifier":"EXACT"},"end":{"value":353,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3ETH"}]}],"keywords":[{"id":"KW-0002","category":"Technical term","name":"3D-structure"},{"id":"KW-0067","category":"Ligand","name":"ATP-binding"},{"id":"KW-0903","category":"Technical term","name":"Direct protein sequencing"},{"id":"KW-0436","category":"Molecular function","name":"Ligase"},{"id":"KW-0547","category":"Ligand","name":"Nucleotide-binding"},{"id":"KW-0658","category":"Biological process","name":"Purine biosynthesis"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"}],"references":[{"referenceNumber":1,"citation":{"id":"2464576","citationType":"journal article","authors":["Tiedeman A.A.","Keyhani J.","Kamholz J.","Daum H.A. III","Gots J.S.","Smith J.M."],"citationCrossReferences":[{"database":"PubMed","id":"2464576"},{"database":"DOI","id":"10.1128/jb.171.1.205-212.1989"}],"title":"Nucleotide sequence analysis of the purEK operon encoding 5'-phosphoribosyl-5-aminoimidazole carboxylase of Escherichia coli K-12.","publicationDate":"1989","journal":"J. Bacteriol.","firstPage":"205","lastPage":"212","volume":"171"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"],"referenceComments":[{"value":"K12","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"2644189","citationType":"journal article","authors":["Watanabe W.","Sampei G.","Aiba A.","Mizobuchi K."],"citationCrossReferences":[{"database":"PubMed","id":"2644189"},{"database":"DOI","id":"10.1128/jb.171.1.198-204.1989"}],"title":"Identification and sequence analysis of Escherichia coli purE and purK genes encoding 5'-phosphoribosyl-5-amino-4-imidazole carboxylase for de novo purine biosynthesis.","publicationDate":"1989","journal":"J. Bacteriol.","firstPage":"198","lastPage":"204","volume":"171"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"]},{"referenceNumber":3,"citation":{"id":"CI-B1UJ6TNQ0SAS1","citationType":"submission","authors":["Chung E.","Allen E.","Araujo R.","Aparicio A.M.","Davis K.","Duncan M.","Federspiel N.","Hyman R.","Kalman S.","Komp C.","Kurdi O.","Lew H.","Lin D.","Namath A.","Oefner P.","Roberts D.","Schramm S.","Davis R.W."],"title":"Sequence of minutes 4-25 of Escherichia coli.","publicationDate":"JAN-1997","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / MG1655 / ATCC 47076","type":"STRAIN"}]},{"referenceNumber":4,"citation":{"id":"9278503","citationType":"journal article","authors":["Blattner F.R.","Plunkett G. III","Bloch C.A.","Perna N.T.","Burland V.","Riley M.","Collado-Vides J.","Glasner J.D.","Rode C.K.","Mayhew G.F.","Gregor J.","Davis N.W.","Kirkpatrick H.A.","Goeden M.A.","Rose D.J.","Mau B.","Shao Y."],"citationCrossReferences":[{"database":"PubMed","id":"9278503"},{"database":"DOI","id":"10.1126/science.277.5331.1453"}],"title":"The complete genome sequence of Escherichia coli K-12.","publicationDate":"1997","journal":"Science","firstPage":"1453","lastPage":"1462","volume":"277"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / MG1655 / ATCC 47076","type":"STRAIN"}]},{"referenceNumber":5,"citation":{"id":"16738553","citationType":"journal article","authors":["Hayashi K.","Morooka N.","Yamamoto Y.","Fujita K.","Isono K.","Choi S.","Ohtsubo E.","Baba T.","Wanner B.L.","Mori H.","Horiuchi T."],"citationCrossReferences":[{"database":"PubMed","id":"16738553"},{"database":"DOI","id":"10.1038/msb4100049"}],"title":"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.","publicationDate":"2006","journal":"Mol. Syst. Biol.","firstPage":"E1","lastPage":"E5","volume":"2"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / W3110 / ATCC 27325 / DSM 5911","type":"STRAIN"}]},{"referenceNumber":6,"citation":{"id":"1534690","citationType":"journal article","authors":["Meyer E.","Leonard N.J.","Bhat B.","Stubbe J.","Smith J.M."],"citationCrossReferences":[{"database":"PubMed","id":"1534690"},{"database":"DOI","id":"10.1021/bi00136a016"}],"title":"Purification and characterization of the purE, purK, and purC gene products: identification of a previously unrecognized energy requirement in the purine biosynthetic pathway.","publicationDate":"1992","journal":"Biochemistry","firstPage":"5022","lastPage":"5032","volume":"31"},"referencePositions":["PROTEIN SEQUENCE OF 1-20"]},{"referenceNumber":7,"citation":{"id":"8117684","citationType":"journal article","authors":["Mueller E.J.","Meyer E.","Rudolph J.","Davisson V.J.","Stubbe J."],"citationCrossReferences":[{"database":"PubMed","id":"8117684"},{"database":"DOI","id":"10.1021/bi00174a038"}],"title":"N5-carboxyaminoimidazole ribonucleotide: evidence for a new intermediate and two new enzymatic activities in the de novo purine biosynthetic pathway of Escherichia coli.","publicationDate":"1994","journal":"Biochemistry","firstPage":"2269","lastPage":"2278","volume":"33"},"referencePositions":["FUNCTION","CATALYTIC ACTIVITY","SUBSTRATE SPECIFICITY","PATHWAY"]},{"referenceNumber":8,"citation":{"id":"10569930","citationType":"journal article","authors":["Thoden J.B.","Kappock T.J.","Stubbe J.","Holden H.M."],"citationCrossReferences":[{"database":"PubMed","id":"10569930"},{"database":"DOI","id":"10.1021/bi991618s"}],"title":"Three-dimensional structure of N5-carboxyaminoimidazole ribonucleotide synthetase: a member of the ATP grasp protein superfamily.","publicationDate":"1999","journal":"Biochemistry","firstPage":"15480","lastPage":"15492","volume":"38"},"referencePositions":["X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) IN COMPLEX WITH ATP ANALOG","SUBUNIT"]},{"referenceNumber":9,"citation":{"id":"19053251","citationType":"journal article","authors":["Thoden J.B.","Holden H.M.","Firestine S.M."],"citationCrossReferences":[{"database":"PubMed","id":"19053251"},{"database":"DOI","id":"10.1021/bi801734z"}],"title":"Structural analysis of the active site geometry of N5-carboxyaminoimidazole ribonucleotide synthetase from Escherichia coli.","publicationDate":"2008","journal":"Biochemistry","firstPage":"13346","lastPage":"13353","volume":"47"},"referencePositions":["X-RAY CRYSTALLOGRAPHY (1.60 ANGSTROMS) IN COMPLEX WITH ATP","SUBUNIT"],"referenceComments":[{"value":"K12 / W3110 / ATCC 27325 / DSM 5911","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"X12982","properties":[{"key":"ProteinId","value":"CAA31421.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"M19657","properties":[{"key":"ProteinId","value":"AAA24450.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"U82664","properties":[{"key":"ProteinId","value":"AAB40275.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"U00096","properties":[{"key":"ProteinId","value":"AAC73624.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AP009048","properties":[{"key":"ProteinId","value":"BAE76299.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"JU0001","properties":[{"key":"EntryName","value":"DCECPK"}]},{"database":"RefSeq","id":"NP_415055.1","properties":[{"key":"NucleotideSequenceId","value":"NC_000913.3"}]},{"database":"RefSeq","id":"WP_000815571.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_CP009789.1"}]},{"database":"PDB","id":"1B6R","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.10 A"},{"key":"Chains","value":"A=1-355"}]},{"database":"PDB","id":"1B6S","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.50 A"},{"key":"Chains","value":"A/B/C/D=1-355"}]},{"database":"PDB","id":"3ETH","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.60 A"},{"key":"Chains","value":"A/B=1-355"}]},{"database":"PDB","id":"3ETJ","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.60 A"},{"key":"Chains","value":"A/B=1-355"}]},{"database":"PDBsum","id":"1B6R","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"1B6S","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"3ETH","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"3ETJ","properties":[{"key":"Description","value":"-"}]},{"database":"AlphaFoldDB","id":"P09029","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"P09029","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"4259875","properties":[{"key":"Interactions","value":"22"}]},{"database":"BioGRID","id":"849541","properties":[{"key":"Interactions","value":"9"}]},{"database":"FunCoup","id":"P09029","properties":[{"key":"Number of interactors","value":"568"}]},{"database":"IntAct","id":"P09029","properties":[{"key":"Interactions","value":"5"}]},{"database":"STRING","id":"511145.b0522","properties":[{"key":"Description","value":"-"}]},{"database":"jPOST","id":"P09029","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"511145-b0522","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblBacteria","id":"AAC73624","properties":[{"key":"ProteinId","value":"AAC73624"},{"key":"GeneId","value":"b0522"}]},{"database":"GeneID","id":"945153","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ecj:JW0511","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"eco:b0522","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ecoc:C3026_02560","properties":[{"key":"Description","value":"-"}]},{"database":"PATRIC","id":"fig|1411691.4.peg.1756","properties":[{"key":"GeneDesignation","value":"-"}]},{"database":"EchoBASE","id":"EB0789","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"COG0026","properties":[{"key":"ToxonomicScope","value":"Bacteria"}]},{"database":"HOGENOM","id":"CLU_011534_0_0_6","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"P09029","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"ITFDHEH","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"9804625at2","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"P09029","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"EcoCyc:PURK-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"MetaCyc:PURK-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"BRENDA","id":"6.3.4.18","properties":[{"key":"OrganismId","value":"2026"}]},{"database":"SABIO-RK","id":"P09029","properties":[{"key":"Description","value":"-"}]},{"database":"UniPathway","id":"UPA00074","properties":[{"key":"RectionId","value":"UER00942"}]},{"database":"EvolutionaryTrace","id":"P09029","properties":[{"key":"Description","value":"-"}]},{"database":"PRO","id":"PR:P09029","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000000625","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"AbasyAtlas","id":"P09029","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005829","properties":[{"key":"GoTerm","value":"C:cytosol"},{"key":"GoEvidenceType","value":"IDA:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"18304323"}]},{"database":"GO","id":"GO:0034028","properties":[{"key":"GoTerm","value":"F:5-(carboxyamino)imidazole ribonucleotide synthase activity"},{"key":"GoEvidenceType","value":"IDA:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"8117684"}]},{"database":"GO","id":"GO:0005524","properties":[{"key":"GoTerm","value":"F:ATP binding"},{"key":"GoEvidenceType","value":"IDA:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"19053251"},{"evidenceCode":"ECO:0000314","source":"PubMed","id":"8117684"}]},{"database":"GO","id":"GO:0046872","properties":[{"key":"GoTerm","value":"F:metal ion binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0004638","properties":[{"key":"GoTerm","value":"F:phosphoribosylaminoimidazole carboxylase activity"},{"key":"GoEvidenceType","value":"IDA:EcoliWiki"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"8117684"}]},{"database":"GO","id":"GO:0006189","properties":[{"key":"GoTerm","value":"P:'de novo' IMP biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0006144","properties":[{"key":"GoTerm","value":"P:purine nucleobase metabolic process"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"FunFam","id":"3.30.1490.20:FF:000021","properties":[{"key":"EntryName","value":"N5-carboxyaminoimidazole ribonucleotide synthase"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.30.470.20:FF:000034","properties":[{"key":"EntryName","value":"N5-carboxyaminoimidazole ribonucleotide synthase"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.40.50.20:FF:000017","properties":[{"key":"EntryName","value":"N5-carboxyaminoimidazole ribonucleotide synthase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.20","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.1490.20","properties":[{"key":"EntryName","value":"ATP-grasp fold, A domain"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.470.20","properties":[{"key":"EntryName","value":"ATP-grasp fold, B domain"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_01928","properties":[{"key":"EntryName","value":"PurK"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR011761","properties":[{"key":"EntryName","value":"ATP-grasp"}]},{"database":"InterPro","id":"IPR003135","properties":[{"key":"EntryName","value":"ATP-grasp_carboxylate-amine"}]},{"database":"InterPro","id":"IPR013815","properties":[{"key":"EntryName","value":"ATP_grasp_subdomain_1"}]},{"database":"InterPro","id":"IPR016185","properties":[{"key":"EntryName","value":"PreATP-grasp_dom_sf"}]},{"database":"InterPro","id":"IPR005875","properties":[{"key":"EntryName","value":"PurK"}]},{"database":"InterPro","id":"IPR040686","properties":[{"key":"EntryName","value":"PurK_C"}]},{"database":"InterPro","id":"IPR054350","properties":[{"key":"EntryName","value":"PurT/PurK_preATP-grasp"}]},{"database":"InterPro","id":"IPR011054","properties":[{"key":"EntryName","value":"Rudment_hybrid_motif"}]},{"database":"NCBIfam","id":"NF004678","properties":[{"key":"EntryName","value":"PRK06019.1-4"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"TIGR01161","properties":[{"key":"EntryName","value":"purK"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR11609:SF5","properties":[{"key":"EntryName","value":"PHOSPHORIBOSYLAMINOIMIDAZOLE CARBOXYLASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR11609","properties":[{"key":"EntryName","value":"PURINE BIOSYNTHESIS PROTEIN 6/7, PUR6/7"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF02222","properties":[{"key":"EntryName","value":"ATP-grasp"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF17769","properties":[{"key":"EntryName","value":"PurK_C"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF22660","properties":[{"key":"EntryName","value":"RS_preATP-grasp-like"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF56059","properties":[{"key":"EntryName","value":"Glutathione synthetase ATP-binding domain-like"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF52440","properties":[{"key":"EntryName","value":"PreATP-grasp domain"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF51246","properties":[{"key":"EntryName","value":"Rudiment single hybrid motif"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS50975","properties":[{"key":"EntryName","value":"ATP_GRASP"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQSVITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAERSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSGEVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELGYVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVVNNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEALIPLLPPEYASGVIWAQSKFG","length":355,"molWeight":39461,"crc64":"93464E111E29AD9C","md5":"B5C6BB96B8F2C6C0EFB07F936734EF5D"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"PATHWAY":1,"SUBUNIT":1,"SIMILARITY":1,"CAUTION":1},"countByFeatureType":{"Chain":1,"Domain":1,"Binding site":7,"Sequence conflict":1,"Beta strand":17,"Helix":22,"Turn":4},"uniParcId":"UPI0000132B1E"}}