{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P06278","secondaryAccessions":["Q45283","Q84I71"],"uniProtkbId":"AMY_BACLI","entryAudit":{"firstPublicDate":"1988-01-01","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"1988-01-01","entryVersion":174,"sequenceVersion":1},"annotationScore":5.0,"organism":{"scientificName":"Bacillus licheniformis","taxonId":1402,"lineage":["Bacteria","Bacillati","Bacillota","Bacilli","Caryophanales","Bacillaceae","Bacillus"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Alpha-amylase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11997021"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12915728"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"14632998"}],"value":"3.2.1.1"}]},"alternativeNames":[{"fullName":{"value":"1,4-alpha-D-glucan glucanohydrolase"}},{"fullName":{"value":"BLA"}}],"flag":"Precursor"},"genes":[{"geneName":{"value":"amyS"},"synonyms":[{"value":"amyL"}]}],"comments":[{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.","ecNumber":"3.2.1.1","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11997021"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12915728"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"14632998"}]}},{"commentType":"COFACTOR","cofactors":[{"name":"Ca(2+)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12540849"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9551551"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:29108"}}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12540849"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9551551"}],"value":"Binds 3 Ca(2+) ions per subunit."}]}},{"commentType":"COFACTOR","cofactors":[{"name":"Na(+)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12540849"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9551551"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:29101"}}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12540849"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9551551"}],"value":"Binds 1 sodium ion per subunit."}]}},{"commentType":"BIOPHYSICOCHEMICAL PROPERTIES","phDependence":{"texts":[{"value":"Active up to pH 11."}]},"temperatureDependence":{"texts":[{"value":"Active up to 100 degrees Celsius."}]}},{"texts":[{"value":"Monomer"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"14632998"}],"value":"Secreted","id":"SL-0243"}}]},{"texts":[{"value":"Used in the food industry for high temperature liquefaction of starch-containing mashes and in the detergent industry to remove starch. Sold under the name Termamyl by Novozymes"}],"commentType":"BIOTECHNOLOGY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the glycosyl hydrolase 13 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Signal","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":29,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"6172418"}]},{"type":"Chain","location":{"start":{"value":30,"modifier":"EXACT"},"end":{"value":512,"modifier":"EXACT"}},"description":"Alpha-amylase","featureId":"PRO_0000001332"},{"type":"Active site","location":{"start":{"value":260,"modifier":"EXACT"},"end":{"value":260,"modifier":"EXACT"}},"description":"Nucleophile"},{"type":"Active site","location":{"start":{"value":290,"modifier":"EXACT"},"end":{"value":290,"modifier":"EXACT"}},"description":"Proton donor"},{"type":"Binding site","location":{"start":{"value":133,"modifier":"EXACT"},"end":{"value":133,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29108"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12540849"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9551551"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"1BLI"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"1OB0"}],"ligand":{"name":"Ca(2+)","id":"ChEBI:CHEBI:29108","label":"1"}},{"type":"Binding site","location":{"start":{"value":190,"modifier":"EXACT"},"end":{"value":190,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29108"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12540849"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9551551"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"1BLI"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"1OB0"}],"ligand":{"name":"Ca(2+)","id":"ChEBI:CHEBI:29108","label":"2"}},{"type":"Binding site","location":{"start":{"value":190,"modifier":"EXACT"},"end":{"value":190,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29101"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12540849"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9551551"}],"ligand":{"name":"Na(+)","id":"ChEBI:CHEBI:29101"}},{"type":"Binding site","location":{"start":{"value":210,"modifier":"EXACT"},"end":{"value":210,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29108"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12540849"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9551551"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"1BLI"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"1OB0"}],"ligand":{"name":"Ca(2+)","id":"ChEBI:CHEBI:29108","label":"2"}},{"type":"Binding site","location":{"start":{"value":212,"modifier":"EXACT"},"end":{"value":212,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29108"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12540849"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9551551"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"1BLI"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"1OB0"}],"ligand":{"name":"Ca(2+)","id":"ChEBI:CHEBI:29108","label":"2"}},{"type":"Binding site","location":{"start":{"value":212,"modifier":"EXACT"},"end":{"value":212,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29101"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12540849"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9551551"}],"ligand":{"name":"Na(+)","id":"ChEBI:CHEBI:29101"}},{"type":"Binding site","location":{"start":{"value":223,"modifier":"EXACT"},"end":{"value":223,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29108"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12540849"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9551551"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"1BLI"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"1OB0"}],"ligand":{"name":"Ca(2+)","id":"ChEBI:CHEBI:29108","label":"1"}},{"type":"Binding site","location":{"start":{"value":223,"modifier":"EXACT"},"end":{"value":223,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29101"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12540849"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9551551"}],"ligand":{"name":"Na(+)","id":"ChEBI:CHEBI:29101"}},{"type":"Binding site","location":{"start":{"value":229,"modifier":"EXACT"},"end":{"value":229,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29108"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12540849"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9551551"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"1BLI"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"1OB0"}],"ligand":{"name":"Ca(2+)","id":"ChEBI:CHEBI:29108","label":"1"}},{"type":"Binding site","location":{"start":{"value":229,"modifier":"EXACT"},"end":{"value":229,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29101"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12540849"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9551551"}],"ligand":{"name":"Na(+)","id":"ChEBI:CHEBI:29101"}},{"type":"Binding site","location":{"start":{"value":231,"modifier":"EXACT"},"end":{"value":231,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29108"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12540849"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9551551"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"1BLI"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"1OB0"}],"ligand":{"name":"Ca(2+)","id":"ChEBI:CHEBI:29108","label":"2"}},{"type":"Binding site","location":{"start":{"value":233,"modifier":"EXACT"},"end":{"value":233,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29108"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12540849"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9551551"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"1BLI"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"1OB0"}],"ligand":{"name":"Ca(2+)","id":"ChEBI:CHEBI:29108","label":"2"}},{"type":"Binding site","location":{"start":{"value":264,"modifier":"EXACT"},"end":{"value":264,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29108"}],"evidences":[{"evidenceCode":"ECO:0007744","source":"PDB","id":"1BLI"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"1OB0"}],"ligand":{"name":"Ca(2+)","id":"ChEBI:CHEBI:29108","label":"1"}},{"type":"Binding site","location":{"start":{"value":329,"modifier":"EXACT"},"end":{"value":329,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29108"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12540849"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9551551"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"1BLI"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"1OB0"}],"ligand":{"name":"Ca(2+)","id":"ChEBI:CHEBI:29108","label":"3"}},{"type":"Binding site","location":{"start":{"value":331,"modifier":"EXACT"},"end":{"value":331,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29108"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12540849"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9551551"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"1BLI"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"1OB0"}],"ligand":{"name":"Ca(2+)","id":"ChEBI:CHEBI:29108","label":"3"}},{"type":"Binding site","location":{"start":{"value":435,"modifier":"EXACT"},"end":{"value":435,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29108"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12540849"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9551551"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"1BLI"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"1OB0"}],"ligand":{"name":"Ca(2+)","id":"ChEBI:CHEBI:29108","label":"3"}},{"type":"Binding site","location":{"start":{"value":436,"modifier":"EXACT"},"end":{"value":436,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29108"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12540849"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9551551"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"1BLI"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"1OB0"}],"ligand":{"name":"Ca(2+)","id":"ChEBI:CHEBI:29108","label":"3"}},{"type":"Binding site","location":{"start":{"value":459,"modifier":"EXACT"},"end":{"value":459,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29108"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12540849"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9551551"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"1BLI"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"1OB0"}],"ligand":{"name":"Ca(2+)","id":"ChEBI:CHEBI:29108","label":"3"}},{"type":"Site","location":{"start":{"value":357,"modifier":"EXACT"},"end":{"value":357,"modifier":"EXACT"}},"description":"Transition state stabilizer","evidences":[{"evidenceCode":"ECO:0000250"}]},{"type":"Mutagenesis","location":{"start":{"value":64,"modifier":"EXACT"},"end":{"value":64,"modifier":"EXACT"}},"description":"No effect on thermostability.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"2394736"}],"alternativeSequence":{"originalSequence":"H","alternativeSequences":["X"]}},{"type":"Mutagenesis","location":{"start":{"value":150,"modifier":"EXACT"},"end":{"value":150,"modifier":"EXACT"}},"description":"Decrease in thermostability.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10966804"}],"alternativeSequence":{"originalSequence":"D","alternativeSequences":["X"]}},{"type":"Mutagenesis","location":{"start":{"value":155,"modifier":"EXACT"},"end":{"value":155,"modifier":"EXACT"}},"description":"Decrease in thermostability.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10966804"}],"alternativeSequence":{"originalSequence":"N","alternativeSequences":["X"]}},{"type":"Mutagenesis","location":{"start":{"value":162,"modifier":"EXACT"},"end":{"value":162,"modifier":"EXACT"}},"description":"Increase in thermostability.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"2394736"}],"alternativeSequence":{"originalSequence":"H","alternativeSequences":["I","V"]}},{"type":"Mutagenesis","location":{"start":{"value":162,"modifier":"EXACT"},"end":{"value":162,"modifier":"EXACT"}},"description":"Great decrease in thermostability.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"2394736"}],"alternativeSequence":{"originalSequence":"H","alternativeSequences":["P"]}},{"type":"Mutagenesis","location":{"start":{"value":162,"modifier":"EXACT"},"end":{"value":162,"modifier":"EXACT"}},"description":"Great increase in thermostability; when associated with F-219; V-238; S-293 and Y-294.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"2394736"}],"alternativeSequence":{"originalSequence":"H","alternativeSequences":["V"]}},{"type":"Mutagenesis","location":{"start":{"value":175,"modifier":"EXACT"},"end":{"value":175,"modifier":"EXACT"}},"description":"No effect on thermostability.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10966804"}],"alternativeSequence":{"originalSequence":"R","alternativeSequences":["X"]}},{"type":"Mutagenesis","location":{"start":{"value":193,"modifier":"EXACT"},"end":{"value":193,"modifier":"EXACT"}},"description":"Loss of activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10966804"}],"alternativeSequence":{"originalSequence":"D","alternativeSequences":["A","C","E","H","R"]}},{"type":"Mutagenesis","location":{"start":{"value":201,"modifier":"EXACT"},"end":{"value":201,"modifier":"EXACT"}},"description":"Increase in thermostability.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10966804"}],"alternativeSequence":{"originalSequence":"N","alternativeSequences":["H","K"]}},{"type":"Mutagenesis","location":{"start":{"value":201,"modifier":"EXACT"},"end":{"value":201,"modifier":"EXACT"}},"description":"Great increase in thermostability.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10966804"}],"alternativeSequence":{"originalSequence":"N","alternativeSequences":["R"]}},{"type":"Mutagenesis","location":{"start":{"value":207,"modifier":"EXACT"},"end":{"value":207,"modifier":"EXACT"}},"description":"No effect on thermostability.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10966804"}],"alternativeSequence":{"originalSequence":"Q","alternativeSequences":["X"]}},{"type":"Mutagenesis","location":{"start":{"value":217,"modifier":"EXACT"},"end":{"value":217,"modifier":"EXACT"}},"description":"Great increase in thermostability.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10966804"}],"alternativeSequence":{"originalSequence":"N","alternativeSequences":["P"]}},{"type":"Mutagenesis","location":{"start":{"value":219,"modifier":"EXACT"},"end":{"value":219,"modifier":"EXACT"}},"description":"Great decrease in thermostability.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10966804"}],"alternativeSequence":{"originalSequence":"N","alternativeSequences":["A","E","Q","P","S"]}},{"type":"Mutagenesis","location":{"start":{"value":219,"modifier":"EXACT"},"end":{"value":219,"modifier":"EXACT"}},"description":"Great increase in thermostability. Great increase in thermostability; when associated with V-162; V-238; S-293 and Y-294.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10966804"}],"alternativeSequence":{"originalSequence":"N","alternativeSequences":["F"]}},{"type":"Mutagenesis","location":{"start":{"value":219,"modifier":"EXACT"},"end":{"value":219,"modifier":"EXACT"}},"description":"Increase in thermostability.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10966804"}],"alternativeSequence":{"originalSequence":"N","alternativeSequences":["L"]}},{"type":"Mutagenesis","location":{"start":{"value":221,"modifier":"EXACT"},"end":{"value":221,"modifier":"EXACT"}},"description":"Great decrease in thermostability.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10966804"}],"alternativeSequence":{"originalSequence":"N","alternativeSequences":["X"]}},{"type":"Mutagenesis","location":{"start":{"value":229,"modifier":"EXACT"},"end":{"value":229,"modifier":"EXACT"}},"description":"Great decrease in thermostability.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10966804"}],"alternativeSequence":{"originalSequence":"D","alternativeSequences":["X"]}},{"type":"Mutagenesis","location":{"start":{"value":233,"modifier":"EXACT"},"end":{"value":233,"modifier":"EXACT"}},"description":"Great decrease in thermostability.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10966804"}],"alternativeSequence":{"originalSequence":"D","alternativeSequences":["X"]}},{"type":"Mutagenesis","location":{"start":{"value":238,"modifier":"EXACT"},"end":{"value":238,"modifier":"EXACT"}},"description":"Increase in thermostability. Great increase in thermostability; when associated with V-162; F-219; S-293 and Y-294.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"8771184"}],"alternativeSequence":{"originalSequence":"A","alternativeSequences":["V"]}},{"type":"Mutagenesis","location":{"start":{"value":276,"modifier":"EXACT"},"end":{"value":276,"modifier":"EXACT"}},"description":"No effect on thermostability.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"2394736"}],"alternativeSequence":{"originalSequence":"H","alternativeSequences":["X"]}},{"type":"Mutagenesis","location":{"start":{"value":292,"modifier":"EXACT"},"end":{"value":292,"modifier":"EXACT"}},"description":"Decrease in activity; when associated with Y-315.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12915728"}],"alternativeSequence":{"originalSequence":"W","alternativeSequences":["L"]}},{"type":"Mutagenesis","location":{"start":{"value":293,"modifier":"EXACT"},"end":{"value":293,"modifier":"EXACT"}},"description":"Increase in thermostability; when associated with Y-294. Great increase in thermostability; when associated with V-162; F-219; V-238 and Y-294.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12736372"}],"alternativeSequence":{"originalSequence":"Q","alternativeSequences":["S"]}},{"type":"Mutagenesis","location":{"start":{"value":294,"modifier":"EXACT"},"end":{"value":294,"modifier":"EXACT"}},"description":"Increase in thermostability; when associated with S-293. Great increase in thermostability; when associated with V-162; F-219; V-238 and S-293.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12736372"}],"alternativeSequence":{"originalSequence":"N","alternativeSequences":["Y"]}},{"type":"Mutagenesis","location":{"start":{"value":298,"modifier":"EXACT"},"end":{"value":298,"modifier":"EXACT"}},"description":"Loss of activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10966804"}],"alternativeSequence":{"originalSequence":"A","alternativeSequences":["H","K","L","Q","R"]}},{"type":"Mutagenesis","location":{"start":{"value":300,"modifier":"EXACT"},"end":{"value":300,"modifier":"EXACT"}},"description":"No effect on thermostability.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10966804"}],"alternativeSequence":{"originalSequence":"E","alternativeSequences":["X"]}},{"type":"Mutagenesis","location":{"start":{"value":315,"modifier":"EXACT"},"end":{"value":315,"modifier":"EXACT"}},"description":"Decrease in activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12915728"}],"alternativeSequence":{"originalSequence":"V","alternativeSequences":["F"]}},{"type":"Mutagenesis","location":{"start":{"value":315,"modifier":"EXACT"},"end":{"value":315,"modifier":"EXACT"}},"description":"Increase in activity. Decrease in activity; when associated with L-292.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12915728"}],"alternativeSequence":{"originalSequence":"V","alternativeSequences":["Y"]}},{"type":"Mutagenesis","location":{"start":{"value":322,"modifier":"EXACT"},"end":{"value":322,"modifier":"EXACT"}},"description":"No effect on thermostability.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"2394736"}],"alternativeSequence":{"originalSequence":"H","alternativeSequences":["X"]}},{"type":"Mutagenesis","location":{"start":{"value":359,"modifier":"EXACT"},"end":{"value":359,"modifier":"EXACT"}},"description":"No effect on thermostability.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10966804"}],"alternativeSequence":{"originalSequence":"Q","alternativeSequences":["X"]}},{"type":"Mutagenesis","location":{"start":{"value":365,"modifier":"EXACT"},"end":{"value":365,"modifier":"EXACT"}},"description":"Loss of activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10966804"}],"alternativeSequence":{"originalSequence":"E","alternativeSequences":["H"]}},{"type":"Mutagenesis","location":{"start":{"value":435,"modifier":"EXACT"},"end":{"value":435,"modifier":"EXACT"}},"description":"No effect on thermostability.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"2394736"}],"alternativeSequence":{"originalSequence":"H","alternativeSequences":["X"]}},{"type":"Mutagenesis","location":{"start":{"value":479,"modifier":"EXACT"},"end":{"value":479,"modifier":"EXACT"}},"description":"No effect on thermostability.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"2394736"}],"alternativeSequence":{"originalSequence":"H","alternativeSequences":["X"]}},{"type":"Sequence conflict","location":{"start":{"value":4,"modifier":"EXACT"},"end":{"value":4,"modifier":"EXACT"}},"description":"in Ref. 4; no nucleotide entry","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"Q","alternativeSequences":["H"]}},{"type":"Sequence conflict","location":{"start":{"value":13,"modifier":"EXACT"},"end":{"value":13,"modifier":"EXACT"}},"description":"in Ref. 3; AAO26743 and 4; no nucleotide entry","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"T","alternativeSequences":["P"]}},{"type":"Sequence conflict","location":{"start":{"value":33,"modifier":"EXACT"},"end":{"value":33,"modifier":"EXACT"}},"description":"in Ref. 6; AAA22232","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"N","alternativeSequences":["I"]}},{"type":"Sequence conflict","location":{"start":{"value":38,"modifier":"EXACT"},"end":{"value":38,"modifier":"EXACT"}},"description":"in Ref. 8; AA sequence","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"Q","alternativeSequences":["Y"]}},{"type":"Sequence conflict","location":{"start":{"value":48,"modifier":"EXACT"},"end":{"value":48,"modifier":"EXACT"}},"description":"in Ref. 4; no nucleotide entry","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"G","alternativeSequences":["R"]}},{"type":"Sequence conflict","location":{"start":{"value":62,"modifier":"EXACT"},"end":{"value":64,"modifier":"EXACT"}},"description":"in Ref. 4; no nucleotide entry","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"AEH","alternativeSequences":["VED"]}},{"type":"Sequence conflict","location":{"start":{"value":68,"modifier":"EXACT"},"end":{"value":69,"modifier":"EXACT"}},"description":"in Ref. 4; no nucleotide entry","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"AV","alternativeSequences":["VA"]}},{"type":"Sequence conflict","location":{"start":{"value":72,"modifier":"EXACT"},"end":{"value":72,"modifier":"EXACT"}},"description":"in Ref. 4; no nucleotide entry","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"P","alternativeSequences":["S"]}},{"type":"Sequence conflict","location":{"start":{"value":81,"modifier":"EXACT"},"end":{"value":81,"modifier":"EXACT"}},"description":"in Ref. 3; AAO26743 and 4; no nucleotide entry","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"A","alternativeSequences":["D"]}},{"type":"Sequence conflict","location":{"start":{"value":105,"modifier":"EXACT"},"end":{"value":117,"modifier":"EXACT"}},"description":"in Ref. 4; no nucleotide entry","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{}},{"type":"Sequence conflict","location":{"start":{"value":117,"modifier":"EXACT"},"end":{"value":117,"modifier":"EXACT"}},"description":"in Ref. 3; AAO26743","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"K","alternativeSequences":["N"]}},{"type":"Sequence conflict","location":{"start":{"value":158,"modifier":"EXACT"},"end":{"value":158,"modifier":"EXACT"}},"description":"in Ref. 3; AAO26743","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"I","alternativeSequences":["T"]}},{"type":"Sequence conflict","location":{"start":{"value":162,"modifier":"EXACT"},"end":{"value":162,"modifier":"EXACT"}},"description":"in Ref. 3; AAO26743","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"H","alternativeSequences":["Q"]}},{"type":"Sequence conflict","location":{"start":{"value":163,"modifier":"EXACT"},"end":{"value":163,"modifier":"EXACT"}},"description":"in Ref. 2; AAA22240","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"R","alternativeSequences":["L"]}},{"type":"Sequence conflict","location":{"start":{"value":171,"modifier":"EXACT"},"end":{"value":171,"modifier":"EXACT"}},"description":"in Ref. 3; AAO26743","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"H","alternativeSequences":["Q"]}},{"type":"Sequence conflict","location":{"start":{"value":218,"modifier":"EXACT"},"end":{"value":218,"modifier":"EXACT"}},"description":"in Ref. 3; AAO26743","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"E","alternativeSequences":["V"]}},{"type":"Sequence conflict","location":{"start":{"value":237,"modifier":"EXACT"},"end":{"value":238,"modifier":"EXACT"}},"description":"in Ref. 3; AAO26743","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"VA","alternativeSequences":["AT"]}},{"type":"Sequence conflict","location":{"start":{"value":339,"modifier":"EXACT"},"end":{"value":339,"modifier":"EXACT"}},"description":"in Ref. 2; AAA22240 and 3; AAO26743","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"S","alternativeSequences":["G"]}},{"type":"Sequence conflict","location":{"start":{"value":347,"modifier":"EXACT"},"end":{"value":347,"modifier":"EXACT"}},"description":"in Ref. 3; AAO26743","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"L","alternativeSequences":["V"]}},{"type":"Sequence conflict","location":{"start":{"value":349,"modifier":"EXACT"},"end":{"value":349,"modifier":"EXACT"}},"description":"in Ref. 2; AAA22240","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"A","alternativeSequences":["S"]}},{"type":"Sequence conflict","location":{"start":{"value":385,"modifier":"EXACT"},"end":{"value":385,"modifier":"EXACT"}},"description":"in Ref. 3; AAO26743","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"S","alternativeSequences":["A"]}},{"type":"Sequence conflict","location":{"start":{"value":390,"modifier":"EXACT"},"end":{"value":390,"modifier":"EXACT"}},"description":"in Ref. 3; AAO26743","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"V","alternativeSequences":["I"]}},{"type":"Sequence conflict","location":{"start":{"value":401,"modifier":"EXACT"},"end":{"value":401,"modifier":"EXACT"}},"description":"in Ref. 3; AAO26743","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"D","alternativeSequences":["A"]}},{"type":"Sequence conflict","location":{"start":{"value":421,"modifier":"EXACT"},"end":{"value":421,"modifier":"EXACT"}},"description":"in Ref. 3; AAO26743","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"K","alternativeSequences":["I"]}},{"type":"Sequence conflict","location":{"start":{"value":464,"modifier":"EXACT"},"end":{"value":464,"modifier":"EXACT"}},"description":"in Ref. 3; AAO26743","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"A","alternativeSequences":["T"]}},{"type":"Sequence conflict","location":{"start":{"value":487,"modifier":"EXACT"},"end":{"value":488,"modifier":"EXACT"}},"description":"in Ref. 3; AAO26743","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"EP","alternativeSequences":["DS"]}},{"type":"Sequence conflict","location":{"start":{"value":493,"modifier":"EXACT"},"end":{"value":493,"modifier":"EXACT"}},"description":"in Ref. 3; AAO26743","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"S","alternativeSequences":["A"]}},{"type":"Beta strand","location":{"start":{"value":36,"modifier":"EXACT"},"end":{"value":38,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1VJS"}]},{"type":"Beta strand","location":{"start":{"value":46,"modifier":"EXACT"},"end":{"value":48,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1VJS"}]},{"type":"Helix","location":{"start":{"value":50,"modifier":"EXACT"},"end":{"value":56,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1VJS"}]},{"type":"Helix","location":{"start":{"value":58,"modifier":"EXACT"},"end":{"value":63,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1VJS"}]},{"type":"Beta strand","location":{"start":{"value":68,"modifier":"EXACT"},"end":{"value":70,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1VJS"}]},{"type":"Helix","location":{"start":{"value":71,"modifier":"EXACT"},"end":{"value":73,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1VJS"}]},{"type":"Beta strand","location":{"start":{"value":75,"modifier":"EXACT"},"end":{"value":79,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1OB0"}]},{"type":"Beta strand","location":{"start":{"value":86,"modifier":"EXACT"},"end":{"value":89,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1OB0"}]},{"type":"Beta strand","location":{"start":{"value":101,"modifier":"EXACT"},"end":{"value":103,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1VJS"}]},{"type":"Helix","location":{"start":{"value":109,"modifier":"EXACT"},"end":{"value":121,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1VJS"}]},{"type":"Beta strand","location":{"start":{"value":125,"modifier":"EXACT"},"end":{"value":130,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1VJS"}]},{"type":"Beta strand","location":{"start":{"value":133,"modifier":"EXACT"},"end":{"value":135,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1VJS"}]},{"type":"Beta strand","location":{"start":{"value":139,"modifier":"EXACT"},"end":{"value":149,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1VJS"}]},{"type":"Beta strand","location":{"start":{"value":151,"modifier":"EXACT"},"end":{"value":153,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1VJS"}]},{"type":"Beta strand","location":{"start":{"value":162,"modifier":"EXACT"},"end":{"value":170,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1VJS"}]},{"type":"Turn","location":{"start":{"value":173,"modifier":"EXACT"},"end":{"value":177,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1VJS"}]},{"type":"Helix","location":{"start":{"value":186,"modifier":"EXACT"},"end":{"value":188,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1VJS"}]},{"type":"Beta strand","location":{"start":{"value":189,"modifier":"EXACT"},"end":{"value":193,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1VJS"}]},{"type":"Turn","location":{"start":{"value":196,"modifier":"EXACT"},"end":{"value":199,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1VJS"}]},{"type":"Beta strand","location":{"start":{"value":204,"modifier":"EXACT"},"end":{"value":206,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1VJS"}]},{"type":"Turn","location":{"start":{"value":207,"modifier":"EXACT"},"end":{"value":209,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1BPL"}]},{"type":"Beta strand","location":{"start":{"value":213,"modifier":"EXACT"},"end":{"value":215,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1OB0"}]},{"type":"Helix","location":{"start":{"value":223,"modifier":"EXACT"},"end":{"value":226,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1VJS"}]},{"type":"Beta strand","location":{"start":{"value":228,"modifier":"EXACT"},"end":{"value":230,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1VJS"}]},{"type":"Helix","location":{"start":{"value":235,"modifier":"EXACT"},"end":{"value":252,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1VJS"}]},{"type":"Beta strand","location":{"start":{"value":256,"modifier":"EXACT"},"end":{"value":259,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1VJS"}]},{"type":"Helix","location":{"start":{"value":262,"modifier":"EXACT"},"end":{"value":264,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1VJS"}]},{"type":"Helix","location":{"start":{"value":267,"modifier":"EXACT"},"end":{"value":281,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1VJS"}]},{"type":"Beta strand","location":{"start":{"value":286,"modifier":"EXACT"},"end":{"value":289,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1VJS"}]},{"type":"Helix","location":{"start":{"value":296,"modifier":"EXACT"},"end":{"value":305,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1VJS"}]},{"type":"Turn","location":{"start":{"value":306,"modifier":"EXACT"},"end":{"value":308,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1VJS"}]},{"type":"Beta strand","location":{"start":{"value":310,"modifier":"EXACT"},"end":{"value":313,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1VJS"}]},{"type":"Helix","location":{"start":{"value":315,"modifier":"EXACT"},"end":{"value":326,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1VJS"}]},{"type":"Turn","location":{"start":{"value":327,"modifier":"EXACT"},"end":{"value":329,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1VJS"}]},{"type":"Helix","location":{"start":{"value":333,"modifier":"EXACT"},"end":{"value":337,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1VJS"}]},{"type":"Beta strand","location":{"start":{"value":338,"modifier":"EXACT"},"end":{"value":340,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1VJS"}]},{"type":"Helix","location":{"start":{"value":341,"modifier":"EXACT"},"end":{"value":344,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1VJS"}]},{"type":"Helix","location":{"start":{"value":346,"modifier":"EXACT"},"end":{"value":348,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1VJS"}]},{"type":"Beta strand","location":{"start":{"value":349,"modifier":"EXACT"},"end":{"value":353,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1BPL"}]},{"type":"Turn","location":{"start":{"value":356,"modifier":"EXACT"},"end":{"value":358,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1VJS"}]},{"type":"Helix","location":{"start":{"value":367,"modifier":"EXACT"},"end":{"value":369,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1VJS"}]},{"type":"Turn","location":{"start":{"value":370,"modifier":"EXACT"},"end":{"value":372,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1VJS"}]},{"type":"Helix","location":{"start":{"value":373,"modifier":"EXACT"},"end":{"value":382,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1VJS"}]},{"type":"Beta strand","location":{"start":{"value":383,"modifier":"EXACT"},"end":{"value":386,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1VJS"}]},{"type":"Beta strand","location":{"start":{"value":389,"modifier":"EXACT"},"end":{"value":391,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1VJS"}]},{"type":"Helix","location":{"start":{"value":392,"modifier":"EXACT"},"end":{"value":396,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1VJS"}]},{"type":"Beta strand","location":{"start":{"value":401,"modifier":"EXACT"},"end":{"value":404,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1VJS"}]},{"type":"Helix","location":{"start":{"value":405,"modifier":"EXACT"},"end":{"value":407,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1VJS"}]},{"type":"Helix","location":{"start":{"value":410,"modifier":"EXACT"},"end":{"value":422,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1VJS"}]},{"type":"Beta strand","location":{"start":{"value":428,"modifier":"EXACT"},"end":{"value":431,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1VJS"}]},{"type":"Beta strand","location":{"start":{"value":434,"modifier":"EXACT"},"end":{"value":442,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1VJS"}]},{"type":"Beta strand","location":{"start":{"value":453,"modifier":"EXACT"},"end":{"value":460,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1VJS"}]},{"type":"Beta strand","location":{"start":{"value":463,"modifier":"EXACT"},"end":{"value":468,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1VJS"}]},{"type":"Helix","location":{"start":{"value":471,"modifier":"EXACT"},"end":{"value":473,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1VJS"}]},{"type":"Beta strand","location":{"start":{"value":477,"modifier":"EXACT"},"end":{"value":480,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1VJS"}]},{"type":"Beta strand","location":{"start":{"value":495,"modifier":"EXACT"},"end":{"value":501,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1VJS"}]},{"type":"Beta strand","location":{"start":{"value":506,"modifier":"EXACT"},"end":{"value":510,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1VJS"}]}],"keywords":[{"id":"KW-0002","category":"Technical term","name":"3D-structure"},{"id":"KW-0106","category":"Ligand","name":"Calcium"},{"id":"KW-0119","category":"Biological process","name":"Carbohydrate metabolism"},{"id":"KW-0903","category":"Technical term","name":"Direct protein sequencing"},{"id":"KW-0326","category":"Molecular function","name":"Glycosidase"},{"id":"KW-0378","category":"Molecular function","name":"Hydrolase"},{"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"id":"KW-0964","category":"Cellular component","name":"Secreted"},{"id":"KW-0732","category":"Domain","name":"Signal"}],"references":[{"referenceNumber":1,"citation":{"id":"2418011","citationType":"journal article","authors":["Yuuki T.","Nomura T.","Tezuka H.","Tsuboi A.","Yamagata H.","Tsukagoshi N.","Udaka S."],"citationCrossReferences":[{"database":"PubMed","id":"2418011"},{"database":"DOI","id":"10.1093/oxfordjournals.jbchem.a135381"}],"title":"Complete nucleotide sequence of a gene coding for heat- and pH-stable alpha-amylase of Bacillus licheniformis: comparison of the amino acid sequences of three bacterial liquefying alpha-amylases deduced from the DNA sequences.","publicationDate":"1985","journal":"J. Biochem.","firstPage":"1147","lastPage":"1156","volume":"98"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"],"referenceComments":[{"value":"ATCC 27811 / BCRC 10494 / FERM P-1038","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"3009417","citationType":"journal article","authors":["Gray G.L.","Mainzer S.E.","Rey M.W.","Lamsa M.H.","Kindle K.L.","Carmona C.","Requadt C."],"citationCrossReferences":[{"database":"PubMed","id":"3009417"},{"database":"DOI","id":"10.1128/jb.166.2.635-643.1986"}],"title":"Structural genes encoding the thermophilic alpha-amylases of Bacillus stearothermophilus and Bacillus licheniformis.","publicationDate":"1986","journal":"J. Bacteriol.","firstPage":"635","lastPage":"643","volume":"166"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"]},{"referenceNumber":3,"citation":{"id":"14632998","citationType":"journal article","authors":["Shahhoseini M.","Ziaee A.A.","Ghaemi N."],"citationCrossReferences":[{"database":"PubMed","id":"14632998"},{"database":"DOI","id":"10.1046/j.1365-2672.2003.02082.x"}],"title":"Expression and secretion of an alpha-amylase gene from a native strain of Bacillus licheniformis in Escherichia coli by T7 promoter and putative signal peptide of the gene.","publicationDate":"2003","journal":"J. Appl. Microbiol.","firstPage":"1250","lastPage":"1254","volume":"95"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]","CATALYTIC ACTIVITY","SUBCELLULAR LOCATION"]},{"referenceNumber":4,"citation":{"id":"6334606","citationType":"journal article","authors":["Sibakov M.","Palva I."],"citationCrossReferences":[{"database":"PubMed","id":"6334606"},{"database":"DOI","id":"10.1111/j.1432-1033.1984.tb08594.x"}],"title":"Isolation and the 5'-end nucleotide sequence of Bacillus licheniformis alpha-amylase gene.","publicationDate":"1984","journal":"Eur. J. Biochem.","firstPage":"567","lastPage":"572","volume":"145"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-121"],"referenceComments":[{"value":"ATCC 14580 / DSM 13 / NBRC 12200 / NCIMB 9375 / NCTC 10341 / Gibson 46","type":"STRAIN"}]},{"referenceNumber":5,"citation":{"id":"6609154","citationType":"journal article","authors":["Stephens M.A.","Ortlepp S.A.","Ollington J.F.","McConnell D.J."],"citationCrossReferences":[{"database":"PubMed","id":"6609154"},{"database":"DOI","id":"10.1128/jb.158.1.369-372.1984"}],"title":"Nucleotide sequence of the 5' region of the Bacillus licheniformis alpha-amylase gene: comparison with the B. amyloliquefaciens gene.","publicationDate":"1984","journal":"J. Bacteriol.","firstPage":"369","lastPage":"372","volume":"158"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-104"]},{"referenceNumber":6,"citation":{"id":"2265757","citationType":"journal article","authors":["Jorgensen L.","Hansen C.K.","Poulsen G.B.","Diderichsen B."],"citationCrossReferences":[{"database":"PubMed","id":"2265757"},{"database":"DOI","id":"10.1016/0378-1119(90)90338-r"}],"title":"In vivo genetic engineering: homologous recombination as a tool for plasmid construction.","publicationDate":"1990","journal":"Gene","firstPage":"37","lastPage":"41","volume":"96"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-33"]},{"referenceNumber":7,"citation":{"id":"2540150","citationType":"journal article","authors":["Laoide B.M.","Chambliss G.H.","McConnell D.J."],"citationCrossReferences":[{"database":"PubMed","id":"2540150"},{"database":"DOI","id":"10.1128/jb.171.5.2435-2442.1989"}],"title":"Bacillus licheniformis alpha-amylase gene, amyL, is subject to promoter-independent catabolite repression in Bacillus subtilis.","publicationDate":"1989","journal":"J. Bacteriol.","firstPage":"2435","lastPage":"2442","volume":"171"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-29"]},{"referenceNumber":8,"citation":{"id":"6172418","citationType":"journal article","authors":["Kuhn H.","Fietzek P.P.","Lampen J.O."],"citationCrossReferences":[{"database":"PubMed","id":"6172418"},{"database":"DOI","id":"10.1128/jb.149.1.372-373.1982"}],"title":"N-terminal amino acid sequence of Bacillus licheniformis alpha-amylase: comparison with Bacillus amyloliquefaciens and Bacillus subtilis enzymes.","publicationDate":"1982","journal":"J. Bacteriol.","firstPage":"372","lastPage":"373","volume":"149"},"referencePositions":["PROTEIN SEQUENCE OF 30-47"]},{"referenceNumber":9,"citation":{"id":"11997021","citationType":"journal article","authors":["Kandra L.","Gyemant G.","Remenyik J.","Hovanszki G.","Liptak A."],"citationCrossReferences":[{"database":"PubMed","id":"11997021"},{"database":"DOI","id":"10.1016/s0014-5793(02)02649-2"}],"title":"Action pattern and subsite mapping of Bacillus licheniformis alpha-amylase (BLA) with modified maltooligosaccharide substrates.","publicationDate":"2002","journal":"FEBS Lett.","firstPage":"79","lastPage":"82","volume":"518"},"referencePositions":["MAPPING OF SUBSTRATE-BINDING SITE","CATALYTIC ACTIVITY"]},{"referenceNumber":10,"citation":{"id":"2394736","citationType":"journal article","authors":["Declerck N.","Joyet P.","Gaillardin C.","Masson J.-M."],"citationCrossReferences":[{"database":"PubMed","id":"2394736"},{"database":"DOI","id":"10.1016/s0021-9258(18)55421-1"}],"title":"Use of amber suppressors to investigate the thermostability of Bacillus licheniformis alpha-amylase. Amino acid replacements at 6 histidine residues reveal a critical position at His-133.","publicationDate":"1990","journal":"J. Biol. Chem.","firstPage":"15481","lastPage":"15488","volume":"265"},"referencePositions":["MUTAGENESIS OF HIS-64; HIS-162; HIS-276; HIS-322; HIS-435 AND HIS-479"],"referenceComments":[{"value":"ATCC 6598 / NRS 745","type":"STRAIN"}]},{"referenceNumber":11,"citation":{"id":"8771184","citationType":"journal article","authors":["Declerck N.","Joyet P.","Trosset J.Y.","Garnier J.","Gaillardin C."],"citationCrossReferences":[{"database":"PubMed","id":"8771184"},{"database":"DOI","id":"10.1093/protein/8.10.1029"}],"title":"Hyperthermostable mutants of Bacillus licheniformis alpha-amylase: multiple amino acid replacements and molecular modelling.","publicationDate":"1995","journal":"Protein Eng.","firstPage":"1029","lastPage":"1037","volume":"8"},"referencePositions":["MUTAGENESIS OF ALA-238"],"referenceComments":[{"value":"ATCC 6598 / NRS 745","type":"STRAIN"}]},{"referenceNumber":12,"citation":{"id":"10966804","citationType":"journal article","authors":["Declerck N.","Machius M.","Wiegand G.","Huber R.","Gaillardin C."],"citationCrossReferences":[{"database":"PubMed","id":"10966804"},{"database":"DOI","id":"10.1006/jmbi.2000.4025"}],"title":"Probing structural determinants specifying high thermostability in Bacillus licheniformis alpha-amylase.","publicationDate":"2000","journal":"J. Mol. Biol.","firstPage":"1041","lastPage":"1057","volume":"301"},"referencePositions":["MUTAGENESIS OF ASP-150; ASN-155; ARG-175; ASP-193; ASN-201; GLN-207; ASN-217; ASN-219; ASN-221; ASP-229; ASP-233; ALA-298; GLU-300; GLN-359 AND GLU-365"],"referenceComments":[{"value":"ATCC 6598 / NRS 745","type":"STRAIN"}]},{"referenceNumber":13,"citation":{"id":"12736372","citationType":"journal article","authors":["Declerck N.","Machius M.","Joyet P.","Wiegand G.","Huber R.","Gaillardin C."],"citationCrossReferences":[{"database":"PubMed","id":"12736372"},{"database":"DOI","id":"10.1093/proeng/gzg032"}],"title":"Hyperthermostabilization of Bacillus licheniformis alpha-amylase and modulation of its stability over a 50 degrees C temperature range.","publicationDate":"2003","journal":"Protein Eng.","firstPage":"287","lastPage":"293","volume":"16"},"referencePositions":["MUTAGENESIS OF GLN-293 AND ASN-294"],"referenceComments":[{"value":"ATCC 6598 / NRS 745","type":"STRAIN"}]},{"referenceNumber":14,"citation":{"id":"12915728","citationType":"journal article","authors":["Rivera M.H.","Lopez-Munguia A.","Soberon X.","Saab-Rincon G."],"citationCrossReferences":[{"database":"PubMed","id":"12915728"},{"database":"DOI","id":"10.1093/protein/gzg060"}],"title":"Alpha-amylase from Bacillus licheniformis mutants near to the catalytic site: effects on hydrolytic and transglycosylation activity.","publicationDate":"2003","journal":"Protein Eng.","firstPage":"505","lastPage":"514","volume":"16"},"referencePositions":["CATALYTIC ACTIVITY","MUTAGENESIS OF TRP-292 AND VAL-315"],"referenceComments":[{"value":"ATCC 27811 / BCRC 10494 / FERM P-1038","type":"STRAIN"}]},{"referenceNumber":15,"citation":{"id":"7877175","citationType":"journal article","authors":["Machius M.","Wiegand G.","Huber R."],"citationCrossReferences":[{"database":"PubMed","id":"7877175"},{"database":"DOI","id":"10.1006/jmbi.1994.0106"}],"title":"Crystal structure of calcium-depleted Bacillus licheniformis alpha-amylase at 2.2-A resolution.","publicationDate":"1995","journal":"J. Mol. Biol.","firstPage":"545","lastPage":"559","volume":"246"},"referencePositions":["X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 30-512"],"referenceComments":[{"value":"ATCC 27811 / BCRC 10494 / FERM P-1038","type":"STRAIN"}]},{"referenceNumber":16,"citation":{"id":"9551551","citationType":"journal article","authors":["Machius M.","Declerck N.","Huber R.","Wiegand G."],"citationCrossReferences":[{"database":"PubMed","id":"9551551"},{"database":"DOI","id":"10.1016/s0969-2126(98)00032-x"}],"title":"Activation of Bacillus licheniformis alpha-amylase through a disorder-->order transition of the substrate-binding site mediated by a calcium-sodium-calcium metal triad.","publicationDate":"1998","journal":"Structure","firstPage":"281","lastPage":"292","volume":"6"},"referencePositions":["X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS) OF 30-512 IN COMPLEX WITH CALCIUM AND SODIUM","COFACTOR"]},{"referenceNumber":17,"citation":{"id":"10924103","citationType":"journal article","authors":["Brzozowski A.M.","Lawson D.M.","Turkenburg J.P.","Bisgaard-Frantzen H.","Svendsen A.","Borchert T.V.","Dauter Z.","Wilson K.S.","Davies G.J."],"citationCrossReferences":[{"database":"PubMed","id":"10924103"},{"database":"DOI","id":"10.1021/bi0000317"}],"title":"Structural analysis of a chimeric bacterial alpha-amylase. High-resolution analysis of native and ligand complexes.","publicationDate":"2000","journal":"Biochemistry","firstPage":"9099","lastPage":"9107","volume":"39"},"referencePositions":["X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 330-512"]},{"referenceNumber":18,"citation":{"id":"12540849","citationType":"journal article","authors":["Machius M.","Declerck N.","Huber R.","Wiegand G."],"citationCrossReferences":[{"database":"PubMed","id":"12540849"},{"database":"DOI","id":"10.1074/jbc.m212618200"}],"title":"Kinetic stabilization of Bacillus licheniformis alpha-amylase through introduction of hydrophobic residues at the surface.","publicationDate":"2003","journal":"J. Biol. Chem.","firstPage":"11546","lastPage":"11553","volume":"278"},"referencePositions":["X-RAY CRYSTALLOGRAPHY (1.83 ANGSTROMS) OF 30-512 OF MUTANT VAL-162/PHE-219/VAL-238/SER-293/TYR-294 IN COMPLEX WITH CALCIUM AND SODIUM","COFACTOR"],"referenceComments":[{"value":"ATCC 6598 / NRS 745","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"X03236","properties":[{"key":"ProteinId","value":"CAA26981.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"M38570","properties":[{"key":"ProteinId","value":"AAA22226.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"M13256","properties":[{"key":"ProteinId","value":"AAA22240.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AF438149","properties":[{"key":"ProteinId","value":"AAO26743.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"K01984","properties":[{"key":"ProteinId","value":"AAA22193.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"M62637","properties":[{"key":"ProteinId","value":"AAA22232.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"M26412","properties":[{"key":"ProteinId","value":"AAA22237.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"A91997","properties":[{"key":"EntryName","value":"ALBSL"}]},{"database":"RefSeq","id":"WP_017474613.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_UAQA01000026.1"}]},{"database":"RefSeq","id":"WP_025807921.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_BOQW01000006.1"}]},{"database":"PDB","id":"1BLI","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.90 A"},{"key":"Chains","value":"A=30-512"}]},{"database":"PDB","id":"1BPL","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.20 A"},{"key":"Chains","value":"A=30-218, B=219-512"}]},{"database":"PDB","id":"1E3X","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.90 A"},{"key":"Chains","value":"A=330-512"}]},{"database":"PDB","id":"1E3Z","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.93 A"},{"key":"Chains","value":"A=330-512"}]},{"database":"PDB","id":"1E40","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.20 A"},{"key":"Chains","value":"A=330-512"}]},{"database":"PDB","id":"1E43","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.70 A"},{"key":"Chains","value":"A=330-512"}]},{"database":"PDB","id":"1OB0","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.83 A"},{"key":"Chains","value":"A=30-512"}]},{"database":"PDB","id":"1VJS","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.70 A"},{"key":"Chains","value":"A=30-512"}]},{"database":"PDBsum","id":"1BLI","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"1BPL","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"1E3X","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"1E3Z","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"1E40","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"1E43","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"1OB0","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"1VJS","properties":[{"key":"Description","value":"-"}]},{"database":"AlphaFoldDB","id":"P06278","properties":[{"key":"Description","value":"-"}]},{"database":"PCDDB","id":"P06278","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"P06278","properties":[{"key":"Description","value":"-"}]},{"database":"BindingDB","id":"P06278","properties":[{"key":"Description","value":"-"}]},{"database":"ChEMBL","id":"CHEMBL4215","properties":[{"key":"Description","value":"-"}]},{"database":"Allergome","id":"8255","properties":[{"key":"AllergenName","value":"Bac li aA"}]},{"database":"CAZy","id":"GH13","properties":[{"key":"FamilyName","value":"Glycoside Hydrolase Family 13"}]},{"database":"BRENDA","id":"3.2.1.1","properties":[{"key":"OrganismId","value":"669"}]},{"database":"EvolutionaryTrace","id":"P06278","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005576","properties":[{"key":"GoTerm","value":"C:extracellular region"},{"key":"GoEvidenceType","value":"EXP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"14632998"}]},{"database":"GO","id":"GO:0004556","properties":[{"key":"GoTerm","value":"F:alpha-amylase activity"},{"key":"GoEvidenceType","value":"IDA:CACAO"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"2540150"}]},{"database":"GO","id":"GO:0005509","properties":[{"key":"GoTerm","value":"F:calcium ion binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0005975","properties":[{"key":"GoTerm","value":"P:carbohydrate metabolic process"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"CDD","id":"cd11318","properties":[{"key":"EntryName","value":"AmyAc_bac_fung_AmyA"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"2.40.30.140:FF:000002","properties":[{"key":"EntryName","value":"Alpha-amylase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.40.30.140","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.20.20.80","properties":[{"key":"EntryName","value":"Glycosidases"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.60.40.1180","properties":[{"key":"EntryName","value":"Golgi alpha-mannosidase II"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR013776","properties":[{"key":"EntryName","value":"A-amylase_thermo"}]},{"database":"InterPro","id":"IPR015237","properties":[{"key":"EntryName","value":"Alpha-amylase_C_pro"}]},{"database":"InterPro","id":"IPR006047","properties":[{"key":"EntryName","value":"GH13_cat_dom"}]},{"database":"InterPro","id":"IPR017853","properties":[{"key":"EntryName","value":"GH_hydrolase_sf"}]},{"database":"InterPro","id":"IPR013780","properties":[{"key":"EntryName","value":"Glyco_hydro_b"}]},{"database":"NCBIfam","id":"NF006968","properties":[{"key":"EntryName","value":"PRK09441.1-1"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"NF006969","properties":[{"key":"EntryName","value":"PRK09441.1-2"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"NF006972","properties":[{"key":"EntryName","value":"PRK09441.1-5"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR43447","properties":[{"key":"EntryName","value":"ALPHA-AMYLASE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF09154","properties":[{"key":"EntryName","value":"Alpha-amy_C_pro"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00128","properties":[{"key":"EntryName","value":"Alpha-amylase"},{"key":"MatchStatus","value":"1"}]},{"database":"PIRSF","id":"PIRSF001021","properties":[{"key":"EntryName","value":"Alph-amls_thrmst"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00642","properties":[{"key":"EntryName","value":"Aamy"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF51445","properties":[{"key":"EntryName","value":"(Trans)glycosidases"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF51011","properties":[{"key":"EntryName","value":"Glycosyl hydrolase domain"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MKQQKRLYARLLTLLFALIFLLPHSAAAAANLNGTLMQYFEWYMPNDGQHWKRLQNDSAYLAEHGITAVWIPPAYKGTSQADVGYGAYDLYDLGEFHQKGTVRTKYGTKGELQSAIKSLHSRDINVYGDVVINHKGGADATEDVTAVEVDPADRNRVISGEHRIKAWTHFHFPGRGSTYSDFKWHWYHFDGTDWDESRKLNRIYKFQGKAWDWEVSNENGNYDYLMYADIDYDHPDVAAEIKRWGTWYANELQLDGFRLDAVKHIKFSFLRDWVNHVREKTGKEMFTVAEYWQNDLGALENYLNKTNFNHSVFDVPLHYQFHAASTQGGGYDMRKLLNSTVVSKHPLKAVTFVDNHDTQPGQSLESTVQTWFKPLAYAFILTRESGYPQVFYGDMYGTKGDSQREIPALKHKIEPILKARKQYAYGAQHDYFDHHDIVGWTREGDSSVANSGLAALITDGPGGAKRMYVGRQNAGETWHDITGNRSEPVVINSEGWGEFHVNGGSVSIYVQR","length":512,"molWeight":58549,"crc64":"D8BB77759CD4C482","md5":"0A9397999C4D32EC8E67BC7E46E9B455"},"extraAttributes":{"countByCommentType":{"CATALYTIC ACTIVITY":1,"COFACTOR":2,"BIOPHYSICOCHEMICAL PROPERTIES":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"BIOTECHNOLOGY":1,"SIMILARITY":1},"countByFeatureType":{"Signal":1,"Chain":1,"Active site":2,"Binding site":18,"Site":1,"Mutagenesis":32,"Sequence conflict":27,"Beta strand":30,"Helix":20,"Turn":7},"uniParcId":"UPI0000125AAD"}}