{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P03781","secondaryAccessions":["Q6WYR0"],"uniProtkbId":"NUCK_BPT7","entryAudit":{"firstPublicDate":"1986-07-21","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"1986-07-21","entryVersion":79,"sequenceVersion":1},"annotationScore":5.0,"organism":{"scientificName":"Escherichia phage T7","commonName":"Bacteriophage T7","taxonId":10760,"lineage":["Viruses","Duplodnaviria","Heunggongvirae","Uroviricota","Caudoviricetes","Autographivirales","Autotranscriptaviridae","Studiervirinae","Teseptimavirus","Teseptimavirus T7"]},"organismHosts":[{"scientificName":"Escherichia coli","taxonId":562}],"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Nucleotide kinase gp1.7"}},"alternativeNames":[{"fullName":{"value":"Gene product 1.7"},"shortNames":[{"value":"Gp1.7"}],"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20497505"}],"value":"2.7.4.-"}]},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"20497505"}],"value":"Nucleoside monophosphate kinase"}}]},"genes":[{"orderedLocusNames":[{"value":"1.7"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18599435"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20497505"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"33157039"}],"value":"Nucleoside monophosphate kinase that catalyzes the phosphorylation of dGMP and dTMP to dGDP and dTDP, respectively, by using either GTP, dGTP or dTTP as the phosphate donor (PubMed:20497505). Other dNTPs and NTPs (including ATP, CTP, UTP, dATP and dCTP) cannot serve as phosphate donors in the conversion of dTMP to dTDP, and dAMP, dCMP and dUMP cannot be used as acceptor substrates (PubMed:20497505). Confers sensitivity of phage T7 growth to dideoxythymidine (ddT), due to its ability to efficiently catalyze the conversion of dideoxythymidylate (ddTMP) to ddTDP, which is finally converted to ddTTP where it is incorporated as a chain-terminating nucleotide into replicating T7 DNA (PubMed:18599435, PubMed:20497505). A double mutation in this protein and the RecBCD inhibitor gp5.9 protein allows phage to overcome the retron Ec48 bacteriophage defense system. This protein alone when overexpressed in E.coli does not cause growth arrest; Y128C may be a silent mutation (PubMed:33157039)"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"dGMP + GTP = dGDP + GDP","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:62136"},{"database":"ChEBI","id":"CHEBI:37565"},{"database":"ChEBI","id":"CHEBI:57673"},{"database":"ChEBI","id":"CHEBI:58189"},{"database":"ChEBI","id":"CHEBI:58595"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20497505"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:62137"},"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"20497505"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"dTMP + GTP = dTDP + 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phosphorylation of dGMP with GTP. kcat is 5.7 sec(-1) for the phosphorylation of dGMP with dGTP. kcat is 5.1 sec(-1) for the phosphorylation of dGMP with dTTP. kcat is 1.5 sec(-1) for the phosphorylation of dTMP with GTP. kcat is 4.5 sec(-1) for the phosphorylation of dTMP with dGTP. kcat is 3.3 sec(-1) for the phosphorylation of dTMP with dTTP."}]}},"phDependence":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20497505"}],"value":"Optimum pH is 7-7.5."}]}},{"commentType":"BIOPHYSICOCHEMICAL PROPERTIES","molecule":"Isoform Small gp1.7","kineticParameters":{"michaelisConstants":[{"constant":20.0,"unit":"uM","substrate":"dGMP (in the presence of GTP)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20497505"}]},{"constant":34.0,"unit":"uM","substrate":"dGMP (in the presence of dGTP)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20497505"}]},{"constant":37.0,"unit":"uM","substrate":"dGMP (in the presence of 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phosphorylation of dTMP with dTTP."}]}},"phDependence":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20497505"}],"value":"Optimum pH is 7-7.5."}]}},{"texts":[{"value":"Dodecamer"}],"commentType":"SUBUNIT"},{"commentType":"ALTERNATIVE PRODUCTS","events":["Alternative initiation"],"isoforms":[{"name":{"value":"Large gp1.7"},"isoformIds":["P03781-1"],"isoformSequenceStatus":"Displayed"},{"name":{"value":"Small gp1.7"},"isoformIds":["P03781-2"],"sequenceIds":["VSP_053707"],"isoformSequenceStatus":"Described"}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Produced by alternative initiation at Met-42 of isoform Small gp1.7"}],"commentType":"MISCELLANEOUS","molecule":"Isoform Small gp1.7"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":196,"modifier":"EXACT"}},"description":"Nucleotide kinase 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