{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P03594","uniProtkbId":"RDRP_BMV","entryAudit":{"firstPublicDate":"1986-07-21","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"1986-07-21","entryVersion":101,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Brome mosaic virus","commonName":"BMV","taxonId":12302,"lineage":["Viruses","Riboviria","Orthornavirae","Kitrinoviricota","Alsuviricetes","Martellivirales","Bromoviridae","Bromovirus","Bromovirus BMV"]},"organismHosts":[{"scientificName":"Bromus inermis","commonName":"Smooth brome grass","synonyms":["Bromopsis inermis"],"taxonId":15371}],"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"RNA-directed RNA polymerase 2a"},"shortNames":[{"value":"protein 2a"}],"ecNumbers":[{"value":"2.7.7.48"}]}},"genes":[{"orfNames":[{"value":"ORF2a"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"RNA-dependent RNA polymerase which replicates the viral genome composed of 3 RNA segments, RNA1, RNA2 and RNA3"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:21248"},{"database":"Rhea","id":"RHEA-COMP:14527"},{"database":"Rhea","id":"RHEA-COMP:17342"},{"database":"ChEBI","id":"CHEBI:33019"},{"database":"ChEBI","id":"CHEBI:61557"},{"database":"ChEBI","id":"CHEBI:140395"}],"ecNumber":"2.7.7.48","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00539"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"19325881"}],"value":"Interacts with replication protein 1a"}],"commentType":"SUBUNIT"},{"commentType":"INTERACTION","interactions":[{"interactantOne":{"uniProtKBAccession":"P03594","intActId":"EBI-15874357"},"interactantTwo":{"uniProtKBAccession":"P03588","geneName":"ORF1a","intActId":"EBI-15874242"},"numberOfExperiments":2,"organismDiffer":false}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the ssRNA positive-strand viruses RNA-directed RNA polymerase family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":822,"modifier":"EXACT"}},"description":"RNA-directed RNA polymerase 2a","featureId":"PRO_0000083269"},{"type":"Domain","location":{"start":{"value":459,"modifier":"EXACT"},"end":{"value":572,"modifier":"EXACT"}},"description":"RdRp catalytic","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00539"}]}],"keywords":[{"id":"KW-0547","category":"Ligand","name":"Nucleotide-binding"},{"id":"KW-0548","category":"Molecular function","name":"Nucleotidyltransferase"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0696","category":"Molecular function","name":"RNA-directed RNA polymerase"},{"id":"KW-0808","category":"Molecular function","name":"Transferase"},{"id":"KW-0693","category":"Biological process","name":"Viral RNA replication"}],"references":[{"referenceNumber":1,"citation":{"id":"6694215","citationType":"journal article","authors":["Ahlquist P.","Dasgupta R.","Kaesberg P."],"citationCrossReferences":[{"database":"PubMed","id":"6694215"},{"database":"DOI","id":"10.1016/s0022-2836(84)80012-1"}],"title":"Nucleotide sequence of the brome mosaic virus genome and its implications for viral replication.","publicationDate":"1984","journal":"J. Mol. Biol.","firstPage":"369","lastPage":"383","volume":"172"},"referencePositions":["NUCLEOTIDE SEQUENCE"]},{"referenceNumber":2,"citation":{"id":"19325881","citationType":"journal article","authors":["Liu L.","Westler W.M.","den Boon J.A.","Wang X.","Diaz A.","Steinberg H.A.","Ahlquist P."],"citationCrossReferences":[{"database":"PubMed","id":"19325881"},{"database":"DOI","id":"10.1371/journal.ppat.1000351"}],"title":"An amphipathic alpha-helix controls multiple roles of brome mosaic virus protein 1a in RNA replication complex assembly and function.","publicationDate":"2009","journal":"PLoS Pathog.","firstPage":"E1000351","lastPage":"E1000351","volume":"5"},"referencePositions":["INTERACTION WITH REPLICATION PROTEIN 1A"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"X01678","properties":[{"key":"ProteinId","value":"CAA25834.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Unassigned_RNA"}]},{"database":"PIR","id":"A04202","properties":[{"key":"EntryName","value":"P2BVA"}]},{"database":"RefSeq","id":"NP_041197.1","properties":[{"key":"NucleotideSequenceId","value":"NC_002027.1"}]},{"database":"AlphaFoldDB","id":"P03594","properties":[{"key":"Description","value":"-"}]},{"database":"DIP","id":"DIP-59379N","properties":[{"key":"Description","value":"-"}]},{"database":"IntAct","id":"P03594","properties":[{"key":"Interactions","value":"1"}]},{"database":"GeneID","id":"962145","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"vg:962145","properties":[{"key":"Description","value":"-"}]},{"database":"OrthoDB","id":"1928at10239","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000001649","properties":[{"key":"Component","value":"Genome"}]},{"database":"GO","id":"GO:0030430","properties":[{"key":"GoTerm","value":"C:host cell cytoplasm"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"22318148"}]},{"database":"GO","id":"GO:0000166","properties":[{"key":"GoTerm","value":"F:nucleotide binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-KW"}]},{"database":"GO","id":"GO:0003723","properties":[{"key":"GoTerm","value":"F:RNA binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0003968","properties":[{"key":"GoTerm","value":"F:RNA-directed RNA polymerase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-KW"}]},{"database":"GO","id":"GO:0006351","properties":[{"key":"GoTerm","value":"P:DNA-templated transcription"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0039690","properties":[{"key":"GoTerm","value":"P:positive stranded viral RNA replication"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"22318148"}]},{"database":"CDD","id":"cd23252","properties":[{"key":"EntryName","value":"Bromoviridae_RdRp"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR007610","properties":[{"key":"EntryName","value":"BBMV_Gp1_N"}]},{"database":"InterPro","id":"IPR047309","properties":[{"key":"EntryName","value":"Bromoviridae_RdRp"}]},{"database":"InterPro","id":"IPR043502","properties":[{"key":"EntryName","value":"DNA/RNA_pol_sf"}]},{"database":"InterPro","id":"IPR018228","properties":[{"key":"EntryName","value":"DNase_TatD-rel_CS"}]},{"database":"InterPro","id":"IPR001788","properties":[{"key":"EntryName","value":"RNA-dep_RNA_pol_alsuvir"}]},{"database":"InterPro","id":"IPR007094","properties":[{"key":"EntryName","value":"RNA-dir_pol_PSvirus"}]},{"database":"Pfam","id":"PF04522","properties":[{"key":"EntryName","value":"BBMV_Gp1_N"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00978","properties":[{"key":"EntryName","value":"RdRP_2"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF56672","properties":[{"key":"EntryName","value":"DNA/RNA polymerases"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS50507","properties":[{"key":"EntryName","value":"RDRP_SSRNA_POS"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MSSKTWDDDFVRQVPSFQWIIDQSLEDEVEAASLQVQEPADGVAIDGSLASFKLAIAPLEIGGVFDPPFDRVRWGSICDTVQQMVQQFTDRPLIPQAEMARMLYLDIPGSFVLEDEIDDWYPEDTSDGYGVSFAADEDHASDLKLASDSSNCEIEEVRVTGDTPKELTLGDRYMGIDEEFQTTNTDYDITLQIMNPIEHRVSRVIDTHCHPDNPDISTGPIYMERVSLARTEATSHSILPTHAYFDDSYHQALVENGDYSMDFDRIRLKQSDVDWYRDPDKYFQPKMNIGSAQRRVGTQKEVLTALKKRNADVPEMGDAINMKDTAKAIAKRFRSTFLNVDGEDCLRASMDVMTKCLEYHKKWGKHMDLQGVNVAAETDLCRYQHMLKSDVKPVVTDTLHLERAVAATITFHSKGVTSNFSPFFTACFEKLSLALKSRFIVPIGKISSLELKNVRLNNRYFLEADLSKFDKSQGELHLEFQREILLALGFPAPLTNWWSDFHRDSYLSDPHAKVGMSVSFQRRTGDAFTYFGNTLVTMAMIAYASDLSDCDCAIFSGDDSLIISKVKPVLDTDMFTSLFNMEIKVMDPSVPYVCSKFLVETEMGNLVSVPDPLREIQRLAKRKILRDEQMLRAHFVSFCDRMKFINQLDEKMITTLCHFVYLKYGKEKPWIFEEVRAALAAFSLYSENFLRFSDCYCTEGIRVYQMSDPVCKFKRTTEERKTDGDWFHNWKNPKFPGVLDKVYRTIGIYSSDCSTKELPVKRIGRLHEALERESLKLANDRRTTQRLKKKVDDYATGRGGLTSVDALLVKSHCETFKPSDLR","length":822,"molWeight":93927,"crc64":"C13B99016B2C54AC","md5":"1442A53CDACF462402EDA4E8A78DD226"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"SUBUNIT":1,"INTERACTION":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Domain":1},"uniParcId":"UPI0000137FE5"}}