{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P03210","secondaryAccessions":["Q777E2"],"uniProtkbId":"BRRF2_EBVB9","entryAudit":{"firstPublicDate":"1986-07-21","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"1986-07-21","entryVersion":73,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Epstein-Barr virus (strain B95-8)","commonName":"HHV-4","synonyms":["Human herpesvirus 4"],"taxonId":10377,"lineage":["Viruses","Duplodnaviria","Heunggongvirae","Peploviricota","Herviviricetes","Herpesvirales","Orthoherpesviridae","Gammaherpesvirinae","Lymphocryptovirus","Lymphocryptovirus humangamma4","Epstein-Barr virus"]},"organismHosts":[{"scientificName":"Homo sapiens","commonName":"Human","taxonId":9606}],"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Tegument protein BRRF2"}}},"genes":[{"orfNames":[{"value":"BRRF2"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"41073405"}],"value":"Plays a role in the inhibition of the dsDNA-triggered induction of downstream antiviral genes by interacting with and inhibiting host cGAS, limiting cGAS-DNA phase separation"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"41073405"}],"value":"Interacts with host CGAS; this interaction inhibits the enzymatic activity of cGAS by interfering with the interaction of cGAS with dsDNA"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"41073405"}],"value":"Virion tegument","id":"SL-0273"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28197146"}],"value":"Host early endosome","id":"SL-0461"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"41073405"}],"value":"Secreted","id":"SL-0243"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the lymphocryptovirus BRRF2 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":537,"modifier":"EXACT"}},"description":"Tegument protein BRRF2","featureId":"PRO_0000116260"},{"type":"Region","location":{"start":{"value":321,"modifier":"EXACT"},"end":{"value":366,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":378,"modifier":"EXACT"},"end":{"value":398,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":414,"modifier":"EXACT"},"end":{"value":466,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":486,"modifier":"EXACT"},"end":{"value":537,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":334,"modifier":"EXACT"},"end":{"value":347,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":423,"modifier":"EXACT"},"end":{"value":441,"modifier":"EXACT"}},"description":"Low complexity","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":492,"modifier":"EXACT"},"end":{"value":517,"modifier":"EXACT"}},"description":"Acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Modified residue","location":{"start":{"value":511,"modifier":"EXACT"},"end":{"value":511,"modifier":"EXACT"}},"description":"Phosphoserine","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28197146"}]}],"keywords":[{"id":"KW-1039","category":"Cellular component","name":"Host endosome"},{"id":"KW-0597","category":"PTM","name":"Phosphoprotein"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0964","category":"Cellular component","name":"Secreted"},{"id":"KW-0946","category":"Cellular component","name":"Virion"},{"id":"KW-0920","category":"Cellular component","name":"Virion tegument"}],"references":[{"referenceNumber":1,"citation":{"id":"6087149","citationType":"journal article","authors":["Baer R.","Bankier A.T.","Biggin M.D.","Deininger P.L.","Farrell P.J.","Gibson T.J.","Hatfull G.","Hudson G.S.","Satchwell S.C.","Seguin C.","Tuffnell P.S.","Barrell B.G."],"citationCrossReferences":[{"database":"PubMed","id":"6087149"},{"database":"DOI","id":"10.1038/310207a0"}],"title":"DNA sequence and expression of the B95-8 Epstein-Barr virus genome.","publicationDate":"1984","journal":"Nature","firstPage":"207","lastPage":"211","volume":"310"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"]},{"referenceNumber":2,"citation":{"id":"12771413","citationType":"journal article","authors":["de Jesus O.","Smith P.R.","Spender L.C.","Elgueta Karstegl C.","Niller H.H.","Huang D.","Farrell P.J."],"citationCrossReferences":[{"database":"PubMed","id":"12771413"},{"database":"DOI","id":"10.1099/vir.0.19054-0"}],"title":"Updated Epstein-Barr virus (EBV) DNA sequence and analysis of a promoter for the BART (CST, BARF0) RNAs of EBV.","publicationDate":"2003","journal":"Virology","firstPage":"1443","lastPage":"1450","volume":"84"},"referencePositions":["GENOME REANNOTATION"]},{"referenceNumber":3,"citation":{"id":"15534216","citationType":"journal article","authors":["Johannsen E.","Luftig M.","Chase M.R.","Weicksel S.","Cahir-McFarland E.","Illanes D.","Sarracino D.","Kieff E."],"citationCrossReferences":[{"database":"PubMed","id":"15534216"},{"database":"DOI","id":"10.1073/pnas.0407320101"}],"title":"Proteins of purified Epstein-Barr virus.","publicationDate":"2004","journal":"Proc. Natl. Acad. Sci. U.S.A.","firstPage":"16286","lastPage":"16291","volume":"101"},"referencePositions":["SUBCELLULAR LOCATION"]},{"referenceNumber":4,"citation":{"id":"28197146","citationType":"journal article","authors":["Watanabe T.","Sakaida K.","Yoshida M.","Masud H.M.A.A.","Sato Y.","Goshima F.","Kimura H.","Murata T."],"citationCrossReferences":[{"database":"PubMed","id":"28197146"},{"database":"DOI","id":"10.3389/fmicb.2017.00125"}],"title":"The C-Terminus of Epstein-Barr Virus BRRF2 Is Required for its Proper Localization and Efficient Virus Production.","publicationDate":"2017","journal":"Front. Microbiol.","firstPage":"125","lastPage":"125","volume":"8"},"referencePositions":["SUBCELLULAR LOCATION","PHOSPHORYLATION AT SER-511"]},{"referenceNumber":5,"citation":{"id":"41073405","citationType":"journal article","authors":["Hu Z.L.","Li Z.Q.","Wang Y.","Luo Y.L.","Guo W.P.","Meng N.","Bu G.L.","Zhang L.L.","Li S.X.","Kong X.W.","Fang X.Y.","Wang Q.L.","Han R.K.","Zhao Z.","Zhao G.X.","Jiang Z.Y.","Jin R.X.","Zeng M.S.","Zhong Q."],"citationCrossReferences":[{"database":"PubMed","id":"41073405"},{"database":"DOI","id":"10.1038/s41467-025-64037-2"}],"title":"Extracellular vesicles derived EBV tegument protein BRRF2 suppresses cGAS phase separation to promote anti-viral innate immune evasion.","publicationDate":"2025","journal":"Nat. Commun.","firstPage":"9015","lastPage":"9015","volume":"16"},"referencePositions":["FUNCTION","SUBCELLULAR LOCATION","INTERACTION WITH HOST CGAS"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"V01555","properties":[{"key":"ProteinId","value":"CAA24815.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AJ507799","properties":[{"key":"ProteinId","value":"CAD53426.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"B43043","properties":[{"key":"EntryName","value":"QQBE30"}]},{"database":"RefSeq","id":"YP_401676.1","properties":[{"key":"NucleotideSequenceId","value":"NC_007605.1"}]},{"database":"AlphaFoldDB","id":"P03210","properties":[{"key":"Description","value":"-"}]},{"database":"iPTMnet","id":"P03210","properties":[{"key":"Description","value":"-"}]},{"database":"DNASU","id":"3783729","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"3783729","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"vg:3783729","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000153037","properties":[{"key":"Component","value":"Segment"}]},{"database":"GO","id":"GO:0005576","properties":[{"key":"GoTerm","value":"C:extracellular region"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0044174","properties":[{"key":"GoTerm","value":"C:host cell endosome"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"28197146"}]},{"database":"GO","id":"GO:0019033","properties":[{"key":"GoTerm","value":"C:viral tegument"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"41073405"}]},{"database":"GO","id":"GO:0141074","properties":[{"key":"GoTerm","value":"P:symbiont-mediated suppression of host cGAS-STING signal transduction"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"41073405"}]},{"database":"InterPro","id":"IPR008550","properties":[{"key":"EntryName","value":"Herpesvirus_BRRF2-like"}]},{"database":"Pfam","id":"PF05734","properties":[{"key":"EntryName","value":"DUF832"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MSGQQRGSVILVPEHLAGALTKLMSDFITGQDVTLSGGNIAVKIRDAINQTPGGGDVAILSSLFALWNALPTSGRQSSRDDLIPAAVQALTTAHNLCLGVIPGETSHKDTPESLLRAIVTGLQKLWVDSCGCPECLQCLKGLKAIKPGLYEIPRIIPHTKQCSPVNLLNMLVHKLVALRGHVQLAYDARVLTPDFHEIPDLDDSDAVFARTLLAALFHLNMFFILKDYITQDSMSLKQALSGHWMSATGNPLPAAPETLRDYLEAFRNSDNHFYLPTTGPLNTFQFPEELLGRVVVIDSSLCAASHVQDVITHGVGAGVPRPRFSALPPAPSREPQQTCSQLTSRGNESSRRNLGQPGGTSPAVPPVCPIVSLTASGAKQNRGGMGSLHLAKPEETSPAVSPVCPIASPAASRSKQHCGVTGSSQAAPSFSSVAPVASLSGDLEEEEEGSRESPSLPSSKKGDEEFEAWLEAQDANLEDVQREFSGLRVIGDEDEDGSEDGEFSDLDLSDSDHEGDEGGGAVGGGRSLHSLYSLSVV","length":537,"molWeight":56955,"crc64":"01059F793C6074E0","md5":"201DDA1D3C933FA54461100D9C7D55F2"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Region":4,"Compositional bias":3,"Modified residue":1},"uniParcId":"UPI000000CDDC"}}