{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P01050","secondaryAccessions":["P28501"],"uniProtkbId":"HIRV1_HIRME","entryAudit":{"firstPublicDate":"1986-07-21","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"1986-07-21","entryVersion":142,"sequenceVersion":1},"annotationScore":5.0,"organism":{"scientificName":"Hirudo medicinalis","commonName":"Medicinal leech","taxonId":6421,"lineage":["Eukaryota","Metazoa","Spiralia","Lophotrochozoa","Annelida","Clitellata","Hirudinea","Hirudinida","Hirudiniformes","Hirudinidae","Hirudo"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Hirudin variant-1"}},"alternativeNames":[{"fullName":{"value":"Hirudin-1"}},{"fullName":{"value":"Hirudin-I"}}],"innNames":[{"value":"Lepirudin"}]},"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17585879"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"36580989"}],"value":"Hirudin is a potent thrombin-specific protease inhibitor. It forms a stable non-covalent complex with alpha-thrombin, thereby abolishing its ability to cleave fibrinogen"}],"commentType":"FUNCTION"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"value":"Secreted","id":"SL-0243"}}]},{"texts":[{"value":"Available under the name Refludan (Hoechst Marion Roussel). Used to treat heparin-induced thrombocytopenia (HIT)"}],"commentType":"PHARMACEUTICAL"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the protease inhibitor I14 (hirudin) family"}],"commentType":"SIMILARITY"},{"commentType":"WEB RESOURCE","resourceName":"Refludan","resourceUrl":"https://www.rxlist.com/refludan-drug.htm","ftp":false,"note":"Clinical information on Refludan"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":65,"modifier":"EXACT"}},"description":"Hirudin variant-1","featureId":"PRO_0000195639"},{"type":"Region","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":3,"modifier":"EXACT"}},"description":"Interaction with thrombin active site"},{"type":"Region","location":{"start":{"value":39,"modifier":"EXACT"},"end":{"value":65,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":55,"modifier":"EXACT"},"end":{"value":65,"modifier":"EXACT"}},"description":"Interaction with fibrinogen-binding exosite of thrombin"},{"type":"Compositional bias","location":{"start":{"value":55,"modifier":"EXACT"},"end":{"value":65,"modifier":"EXACT"}},"description":"Acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Modified residue","location":{"start":{"value":63,"modifier":"EXACT"},"end":{"value":63,"modifier":"EXACT"}},"description":"Sulfotyrosine","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17685615"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"8251938"}]},{"type":"Glycosylation","location":{"start":{"value":45,"modifier":"EXACT"},"end":{"value":45,"modifier":"EXACT"}},"description":"O-linked (GalNAc...) threonine","evidences":[{"evidenceCode":"ECO:0000250"}],"featureId":""},{"type":"Disulfide bond","location":{"start":{"value":6,"modifier":"EXACT"},"end":{"value":14,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17585879"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"2JOO"}]},{"type":"Disulfide bond","location":{"start":{"value":16,"modifier":"EXACT"},"end":{"value":28,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17585879"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"2JOO"}]},{"type":"Disulfide bond","location":{"start":{"value":22,"modifier":"EXACT"},"end":{"value":39,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17585879"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"2JOO"}]},{"type":"Mutagenesis","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"Strongly decreased affinity for thrombin.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"1581311"}],"alternativeSequence":{"originalSequence":"V","alternativeSequences":["E","K"]}},{"type":"Mutagenesis","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"Decreased affinity for thrombin.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"1581311"}],"alternativeSequence":{"originalSequence":"V","alternativeSequences":["G","S"]}},{"type":"Mutagenesis","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"No effect.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"1581311"}],"alternativeSequence":{"originalSequence":"V","alternativeSequences":["L","R"]}},{"type":"Mutagenesis","location":{"start":{"value":2,"modifier":"EXACT"},"end":{"value":2,"modifier":"EXACT"}},"description":"Strongly decreased affinity for thrombin.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"1581311"}],"alternativeSequence":{"originalSequence":"V","alternativeSequences":["E","G"]}},{"type":"Mutagenesis","location":{"start":{"value":2,"modifier":"EXACT"},"end":{"value":2,"modifier":"EXACT"}},"description":"Decreased affinity for thrombin.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"1581311"}],"alternativeSequence":{"originalSequence":"V","alternativeSequences":["L"]}},{"type":"Mutagenesis","location":{"start":{"value":3,"modifier":"EXACT"},"end":{"value":3,"modifier":"EXACT"}},"description":"Strongly decreased affinity for thrombin.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"1581311"}],"alternativeSequence":{"originalSequence":"Y","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":4,"modifier":"EXACT"},"end":{"value":4,"modifier":"EXACT"}},"description":"Decreased affinity for thrombin.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"1581311"}],"alternativeSequence":{"originalSequence":"T","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":5,"modifier":"EXACT"},"end":{"value":5,"modifier":"EXACT"}},"description":"Decreased affinity for thrombin.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"1581311"}],"alternativeSequence":{"originalSequence":"D","alternativeSequences":["A","E"]}},{"type":"Mutagenesis","location":{"start":{"value":15,"modifier":"EXACT"},"end":{"value":15,"modifier":"EXACT"}},"description":"Strongly decreased affinity for thrombin.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"8135762"}],"alternativeSequence":{"originalSequence":"L","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":17,"modifier":"EXACT"},"end":{"value":17,"modifier":"EXACT"}},"description":"Slightly decreased affinity for thrombin.","alternativeSequence":{"originalSequence":"E","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":20,"modifier":"EXACT"},"end":{"value":20,"modifier":"EXACT"}},"description":"Decreased affinity for thrombin.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"8135762"}],"alternativeSequence":{"originalSequence":"N","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":21,"modifier":"EXACT"},"end":{"value":21,"modifier":"EXACT"}},"description":"Slightly decreased affinity for thrombin.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"8135762"}],"alternativeSequence":{"originalSequence":"V","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":56,"modifier":"EXACT"},"end":{"value":56,"modifier":"EXACT"}},"description":"Slightly decreased affinity for thrombin.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"1911777"}],"alternativeSequence":{"originalSequence":"F","alternativeSequences":["A","W"]}},{"type":"Mutagenesis","location":{"start":{"value":56,"modifier":"EXACT"},"end":{"value":56,"modifier":"EXACT"}},"description":"Strongly decreased affinity for thrombin.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"1911777"}],"alternativeSequence":{"originalSequence":"F","alternativeSequences":["I","L","T","V"]}},{"type":"Mutagenesis","location":{"start":{"value":56,"modifier":"EXACT"},"end":{"value":56,"modifier":"EXACT"}},"description":"No effect.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"1911777"}],"alternativeSequence":{"originalSequence":"F","alternativeSequences":["Y"]}},{"type":"Mutagenesis","location":{"start":{"value":60,"modifier":"EXACT"},"end":{"value":60,"modifier":"EXACT"}},"description":"Decreased affinity for thrombin.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"1911777"}],"alternativeSequence":{"originalSequence":"P","alternativeSequences":["A","G"]}},{"type":"Mutagenesis","location":{"start":{"value":63,"modifier":"EXACT"},"end":{"value":63,"modifier":"EXACT"}},"description":"Slightly decreased affinity for thrombin.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"1911777"}],"alternativeSequence":{"originalSequence":"Y","alternativeSequences":["A","E","L"]}},{"type":"Mutagenesis","location":{"start":{"value":63,"modifier":"EXACT"},"end":{"value":63,"modifier":"EXACT"}},"description":"No effect.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"1911777"}],"alternativeSequence":{"originalSequence":"Y","alternativeSequences":["F"]}},{"type":"Mutagenesis","location":{"start":{"value":63,"modifier":"EXACT"},"end":{"value":63,"modifier":"EXACT"}},"description":"Decreased affinity for thrombin.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"1911777"}],"alternativeSequence":{"originalSequence":"Y","alternativeSequences":["V"]}},{"type":"Beta strand","location":{"start":{"value":11,"modifier":"EXACT"},"end":{"value":17,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7A0D"}]},{"type":"Beta strand","location":{"start":{"value":20,"modifier":"EXACT"},"end":{"value":22,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7A0D"}]},{"type":"Beta strand","location":{"start":{"value":26,"modifier":"EXACT"},"end":{"value":29,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7A0D"}]},{"type":"Helix","location":{"start":{"value":30,"modifier":"EXACT"},"end":{"value":32,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7A0F"}]},{"type":"Helix","location":{"start":{"value":35,"modifier":"EXACT"},"end":{"value":37,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7A0D"}]},{"type":"Beta strand","location":{"start":{"value":38,"modifier":"EXACT"},"end":{"value":42,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7A0D"}]},{"type":"Helix","location":{"start":{"value":47,"modifier":"EXACT"},"end":{"value":52,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7A0D"}]},{"type":"Helix","location":{"start":{"value":56,"modifier":"EXACT"},"end":{"value":60,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2UUF"}]},{"type":"Helix","location":{"start":{"value":61,"modifier":"EXACT"},"end":{"value":63,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2UUF"}]}],"keywords":[{"id":"KW-0002","category":"Technical term","name":"3D-structure"},{"id":"KW-1203","category":"Molecular function","name":"Blood coagulation cascade inhibiting toxin"},{"id":"KW-0903","category":"Technical term","name":"Direct protein sequencing"},{"id":"KW-1015","category":"PTM","name":"Disulfide bond"},{"id":"KW-0325","category":"PTM","name":"Glycoprotein"},{"id":"KW-1199","category":"Molecular function","name":"Hemostasis impairing toxin"},{"id":"KW-0582","category":"Technical term","name":"Pharmaceutical"},{"id":"KW-0646","category":"Molecular function","name":"Protease inhibitor"},{"id":"KW-0964","category":"Cellular component","name":"Secreted"},{"id":"KW-0722","category":"Molecular function","name":"Serine protease inhibitor"},{"id":"KW-0765","category":"PTM","name":"Sulfation"},{"id":"KW-0800","category":"Molecular function","name":"Toxin"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-74KK49D6UQMDF","citationType":"journal article","authors":["Dodt J.","Mueller H.-P.","Seemueller U.","Chang J.-Y."],"title":"The complete amino acid sequence of hirudin, a thrombin specific inhibitor. Application of colour carboxymethylation.","publicationDate":"1984","journal":"FEBS Lett.","firstPage":"180","lastPage":"183","volume":"165"},"referencePositions":["PROTEIN SEQUENCE"]},{"referenceNumber":2,"citation":{"id":"CI-CM9LCPN3L2EGL","citationType":"book","authors":["Petersen T.E.","Roberts H.R.","Sottrup-Jensen L.","Magnusson S.","Bagdy D."],"publicationDate":"1976","bookName":"Protides of the biological fluids, Proc. 23th colloquium","editors":["Peeters H."],"firstPage":"145","lastPage":"149","publisher":"Pergamon Press","address":"New York"},"referencePositions":["PROTEIN SEQUENCE"]},{"referenceNumber":3,"citation":{"id":"2792365","citationType":"journal article","authors":["Scharf M.","Engels J.","Tripier D."],"citationCrossReferences":[{"database":"PubMed","id":"2792365"},{"database":"DOI","id":"10.1016/0014-5793(89)81070-1"}],"title":"Primary structures of new 'iso-hirudins'.","publicationDate":"1989","journal":"FEBS Lett.","firstPage":"105","lastPage":"110","volume":"255"},"referencePositions":["PROTEIN SEQUENCE OF 1-17 AND 28-65"]},{"referenceNumber":4,"citation":{"id":"1911777","citationType":"journal article","authors":["Betz A.","Hofsteenge J.","Stone S.R."],"citationCrossReferences":[{"database":"PubMed","id":"1911777"},{"database":"DOI","id":"10.1021/bi00105a006"}],"title":"Role of interactions involving C-terminal nonpolar residues of hirudin in the formation of the thrombin-hirudin complex.","publicationDate":"1991","journal":"Biochemistry","firstPage":"9848","lastPage":"9853","volume":"30"},"referencePositions":["MUTAGENESIS OF PHE-56; PRO-60 AND TYR-63"]},{"referenceNumber":5,"citation":{"id":"1581311","citationType":"journal article","authors":["Betz A.","Hofsteenge J.","Stone S.R."],"citationCrossReferences":[{"database":"PubMed","id":"1581311"},{"database":"DOI","id":"10.1021/bi00134a004"}],"title":"Interaction of the N-terminal region of hirudin with the active-site cleft of thrombin.","publicationDate":"1992","journal":"Biochemistry","firstPage":"4557","lastPage":"4562","volume":"31"},"referencePositions":["MUTAGENESIS OF VAL-1; VAL-2; TYR-3; THR-4 AND ASP-5"]},{"referenceNumber":6,"citation":{"id":"8135762","citationType":"journal article","authors":["Betz A.","Hopkins P.C.R.","Le Bonniec B.F.","Stone S.R."],"citationCrossReferences":[{"database":"PubMed","id":"8135762"},{"database":"DOI","id":"10.1042/bj2980507"}],"title":"Contribution of interactions with the core domain of hirudin to the stability of its complex with thrombin.","publicationDate":"1994","journal":"Biochem. J.","firstPage":"507","lastPage":"510","volume":"298"},"referencePositions":["MUTAGENESIS OF LEU-15; ASN-20 AND VAL-21"]},{"referenceNumber":7,"citation":{"id":"36580989","citationType":"journal article","authors":["Mo Z.","Xiao Z.","He C."],"citationCrossReferences":[{"database":"PubMed","id":"36580989"},{"database":"DOI","id":"10.1016/j.biochi.2022.12.013"}],"title":"Functional expression of a thrombin exosite I inhibitor triabin in Escherichia coli and elucidation of the role of key residues in its inhibitory activity.","publicationDate":"2023","journal":"Biochimie","firstPage":"13","lastPage":"19","volume":"208"},"referencePositions":["FUNCTION"]},{"referenceNumber":8,"citation":{"id":"2567183","citationType":"journal article","authors":["Folkers P.J.M.","Clore G.M.","Driscoll P.C.","Dodt J.","Koehler S.","Gronenborn A.M."],"citationCrossReferences":[{"database":"PubMed","id":"2567183"},{"database":"DOI","id":"10.1021/bi00432a038"}],"title":"Solution structure of recombinant hirudin and the Lys-47-->Glu mutant: a nuclear magnetic resonance and hybrid distance geometry-dynamical simulated annealing study.","publicationDate":"1989","journal":"Biochemistry","firstPage":"2601","lastPage":"2617","volume":"28"},"referencePositions":["STRUCTURE BY NMR","DISULFIDE BONDS"]},{"referenceNumber":9,"citation":{"id":"2765488","citationType":"journal article","authors":["Haruyama H.","Wuethrich K."],"citationCrossReferences":[{"database":"PubMed","id":"2765488"},{"database":"DOI","id":"10.1021/bi00436a027"}],"title":"Conformation of recombinant desulfatohirudin in aqueous solution determined by nuclear magnetic resonance.","publicationDate":"1989","journal":"Biochemistry","firstPage":"4301","lastPage":"4312","volume":"28"},"referencePositions":["STRUCTURE BY NMR"]},{"referenceNumber":10,"citation":{"id":"2369893","citationType":"journal article","authors":["Gruetter M.G.","Priestle J.P.","Rahuel J.","Grossenbacher H.","Bode W.","Hofsteenge J.","Stone S.R."],"citationCrossReferences":[{"database":"PubMed","id":"2369893"},{"database":"DOI","id":"10.1002/j.1460-2075.1990.tb07410.x"}],"title":"Crystal structure of the thrombin-hirudin complex: a novel mode of serine protease inhibition.","publicationDate":"1990","journal":"EMBO J.","firstPage":"2361","lastPage":"2365","volume":"9"},"referencePositions":["X-RAY CRYSTALLOGRAPHY (2.95 ANGSTROMS) IN COMPLEX WITH THROMBIN"]},{"referenceNumber":11,"citation":{"id":"1517214","citationType":"journal article","authors":["Vitali J.","Martin P.D.","Malkowski M.G.","Robertson W.D.","Lazar J.B.","Winant R.C.","Johnson P.H.","Edwards B.F.P."],"citationCrossReferences":[{"database":"PubMed","id":"1517214"},{"database":"DOI","id":"10.1016/s0021-9258(19)37095-4"}],"title":"The structure of a complex of bovine alpha-thrombin and recombinant hirudin at 2.8-A resolution.","publicationDate":"1992","journal":"J. Biol. Chem.","firstPage":"17670","lastPage":"17678","volume":"267"},"referencePositions":["X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 51-65 IN COMPLEX WITH THROMBIN"]},{"referenceNumber":12,"citation":{"id":"1335515","citationType":"journal article","authors":["Szyperski T.","Guentert P.","Stone S.R.","Wuethrich K."],"citationCrossReferences":[{"database":"PubMed","id":"1335515"},{"database":"DOI","id":"10.1016/0022-2836(92)90325-e"}],"title":"Nuclear magnetic resonance solution structure of hirudin(1-51) and comparison with corresponding three-dimensional structures determined using the complete 65-residue hirudin polypeptide chain.","publicationDate":"1992","journal":"J. Mol. Biol.","firstPage":"1193","lastPage":"1205","volume":"228"},"referencePositions":["STRUCTURE BY NMR OF 1-51"]},{"referenceNumber":13,"citation":{"id":"8251938","citationType":"journal article","authors":["Priestle J.P.","Rahuel J.","Rink H.","Tones M.","Gruetter M.G."],"citationCrossReferences":[{"database":"PubMed","id":"8251938"},{"database":"DOI","id":"10.1002/pro.5560021009"}],"title":"Changes in interactions in complexes of hirudin derivatives and human alpha-thrombin due to different crystal forms.","publicationDate":"1993","journal":"Protein Sci.","firstPage":"1630","lastPage":"1642","volume":"2"},"referencePositions":["X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 54-65 IN COMPLEX WITH THROMBIN","SULFATION AT TYR-63"]},{"referenceNumber":14,"citation":{"id":"17585879","citationType":"journal article","authors":["Song X.","Mo W.","Liu X.","Zhu L.","Yan X.","Song H.","Dai L."],"citationCrossReferences":[{"database":"PubMed","id":"17585879"},{"database":"DOI","id":"10.1016/j.bbrc.2007.06.014"}],"title":"The NMR solution structure of recombinant RGD-hirudin.","publicationDate":"2007","journal":"Biochem. Biophys. Res. Commun.","firstPage":"103","lastPage":"108","volume":"360"},"referencePositions":["STRUCTURE BY NMR OF 1-57 OF MUTANT 32-R--D-34","FUNCTION","DISULFIDE BONDS"]},{"referenceNumber":15,"citation":{"id":"17685615","citationType":"journal article","authors":["Liu C.C.","Brustad E.","Liu W.","Schultz P.G."],"citationCrossReferences":[{"database":"PubMed","id":"17685615"},{"database":"DOI","id":"10.1021/ja0735002"}],"title":"Crystal structure of a biosynthetic sulfo-hirudin complexed to thrombin.","publicationDate":"2007","journal":"J. Am. Chem. Soc.","firstPage":"10648","lastPage":"10649","volume":"129"},"referencePositions":["X-RAY CRYSTALLOGRAPHY (1.84 ANGSTROMS)","DISULFIDE BONDS","SULFATION AT TYR-63"]}],"uniProtKBCrossReferences":[{"database":"PIR","id":"A91318","properties":[{"key":"EntryName","value":"HULXH"}]},{"database":"PIR","id":"S05672","properties":[{"key":"EntryName","value":"S05672"}]},{"database":"PDB","id":"1AD8","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.00 A"},{"key":"Chains","value":"I=55-64"}]},{"database":"PDB","id":"1AE8","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.00 A"},{"key":"Chains","value":"I=55-64"}]},{"database":"PDB","id":"1AFE","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.00 A"},{"key":"Chains","value":"I=55-64"}]},{"database":"PDB","id":"1AHT","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.60 A"},{"key":"Chains","value":"I=55-64"}]},{"database":"PDB","id":"1AI8","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.85 A"},{"key":"Chains","value":"I=53-64"}]},{"database":"PDB","id":"1AWF","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.20 A"},{"key":"Chains","value":"I=55-64"}]},{"database":"PDB","id":"1AY6","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.80 A"},{"key":"Chains","value":"I=55-64"}]},{"database":"PDB","id":"1BA8","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.80 A"},{"key":"Chains","value":"C=53-64"}]},{"database":"PDB","id":"1BB0","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.10 A"},{"key":"Chains","value":"C=53-64"}]},{"database":"PDB","id":"1BCU","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.00 A"},{"key":"Chains","value":"I=54-65"}]},{"database":"PDB","id":"1CA8","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.10 A"},{"key":"Chains","value":"C=53-64"}]},{"database":"PDB","id":"1D3D","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.04 A"},{"key":"Chains","value":"H=54-65"}]},{"database":"PDB","id":"1D3P","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.10 A"},{"key":"Chains","value":"H=54-65"}]},{"database":"PDB","id":"1D3Q","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.90 A"},{"key":"Chains","value":"H=54-65"}]},{"database":"PDB","id":"1D3T","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"3.00 A"},{"key":"Chains","value":"H=54-65"}]},{"database":"PDB","id":"1D4P","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.07 A"},{"key":"Chains","value":"H=54-65"}]},{"database":"PDB","id":"1H8D","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.40 A"},{"key":"Chains","value":"I=55-64"}]},{"database":"PDB","id":"1H8I","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.75 A"},{"key":"Chains","value":"I=55-64"}]},{"database":"PDB","id":"1HIC","properties":[{"key":"Method","value":"NMR"},{"key":"Resolution","value":"-"},{"key":"Chains","value":"A=1-51"}]},{"database":"PDB","id":"1HRT","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.80 A"},{"key":"Chains","value":"I=1-65"}]},{"database":"PDB","id":"1HXE","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.10 A"},{"key":"Chains","value":"I=55-64"}]},{"database":"PDB","id":"1HXF","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.10 A"},{"key":"Chains","value":"I=55-64"}]},{"database":"PDB","id":"1NO9","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.90 A"},{"key":"Chains","value":"I=55-65"}]},{"database":"PDB","id":"1QHR","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.20 A"},{"key":"Chains","value":"I=55-64"}]},{"database":"PDB","id":"1QJ1","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.00 A"},{"key":"Chains","value":"I=55-64"}]},{"database":"PDB","id":"1QJ6","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.20 A"},{"key":"Chains","value":"I=55-64"}]},{"database":"PDB","id":"1QJ7","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.20 A"},{"key":"Chains","value":"I=55-64"}]},{"database":"PDB","id":"1TMT","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.20 A"},{"key":"Chains","value":"I=53-65"}]},{"database":"PDB","id":"1TMU","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.50 A"},{"key":"Chains","value":"I=55-65"}]},{"database":"PDB","id":"1UMA","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.00 A"},{"key":"Chains","value":"I=55-64"}]},{"database":"PDB","id":"1WBG","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.20 A"},{"key":"Chains","value":"I=55-64"}]},{"database":"PDB","id":"2HIR","properties":[{"key":"Method","value":"NMR"},{"key":"Resolution","value":"-"},{"key":"Chains","value":"A=1-65"}]},{"database":"PDB","id":"2JOO","properties":[{"key":"Method","value":"NMR"},{"key":"Resolution","value":"-"},{"key":"Chains","value":"A=1-65"}]},{"database":"PDB","id":"2PW8","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.84 A"},{"key":"Chains","value":"I=3-65"}]},{"database":"PDB","id":"2UUF","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.26 A"},{"key":"Chains","value":"H=55-64"}]},{"database":"PDB","id":"2UUJ","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.32 A"},{"key":"Chains","value":"H=55-64"}]},{"database":"PDB","id":"2UUK","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.39 A"},{"key":"Chains","value":"H=55-64"}]},{"database":"PDB","id":"2V3O","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.79 A"},{"key":"Chains","value":"I=56-65"}]},{"database":"PDB","id":"2ZC9","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.58 A"},{"key":"Chains","value":"I=54-64"}]},{"database":"PDB","id":"2ZDA","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.73 A"},{"key":"Chains","value":"I=54-64"}]},{"database":"PDB","id":"2ZDV","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.72 A"},{"key":"Chains","value":"I=54-64"}]},{"database":"PDB","id":"2ZF0","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.20 A"},{"key":"Chains","value":"I=54-64"}]},{"database":"PDB","id":"2ZFF","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.47 A"},{"key":"Chains","value":"I=54-64"}]},{"database":"PDB","id":"2ZFP","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.25 A"},{"key":"Chains","value":"I=54-64"}]},{"database":"PDB","id":"2ZGB","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.60 A"},{"key":"Chains","value":"I=54-64"}]},{"database":"PDB","id":"2ZGX","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.80 A"},{"key":"Chains","value":"I=54-64"}]},{"database":"PDB","id":"2ZHQ","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.96 A"},{"key":"Chains","value":"I=54-64"}]},{"database":"PDB","id":"2ZI2","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.65 A"},{"key":"Chains","value":"I=54-64"}]},{"database":"PDB","id":"2ZIQ","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.65 A"},{"key":"Chains","value":"I=54-64"}]},{"database":"PDB","id":"2ZNK","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.80 A"},{"key":"Chains","value":"I=54-64"}]},{"database":"PDB","id":"2ZO3","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.70 A"},{"key":"Chains","value":"I=54-64"}]},{"database":"PDB","id":"3D49","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.50 A"},{"key":"Chains","value":"I=54-64"}]},{"database":"PDB","id":"3DHK","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.73 A"},{"key":"Chains","value":"I=54-64"}]},{"database":"PDB","id":"3DT0","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.40 A"},{"key":"Chains","value":"I=54-64"}]},{"database":"PDB","id":"3DUX","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.60 A"},{"key":"Chains","value":"I=54-64"}]},{"database":"PDB","id":"3EGK","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.20 A"},{"key":"Chains","value":"I=54-64"}]},{"database":"PDB","id":"3EQ0","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.53 A"},{"key":"Chains","value":"I=54-64"}]},{"database":"PDB","id":"3LDX","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.25 A"},{"key":"Chains","value":"I=55-65"}]},{"database":"PDB","id":"3UTU","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.55 A"},{"key":"Chains","value":"I=54-64"}]},{"database":"PDB","id":"4AX9","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.90 A"},{"key":"Chains","value":"I=55-65"}]},{"database":"PDB","id":"4BAH","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.94 A"},{"key":"Chains","value":"D=53-64"}]},{"database":"PDB","id":"4BAK","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.94 A"},{"key":"Chains","value":"D=53-64"}]},{"database":"PDB","id":"4BAM","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.88 A"},{"key":"Chains","value":"D=53-64"}]},{"database":"PDB","id":"4BAN","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.87 A"},{"key":"Chains","value":"D=53-64"}]},{"database":"PDB","id":"4BAQ","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.89 A"},{"key":"Chains","value":"D=53-64"}]},{"database":"PDB","id":"4HIR","properties":[{"key":"Method","value":"NMR"},{"key":"Resolution","value":"-"},{"key":"Chains","value":"A=1-65"}]},{"database":"PDB","id":"4LXB","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.61 A"},{"key":"Chains","value":"I=53-65"}]},{"database":"PDB","id":"4MLF","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.20 A"},{"key":"Chains","value":"D=1-65"}]},{"database":"PDB","id":"4YES","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.50 A"},{"key":"Chains","value":"H=54-65"}]},{"database":"PDB","id":"5HIR","properties":[{"key":"Method","value":"NMR"},{"key":"Resolution","value":"-"},{"key":"Chains","value":"A=1-65"}]},{"database":"PDB","id":"6HIR","properties":[{"key":"Method","value":"NMR"},{"key":"Resolution","value":"-"},{"key":"Chains","value":"A=1-65"}]},{"database":"PDB","id":"7A0D","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.60 A"},{"key":"Chains","value":"III=1-65"}]},{"database":"PDB","id":"7A0E","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.90 A"},{"key":"Chains","value":"III=1-65"}]},{"database":"PDB","id":"7A0F","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.70 A"},{"key":"Chains","value":"III=1-65"}]},{"database":"PDBsum","id":"1AD8","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"1AE8","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"1AFE","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"1AHT","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"1AI8","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"1AWF","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"1AY6","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"1BA8","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"1BB0","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"1BCU","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"1CA8","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"1D3D","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"1D3P","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"1D3Q","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"1D3T","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"1D4P","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"1H8D","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"1H8I","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"1HIC","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"1HRT","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"1HXE","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"1HXF","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"1NO9","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"1QHR","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"1QJ1","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"1QJ6","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"1QJ7","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"1TMT","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"1TMU","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"1UMA","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"1WBG","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"2HIR","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"2JOO","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"2PW8","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"2UUF","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"2UUJ","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"2UUK","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"2V3O","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"2ZC9","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"2ZDA","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"2ZDV","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"2ZF0","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"2ZFF","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"2ZFP","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"2ZGB","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"2ZGX","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"2ZHQ","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"2ZI2","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"2ZIQ","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"2ZNK","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"2ZO3","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"3D49","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"3DHK","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"3DT0","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"3DUX","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"3EGK","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"3EQ0","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"3LDX","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"3UTU","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"4AX9","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"4BAH","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"4BAK","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"4BAM","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"4BAN","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"4BAQ","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"4HIR","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"4LXB","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"4MLF","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"4YES","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"5HIR","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"6HIR","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7A0D","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7A0E","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7A0F","properties":[{"key":"Description","value":"-"}]},{"database":"AlphaFoldDB","id":"P01050","properties":[{"key":"Description","value":"-"}]},{"database":"BMRB","id":"P01050","properties":[{"key":"Description","value":"-"}]},{"database":"MDposit","id":"P01050","properties":[{"key":"Description","value":"-"}]},{"database":"MDRepo","id":"P01050","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"P01050","properties":[{"key":"Description","value":"-"}]},{"database":"Allergome","id":"9843","properties":[{"key":"AllergenName","value":"Hir me Hirudin"}]},{"database":"MEROPS","id":"I14.001","properties":[{"key":"Description","value":"-"}]},{"database":"EvolutionaryTrace","id":"P01050","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005576","properties":[{"key":"GoTerm","value":"C:extracellular region"},{"key":"GoEvidenceType","value":"IDA:CAFA"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"3013692"}]},{"database":"GO","id":"GO:0004867","properties":[{"key":"GoTerm","value":"F:serine-type endopeptidase inhibitor activity"},{"key":"GoEvidenceType","value":"IDA:CAFA"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"3013692"}]},{"database":"DisProt","id":"DP00137","properties":[{"key":"Description","value":"-"}]},{"database":"FunFam","id":"2.70.10.10:FF:000001","properties":[{"key":"EntryName","value":"Hirudin variant-1"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.70.10.10","properties":[{"key":"EntryName","value":"Thrombin Inhibitor (Hirudin), subunit I"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR024793","properties":[{"key":"EntryName","value":"Hirudin"}]},{"database":"InterPro","id":"IPR011061","properties":[{"key":"EntryName","value":"Hirudin/antistatin"}]},{"database":"InterPro","id":"IPR000429","properties":[{"key":"EntryName","value":"Prot_inh_hirudin"}]},{"database":"Pfam","id":"PF00713","properties":[{"key":"EntryName","value":"Hirudin"},{"key":"MatchStatus","value":"1"}]},{"database":"PIRSF","id":"PIRSF001640","properties":[{"key":"EntryName","value":"Hirudin"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR00777","properties":[{"key":"EntryName","value":"HIRUDIN"}]},{"database":"SUPFAM","id":"SSF57262","properties":[{"key":"EntryName","value":"Leech antihemostatic proteins"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"VVYTDCTESGQNLCLCEGSNVCGQGNKCILGSDGEKNQCVTGEGTPKPQSHNDGDFEEIPEEYLQ","length":65,"molWeight":6970,"crc64":"9085A5876E3DE9FF","md5":"1DCC73846393360B7E6750541F884933"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBCELLULAR LOCATION":1,"PHARMACEUTICAL":1,"SIMILARITY":1,"WEB RESOURCE":1},"countByFeatureType":{"Chain":1,"Region":3,"Compositional bias":1,"Modified residue":1,"Glycosylation":1,"Disulfide bond":3,"Mutagenesis":19,"Beta strand":4,"Helix":5},"uniParcId":"UPI000002BA98"}}