{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P00887","secondaryAccessions":["P78301"],"uniProtkbId":"AROH_ECOLI","entryAudit":{"firstPublicDate":"1986-07-21","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2003-06-20","entryVersion":177,"sequenceVersion":4},"annotationScore":4.0,"organism":{"scientificName":"Escherichia coli (strain K12)","taxonId":83333,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Enterobacterales","Enterobacteriaceae","Escherichia"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Phospho-2-dehydro-3-deoxyheptonate aldolase, Trp-sensitive"},"ecNumbers":[{"value":"2.5.1.54"}]},"alternativeNames":[{"fullName":{"value":"3-deoxy-D-arabino-heptulosonate 7-phosphate synthase"}},{"fullName":{"value":"DAHP synthase"}},{"fullName":{"value":"Phospho-2-keto-3-deoxyheptonate aldolase"}}]},"genes":[{"geneName":{"value":"aroH"},"orderedLocusNames":[{"value":"b1704"},{"value":"JW1694"}]}],"comments":[{"texts":[{"value":"Stereospecific condensation of phosphoenolpyruvate (PEP) and D-erythrose-4-phosphate (E4P) giving rise to 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP)"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"D-erythrose 4-phosphate + phosphoenolpyruvate + H2O = 7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate + phosphate","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:14717"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:16897"},{"database":"ChEBI","id":"CHEBI:43474"},{"database":"ChEBI","id":"CHEBI:58394"},{"database":"ChEBI","id":"CHEBI:58702"}],"ecNumber":"2.5.1.54"}},{"texts":[{"value":"Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 1/7"}],"commentType":"PATHWAY"},{"commentType":"INTERACTION","interactions":[{"interactantOne":{"uniProtKBAccession":"P00887","intActId":"EBI-1125143"},"interactantTwo":{"uniProtKBAccession":"P0A6F5","geneName":"groEL","intActId":"EBI-543750"},"numberOfExperiments":3,"organismDiffer":false}]},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01406"}],"value":"Cytoplasm","id":"SL-0086"}}]},{"texts":[{"value":"There are 3 DAHP synthases, AroH is feedback-inhibited by Trp. The other 2 DAHP synthases are Tyr- and Phe-sensitive, respectively"}],"commentType":"MISCELLANEOUS"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01406"}],"value":"Belongs to the class-I DAHP synthase family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":348,"modifier":"EXACT"}},"description":"Phospho-2-dehydro-3-deoxyheptonate aldolase, Trp-sensitive","featureId":"PRO_0000140840"},{"type":"Domain","location":{"start":{"value":50,"modifier":"EXACT"},"end":{"value":348,"modifier":"EXACT"}},"description":"DAHPS class-I TIM-barrel","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01406"}]},{"type":"Binding site","location":{"start":{"value":91,"modifier":"EXACT"},"end":{"value":91,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58702"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01406"}],"ligand":{"name":"phosphoenolpyruvate","id":"ChEBI:CHEBI:58702"}},{"type":"Binding site","location":{"start":{"value":96,"modifier":"EXACT"},"end":{"value":96,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58702"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01406"}],"ligand":{"name":"phosphoenolpyruvate","id":"ChEBI:CHEBI:58702"}},{"type":"Binding site","location":{"start":{"value":163,"modifier":"EXACT"},"end":{"value":163,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58702"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01406"}],"ligand":{"name":"phosphoenolpyruvate","id":"ChEBI:CHEBI:58702"}},{"type":"Binding site","location":{"start":{"value":164,"modifier":"EXACT"},"end":{"value":164,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58702"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01406"}],"ligand":{"name":"phosphoenolpyruvate","id":"ChEBI:CHEBI:58702"}},{"type":"Binding site","location":{"start":{"value":185,"modifier":"EXACT"},"end":{"value":185,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58702"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01406"}],"ligand":{"name":"phosphoenolpyruvate","id":"ChEBI:CHEBI:58702"}},{"type":"Binding site","location":{"start":{"value":233,"modifier":"EXACT"},"end":{"value":233,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58702"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01406"}],"ligand":{"name":"phosphoenolpyruvate","id":"ChEBI:CHEBI:58702"}},{"type":"Binding site","location":{"start":{"value":267,"modifier":"EXACT"},"end":{"value":267,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58702"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01406"}],"ligand":{"name":"phosphoenolpyruvate","id":"ChEBI:CHEBI:58702"}},{"type":"Sequence conflict","location":{"start":{"value":203,"modifier":"EXACT"},"end":{"value":205,"modifier":"EXACT"}},"description":"in Ref. 2; AAA23493","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"RAS","alternativeSequences":["AQ"]}},{"type":"Sequence conflict","location":{"start":{"value":333,"modifier":"EXACT"},"end":{"value":334,"modifier":"EXACT"}},"description":"in Ref. 2; AAA23493 and 6; CAA27956","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"TE","alternativeSequences":["RQ"]}},{"type":"Sequence conflict","location":{"start":{"value":333,"modifier":"EXACT"},"end":{"value":333,"modifier":"EXACT"}},"description":"in Ref. 1; AAA23497","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"T","alternativeSequences":["S"]}}],"keywords":[{"id":"KW-0028","category":"Biological process","name":"Amino-acid biosynthesis"},{"id":"KW-0057","category":"Biological process","name":"Aromatic amino acid biosynthesis"},{"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0808","category":"Molecular function","name":"Transferase"}],"references":[{"referenceNumber":1,"citation":{"id":"1677907","citationType":"journal article","authors":["Hudson G.S.","Rellos P.","Davidson B.E."],"citationCrossReferences":[{"database":"PubMed","id":"1677907"},{"database":"DOI","id":"10.1016/0378-1119(91)90544-l"}],"title":"Two promoters control the aroH gene of Escherichia coli.","publicationDate":"1991","journal":"Gene","firstPage":"87","lastPage":"91","volume":"102"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"]},{"referenceNumber":2,"citation":{"id":"2903857","citationType":"journal article","authors":["Ray J.M.","Yanofsky C.","Bauerle R."],"citationCrossReferences":[{"database":"PubMed","id":"2903857"},{"database":"DOI","id":"10.1128/jb.170.12.5500-5506.1988"}],"title":"Mutational analysis of the catalytic and feedback sites of the tryptophan-sensitive 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase of Escherichia coli.","publicationDate":"1988","journal":"J. Bacteriol.","firstPage":"5500","lastPage":"5506","volume":"170"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"]},{"referenceNumber":3,"citation":{"id":"9097039","citationType":"journal article","authors":["Aiba H.","Baba T.","Fujita K.","Hayashi K.","Inada T.","Isono K.","Itoh T.","Kasai H.","Kashimoto K.","Kimura S.","Kitakawa M.","Kitagawa M.","Makino K.","Miki T.","Mizobuchi K.","Mori H.","Mori T.","Motomura K.","Nakade S.","Nakamura Y.","Nashimoto H.","Nishio Y.","Oshima T.","Saito N.","Sampei G.","Seki Y.","Sivasundaram S.","Tagami H.","Takeda J.","Takemoto K.","Takeuchi Y.","Wada C.","Yamamoto Y.","Horiuchi T."],"citationCrossReferences":[{"database":"PubMed","id":"9097039"},{"database":"DOI","id":"10.1093/dnares/3.6.363"}],"title":"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map.","publicationDate":"1996","journal":"DNA Res.","firstPage":"363","lastPage":"377","volume":"3"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / W3110 / ATCC 27325 / DSM 5911","type":"STRAIN"}]},{"referenceNumber":4,"citation":{"id":"9278503","citationType":"journal article","authors":["Blattner F.R.","Plunkett G. III","Bloch C.A.","Perna N.T.","Burland V.","Riley M.","Collado-Vides J.","Glasner J.D.","Rode C.K.","Mayhew G.F.","Gregor J.","Davis N.W.","Kirkpatrick H.A.","Goeden M.A.","Rose D.J.","Mau B.","Shao Y."],"citationCrossReferences":[{"database":"PubMed","id":"9278503"},{"database":"DOI","id":"10.1126/science.277.5331.1453"}],"title":"The complete genome sequence of Escherichia coli K-12.","publicationDate":"1997","journal":"Science","firstPage":"1453","lastPage":"1462","volume":"277"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / MG1655 / ATCC 47076","type":"STRAIN"}]},{"referenceNumber":5,"citation":{"id":"16738553","citationType":"journal article","authors":["Hayashi K.","Morooka N.","Yamamoto Y.","Fujita K.","Isono K.","Choi S.","Ohtsubo E.","Baba T.","Wanner B.L.","Mori H.","Horiuchi T."],"citationCrossReferences":[{"database":"PubMed","id":"16738553"},{"database":"DOI","id":"10.1038/msb4100049"}],"title":"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.","publicationDate":"2006","journal":"Mol. Syst. Biol.","firstPage":"E1","lastPage":"E5","volume":"2"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / W3110 / ATCC 27325 / DSM 5911","type":"STRAIN"}]},{"referenceNumber":6,"citation":{"id":"6167722","citationType":"journal article","authors":["Zurawski G.","Gunsalus R.P.","Brown K.D.","Yanofsky C."],"citationCrossReferences":[{"database":"PubMed","id":"6167722"},{"database":"DOI","id":"10.1016/0022-2836(81)90334-x"}],"title":"Structure and regulation of aroH, the structural gene for the tryptophan-repressible 3-deoxy-D-arabino-heptulosonic acid-7-phosphate synthetase of Escherichia coli.","publicationDate":"1981","journal":"J. Mol. Biol.","firstPage":"47","lastPage":"73","volume":"145"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-36 AND 232-348"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"M38266","properties":[{"key":"ProteinId","value":"AAA23497.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"J04221","properties":[{"key":"ProteinId","value":"AAA23493.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"U00096","properties":[{"key":"ProteinId","value":"AAC74774.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AP009048","properties":[{"key":"ProteinId","value":"BAA15473.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"V00261","properties":[{"key":"ProteinId","value":"CAA23510.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"X04373","properties":[{"key":"ProteinId","value":"CAA27956.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"H64928","properties":[{"key":"EntryName","value":"ADECH"}]},{"database":"RefSeq","id":"NP_416219.1","properties":[{"key":"NucleotideSequenceId","value":"NC_000913.3"}]},{"database":"RefSeq","id":"WP_001082229.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_CP009789.1"}]},{"database":"AlphaFoldDB","id":"P00887","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"P00887","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"4260296","properties":[{"key":"Interactions","value":"16"}]},{"database":"BioGRID","id":"850589","properties":[{"key":"Interactions","value":"1"}]},{"database":"DIP","id":"DIP-6846N","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"P00887","properties":[{"key":"Number of interactors","value":"325"}]},{"database":"IntAct","id":"P00887","properties":[{"key":"Interactions","value":"5"}]},{"database":"STRING","id":"511145.b1704","properties":[{"key":"Description","value":"-"}]},{"database":"jPOST","id":"P00887","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"511145-b1704","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblBacteria","id":"AAC74774","properties":[{"key":"ProteinId","value":"AAC74774"},{"key":"GeneId","value":"b1704"}]},{"database":"GeneID","id":"946229","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ecj:JW1694","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"eco:b1704","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ecoc:C3026_09760","properties":[{"key":"Description","value":"-"}]},{"database":"PATRIC","id":"fig|1411691.4.peg.553","properties":[{"key":"GeneDesignation","value":"-"}]},{"database":"EchoBASE","id":"EB0078","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"COG0722","properties":[{"key":"ToxonomicScope","value":"Bacteria"}]},{"database":"HOGENOM","id":"CLU_030903_0_1_6","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"P00887","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"PCLSWED","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"9807331at2","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"P00887","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"EcoCyc:AROH-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"MetaCyc:AROH-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"UniPathway","id":"UPA00053","properties":[{"key":"RectionId","value":"UER00084"}]},{"database":"PRO","id":"PR:P00887","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000000625","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"AbasyAtlas","id":"P00887","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"IDA:EcoliWiki"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"5335890"}]},{"database":"GO","id":"GO:0003849","properties":[{"key":"GoTerm","value":"F:3-deoxy-7-phosphoheptulonate synthase activity"},{"key":"GoEvidenceType","value":"IDA:EcoliWiki"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"5335890"}]},{"database":"GO","id":"GO:0042802","properties":[{"key":"GoTerm","value":"F:identical protein binding"},{"key":"GoEvidenceType","value":"IDA:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"1672127"}]},{"database":"GO","id":"GO:0009073","properties":[{"key":"GoTerm","value":"P:aromatic amino acid family biosynthetic process"},{"key":"GoEvidenceType","value":"IDA:EcoliWiki"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"5335890"}]},{"database":"GO","id":"GO:0009423","properties":[{"key":"GoTerm","value":"P:chorismate biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniPathway"}]},{"database":"FunFam","id":"3.20.20.70:FF:000005","properties":[{"key":"EntryName","value":"Phospho-2-dehydro-3-deoxyheptonate aldolase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.20.20.70","properties":[{"key":"EntryName","value":"Aldolase class I"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR013785","properties":[{"key":"EntryName","value":"Aldolase_TIM"}]},{"database":"InterPro","id":"IPR006218","properties":[{"key":"EntryName","value":"DAHP1/KDSA"}]},{"database":"InterPro","id":"IPR006219","properties":[{"key":"EntryName","value":"DAHP_synth_1"}]},{"database":"NCBIfam","id":"TIGR00034","properties":[{"key":"EntryName","value":"aroFGH"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"NF009395","properties":[{"key":"EntryName","value":"PRK12755.1"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"NF009396","properties":[{"key":"EntryName","value":"PRK12756.1"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR21225","properties":[{"key":"EntryName","value":"PHOSPHO-2-DEHYDRO-3-DEOXYHEPTONATE ALDOLASE DAHP SYNTHETASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR21225:SF6","properties":[{"key":"EntryName","value":"PHOSPHO-2-DEHYDRO-3-DEOXYHEPTONATE ALDOLASE, TRP-SENSITIVE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00793","properties":[{"key":"EntryName","value":"DAHP_synth_1"},{"key":"MatchStatus","value":"1"}]},{"database":"PIRSF","id":"PIRSF001361","properties":[{"key":"EntryName","value":"DAHP_synthase"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF51569","properties":[{"key":"EntryName","value":"Aldolase"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS52061","properties":[{"key":"EntryName","value":"DAHPS_I"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEKILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVGWKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGAIGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMTIYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRRQLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGSQPLTYGQSITDPCLGWEDTERLVEKLASAVDTRF","length":348,"molWeight":38735,"crc64":"52C3C482447226E0","md5":"166E04931AD678BCD8588B541710F9C1"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"PATHWAY":1,"INTERACTION":1,"SUBCELLULAR LOCATION":1,"MISCELLANEOUS":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Domain":1,"Binding site":7,"Sequence conflict":3},"uniParcId":"UPI00001680CA"}}