{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P00099","uniProtkbId":"CY551_PSEAE","entryAudit":{"firstPublicDate":"1986-07-21","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"1990-04-01","entryVersion":175,"sequenceVersion":2},"annotationScore":4.0,"organism":{"scientificName":"Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)","taxonId":208964,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Pseudomonadales","Pseudomonadaceae","Pseudomonas"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Cytochrome c-551"}},"alternativeNames":[{"fullName":{"value":"Cytochrome C8"}},{"fullName":{"value":"Cytochrome c551"}}],"flag":"Precursor"},"genes":[{"geneName":{"value":"nirM"},"orderedLocusNames":[{"value":"PA0518"}]}],"comments":[{"texts":[{"value":"Electron donor for cytochrome cd1 in nitrite and nitrate respiration"}],"commentType":"FUNCTION"},{"commentType":"BIOPHYSICOCHEMICAL PROPERTIES","redoxPotential":{"texts":[{"value":"E(0) is about +260 mV."}]}},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"value":"Periplasm","id":"SL-0200"}}]},{"texts":[{"value":"Binds 1 heme c group covalently per subunit"}],"commentType":"PTM"}],"features":[{"type":"Signal","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":22,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"14084622"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"4362497"}]},{"type":"Chain","location":{"start":{"value":23,"modifier":"EXACT"},"end":{"value":104,"modifier":"EXACT"}},"description":"Cytochrome c-551","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"2152881"}],"featureId":"PRO_0000006527"},{"type":"Binding site","location":{"start":{"value":34,"modifier":"EXACT"},"end":{"value":34,"modifier":"EXACT"}},"description":"covalent","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:61717"}],"ligand":{"name":"heme c","id":"ChEBI:CHEBI:61717"}},{"type":"Binding site","location":{"start":{"value":37,"modifier":"EXACT"},"end":{"value":37,"modifier":"EXACT"}},"description":"covalent","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:61717"}],"ligand":{"name":"heme c","id":"ChEBI:CHEBI:61717"}},{"type":"Binding site","location":{"start":{"value":38,"modifier":"EXACT"},"end":{"value":38,"modifier":"EXACT"}},"description":"axial binding residue","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:61717"},{"database":"ChEBI","id":"CHEBI:18248"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00433"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"6283101"}],"ligand":{"name":"heme c","id":"ChEBI:CHEBI:61717"},"ligandPart":{"name":"Fe","id":"ChEBI:CHEBI:18248"}},{"type":"Binding site","location":{"start":{"value":83,"modifier":"EXACT"},"end":{"value":83,"modifier":"EXACT"}},"description":"axial binding residue","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:61717"},{"database":"ChEBI","id":"CHEBI:18248"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00433"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"6283101"}],"ligand":{"name":"heme c","id":"ChEBI:CHEBI:61717"},"ligandPart":{"name":"Fe","id":"ChEBI:CHEBI:18248"}},{"type":"Natural variant","location":{"start":{"value":24,"modifier":"EXACT"},"end":{"value":24,"modifier":"EXACT"}},"description":"in strain: 129/1224","featureId":"","alternativeSequence":{"originalSequence":"D","alternativeSequences":["A"]}},{"type":"Helix","location":{"start":{"value":25,"modifier":"EXACT"},"end":{"value":31,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5XEC"}]},{"type":"Helix","location":{"start":{"value":34,"modifier":"EXACT"},"end":{"value":36,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5XEC"}]},{"type":"Turn","location":{"start":{"value":39,"modifier":"EXACT"},"end":{"value":41,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2PAC"}]},{"type":"Helix","location":{"start":{"value":42,"modifier":"EXACT"},"end":{"value":44,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5XED"}]},{"type":"Helix","location":{"start":{"value":49,"modifier":"EXACT"},"end":{"value":56,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5XEC"}]},{"type":"Helix","location":{"start":{"value":62,"modifier":"EXACT"},"end":{"value":72,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5XEC"}]},{"type":"Beta strand","location":{"start":{"value":76,"modifier":"EXACT"},"end":{"value":81,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5XEC"}]},{"type":"Helix","location":{"start":{"value":84,"modifier":"EXACT"},"end":{"value":86,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"351C"}]},{"type":"Helix","location":{"start":{"value":90,"modifier":"EXACT"},"end":{"value":101,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5XEC"}]}],"keywords":[{"id":"KW-0002","category":"Technical term","name":"3D-structure"},{"id":"KW-0903","category":"Technical term","name":"Direct protein sequencing"},{"id":"KW-0249","category":"Biological process","name":"Electron transport"},{"id":"KW-0349","category":"Ligand","name":"Heme"},{"id":"KW-0408","category":"Ligand","name":"Iron"},{"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"id":"KW-0574","category":"Cellular component","name":"Periplasm"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0732","category":"Domain","name":"Signal"},{"id":"KW-0813","category":"Biological process","name":"Transport"}],"references":[{"referenceNumber":1,"citation":{"id":"2152881","citationType":"journal article","authors":["Nordling M.","Young S.","Karlsson B.G.","Lundberg L.G."],"citationCrossReferences":[{"database":"PubMed","id":"2152881"},{"database":"DOI","id":"10.1016/0014-5793(90)80015-b"}],"title":"The structural gene for cytochrome c551 from Pseudomonas aeruginosa. The nucleotide sequence shows a location downstream of the nitrite reductase gene.","publicationDate":"1990","journal":"FEBS Lett.","firstPage":"230","lastPage":"232","volume":"259"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"],"referenceComments":[{"value":"ATCC 10145 / DSM 50071 / JCM 5962 / LMG 1242 / NBRC 12689 / NCIMB 8295 / NRRL B-771","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"2155133","citationType":"journal article","authors":["Arai H.","Sanbongi Y.","Igarashi Y.","Kodama T."],"citationCrossReferences":[{"database":"PubMed","id":"2155133"},{"database":"DOI","id":"10.1016/0014-5793(90)80669-a"}],"title":"Cloning and sequencing of the gene encoding cytochrome c-551 from Pseudomonas aeruginosa.","publicationDate":"1990","journal":"FEBS Lett.","firstPage":"196","lastPage":"198","volume":"261"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"]},{"referenceNumber":3,"citation":{"id":"10984043","citationType":"journal article","authors":["Stover C.K.","Pham X.-Q.T.","Erwin A.L.","Mizoguchi S.D.","Warrener P.","Hickey M.J.","Brinkman F.S.L.","Hufnagle W.O.","Kowalik D.J.","Lagrou M.","Garber R.L.","Goltry L.","Tolentino E.","Westbrock-Wadman S.","Yuan Y.","Brody L.L.","Coulter S.N.","Folger K.R.","Kas A.","Larbig K.","Lim R.M.","Smith K.A.","Spencer D.H.","Wong G.K.-S.","Wu Z.","Paulsen I.T.","Reizer J.","Saier M.H. Jr.","Hancock R.E.W.","Lory S.","Olson M.V."],"citationCrossReferences":[{"database":"PubMed","id":"10984043"},{"database":"DOI","id":"10.1038/35023079"}],"title":"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen.","publicationDate":"2000","journal":"Nature","firstPage":"959","lastPage":"964","volume":"406"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1","type":"STRAIN"}]},{"referenceNumber":4,"citation":{"id":"14084622","citationType":"journal article","authors":["Ambler R.P."],"citationCrossReferences":[{"database":"PubMed","id":"14084622"},{"database":"DOI","id":"10.1042/bj0890349"}],"title":"The amino acid sequence of Pseudomonas cytochrome c-551.","publicationDate":"1963","journal":"Biochem. J.","firstPage":"349","lastPage":"378","volume":"89"},"referencePositions":["PROTEIN SEQUENCE OF 23-104"],"referenceComments":[{"value":"P6009","type":"STRAIN"}]},{"referenceNumber":5,"citation":{"id":"4362497","citationType":"journal article","authors":["Ambler R.P."],"citationCrossReferences":[{"database":"PubMed","id":"4362497"},{"database":"DOI","id":"10.1042/bj1370003"}],"title":"The evolutionary stability of cytochrome c-551 in Pseudomonas aeruginosa and Pseudomonas fluorescens biotype C.","publicationDate":"1974","journal":"Biochem. J.","firstPage":"3","lastPage":"14","volume":"137"},"referencePositions":["PROTEIN SEQUENCE OF 23-104"],"referenceComments":[{"value":"Various strains","type":"STRAIN"}]},{"referenceNumber":6,"citation":{"id":"6283101","citationType":"journal article","authors":["Matsuura Y.","Takano T.","Dickerson R.E."],"citationCrossReferences":[{"database":"PubMed","id":"6283101"},{"database":"DOI","id":"10.1016/0022-2836(82)90335-7"}],"title":"Structure of cytochrome c551 from Pseudomonas aeruginosa refined at 1.6-A resolution and comparison of the two redox forms.","publicationDate":"1982","journal":"J. Mol. Biol.","firstPage":"389","lastPage":"409","volume":"156"},"referencePositions":["X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS)"]},{"referenceNumber":7,"citation":{"id":"96440","citationType":"journal article","authors":["Almassy R.J.","Dickerson R.E."],"citationCrossReferences":[{"database":"PubMed","id":"96440"},{"database":"DOI","id":"10.1073/pnas.75.6.2674"}],"title":"Pseudomonas cytochrome c551 at 2.0-A resolution: enlargement of the cytochrome c family.","publicationDate":"1978","journal":"Proc. Natl. Acad. Sci. U.S.A.","firstPage":"2674","lastPage":"2678","volume":"75"},"referencePositions":["X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS)"]},{"referenceNumber":8,"citation":{"id":"2174259","citationType":"journal article","authors":["Detlefsen D.J.","Thanabal V.","Pecoraro V.L.","Wagner G."],"citationCrossReferences":[{"database":"PubMed","id":"2174259"},{"database":"DOI","id":"10.1021/bi00492a011"}],"title":"Sequential 1H NMR assignments of iron(II) cytochrome c551 from Pseudomonas aeruginosa.","publicationDate":"1990","journal":"Biochemistry","firstPage":"9377","lastPage":"9386","volume":"29"},"referencePositions":["STRUCTURE BY NMR"]},{"referenceNumber":9,"citation":{"id":"1654086","citationType":"journal article","authors":["Detlefsen D.J.","Thanabal V.","Pecoraro V.L.","Wagner G."],"citationCrossReferences":[{"database":"PubMed","id":"1654086"},{"database":"DOI","id":"10.1021/bi00101a019"}],"title":"Solution structure of Fe(II) cytochrome c551 from Pseudomonas aeruginosa as determined by two-dimensional 1H NMR.","publicationDate":"1991","journal":"Biochemistry","firstPage":"9040","lastPage":"9046","volume":"30"},"referencePositions":["STRUCTURE BY NMR"]},{"referenceNumber":10,"citation":{"id":"8218218","citationType":"journal article","authors":["Timkovich R.","Cai M."],"citationCrossReferences":[{"database":"PubMed","id":"8218218"},{"database":"DOI","id":"10.1021/bi00094a007"}],"title":"Investigation of the structure of oxidized Pseudomonas aeruginosa cytochrome c-551 by NMR: comparison of observed paramagnetic shifts and calculated pseudocontact shifts.","publicationDate":"1993","journal":"Biochemistry","firstPage":"11516","lastPage":"11523","volume":"32"},"referencePositions":["STRUCTURE BY NMR"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"X51319","properties":[{"key":"ProteinId","value":"CAA35703.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"X51631","properties":[{"key":"ProteinId","value":"CAA35958.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AE004091","properties":[{"key":"ProteinId","value":"AAG03907.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"B34141","properties":[{"key":"EntryName","value":"CCPS5A"}]},{"database":"RefSeq","id":"NP_249209.1","properties":[{"key":"NucleotideSequenceId","value":"NC_002516.2"}]},{"database":"RefSeq","id":"WP_003084872.1","properties":[{"key":"NucleotideSequenceId","value":"NC_002516.2"}]},{"database":"PDB","id":"1DVV","properties":[{"key":"Method","value":"NMR"},{"key":"Resolution","value":"-"},{"key":"Chains","value":"A=23-104"}]},{"database":"PDB","id":"2EXV","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.86 A"},{"key":"Chains","value":"A/C=23-104"}]},{"database":"PDB","id":"2PAC","properties":[{"key":"Method","value":"NMR"},{"key":"Resolution","value":"-"},{"key":"Chains","value":"A=23-104"}]},{"database":"PDB","id":"351C","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.60 A"},{"key":"Chains","value":"A=23-104"}]},{"database":"PDB","id":"3X39","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.50 A"},{"key":"Chains","value":"A/B=23-104"}]},{"database":"PDB","id":"451C","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.60 A"},{"key":"Chains","value":"A=23-104"}]},{"database":"PDB","id":"5XEC","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.10 A"},{"key":"Chains","value":"A=23-42, C=43-104"}]},{"database":"PDB","id":"5XED","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.55 A"},{"key":"Chains","value":"A=23-42, C=43-104"}]},{"database":"PDBsum","id":"1DVV","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"2EXV","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"2PAC","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"351C","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"3X39","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"451C","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"5XEC","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"5XED","properties":[{"key":"Description","value":"-"}]},{"database":"AlphaFoldDB","id":"P00099","properties":[{"key":"Description","value":"-"}]},{"database":"BMRB","id":"P00099","properties":[{"key":"Description","value":"-"}]},{"database":"MDposit","id":"P00099","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"P00099","properties":[{"key":"Description","value":"-"}]},{"database":"STRING","id":"208964.PA0518","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"208964-PA0518","properties":[{"key":"Description","value":"-"}]},{"database":"DNASU","id":"882220","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"882220","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"pae:PA0518","properties":[{"key":"Description","value":"-"}]},{"database":"PATRIC","id":"fig|208964.12.peg.548","properties":[{"key":"GeneDesignation","value":"-"}]},{"database":"PseudoCAP","id":"PA0518","properties":[{"key":"Description","value":"-"}]},{"database":"HOGENOM","id":"CLU_133112_1_0_6","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"P00099","properties":[{"key":"Description","value":"-"}]},{"database":"OrthoDB","id":"9814063at2","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"PAER208964:G1FZ6-523-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"EvolutionaryTrace","id":"P00099","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000002438","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"AbasyAtlas","id":"P00099","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0042597","properties":[{"key":"GoTerm","value":"C:periplasmic space"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0009055","properties":[{"key":"GoTerm","value":"F:electron transfer activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0020037","properties":[{"key":"GoTerm","value":"F:heme binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0005506","properties":[{"key":"GoTerm","value":"F:iron ion binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"Gene3D","id":"1.10.760.10","properties":[{"key":"EntryName","value":"Cytochrome c-like domain"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR009056","properties":[{"key":"EntryName","value":"Cyt_c-like_dom"}]},{"database":"InterPro","id":"IPR036909","properties":[{"key":"EntryName","value":"Cyt_c-like_dom_sf"}]},{"database":"InterPro","id":"IPR002324","properties":[{"key":"EntryName","value":"Cyt_c_ID"}]},{"database":"Pfam","id":"PF00034","properties":[{"key":"EntryName","value":"Cytochrom_C"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR00606","properties":[{"key":"EntryName","value":"CYTCHROMECID"}]},{"database":"SUPFAM","id":"SSF46626","properties":[{"key":"EntryName","value":"Cytochrome c"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51007","properties":[{"key":"EntryName","value":"CYTC"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MKPYALLSLLATGTLLAQGAWAEDPEVLFKNKGCVACHAIDTKMVGPAYKDVAAKFAGQAGAEAELAQRIKNGSQGVWGPIPMPPNAVSDDEAQTLAKWVLSQK","length":104,"molWeight":10967,"crc64":"3AA94749BCEE4A94","md5":"0C22EB0A87EA5A6E9F8FC615A0473325"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"BIOPHYSICOCHEMICAL PROPERTIES":1,"SUBCELLULAR LOCATION":1,"PTM":1},"countByFeatureType":{"Signal":1,"Chain":1,"Binding site":4,"Natural variant":1,"Helix":7,"Turn":1,"Beta strand":1},"uniParcId":"UPI0000126C4A"}}