{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"O96935","secondaryAccessions":["Q8IEK1"],"uniProtkbId":"AMPN_PLAF7","entryAudit":{"firstPublicDate":"2002-02-21","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2003-05-30","entryVersion":138,"sequenceVersion":2},"annotationScore":5.0,"organism":{"scientificName":"Plasmodium falciparum (isolate 3D7)","taxonId":36329,"evidences":[{"evidenceCode":"ECO:0000312","source":"Proteomes","id":"UP000001450"}],"lineage":["Eukaryota","Sar","Alveolata","Apicomplexa","Aconoidasida","Haemosporida","Plasmodiidae","Plasmodium","Laverania"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"17895246"}],"value":"Aminopeptidase N"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12166515"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17895246"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20591164"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21659511"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22359643"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34133730"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9879894"}],"value":"3.4.11.-"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"19196988"}],"value":"M1 alanyl aminopeptidase"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"22359643"}],"value":"PfM1AAP"}]},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"9879894"}],"value":"M1 family aminopeptidase"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"12166515"}],"value":"PfA-M1"}}],"contains":[{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"12166515"}],"value":"p120 form"}}},{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"9879894"}],"value":"p96 form"}}},{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"9879894"}],"value":"p68 form"}}},{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"21659511"}],"value":"p35 form"}}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"22359643"}],"value":"M1AAP"},"orfNames":[{"value":"MAL13P1.56"},{"evidences":[{"evidenceCode":"ECO:0000312","source":"EMBL","id":"CAD52253.1"}],"value":"PF3D7_1311800"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12166515"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"19196988"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21659511"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21844374"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22359643"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23897806"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"33536500"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34133730"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9879894"}],"value":"Displays aminopeptidase activity with a broad substrate specificity (PubMed:12166515, PubMed:19196988, PubMed:21659511, PubMed:21844374, PubMed:22359643, PubMed:23897806, PubMed:33536500, PubMed:34133730, PubMed:9879894). Preferentially, cleaves after Leu and Met, but also cleaves after Ala and Arg (PubMed:12166515, PubMed:21844374, PubMed:22359643, PubMed:33536500). Low activity towards Lys, Phe, Tyr, Trp, Gln, Ser and Gly and negligible activity towards Glu, Asp, Pro, Ile, Thr, Val, His and Asn (PubMed:22359643). Has dipeptidase activity (PubMed:21659511, PubMed:23897806). Plays a role in the terminal stages of host hemoglobin digestion by cleaving the N-terminal residue of small hemoglobin-derived oligopeptides (PubMed:21659511, PubMed:21844374, PubMed:34133730)"}],"commentType":"FUNCTION"},{"commentType":"COFACTOR","cofactors":[{"name":"Zn(2+)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12166515"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"19196988"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21366301"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:29105"}}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12166515"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"19196988"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21366301"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21844374"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23713488"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23897806"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25299353"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25645579"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"26406322"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"26807544"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30537832"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32182520"},{"evidenceCode":"ECO:0000269","source":"Reference","id":"Ref.21"},{"evidenceCode":"ECO:0000269","source":"Reference","id":"Ref.24"}],"value":"Binds 1 zinc ion per subunit (PubMed:12166515, PubMed:19196988, PubMed:21366301, PubMed:21844374, PubMed:23713488, PubMed:23897806, PubMed:25299353, PubMed:25645579, PubMed:26406322, PubMed:26807544, PubMed:30537832, PubMed:32182520, Ref.21, Ref.24). Also, can use Mn(2+), Mg(2+) and Co(2+) with less efficiency (PubMed:12166515)."}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12166515"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"19196988"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21659511"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21844374"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34133730"}],"value":"Inhibited by 1,10-phenanthroline, EDTA and bestatin (PubMed:12166515, PubMed:19196988, PubMed:21659511, PubMed:34133730). Inhibited by (Benzyl)Tyr-Ala (BTA) (PubMed:21844374). Activity is not affected by phosphoramidin, PMSF, leupeptin, iodoacetamide or pepstatin (PubMed:12166515, PubMed:19196988)"}],"commentType":"ACTIVITY REGULATION"},{"commentType":"BIOPHYSICOCHEMICAL PROPERTIES","molecule":"p120 form","kineticParameters":{"michaelisConstants":[{"constant":110.0,"unit":"uM","substrate":"Arg-betaNA (at pH 7.5 and 27 degrees Celsius)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21659511"}]},{"constant":170.0,"unit":"uM","substrate":"Arg-betaNA (at pH 7.5 and 27 degrees Celsius, recombinant protein)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21659511"}]},{"constant":1100.0,"unit":"uM","substrate":"Arg-betaNA (at pH 5.5 and 27 degrees Celsius, recombinant protein)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21659511"}]},{"constant":210.0,"unit":"uM","substrate":"Ala-betaNA (at pH 7.5 and 27 degrees Celsius, recombinant protein)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21659511"}]},{"constant":4400.0,"unit":"uM","substrate":"Ala-betaNA (at pH 5.5 and 27 degrees Celsius, recombinant protein)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21659511"}]},{"constant":22.0,"unit":"uM","substrate":"Met-Phe (at pH 7.5 and 27 degrees Celsius, recombinant protein)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21659511"}]},{"constant":65.0,"unit":"uM","substrate":"Met-Phe (at pH 5.5 and 27 degrees Celsius, recombinant protein)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21659511"}]},{"constant":380.0,"unit":"uM","substrate":"Met-Phe (at pH 5 and 27 degrees Celsius, recombinant protein)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21659511"}]},{"constant":130.0,"unit":"uM","substrate":"Leu-Leu (at pH 7.5 and 27 degrees Celsius, recombinant protein)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21659511"}]},{"constant":410.0,"unit":"uM","substrate":"Leu-Leu (at pH 5.5 and 27 degrees Celsius, recombinant protein)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21659511"}]},{"constant":1200.0,"unit":"uM","substrate":"Leu-Leu (at pH 5 and 27 degrees Celsius, recombinant protein)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21659511"}]}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21659511"}],"value":"kcat is 9.3 sec(-1) with for Arg-betaNA as substrate (at pH 7.5 and 37 degrees Celsius) (PubMed:21659511). kcat is 21 sec(-1) with for Arg-betaNA as substrate (at pH 7.5 and 37 degrees Celsius, recombinant protein) (PubMed:21659511). kcat is 9.4 sec(-1) with for Arg-betaNA as substrate (at pH 5.5 and 37 degrees Celsius, recombinant protein) (PubMed:21659511). kcat is 36 sec(-1) with for Ala-betaNA as substrate (at pH 7.5 and 37 degrees Celsius, recombinant protein) (PubMed:21659511). kcat is 16 sec(-1) with for Ala-betaNA as substrate (at pH 5.5 and 37 degrees Celsius, recombinant protein) (PubMed:21659511). kcat is 5.9 sec(-1) with for Met-Phe as substrate (at pH 7.5 and 37 degrees Celsius, recombinant protein) (PubMed:21659511). kcat is 7.5 sec(-1) with for Met-Phe as substrate (at pH 5.5 and 37 degrees Celsius, recombinant protein) (PubMed:21659511). kcat is 7.4 sec(-1) with for Met-Phe as substrate (at pH 5 and 37 degrees Celsius, recombinant protein) (PubMed:21659511). kcat is 12 sec(-1) with for Leu-Leu as substrate (at pH 7.5 and 37 degrees Celsius, recombinant protein) (PubMed:21659511). kcat is 7.9 sec(-1) with for Leu-Leu as substrate (at pH 5.5 and 37 degrees Celsius, recombinant protein) (PubMed:21659511). kcat is 1.4 sec(-1) with for Leu-Leu as substrate (at pH 5 and 37 degrees Celsius, recombinant protein) (PubMed:21659511)."}]}},"phDependence":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21659511"}],"value":"Optimum pH is 7 (PubMed:21659511). Active between 5-8.5 (PubMed:21659511)."}]}},{"commentType":"BIOPHYSICOCHEMICAL PROPERTIES","molecule":"p68 form","kineticParameters":{"michaelisConstants":[{"constant":86.0,"unit":"uM","substrate":"Arg-betaNA (at pH 7.5 and 27 degrees Celsius)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21659511"}]}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21659511"}],"value":"kcat is 11 sec(-1) with for Arg-betaNA as substrate (at pH 7.5 and 37 degrees Celsius)."}]}}},{"commentType":"BIOPHYSICOCHEMICAL PROPERTIES","kineticParameters":{"michaelisConstants":[{"constant":197.0,"unit":"uM","substrate":"H-Ala-NHMec (at pH 7.5 and 37 degrees Celsius)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"33536500"}]},{"constant":1276.0,"unit":"uM","substrate":"H-Ala-NHMec (at pH 5.2 and 37 degrees Celsius)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"33536500"}]},{"constant":197.0,"unit":"uM","substrate":"H-Leu-NHMec (at pH 7.5 and 37 degrees Celsius)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"33536500"}]},{"constant":1714.0,"unit":"uM","substrate":"H-Leu-NHMec (at pH 5.2 and 37 degrees Celsius)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"33536500"}]},{"constant":308.0,"unit":"uM","substrate":"H-Arg-NHMec (at pH 7.5 and 37 degrees Celsius)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"33536500"}]},{"constant":980.0,"unit":"uM","substrate":"H-Arg-NHMec (at pH 5.2 and 37 degrees Celsius)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"33536500"}]},{"constant":240.6,"unit":"uM","substrate":"Ala-ACC (at pH 8, at 37 degrees Celsius and in the presence of Co(2+))","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22359643"}]},{"constant":214.3,"unit":"uM","substrate":"Arg-ACC (at pH 8, at 37 degrees Celsius and in the presence of Co(2+))","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22359643"}]},{"constant":140.9,"unit":"uM","substrate":"Leu-ACC (at pH 8, at 37 degrees Celsius and in the presence of Co(2+))","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22359643"}]},{"constant":218.0,"unit":"uM","substrate":"Phe-ACC (at pH 8, at 37 degrees Celsius and in the presence of Co(2+))","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22359643"}]},{"constant":138.2,"unit":"uM","substrate":"Met-ACC (at pH 8, at 37 degrees Celsius and in the presence of Co(2+))","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22359643"}]},{"constant":144.4,"unit":"uM","substrate":"Trp-ACC (at pH 8, at 37 degrees Celsius and in the presence of Co(2+))","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22359643"}]},{"constant":173.0,"unit":"uM","substrate":"Leu-Mec (at pH 8, at 37 degrees Celsius and in the presence of Co(2+))","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34133730"}]}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22359643"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"33536500"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34133730"}],"value":"kcat is 0.9 sec(-1) with for H-Ala-NHMec as substrate (at pH 7.5 and 37 degrees Celsius) (PubMed:33536500). kcat is 0.4 sec(-1) with for H-Ala-NHMec as substrate (at pH 5.2 and 37 degrees Celsius) (PubMed:33536500). kcat is 0.8 sec(-1) with for H-Leu-NHMec as substrate (at pH 7.5 and 37 degrees Celsius) (PubMed:33536500). kcat is 0.7 sec(-1) with for H-Leu-NHMec as substrate (at pH 5.2 and 37 degrees Celsius) (PubMed:33536500). kcat is 0.7 sec(-1) with for H-Arg-NHMec as substrate (at pH 7.5 and 37 degrees Celsius) (PubMed:33536500). kcat is 0.4 sec(-1) with for H-Arg-NHMec as substrate (at pH 5.2 and 37 degrees Celsius) (PubMed:33536500). kcat is 1.054 sec(-1) with for Ala-ACC as substrate (at pH 8, at 37 degrees Celsius and in the presence of Co(2+)) (PubMed:22359643). kcat is 0.876 sec(-1) with for Arg-ACC as substrate (at pH 8, at 37 degrees Celsius and in the presence of Co(2+)) (PubMed:22359643). kcat is 0.868 sec(-1) with for Leu-ACC as substrate (at pH 8, at 37 degrees Celsius and in the presence of Co(2+)) (PubMed:22359643). kcat is 0.422 sec(-1) with for Phe-ACC as substrate (at pH 8, at 37 degrees Celsius and in the presence of Co(2+)) (PubMed:22359643). kcat is 0.887 sec(-1) with for Met-ACC as substrate (at pH 8, at 37 degrees Celsius and in the presence of Co(2+)) (PubMed:22359643). kcat is 0.444 sec(-1) with for Trp-ACC as substrate (at pH 8, at 37 degrees Celsius and in the presence of Co(2+)) (PubMed:22359643). kcat is 0.12 sec(-1) with for Leu-Mec as substrate (at pH 8, at 37 degrees Celsius and in the presence of Co(2+)) (PubMed:34133730)."}]}},"phDependence":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"19196988"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20591164"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"33536500"}],"value":"Optimum pH is 7-7.5 (PubMed:19196988, PubMed:20591164, PubMed:33536500). Active from pH 5 to 9 (PubMed:19196988, PubMed:33536500). Has less than 20% of normal activity at pH 6.0 (PubMed:19196988)."}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21659511"}],"value":"Heterodimer of the p68 form and the p35 form which are derived from the p120 precursor"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","molecule":"Aminopeptidase N","note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20591164"}],"value":"In trophozoites, partially localizes to the parasitophorous vacuole membrane"}]},"subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20591164"}],"value":"Parasitophorous vacuole membrane","id":"SL-0193"},"topology":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Peripheral membrane protein","id":"SL-9903"}}]},{"commentType":"SUBCELLULAR LOCATION","molecule":"p120 form","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21659511"}],"value":"Nucleus","id":"SL-0191"}}]},{"commentType":"SUBCELLULAR LOCATION","molecule":"p96 form","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"33536500"}],"value":"Cytoplasm","id":"SL-0086"}}]},{"commentType":"SUBCELLULAR LOCATION","molecule":"p68 form","note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20591164"}],"value":"In trophozoites, partially localizes to the digestive (or food) vacuole, an acidic vacuole where host hemoglobin is digested"}]},"subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"33536500"}],"value":"Cytoplasm","id":"SL-0086"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17895246"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20591164"}],"value":"Vacuole lumen","id":"SL-0270"}}]},{"commentType":"SUBCELLULAR LOCATION","note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12166515"}],"value":"In trophozoite and schizonts, localizes to the cytoplasm (PubMed:12166515). In trophozoites, also localizes around the digestive vacuole (PubMed:12166515). In schizonts, also localizes to discrete vesicle-like structures and in released merozoites localizes to a single discrete structure (PubMed:12166515). In ruptured schizonts, localizes around the digestive vacuole (PubMed:12166515)"}]},"subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12166515"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9879894"}],"value":"Cytoplasm","id":"SL-0086"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12166515"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17895246"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"19196988"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20591164"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"33536500"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9879894"}],"value":"Expressed during the asexual blood stage in late rings, trophozoites and schizonts (at protein level) (PubMed:12166515, PubMed:17895246, PubMed:19196988, PubMed:20591164, PubMed:33536500, PubMed:9879894). No expression at the early ring stage (at protein level) (PubMed:12166515, PubMed:20591164)"}],"commentType":"DEVELOPMENTAL STAGE","molecule":"Aminopeptidase N"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20591164"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9879894"}],"value":"Expressed at low levels during the asexual blood stage in trophozoites and early schizonts (at protein level) (PubMed:20591164, PubMed:9879894). No expression at the early ring stage and in mature segmented schizonts (at protein level) (PubMed:20591164)"}],"commentType":"DEVELOPMENTAL STAGE","molecule":"p96 form"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12166515"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20591164"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21659511"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9879894"}],"value":"Expressed during the asexual blood stage in mature rings, trophozoites and schizonts (at protein level) (PubMed:12166515, PubMed:20591164, PubMed:21659511, PubMed:9879894). No expression at the early ring stage (at protein level) (PubMed:12166515, PubMed:20591164)"}],"commentType":"DEVELOPMENTAL STAGE","molecule":"p68 form"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12166515"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20591164"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21659511"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"33536500"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9879894"}],"value":"The full length protein appears to be cleaved into a 120 kDa precursor. This precursor is then proteolytically cleaved at the N-terminus generating a 96 kDa form which is further processed at the C-terminus into 68 kDa and 35 kDa forms that remain associated"}],"commentType":"PTM"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21659511"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"21659511"}],"value":"May have an additional translation initiation (PubMed:21659511). Translation initiation at the first Met codon would generate a form that contains the signal peptide, enters the endomembrane system, and ultimately reaches the food vacuole (PubMed:21659511). Initiation of translation at an internal Met codon would result in a form that lacks the signal peptide, thereby re-directing the protein to the cytosol (Probable). A nuclear localization signal would then lead to nuclear import (Probable)"}],"commentType":"MISCELLANEOUS"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the peptidase M1 family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12166515"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21659511"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"33536500"}],"value":"The cytoplasm localization is controversial. One study showed that the protein localizes predominantly to the nucleus and the food vacuole (PubMed:21659511). Two studies showed that it localizes to the cytoplasm and the food vacuole (PubMed:12166515, PubMed:33536500). The discrepancy between studies is due to the staining procedure (PubMed:21659511)"}],"commentType":"CAUTION"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1085,"modifier":"EXACT"}},"description":"Aminopeptidase N","featureId":"PRO_0000095100"},{"type":"Signal","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":30,"modifier":"EXACT"}},"description":"Required for ER targeting and membrane association; not cleaved","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20591164"}]},{"type":"Chain","location":{"start":{"value":null,"modifier":"UNKNOWN"},"end":{"value":795,"modifier":"EXACT"}},"description":"p68 form","evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"21659511"}],"featureId":"PRO_0000457921"},{"type":"Chain","location":{"start":{"value":796,"modifier":"EXACT"},"end":{"value":1085,"modifier":"EXACT"}},"description":"p35 form","evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"21659511"}],"featureId":"PRO_0000457922"},{"type":"Chain","location":{"start":{"value":null,"modifier":"UNKNOWN"},"end":{"value":1085,"modifier":"EXACT"}},"description":"p120 form","evidences":[{"evidenceCode":"ECO:0000305"}],"featureId":"PRO_0000457923"},{"type":"Chain","location":{"start":{"value":null,"modifier":"UNKNOWN"},"end":{"value":1085,"modifier":"EXACT"}},"description":"p96 form","evidences":[{"evidenceCode":"ECO:0000305"}],"featureId":"PRO_0000457924"},{"type":"Region","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":200,"modifier":"EXACT"}},"description":"Sufficient for targeting to the food 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