{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"O94874","secondaryAccessions":["A0PJ53","B4DJ57","C0H5X5","Q8N765","Q9NTQ0"],"uniProtkbId":"UFL1_HUMAN","entryAudit":{"firstPublicDate":"2005-06-21","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2005-06-21","entryVersion":181,"sequenceVersion":2},"annotationScore":5.0,"organism":{"scientificName":"Homo sapiens","commonName":"Human","taxonId":9606,"lineage":["Eukaryota","Metazoa","Chordata","Craniata","Vertebrata","Euteleostomi","Mammalia","Eutheria","Euarchontoglires","Primates","Haplorrhini","Catarrhini","Hominidae","Homo"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"E3 UFM1-protein ligase 1"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20018847"}],"value":"2.3.2.-"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"E3 UFM1-protein transferase 1"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"20531390"}],"value":"Multiple alpha-helix protein located at ER"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"20164180"}],"value":"Novel LZAP-binding protein"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"20228063"}],"value":"Regulator of C53/LZAP and DDRGK1"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"30354401"},{"evidenceCode":"ECO:0000312","source":"HGNC","id":"HGNC:23039"}],"value":"UFL1"},"synonyms":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"9872452"}],"value":"KIAA0776"},{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"20531390"}],"value":"MAXER"},{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"20164180"}],"value":"NLBP"},{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"20228063"}],"value":"RCAD"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8CCJ3"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20018847"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20164180"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20228063"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20531390"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23152784"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25219498"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27351204"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30626644"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30783677"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30886146"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32050156"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32160526"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32807901"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"35394863"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"35753586"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"36121123"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"36543799"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"36893266"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37036982"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37311461"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37595036"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37795761"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"38377992"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"38383785"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"38383789"}],"value":"E3 protein ligase that mediates ufmylation, the covalent attachment of the ubiquitin-like modifier UFM1 to lysine residues on target proteins, and which plays a key role in various processes, such as ribosome recycling, response to DNA damage, interferon response or reticulophagy (also called ER-phagy) (PubMed:20018847, PubMed:20164180, PubMed:20228063, PubMed:25219498, PubMed:27351204, PubMed:30626644, PubMed:30783677, PubMed:32160526, PubMed:32807901, PubMed:35394863, PubMed:36121123, PubMed:36543799, PubMed:36893266, PubMed:37036982, PubMed:37311461, PubMed:37595036, PubMed:37795761, PubMed:38377992, PubMed:38383785, PubMed:38383789). Catalyzes ufmylation of many protein, such as CD274/PD-L1, CDK5RAP3, CYB5R3, DDRGK1, EIF6, histone H4, MRE11, P4HB, PDCD1/PD-1, TRIP4, RPN1, RPS20/uS10, RPL10/uL16, RPL26/uL24, SYVN1/HRD1 and TP53/p53 (PubMed:20018847, PubMed:20531390, PubMed:25219498, PubMed:30783677, PubMed:30886146, PubMed:32160526, PubMed:35753586, PubMed:36543799, PubMed:36893266, PubMed:37036982, PubMed:37595036, PubMed:37795761, PubMed:38383785, PubMed:38383789). As part of the UREL complex, plays a key role in ribosome recycling by catalyzing mono-ufmylation of RPL26/uL24 subunit of the 60S ribosome (PubMed:38383785, PubMed:38383789). Ufmylation of RPL26/uL24 occurs on free 60S ribosomes following ribosome dissociation: it weakens the junction between post-termination 60S subunits and SEC61 translocons, promoting release and recycling of the large ribosomal subunit from the endoplasmic reticulum membrane (PubMed:38383785, PubMed:38383789). Ufmylation of RPL26/uL24 and subsequent 60S ribosome recycling either take place after normal termination of translation or after ribosome stalling during cotranslational translocation at the endoplasmic reticulum (PubMed:37036982, PubMed:37595036, PubMed:38383785, PubMed:38383789). Involved in reticulophagy in response to endoplasmic reticulum stress by mediating ufmylation of proteins such as CYB5R3 and RPN1, thereby promoting lysosomal degradation of ufmylated proteins (PubMed:23152784, PubMed:32160526, PubMed:36543799). Ufmylation in response to endoplasmic reticulum stress is essential for processes such as hematopoiesis, blood vessel morphogenesis or inflammatory response (PubMed:32050156). Mediates ufmylation of DDRGK1 and CDK5RAP3; the role of these modifications is however unclear: as both DDRGK1 and CDK5RAP3 act as substrate adapters for ufmylation, it is uncertain whether ufmylation of these proteins is, a collateral effect or is required for ufmylation (PubMed:20018847, PubMed:20531390). Acts as a negative regulator of T-cell activation by mediating ufmylation and stabilization of PDCD1/PD-1 (PubMed:38377992). Also involved in the response to DNA damage: recruited to double-strand break sites following DNA damage and mediates monoufmylation of histone H4 and ufmylation of MRE11 (PubMed:30783677, PubMed:30886146). Mediates ufmylation of TP53/p53, promoting its stability (PubMed:32807901). Catalyzes ufmylation of TRIP4, thereby playing a role in nuclear receptor-mediated transcription (PubMed:25219498). Required for hematopoietic stem cell function and hematopoiesis (By similarity)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20018847"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20164180"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20228063"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25219498"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30886146"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32160526"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"36121123"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"36543799"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37595036"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37988244"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"38377992"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"38383785"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"38383789"}],"value":"Catalytic component of the UFM1 ribosome E3 ligase (UREL) complex, composed of UFL1, DDRGK1 and CDK5RAP3 (PubMed:20018847, PubMed:20164180, PubMed:20228063, PubMed:25219498, PubMed:32160526, PubMed:36121123, PubMed:36543799, PubMed:37595036, PubMed:38383785, PubMed:38383789). Interacts with E2-like enzyme UFC1 (PubMed:20018847, PubMed:30886146, PubMed:37988244, PubMed:38383789). Interacts with RELA (PubMed:20164180). Interacts with NBN; promoting recruitment to double-strand breaks following DNA damage (PubMed:30886146). Interacts (when phosphorylated) with YWHAG/14-3-3-gamma; sequestering UFL1 and preventing its association with PDCD1/PD-1 substrate (PubMed:38377992)"}],"commentType":"SUBUNIT"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37311461"}],"value":"(Microbial infection) Interacts with Epstein-Barr virus protein BILF1; this interaction mediates MAVS UFMylation and subsequent routing from mitochondria to lysosomes"}],"commentType":"SUBUNIT"},{"commentType":"INTERACTION","interactions":[{"interactantOne":{"uniProtKBAccession":"O94874","intActId":"EBI-1048088"},"interactantTwo":{"uniProtKBAccession":"Q96JB5","geneName":"CDK5RAP3","intActId":"EBI-718818"},"numberOfExperiments":12,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"O94874","intActId":"EBI-1048088"},"interactantTwo":{"uniProtKBAccession":"Q96HY6","geneName":"DDRGK1","intActId":"EBI-1054024"},"numberOfExperiments":10,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"O94874","intActId":"EBI-1048088"},"interactantTwo":{"uniProtKBAccession":"Q9Y3C8","geneName":"UFC1","intActId":"EBI-1045733"},"numberOfExperiments":9,"organismDiffer":false}]},{"commentType":"SUBCELLULAR LOCATION","note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30886146"}],"value":"Recruited to double-strand breaks by the MRE11-RAD50-NBN (MRN) complex following DNA damage"}]},"subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20018847"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20164180"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20228063"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32160526"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"36543799"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"38383785"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"38383789"}],"value":"Endoplasmic reticulum membrane","id":"SL-0097"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20228063"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30886146"}],"value":"Cytoplasm, cytosol","id":"SL-0091"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30886146"}],"value":"Nucleus","id":"SL-0191"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30886146"}],"value":"Chromosome","id":"SL-0468"}}]},{"commentType":"ALTERNATIVE PRODUCTS","events":["Alternative splicing"],"isoforms":[{"name":{"value":"1"},"isoformIds":["O94874-1"],"isoformSequenceStatus":"Displayed"},{"name":{"value":"2"},"isoformIds":["O94874-2"],"sequenceIds":["VSP_038759"],"isoformSequenceStatus":"Described"},{"name":{"value":"3"},"isoformIds":["O94874-3"],"sequenceIds":["VSP_038760","VSP_038761"],"isoformSequenceStatus":"Described"}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20018847"}],"value":"Ubiquitously expressed, with a high expression in liver (at protein level) (PubMed:20018847). Low expression in several invasive hepatocellular carcinomas, such Hep-G2, Hep 3B2.1-7, HLE and PLC (PubMed:20018847)"}],"commentType":"TISSUE SPECIFICITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23152784"}],"value":"Up-regulated by thapsigargin (PubMed:23152784). Down-regulated in the failing hearts of patients with dilated cardiomyopathy (PubMed:23152784)"}],"commentType":"INDUCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20164180"}],"value":"Ubiquitinated, leading to its degradation by the proteasome (PubMed:20164180). Interaction with CDK5RAP3 protects both proteins against ubiquitination and degradation via the proteasome (PubMed:20164180)"}],"commentType":"PTM"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30886146"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"38377992"}],"value":"Phosphorylated at Ser-462 by ATM, enhancing protein ligase activity and promoting ATM activation in a positive feedback loop (PubMed:30886146). Phosphorylation at Thr-536 by AMPK promotes its interaction with YWHAG/14-3-3-gamma, thereby preventing UFL1 association with PDCD1/PD-1 substrate (PubMed:38377992)"}],"commentType":"PTM"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the UFL1 family"}],"commentType":"SIMILARITY"},{"commentType":"SEQUENCE CAUTION","sequenceCautionType":"Miscellaneous discrepancy","sequence":"AAH15377.1","note":"Contaminating sequence. Potential poly-A sequence.","evidences":[{"evidenceCode":"ECO:0000305"}]},{"commentType":"SEQUENCE CAUTION","sequenceCautionType":"Erroneous initiation","sequence":"BAA34496.1","note":"Extended N-terminus.","evidences":[{"evidenceCode":"ECO:0000305"}]}],"features":[{"type":"Initiator methionine","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"Removed","evidences":[{"evidenceCode":"ECO:0007744","source":"PubMed","id":"22223895"}]},{"type":"Chain","location":{"start":{"value":2,"modifier":"EXACT"},"end":{"value":794,"modifier":"EXACT"}},"description":"E3 UFM1-protein ligase 1","featureId":"PRO_0000050771"},{"type":"Region","location":{"start":{"value":2,"modifier":"EXACT"},"end":{"value":212,"modifier":"EXACT"}},"description":"Required for E3 UFM1-protein ligase activity","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20018847"}]},{"type":"Region","location":{"start":{"value":2,"modifier":"EXACT"},"end":{"value":200,"modifier":"EXACT"}},"description":"Mediates interaction with DDRGK1","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25219498"}]},{"type":"Region","location":{"start":{"value":121,"modifier":"EXACT"},"end":{"value":250,"modifier":"EXACT"}},"description":"Involved in CDK5RAP3-binding","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20164180"}]},{"type":"Region","location":{"start":{"value":200,"modifier":"EXACT"},"end":{"value":400,"modifier":"EXACT"}},"description":"Mediates interaction with TRIP4","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25219498"}]},{"type":"Region","location":{"start":{"value":407,"modifier":"EXACT"},"end":{"value":473,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":490,"modifier":"EXACT"},"end":{"value":684,"modifier":"EXACT"}},"description":"Mediates interaction with CDK5RAP3","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"B2GV24"}]},{"type":"Region","location":{"start":{"value":745,"modifier":"EXACT"},"end":{"value":770,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Modified residue","location":{"start":{"value":2,"modifier":"EXACT"},"end":{"value":2,"modifier":"EXACT"}},"description":"N-acetylalanine","evidences":[{"evidenceCode":"ECO:0007744","source":"PubMed","id":"22223895"}]},{"type":"Modified residue","location":{"start":{"value":433,"modifier":"EXACT"},"end":{"value":433,"modifier":"EXACT"}},"description":"Omega-N-methylarginine","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8CCJ3"}]},{"type":"Modified residue","location":{"start":{"value":458,"modifier":"EXACT"},"end":{"value":458,"modifier":"EXACT"}},"description":"Phosphoserine","evidences":[{"evidenceCode":"ECO:0007744","source":"PubMed","id":"17081983"},{"evidenceCode":"ECO:0007744","source":"PubMed","id":"18318008"},{"evidenceCode":"ECO:0007744","source":"PubMed","id":"20068231"},{"evidenceCode":"ECO:0007744","source":"PubMed","id":"21406692"},{"evidenceCode":"ECO:0007744","source":"PubMed","id":"24275569"}]},{"type":"Modified residue","location":{"start":{"value":462,"modifier":"EXACT"},"end":{"value":462,"modifier":"EXACT"}},"description":"Phosphoserine; by ATM","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30886146"}]},{"type":"Modified residue","location":{"start":{"value":536,"modifier":"EXACT"},"end":{"value":536,"modifier":"EXACT"}},"description":"Phosphothreonine; by AMPK","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"38377992"}]},{"type":"Alternative sequence","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":65,"modifier":"EXACT"}},"description":"in isoform 2","evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"14702039"}],"featureId":"VSP_038759","alternativeSequence":{}},{"type":"Alternative sequence","location":{"start":{"value":508,"modifier":"EXACT"},"end":{"value":509,"modifier":"EXACT"}},"description":"in isoform 3","evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"15489334"}],"featureId":"VSP_038760","alternativeSequence":{"originalSequence":"PL","alternativeSequences":["QV"]}},{"type":"Alternative sequence","location":{"start":{"value":510,"modifier":"EXACT"},"end":{"value":794,"modifier":"EXACT"}},"description":"in isoform 3","evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"15489334"}],"featureId":"VSP_038761","alternativeSequence":{}},{"type":"Natural variant","location":{"start":{"value":137,"modifier":"EXACT"},"end":{"value":137,"modifier":"EXACT"}},"description":"in dbSNP:rs28372909","featureCrossReferences":[{"database":"dbSNP","id":"rs28372909"}],"featureId":"VAR_034037","alternativeSequence":{"originalSequence":"V","alternativeSequences":["F"]}},{"type":"Mutagenesis","location":{"start":{"value":8,"modifier":"EXACT"},"end":{"value":8,"modifier":"EXACT"}},"description":"Abolished interaction with E2-like enzyme UFC1.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"38383789"}],"alternativeSequence":{"originalSequence":"I","alternativeSequences":["R"]}},{"type":"Mutagenesis","location":{"start":{"value":11,"modifier":"EXACT"},"end":{"value":15,"modifier":"EXACT"}},"description":"Abolished interaction with E2-like enzyme UFC1.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37988244"}],"alternativeSequence":{"originalSequence":"LAADF","alternativeSequences":["RAADR"]}},{"type":"Mutagenesis","location":{"start":{"value":15,"modifier":"EXACT"},"end":{"value":15,"modifier":"EXACT"}},"description":"Abolished interaction with E2-like enzyme UFC1.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"38383789"}],"alternativeSequence":{"originalSequence":"F","alternativeSequences":["R"]}},{"type":"Mutagenesis","location":{"start":{"value":19,"modifier":"EXACT"},"end":{"value":19,"modifier":"EXACT"}},"description":"Abolished interaction with E2-like enzyme UFC1.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"38383789"}],"alternativeSequence":{"originalSequence":"Q","alternativeSequences":["R"]}},{"type":"Mutagenesis","location":{"start":{"value":27,"modifier":"EXACT"},"end":{"value":27,"modifier":"EXACT"}},"description":"Abolished interaction with DDRGK1; when associated with A-32--A-39.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37595036"}],"alternativeSequence":{"originalSequence":"L","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":32,"modifier":"EXACT"},"end":{"value":39,"modifier":"EXACT"}},"description":"Abolished interaction with DDRGK1; when associated with A-27.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37595036"}],"alternativeSequence":{"originalSequence":"CIEIVNKL","alternativeSequences":["AIEAVNKA"]}},{"type":"Mutagenesis","location":{"start":{"value":32,"modifier":"EXACT"},"end":{"value":32,"modifier":"EXACT"}},"description":"Does not affect UFM1 ligase activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"36121123"}],"alternativeSequence":{"originalSequence":"C","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":125,"modifier":"EXACT"},"end":{"value":127,"modifier":"EXACT"}},"description":"Abolished interaction with UFC1, impairing E3 protein ligase activity and ability to regulate ATM activation.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30886146"}],"alternativeSequence":{}},{"type":"Mutagenesis","location":{"start":{"value":129,"modifier":"EXACT"},"end":{"value":129,"modifier":"EXACT"}},"description":"Abolished interaction with CDK5RAP3; when associated with A-185, A-191, A-199 and A-251.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37595036"}],"alternativeSequence":{"originalSequence":"D","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":143,"modifier":"EXACT"},"end":{"value":143,"modifier":"EXACT"}},"description":"Does not affect UFM1 ligase activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"36121123"}],"alternativeSequence":{"originalSequence":"C","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":185,"modifier":"EXACT"},"end":{"value":185,"modifier":"EXACT"}},"description":"Abolished interaction with CDK5RAP3; when associated with A-129, A-191, A-199 and A-251.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37595036"}],"alternativeSequence":{"originalSequence":"R","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":191,"modifier":"EXACT"},"end":{"value":191,"modifier":"EXACT"}},"description":"Abolished interaction with CDK5RAP3; when associated with A-129, A-185, A-199 and A-251.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37595036"}],"alternativeSequence":{"originalSequence":"R","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":199,"modifier":"EXACT"},"end":{"value":199,"modifier":"EXACT"}},"description":"Abolished interaction with CDK5RAP3; when associated with A-129, A-185, A-191 and A-251.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37595036"}],"alternativeSequence":{"originalSequence":"R","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":251,"modifier":"EXACT"},"end":{"value":251,"modifier":"EXACT"}},"description":"Abolished interaction with CDK5RAP3; when associated with A-129, A-185, A-191 and A-199.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37595036"}],"alternativeSequence":{"originalSequence":"R","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":300,"modifier":"EXACT"},"end":{"value":300,"modifier":"EXACT"}},"description":"Does not affect UFM1 ligase activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"36121123"}],"alternativeSequence":{"originalSequence":"C","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":372,"modifier":"EXACT"},"end":{"value":372,"modifier":"EXACT"}},"description":"Does not affect UFM1 ligase activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"36121123"}],"alternativeSequence":{"originalSequence":"C","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":462,"modifier":"EXACT"},"end":{"value":462,"modifier":"EXACT"}},"description":"Impaired recruitment to double-strand break sites.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30886146"}],"alternativeSequence":{"originalSequence":"S","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":536,"modifier":"EXACT"},"end":{"value":536,"modifier":"EXACT"}},"description":"Abolished phosphorylation by AMPK, preventing interaction with YWHAG/14-3-3-gamma and promoting PDCD1/PD-1 ufmylation.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"38377992"}],"alternativeSequence":{"originalSequence":"T","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":536,"modifier":"EXACT"},"end":{"value":536,"modifier":"EXACT"}},"description":"Mimics phosphorylation; leading to decreased PDCD1/PD-1 ufmylation.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"38377992"}],"alternativeSequence":{"originalSequence":"T","alternativeSequences":["E"]}},{"type":"Helix","location":{"start":{"value":2,"modifier":"EXACT"},"end":{"value":20,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8C0D"}]},{"type":"Helix","location":{"start":{"value":22,"modifier":"EXACT"},"end":{"value":25,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8C0D"}]},{"type":"Helix","location":{"start":{"value":29,"modifier":"EXACT"},"end":{"value":41,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8C0D"}]},{"type":"Beta strand","location":{"start":{"value":53,"modifier":"EXACT"},"end":{"value":57,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8C0D"}]},{"type":"Helix","location":{"start":{"value":59,"modifier":"EXACT"},"end":{"value":73,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8C0D"}]},{"type":"Beta strand","location":{"start":{"value":75,"modifier":"EXACT"},"end":{"value":78,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8C0D"}]},{"type":"Helix","location":{"start":{"value":79,"modifier":"EXACT"},"end":{"value":81,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8C0D"}]},{"type":"Helix","location":{"start":{"value":82,"modifier":"EXACT"},"end":{"value":86,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8C0D"}]},{"type":"Helix","location":{"start":{"value":90,"modifier":"EXACT"},"end":{"value":94,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8C0D"}]},{"type":"Helix","location":{"start":{"value":97,"modifier":"EXACT"},"end":{"value":104,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8C0D"}]},{"type":"Beta strand","location":{"start":{"value":105,"modifier":"EXACT"},"end":{"value":110,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8C0D"}]},{"type":"Beta strand","location":{"start":{"value":113,"modifier":"EXACT"},"end":{"value":116,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8C0D"}]},{"type":"Helix","location":{"start":{"value":117,"modifier":"EXACT"},"end":{"value":131,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8C0D"}]},{"type":"Beta strand","location":{"start":{"value":135,"modifier":"EXACT"},"end":{"value":138,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8OJ5"}]},{"type":"Helix","location":{"start":{"value":139,"modifier":"EXACT"},"end":{"value":145,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8C0D"}]},{"type":"Helix","location":{"start":{"value":150,"modifier":"EXACT"},"end":{"value":158,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8C0D"}]},{"type":"Turn","location":{"start":{"value":162,"modifier":"EXACT"},"end":{"value":164,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8OJ5"}]},{"type":"Beta strand","location":{"start":{"value":167,"modifier":"EXACT"},"end":{"value":169,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8OJ5"}]},{"type":"Beta strand","location":{"start":{"value":171,"modifier":"EXACT"},"end":{"value":174,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8OJ5"}]},{"type":"Beta strand","location":{"start":{"value":176,"modifier":"EXACT"},"end":{"value":178,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8OJ5"}]},{"type":"Helix","location":{"start":{"value":180,"modifier":"EXACT"},"end":{"value":196,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8OJ5"}]},{"type":"Helix","location":{"start":{"value":203,"modifier":"EXACT"},"end":{"value":210,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8OJ5"}]},{"type":"Helix","location":{"start":{"value":214,"modifier":"EXACT"},"end":{"value":227,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8OJ5"}]},{"type":"Beta strand","location":{"start":{"value":233,"modifier":"EXACT"},"end":{"value":235,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8OJ5"}]},{"type":"Beta strand","location":{"start":{"value":237,"modifier":"EXACT"},"end":{"value":241,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8OJ5"}]},{"type":"Beta strand","location":{"start":{"value":243,"modifier":"EXACT"},"end":{"value":245,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8OJ5"}]},{"type":"Helix","location":{"start":{"value":247,"modifier":"EXACT"},"end":{"value":264,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8OJ5"}]},{"type":"Beta strand","location":{"start":{"value":265,"modifier":"EXACT"},"end":{"value":268,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8OJ5"}]},{"type":"Helix","location":{"start":{"value":269,"modifier":"EXACT"},"end":{"value":275,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8OJ5"}]},{"type":"Helix","location":{"start":{"value":280,"modifier":"EXACT"},"end":{"value":287,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8OJ5"}]},{"type":"Turn","location":{"start":{"value":288,"modifier":"EXACT"},"end":{"value":290,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8OJ5"}]},{"type":"Beta strand","location":{"start":{"value":293,"modifier":"EXACT"},"end":{"value":295,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8OJ5"}]},{"type":"Beta strand","location":{"start":{"value":297,"modifier":"EXACT"},"end":{"value":302,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8OJ5"}]},{"type":"Helix","location":{"start":{"value":303,"modifier":"EXACT"},"end":{"value":319,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8OJ5"}]},{"type":"Beta strand","location":{"start":{"value":321,"modifier":"EXACT"},"end":{"value":324,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8OJ5"}]},{"type":"Helix","location":{"start":{"value":325,"modifier":"EXACT"},"end":{"value":328,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8OJ5"}]},{"type":"Helix","location":{"start":{"value":335,"modifier":"EXACT"},"end":{"value":346,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8OJ5"}]},{"type":"Helix","location":{"start":{"value":347,"modifier":"EXACT"},"end":{"value":349,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8OJ5"}]},{"type":"Turn","location":{"start":{"value":350,"modifier":"EXACT"},"end":{"value":353,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8OJ5"}]},{"type":"Beta strand","location":{"start":{"value":356,"modifier":"EXACT"},"end":{"value":358,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8QFC"}]},{"type":"Beta strand","location":{"start":{"value":359,"modifier":"EXACT"},"end":{"value":364,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8OJ5"}]},{"type":"Helix","location":{"start":{"value":366,"modifier":"EXACT"},"end":{"value":374,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8OJ5"}]},{"type":"Helix","location":{"start":{"value":377,"modifier":"EXACT"},"end":{"value":388,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8OJ5"}]},{"type":"Beta strand","location":{"start":{"value":394,"modifier":"EXACT"},"end":{"value":396,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"9GY4"}]},{"type":"Helix","location":{"start":{"value":417,"modifier":"EXACT"},"end":{"value":426,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8OJ5"}]},{"type":"Beta strand","location":{"start":{"value":431,"modifier":"EXACT"},"end":{"value":434,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8OJ5"}]},{"type":"Helix","location":{"start":{"value":440,"modifier":"EXACT"},"end":{"value":443,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8QFD"}]},{"type":"Helix","location":{"start":{"value":445,"modifier":"EXACT"},"end":{"value":447,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8OJ5"}]},{"type":"Helix","location":{"start":{"value":480,"modifier":"EXACT"},"end":{"value":486,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8OJ5"}]},{"type":"Helix","location":{"start":{"value":495,"modifier":"EXACT"},"end":{"value":520,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8OJ5"}]},{"type":"Helix","location":{"start":{"value":521,"modifier":"EXACT"},"end":{"value":525,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8OJ5"}]},{"type":"Turn","location":{"start":{"value":528,"modifier":"EXACT"},"end":{"value":530,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8OJ5"}]},{"type":"Helix","location":{"start":{"value":531,"modifier":"EXACT"},"end":{"value":559,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8OJ5"}]},{"type":"Turn","location":{"start":{"value":563,"modifier":"EXACT"},"end":{"value":565,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8OJ5"}]},{"type":"Helix","location":{"start":{"value":566,"modifier":"EXACT"},"end":{"value":573,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8OJ5"}]},{"type":"Helix","location":{"start":{"value":574,"modifier":"EXACT"},"end":{"value":578,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8OJ5"}]},{"type":"Helix","location":{"start":{"value":579,"modifier":"EXACT"},"end":{"value":592,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8OJ5"}]},{"type":"Helix","location":{"start":{"value":600,"modifier":"EXACT"},"end":{"value":602,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"9GY4"}]},{"type":"Helix","location":{"start":{"value":605,"modifier":"EXACT"},"end":{"value":614,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8OJ5"}]},{"type":"Helix","location":{"start":{"value":617,"modifier":"EXACT"},"end":{"value":631,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8OJ5"}]},{"type":"Helix","location":{"start":{"value":636,"modifier":"EXACT"},"end":{"value":648,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8OJ5"}]},{"type":"Helix","location":{"start":{"value":658,"modifier":"EXACT"},"end":{"value":678,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8OJ5"}]},{"type":"Helix","location":{"start":{"value":682,"modifier":"EXACT"},"end":{"value":698,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8OJ5"}]},{"type":"Helix","location":{"start":{"value":706,"modifier":"EXACT"},"end":{"value":708,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8OJ5"}]},{"type":"Helix","location":{"start":{"value":709,"modifier":"EXACT"},"end":{"value":716,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8OJ5"}]},{"type":"Helix","location":{"start":{"value":717,"modifier":"EXACT"},"end":{"value":719,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8OJ5"}]},{"type":"Helix","location":{"start":{"value":722,"modifier":"EXACT"},"end":{"value":746,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8OJ5"}]},{"type":"Helix","location":{"start":{"value":758,"modifier":"EXACT"},"end":{"value":763,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8OJ5"}]},{"type":"Helix","location":{"start":{"value":764,"modifier":"EXACT"},"end":{"value":768,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8OJ5"}]},{"type":"Helix","location":{"start":{"value":769,"modifier":"EXACT"},"end":{"value":782,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8OJ5"}]}],"keywords":[{"id":"KW-0002","category":"Technical term","name":"3D-structure"},{"id":"KW-0007","category":"PTM","name":"Acetylation"},{"id":"KW-0025","category":"Coding sequence diversity","name":"Alternative splicing"},{"id":"KW-0158","category":"Cellular component","name":"Chromosome"},{"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"id":"KW-0227","category":"Biological process","name":"DNA damage"},{"id":"KW-0234","category":"Biological process","name":"DNA repair"},{"id":"KW-0256","category":"Cellular component","name":"Endoplasmic reticulum"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-0488","category":"PTM","name":"Methylation"},{"id":"KW-0539","category":"Cellular component","name":"Nucleus"},{"id":"KW-0597","category":"PTM","name":"Phosphoprotein"},{"id":"KW-1267","category":"Technical term","name":"Proteomics identification"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0808","category":"Molecular function","name":"Transferase"},{"id":"KW-0832","category":"PTM","name":"Ubl conjugation"},{"id":"KW-0833","category":"Biological process","name":"Ubl conjugation pathway"}],"references":[{"referenceNumber":1,"citation":{"id":"9872452","citationType":"journal article","authors":["Nagase T.","Ishikawa K.","Suyama M.","Kikuno R.","Miyajima N.","Tanaka A.","Kotani H.","Nomura N.","Ohara O."],"citationCrossReferences":[{"database":"PubMed","id":"9872452"},{"database":"DOI","id":"10.1093/dnares/5.5.277"}],"title":"Prediction of the coding sequences of unidentified human genes. XI. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro.","publicationDate":"1998","journal":"DNA Res.","firstPage":"277","lastPage":"286","volume":"5"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)"],"referenceComments":[{"value":"Brain","type":"TISSUE"}]},{"referenceNumber":2,"citation":{"id":"14702039","citationType":"journal article","authors":["Ota T.","Suzuki Y.","Nishikawa T.","Otsuki T.","Sugiyama T.","Irie R.","Wakamatsu A.","Hayashi K.","Sato H.","Nagai K.","Kimura K.","Makita H.","Sekine M.","Obayashi M.","Nishi T.","Shibahara T.","Tanaka T.","Ishii S.","Yamamoto J.","Saito K.","Kawai Y.","Isono Y.","Nakamura Y.","Nagahari K.","Murakami K.","Yasuda T.","Iwayanagi T.","Wagatsuma M.","Shiratori A.","Sudo H.","Hosoiri T.","Kaku Y.","Kodaira H.","Kondo H.","Sugawara M.","Takahashi M.","Kanda K.","Yokoi T.","Furuya T.","Kikkawa E.","Omura Y.","Abe K.","Kamihara K.","Katsuta N.","Sato K.","Tanikawa M.","Yamazaki M.","Ninomiya K.","Ishibashi T.","Yamashita H.","Murakawa K.","Fujimori K.","Tanai H.","Kimata M.","Watanabe M.","Hiraoka S.","Chiba Y.","Ishida S.","Ono Y.","Takiguchi S.","Watanabe S.","Yosida M.","Hotuta T.","Kusano J.","Kanehori K.","Takahashi-Fujii A.","Hara H.","Tanase T.-O.","Nomura Y.","Togiya S.","Komai F.","Hara R.","Takeuchi K.","Arita M.","Imose N.","Musashino K.","Yuuki H.","Oshima A.","Sasaki N.","Aotsuka S.","Yoshikawa Y.","Matsunawa H.","Ichihara T.","Shiohata N.","Sano S.","Moriya S.","Momiyama H.","Satoh N.","Takami S.","Terashima Y.","Suzuki O.","Nakagawa S.","Senoh A.","Mizoguchi H.","Goto Y.","Shimizu F.","Wakebe H.","Hishigaki H.","Watanabe T.","Sugiyama A.","Takemoto M.","Kawakami B.","Yamazaki M.","Watanabe K.","Kumagai A.","Itakura S.","Fukuzumi Y.","Fujimori Y.","Komiyama M.","Tashiro H.","Tanigami A.","Fujiwara T.","Ono T.","Yamada K.","Fujii Y.","Ozaki K.","Hirao M.","Ohmori Y.","Kawabata A.","Hikiji T.","Kobatake N.","Inagaki H.","Ikema Y.","Okamoto S.","Okitani R.","Kawakami T.","Noguchi S.","Itoh T.","Shigeta K.","Senba T.","Matsumura K.","Nakajima Y.","Mizuno T.","Morinaga M.","Sasaki M.","Togashi T.","Oyama M.","Hata H.","Watanabe M.","Komatsu T.","Mizushima-Sugano J.","Satoh T.","Shirai Y.","Takahashi Y.","Nakagawa K.","Okumura K.","Nagase T.","Nomura N.","Kikuchi H.","Masuho Y.","Yamashita R.","Nakai K.","Yada T.","Nakamura Y.","Ohara O.","Isogai T.","Sugano S."],"citationCrossReferences":[{"database":"PubMed","id":"14702039"},{"database":"DOI","id":"10.1038/ng1285"}],"title":"Complete sequencing and characterization of 21,243 full-length human cDNAs.","publicationDate":"2004","journal":"Nat. Genet.","firstPage":"40","lastPage":"45","volume":"36"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2)"],"referenceComments":[{"value":"Substantia nigra","type":"TISSUE"}]},{"referenceNumber":3,"citation":{"id":"14574404","citationType":"journal article","authors":["Mungall A.J.","Palmer S.A.","Sims S.K.","Edwards C.A.","Ashurst J.L.","Wilming L.","Jones M.C.","Horton R.","Hunt S.E.","Scott C.E.","Gilbert J.G.R.","Clamp M.E.","Bethel G.","Milne S.","Ainscough R.","Almeida J.P.","Ambrose K.D.","Andrews T.D.","Ashwell R.I.S.","Babbage A.K.","Bagguley C.L.","Bailey J.","Banerjee R.","Barker D.J.","Barlow K.F.","Bates K.","Beare D.M.","Beasley H.","Beasley O.","Bird C.P.","Blakey S.E.","Bray-Allen S.","Brook J.","Brown A.J.","Brown J.Y.","Burford D.C.","Burrill W.","Burton J.","Carder C.","Carter N.P.","Chapman J.C.","Clark S.Y.","Clark G.","Clee C.M.","Clegg S.","Cobley V.","Collier R.E.","Collins J.E.","Colman L.K.","Corby N.R.","Coville G.J.","Culley K.M.","Dhami P.","Davies J.","Dunn M.","Earthrowl M.E.","Ellington A.E.","Evans K.A.","Faulkner L.","Francis M.D.","Frankish A.","Frankland J.","French L.","Garner P.","Garnett J.","Ghori M.J.","Gilby L.M.","Gillson C.J.","Glithero R.J.","Grafham D.V.","Grant M.","Gribble S.","Griffiths C.","Griffiths M.N.D.","Hall R.","Halls K.S.","Hammond S.","Harley J.L.","Hart E.A.","Heath P.D.","Heathcott R.","Holmes S.J.","Howden P.J.","Howe K.L.","Howell G.R.","Huckle E.","Humphray S.J.","Humphries M.D.","Hunt A.R.","Johnson C.M.","Joy A.A.","Kay M.","Keenan S.J.","Kimberley A.M.","King A.","Laird G.K.","Langford C.","Lawlor S.","Leongamornlert D.A.","Leversha M.","Lloyd C.R.","Lloyd D.M.","Loveland J.E.","Lovell J.","Martin S.","Mashreghi-Mohammadi M.","Maslen G.L.","Matthews L.","McCann O.T.","McLaren S.J.","McLay K.","McMurray A.","Moore M.J.F.","Mullikin J.C.","Niblett D.","Nickerson T.","Novik K.L.","Oliver K.","Overton-Larty E.K.","Parker A.","Patel R.","Pearce A.V.","Peck A.I.","Phillimore B.J.C.T.","Phillips S.","Plumb R.W.","Porter K.M.","Ramsey Y.","Ranby S.A.","Rice C.M.","Ross M.T.","Searle S.M.","Sehra H.K.","Sheridan E.","Skuce C.D.","Smith S.","Smith M.","Spraggon L.","Squares S.L.","Steward C.A.","Sycamore N.","Tamlyn-Hall G.","Tester J.","Theaker A.J.","Thomas D.W.","Thorpe A.","Tracey A.","Tromans A.","Tubby B.","Wall M.","Wallis J.M.","West A.P.","White S.S.","Whitehead S.L.","Whittaker H.","Wild A.","Willey D.J.","Wilmer T.E.","Wood J.M.","Wray P.W.","Wyatt J.C.","Young L.","Younger R.M.","Bentley D.R.","Coulson A.","Durbin R.M.","Hubbard T.","Sulston J.E.","Dunham I.","Rogers J.","Beck S."],"citationCrossReferences":[{"database":"PubMed","id":"14574404"},{"database":"DOI","id":"10.1038/nature02055"}],"title":"The DNA sequence and analysis of human chromosome 6.","publicationDate":"2003","journal":"Nature","firstPage":"805","lastPage":"811","volume":"425"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"]},{"referenceNumber":4,"citation":{"id":"CI-5GBD0VIIJ7C63","citationType":"submission","authors":["Mural R.J.","Istrail S.","Sutton G.G.","Florea L.","Halpern A.L.","Mobarry C.M.","Lippert R.","Walenz B.","Shatkay H.","Dew I.","Miller J.R.","Flanigan M.J.","Edwards N.J.","Bolanos R.","Fasulo D.","Halldorsson B.V.","Hannenhalli S.","Turner R.","Yooseph S.","Lu F.","Nusskern D.R.","Shue B.C.","Zheng X.H.","Zhong F.","Delcher A.L.","Huson D.H.","Kravitz S.A.","Mouchard L.","Reinert K.","Remington K.A.","Clark A.G.","Waterman M.S.","Eichler E.E.","Adams M.D.","Hunkapiller M.W.","Myers E.W.","Venter J.C."],"publicationDate":"SEP-2005","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"]},{"referenceNumber":5,"citation":{"id":"15489334","citationType":"journal article","authoringGroup":["The MGC Project Team"],"citationCrossReferences":[{"database":"PubMed","id":"15489334"},{"database":"DOI","id":"10.1101/gr.2596504"}],"title":"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).","publicationDate":"2004","journal":"Genome Res.","firstPage":"2121","lastPage":"2127","volume":"14"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3)"],"referenceComments":[{"value":"Kidney","type":"TISSUE"},{"value":"Testis","type":"TISSUE"}]},{"referenceNumber":6,"citation":{"id":"17081983","citationType":"journal article","authors":["Olsen J.V.","Blagoev B.","Gnad F.","Macek B.","Kumar C.","Mortensen P.","Mann M."],"citationCrossReferences":[{"database":"PubMed","id":"17081983"},{"database":"DOI","id":"10.1016/j.cell.2006.09.026"}],"title":"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks.","publicationDate":"2006","journal":"Cell","firstPage":"635","lastPage":"648","volume":"127"},"referencePositions":["PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-458","IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]"],"referenceComments":[{"value":"Cervix carcinoma","type":"TISSUE"}]},{"referenceNumber":7,"citation":{"id":"18318008","citationType":"journal article","authors":["Han G.","Ye M.","Zhou H.","Jiang X.","Feng S.","Jiang X.","Tian R.","Wan D.","Zou H.","Gu J."],"citationCrossReferences":[{"database":"PubMed","id":"18318008"},{"database":"DOI","id":"10.1002/pmic.200700884"}],"title":"Large-scale phosphoproteome analysis of human liver tissue by enrichment and fractionation of phosphopeptides with strong anion exchange chromatography.","publicationDate":"2008","journal":"Proteomics","firstPage":"1346","lastPage":"1361","volume":"8"},"referencePositions":["PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-458","IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]"],"referenceComments":[{"value":"Liver","type":"TISSUE"}]},{"referenceNumber":8,"citation":{"id":"20531390","citationType":"journal article","authors":["Shiwaku H.","Yoshimura N.","Tamura T.","Sone M.","Ogishima S.","Watase K.","Tagawa K.","Okazawa H."],"citationCrossReferences":[{"database":"PubMed","id":"20531390"},{"database":"DOI","id":"10.1038/emboj.2010.116"}],"title":"Suppression of the novel ER protein Maxer by mutant ataxin-1 in Bergman glia contributes to non-cell-autonomous toxicity.","publicationDate":"2010","journal":"EMBO J.","firstPage":"2446","lastPage":"2460","volume":"29"},"referencePositions":["FUNCTION"]},{"referenceNumber":9,"citation":{"id":"20018847","citationType":"journal article","authors":["Tatsumi K.","Sou Y.S.","Tada N.","Nakamura E.","Iemura S.","Natsume T.","Kang S.H.","Chung C.H.","Kasahara M.","Kominami E.","Yamamoto M.","Tanaka K.","Komatsu M."],"citationCrossReferences":[{"database":"PubMed","id":"20018847"},{"database":"DOI","id":"10.1074/jbc.m109.036814"}],"title":"A novel type of E3 ligase for the Ufm1 conjugation system.","publicationDate":"2010","journal":"J. Biol. Chem.","firstPage":"5417","lastPage":"5427","volume":"285"},"referencePositions":["IDENTIFICATION BY MASS SPECTROMETRY","FUNCTION","CATALYTIC ACTIVITY","SUBCELLULAR LOCATION","TISSUE SPECIFICITY","INTERACTION WITH DDRGK1 AND UFC1","REGION"]},{"referenceNumber":10,"citation":{"id":"20164180","citationType":"journal article","authors":["Kwon J.","Cho H.J.","Han S.H.","No J.G.","Kwon J.Y.","Kim H."],"citationCrossReferences":[{"database":"PubMed","id":"20164180"},{"database":"DOI","id":"10.1074/jbc.m109.065920"}],"title":"A novel LZAP-binding protein, NLBP, inhibits cell invasion.","publicationDate":"2010","journal":"J. Biol. Chem.","firstPage":"12232","lastPage":"12240","volume":"285"},"referencePositions":["IDENTIFICATION BY MASS SPECTROMETRY","UBIQUITINATION","FUNCTION","SUBCELLULAR LOCATION","INTERACTION WITH CDK5RAP3; DDRGK1 AND RELA","REGION"]},{"referenceNumber":11,"citation":{"id":"20228063","citationType":"journal article","authors":["Wu J.","Lei G.","Mei M.","Tang Y.","Li H."],"citationCrossReferences":[{"database":"PubMed","id":"20228063"},{"database":"DOI","id":"10.1074/jbc.m110.110619"}],"title":"A novel C53/LZAP-interacting protein regulates stability of C53/LZAP and DDRGK domain-containing Protein 1 (DDRGK1) and modulates NF-kappaB signaling.","publicationDate":"2010","journal":"J. Biol. Chem.","firstPage":"15126","lastPage":"15136","volume":"285"},"referencePositions":["FUNCTION","INTERACTION WITH CDK5RAP3","SUBCELLULAR LOCATION"]},{"referenceNumber":12,"citation":{"id":"20068231","citationType":"journal article","authors":["Olsen J.V.","Vermeulen M.","Santamaria A.","Kumar C.","Miller M.L.","Jensen L.J.","Gnad F.","Cox J.","Jensen T.S.","Nigg E.A.","Brunak S.","Mann M."],"citationCrossReferences":[{"database":"PubMed","id":"20068231"},{"database":"DOI","id":"10.1126/scisignal.2000475"}],"title":"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis.","publicationDate":"2010","journal":"Sci. Signal.","firstPage":"RA3","lastPage":"RA3","volume":"3"},"referencePositions":["PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-458","IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]"],"referenceComments":[{"value":"Cervix carcinoma","type":"TISSUE"}]},{"referenceNumber":13,"citation":{"id":"21269460","citationType":"journal article","authors":["Burkard T.R.","Planyavsky M.","Kaupe I.","Breitwieser F.P.","Buerckstuemmer T.","Bennett K.L.","Superti-Furga G.","Colinge J."],"citationCrossReferences":[{"database":"PubMed","id":"21269460"},{"database":"DOI","id":"10.1186/1752-0509-5-17"}],"title":"Initial characterization of the human central proteome.","publicationDate":"2011","journal":"BMC Syst. Biol.","firstPage":"17","lastPage":"17","volume":"5"},"referencePositions":["IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]"]},{"referenceNumber":14,"citation":{"id":"21406692","citationType":"journal article","authors":["Rigbolt K.T.","Prokhorova T.A.","Akimov V.","Henningsen J.","Johansen P.T.","Kratchmarova I.","Kassem M.","Mann M.","Olsen J.V.","Blagoev B."],"citationCrossReferences":[{"database":"PubMed","id":"21406692"},{"database":"DOI","id":"10.1126/scisignal.2001570"}],"title":"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.","publicationDate":"2011","journal":"Sci. Signal.","firstPage":"RS3","lastPage":"RS3","volume":"4"},"referencePositions":["PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-458","IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]"]},{"referenceNumber":15,"citation":{"id":"22223895","citationType":"journal article","authors":["Bienvenut W.V.","Sumpton D.","Martinez A.","Lilla S.","Espagne C.","Meinnel T.","Giglione C."],"citationCrossReferences":[{"database":"PubMed","id":"22223895"},{"database":"DOI","id":"10.1074/mcp.m111.015131"}],"title":"Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features.","publicationDate":"2012","journal":"Mol. Cell. Proteomics","firstPage":"M111.015131","lastPage":"M111.015131","volume":"11"},"referencePositions":["ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2","CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS]","IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]"]},{"referenceNumber":16,"citation":{"id":"23152784","citationType":"journal article","authors":["Zhang Y.","Zhang M.","Wu J.","Lei G.","Li H."],"citationCrossReferences":[{"database":"PubMed","id":"23152784"},{"database":"DOI","id":"10.1371/journal.pone.0048587"}],"title":"Transcriptional regulation of the Ufm1 conjugation system in response to disturbance of the endoplasmic reticulum homeostasis and inhibition of vesicle trafficking.","publicationDate":"2012","journal":"PLoS ONE","firstPage":"E48587","lastPage":"E48587","volume":"7"},"referencePositions":["FUNCTION","INDUCTION"]},{"referenceNumber":17,"citation":{"id":"23186163","citationType":"journal article","authors":["Zhou H.","Di Palma S.","Preisinger C.","Peng M.","Polat A.N.","Heck A.J.","Mohammed S."],"citationCrossReferences":[{"database":"PubMed","id":"23186163"},{"database":"DOI","id":"10.1021/pr300630k"}],"title":"Toward a comprehensive characterization of a human cancer cell phosphoproteome.","publicationDate":"2013","journal":"J. Proteome Res.","firstPage":"260","lastPage":"271","volume":"12"},"referencePositions":["IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]"],"referenceComments":[{"value":"Erythroleukemia","type":"TISSUE"}]},{"referenceNumber":18,"citation":{"id":"24275569","citationType":"journal article","authors":["Bian Y.","Song C.","Cheng K.","Dong M.","Wang F.","Huang J.","Sun D.","Wang L.","Ye M.","Zou H."],"citationCrossReferences":[{"database":"PubMed","id":"24275569"},{"database":"DOI","id":"10.1016/j.jprot.2013.11.014"}],"title":"An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.","publicationDate":"2014","journal":"J. Proteomics","firstPage":"253","lastPage":"262","volume":"96"},"referencePositions":["PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-458","IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]"],"referenceComments":[{"value":"Liver","type":"TISSUE"}]},{"referenceNumber":19,"citation":{"id":"25219498","citationType":"journal article","authors":["Yoo H.M.","Kang S.H.","Kim J.Y.","Lee J.E.","Seong M.W.","Lee S.W.","Ka S.H.","Sou Y.S.","Komatsu M.","Tanaka K.","Lee S.T.","Noh D.Y.","Baek S.H.","Jeon Y.J.","Chung C.H."],"citationCrossReferences":[{"database":"PubMed","id":"25219498"},{"database":"DOI","id":"10.1016/j.molcel.2014.08.007"}],"title":"Modification of ASC1 by UFM1 is crucial for ERalpha transactivation and breast cancer development.","publicationDate":"2014","journal":"Mol. Cell","firstPage":"261","lastPage":"274","volume":"56"},"referencePositions":["FUNCTION","INTERACTION WITH DDRGK1","REGION"]},{"referenceNumber":20,"citation":{"id":"25944712","citationType":"journal article","authors":["Vaca Jacome A.S.","Rabilloud T.","Schaeffer-Reiss C.","Rompais M.","Ayoub D.","Lane L.","Bairoch A.","Van Dorsselaer A.","Carapito C."],"citationCrossReferences":[{"database":"PubMed","id":"25944712"},{"database":"DOI","id":"10.1002/pmic.201400617"}],"title":"N-terminome analysis of the human mitochondrial proteome.","publicationDate":"2015","journal":"Proteomics","firstPage":"2519","lastPage":"2524","volume":"15"},"referencePositions":["IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]"]},{"referenceNumber":21,"citation":{"id":"27351204","citationType":"journal article","authors":["DeJesus R.","Moretti F.","McAllister G.","Wang Z.","Bergman P.","Liu S.","Frias E.","Alford J.","Reece-Hoyes J.S.","Lindeman A.","Kelliher J.","Russ C.","Knehr J.","Carbone W.","Beibel M.","Roma G.","Ng A.","Tallarico J.A.","Porter J.A.","Xavier R.J.","Mickanin C.","Murphy L.O.","Hoffman G.R.","Nyfeler B."],"citationCrossReferences":[{"database":"PubMed","id":"27351204"},{"database":"DOI","id":"10.7554/elife.17290"}],"title":"Functional CRISPR screening identifies the ufmylation pathway as a regulator of SQSTM1/p62.","publicationDate":"2016","journal":"Elife","firstPage":"0","lastPage":"0","volume":"5"},"referencePositions":["FUNCTION"]},{"referenceNumber":22,"citation":{"id":"30354401","citationType":"journal article","authors":["Li J.","Yue G.","Ma W.","Zhang A.","Zou J.","Cai Y.","Tang X.","Wang J.","Liu J.","Li H.","Su H."],"citationCrossReferences":[{"database":"PubMed","id":"30354401"},{"database":"DOI","id":"10.1161/circheartfailure.118.004917"}],"title":"Ufm1-specific ligase Ufl1 regulates endoplasmic reticulum homeostasis and protects against heart failure.","publicationDate":"2018","journal":"Circ. Heart Fail.","firstPage":"e004917","lastPage":"e004917","volume":"11"},"referencePositions":["INDUCTION"]},{"referenceNumber":23,"citation":{"id":"30886146","citationType":"journal article","authors":["Qin B.","Yu J.","Nowsheen S.","Wang M.","Tu X.","Liu T.","Li H.","Wang L.","Lou Z."],"citationCrossReferences":[{"database":"PubMed","id":"30886146"},{"database":"DOI","id":"10.1038/s41467-019-09175-0"}],"title":"UFL1 promotes histone H4 ufmylation and ATM activation.","publicationDate":"2019","journal":"Nat. Commun.","firstPage":"1242","lastPage":"1242","volume":"10"},"referencePositions":["FUNCTION","SUBCELLULAR LOCATION","INTERACTION WITH NBN AND UFC1","PHOSPHORYLATION AT SER-462","MUTAGENESIS OF 125-GLU--VAL-127 AND SER-462"]},{"referenceNumber":24,"citation":{"id":"30783677","citationType":"journal article","authors":["Wang Z.","Gong Y.","Peng B.","Shi R.","Fan D.","Zhao H.","Zhu M.","Zhang H.","Lou Z.","Zhou J.","Zhu W.G.","Cong Y.S.","Xu X."],"citationCrossReferences":[{"database":"PubMed","id":"30783677"},{"database":"DOI","id":"10.1093/nar/gkz110"}],"title":"MRE11 UFMylation promotes ATM activation.","publicationDate":"2019","journal":"Nucleic Acids Res.","firstPage":"4124","lastPage":"4135","volume":"47"},"referencePositions":["FUNCTION"]},{"referenceNumber":25,"citation":{"id":"30626644","citationType":"journal article","authors":["Walczak C.P.","Leto D.E.","Zhang L.","Riepe C.","Muller R.Y.","DaRosa P.A.","Ingolia N.T.","Elias J.E.","Kopito R.R."],"citationCrossReferences":[{"database":"PubMed","id":"30626644"},{"database":"DOI","id":"10.1073/pnas.1816202116"}],"title":"Ribosomal protein RPL26 is the principal target of UFMylation.","publicationDate":"2019","journal":"Proc. Natl. Acad. Sci. U.S.A.","firstPage":"1299","lastPage":"1308","volume":"116"},"referencePositions":["FUNCTION"]},{"referenceNumber":26,"citation":{"id":"32160526","citationType":"journal article","authors":["Liang J.R.","Lingeman E.","Luong T.","Ahmed S.","Muhar M.","Nguyen T.","Olzmann J.A.","Corn J.E."],"citationCrossReferences":[{"database":"PubMed","id":"32160526"},{"database":"DOI","id":"10.1016/j.cell.2020.02.017"}],"title":"A genome-wide ER-phagy screen highlights key roles of mitochondrial metabolism and ER-Resident UFMylation.","publicationDate":"2020","journal":"Cell","firstPage":"1160","lastPage":"1177","volume":"180"},"referencePositions":["FUNCTION","SUBCELLULAR LOCATION","INTERACTION WITH DDRGK1"]},{"referenceNumber":27,"citation":{"id":"32050156","citationType":"journal article","authors":["Yang G.","Wang Y.","Chen Y.","Huang R."],"citationCrossReferences":[{"database":"PubMed","id":"32050156"},{"database":"DOI","id":"10.1016/j.intimp.2020.106278"}],"title":"UFL1 attenuates IL-1beta-induced inflammatory response in human osteoarthritis chondrocytes.","publicationDate":"2020","journal":"Int. Immunopharmacol.","firstPage":"106278","lastPage":"106278","volume":"81"},"referencePositions":["FUNCTION"]},{"referenceNumber":28,"citation":{"id":"32807901","citationType":"journal article","authors":["Liu J.","Guan D.","Dong M.","Yang J.","Wei H.","Liang Q.","Song L.","Xu L.","Bai J.","Liu C.","Mao J.","Zhang Q.","Zhou J.","Wu X.","Wang M.","Cong Y.S."],"citationCrossReferences":[{"database":"PubMed","id":"32807901"},{"database":"DOI","id":"10.1038/s41556-020-0559-z"}],"title":"UFMylation maintains tumour suppressor p53 stability by antagonizing its ubiquitination.","publicationDate":"2020","journal":"Nat. Cell Biol.","firstPage":"1056","lastPage":"1063","volume":"22"},"referencePositions":["FUNCTION"]},{"referenceNumber":29,"citation":{"id":"36121123","citationType":"journal article","authors":["Peter J.J.","Magnussen H.M.","DaRosa P.A.","Millrine D.","Matthews S.P.","Lamoliatte F.","Sundaramoorthy R.","Kopito R.R.","Kulathu Y."],"citationCrossReferences":[{"database":"PubMed","id":"36121123"},{"database":"DOI","id":"10.15252/embj.2022111015"}],"title":"A non-canonical scaffold-type E3 ligase complex mediates protein UFMylation.","publicationDate":"2022","journal":"EMBO J.","firstPage":"e111015","lastPage":"e111015","volume":"41"},"referencePositions":["FUNCTION","IDENTIFICATION IN THE UREL COMPLEX","MUTAGENESIS OF CYS-32; CYS-143; CYS-300 AND CYS-372"]},{"referenceNumber":30,"citation":{"id":"35753586","citationType":"journal article","authors":["Zhu J.","Ma X.","Jing Y.","Zhang G.","Zhang D.","Mao Z.","Ma X.","Liu H.","Chen F."],"citationCrossReferences":[{"database":"PubMed","id":"35753586"},{"database":"DOI","id":"10.1016/j.freeradbiomed.2022.06.237"}],"title":"P4HB UFMylation regulates mitochondrial function and oxidative stress.","publicationDate":"2022","journal":"Free Radic. Biol. Med.","firstPage":"277","lastPage":"286","volume":"188"},"referencePositions":["FUNCTION"]},{"referenceNumber":31,"citation":{"id":"36543799","citationType":"journal article","authors":["Ishimura R.","El-Gowily A.H.","Noshiro D.","Komatsu-Hirota S.","Ono Y.","Shindo M.","Hatta T.","Abe M.","Uemura T.","Lee-Okada H.C.","Mohamed T.M.","Yokomizo T.","Ueno T.","Sakimura K.","Natsume T.","Sorimachi H.","Inada T.","Waguri S.","Noda N.N.","Komatsu M."],"citationCrossReferences":[{"database":"PubMed","id":"36543799"},{"database":"DOI","id":"10.1038/s41467-022-35501-0"}],"title":"The UFM1 system regulates ER-phagy through the ufmylation of CYB5R3.","publicationDate":"2022","journal":"Nat. Commun.","firstPage":"7857","lastPage":"7857","volume":"13"},"referencePositions":["FUNCTION","SUBCELLULAR LOCATION","IDENTIFICATION IN THE UREL COMPLEX"]},{"referenceNumber":32,"citation":{"id":"35394863","citationType":"journal article","authors":["Snider D.L.","Park M.","Murphy K.A.","Beachboard D.C.","Horner S.M."],"citationCrossReferences":[{"database":"PubMed","id":"35394863"},{"database":"DOI","id":"10.1073/pnas.2119531119"}],"title":"Signaling from the RNA sensor RIG-I is regulated by ufmylation.","publicationDate":"2022","journal":"Proc. Natl. Acad. Sci. U.S.A.","firstPage":"e2119531119","lastPage":"e2119531119","volume":"119"},"referencePositions":["FUNCTION"]},{"referenceNumber":33,"citation":{"id":"37311461","citationType":"journal article","authors":["Yiu S.P.T.","Zerbe C.","Vanderwall D.","Huttlin E.L.","Weekes M.P.","Gewurz B.E."],"citationCrossReferences":[{"database":"PubMed","id":"37311461"},{"database":"DOI","id":"10.1016/j.molcel.2023.05.018"}],"title":"An Epstein-Barr virus protein interaction map reveals NLRP3 inflammasome evasion via MAVS UFMylation.","publicationDate":"2023","journal":"Mol. Cell","firstPage":"0","lastPage":"0","volume":"0"},"referencePositions":["FUNCTION","INTERACTION WITH EPSTEIN-BARR VIRUS PROTEIN BILF1 (MICROBIAL INFECTION)"]},{"referenceNumber":34,"citation":{"id":"37795761","citationType":"journal article","authors":["Luo H.","Jiao Q.B.","Shen C.B.","Gong W.Y.","Yuan J.H.","Liu Y.Y.","Chen Z.","Liu J.","Xu X.L.","Cong Y.S.","Zhang X.W."],"citationCrossReferences":[{"database":"PubMed","id":"37795761"},{"database":"DOI","id":"10.1096/fj.202300004rrrr"}],"title":"UFMylation of HRD1 regulates endoplasmic reticulum homeostasis.","publicationDate":"2023","journal":"FASEB J.","firstPage":"e23221","lastPage":"e23221","volume":"37"},"referencePositions":["FUNCTION"]},{"referenceNumber":35,"citation":{"id":"36893266","citationType":"journal article","authors":["Zhou J.","Ma X.","He X.","Chen B.","Yuan J.","Jin Z.","Li L.","Wang Z.","Xiao Q.","Cai Y.","Zou Y.","Cong Y.S."],"citationCrossReferences":[{"database":"PubMed","id":"36893266"},{"database":"DOI","id":"10.1073/pnas.2215732120"}],"title":"Dysregulation of PD-L1 by UFMylation imparts tumor immune evasion and identified as a potential therapeutic target.","publicationDate":"2023","journal":"Proc. Natl. Acad. Sci. U.S.A.","firstPage":"e2215732120","lastPage":"e2215732120","volume":"120"},"referencePositions":["FUNCTION"]},{"referenceNumber":36,"citation":{"id":"37036982","citationType":"journal article","authors":["Scavone F.","Gumbin S.C.","Da Rosa P.A.","Kopito R.R."],"citationCrossReferences":[{"database":"PubMed","id":"37036982"},{"database":"DOI","id":"10.1073/pnas.2220340120"}],"title":"RPL26/uL24 UFMylation is essential for ribosome-associated quality control at the endoplasmic reticulum.","publicationDate":"2023","journal":"Proc. Natl. Acad. Sci. U.S.A.","firstPage":"e2220340120","lastPage":"e2220340120","volume":"120"},"referencePositions":["FUNCTION"]},{"referenceNumber":37,"citation":{"id":"37595036","citationType":"journal article","authors":["Ishimura R.","Ito S.","Mao G.","Komatsu-Hirota S.","Inada T.","Noda N.N.","Komatsu M."],"citationCrossReferences":[{"database":"PubMed","id":"37595036"},{"database":"DOI","id":"10.1126/sciadv.adh3635"}],"title":"Mechanistic insights into the roles of the UFM1 E3 ligase complex in ufmylation and ribosome-associated protein quality control.","publicationDate":"2023","journal":"Sci. Adv.","firstPage":"eadh3635","lastPage":"eadh3635","volume":"9"},"referencePositions":["FUNCTION","IDENTIFICATION IN THE UREL COMPLEX","MUTAGENESIS OF LEU-27; 32-CYS--LEU-39; ASP-129; ARG-185; ARG-191; ARG-199 AND ARG-251"]},{"referenceNumber":38,"citation":{"id":"38377992","citationType":"journal article","authors":["He C.","Xing X.","Chen H.Y.","Gao M.","Shi J.","Xiang B.","Xiao X.","Sun Y.","Yu H.","Xu G.","Yao Y.","Xie Z.","Xing Y.","Budiarto B.R.","Chen S.Y.","Gao Y.","Lee Y.R.","Zhang J."],"citationCrossReferences":[{"database":"PubMed","id":"38377992"},{"database":"DOI","id":"10.1016/j.molcel.2024.01.024"}],"title":"UFL1 ablation in T cells suppresses PD-1 UFMylation to enhance anti-tumor immunity.","publicationDate":"2024","journal":"Mol. Cell","firstPage":"0","lastPage":"0","volume":"0"},"referencePositions":["FUNCTION","INTERACTION WITH YWHAG","PHOSPHORYLATION AT THR-536","MUTAGENESIS OF THR-536"]},{"referenceNumber":39,"citation":{"id":"37988244","citationType":"journal article","authors":["Banerjee S.","Varga J.K.","Kumar M.","Zoltsman G.","Rotem-Bamberger S.","Cohen-Kfir E.","Isupov M.N.","Rosenzweig R.","Schueler-Furman O.","Wiener R."],"citationCrossReferences":[{"database":"PubMed","id":"37988244"},{"database":"DOI","id":"10.15252/embr.202356920"}],"title":"Structural study of UFL1-UFC1 interaction uncovers the role of UFL1 N-terminal helix in ufmylation.","publicationDate":"2023","journal":"EMBO Rep.","firstPage":"e56920","lastPage":"e56920","volume":"24"},"referencePositions":["X-RAY CRYSTALLOGRAPHY (3.07 ANGSTROMS) OF 27-200 IN COMPLEX WITH DDRGK1","INTERACTION WITH UFC1","MUTAGENESIS OF 11-LEU--PHE-15"],"evidences":[{"evidenceCode":"ECO:0007744","source":"PDB","id":"8B9X"}]},{"referenceNumber":40,"citation":{"id":"38383789","citationType":"journal article","authors":["Makhlouf L.","Peter J.J.","Magnussen H.M.","Thakur R.","Millrine D.","Minshull T.C.","Harrison G.","Varghese J.","Lamoliatte F.","Foglizzo M.","Macartney T.","Calabrese A.N.","Zeqiraj E.","Kulathu Y."],"citationCrossReferences":[{"database":"PubMed","id":"38383789"},{"database":"DOI","id":"10.1038/s41586-024-07093-w"}],"title":"The UFM1 E3 ligase recognizes and releases 60S ribosomes from ER translocons.","publicationDate":"2024","journal":"Nature","firstPage":"437","lastPage":"444","volume":"627"},"referencePositions":["STRUCTURE BY ELECTRON MICROSCOPY (3.2 ANGSTROMS) IN COMPLEX WITH CDK5RAP3 AND RIBOSOME","X-RAY CRYSTALLOGRAPHY (2.56 ANGSTROMS) OF 1-179 IN COMPLEX WITH DDRGK1 AND UFC1","FUNCTION","SUBCELLULAR LOCATION","IDENTIFICATION IN THE UREL COMPLEX","INTERACTION WITH UFC1","MUTAGENESIS OF ILE-8; PHE-15 AND GLN-19"],"evidences":[{"evidenceCode":"ECO:0007744","source":"PDB","id":"8C0D"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"8QFC"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"8QFD"}]},{"referenceNumber":41,"citation":{"id":"38383785","citationType":"journal article","authors":["DaRosa P.A.","Penchev I.","Gumbin S.C.","Scavone F.","Wachalska M.","Paulo J.A.","Ordureau A.","Peter J.J.","Kulathu Y.","Harper J.W.","Becker T.","Beckmann R.","Kopito R.R."],"citationCrossReferences":[{"database":"PubMed","id":"38383785"},{"database":"DOI","id":"10.1038/s41586-024-07073-0"}],"title":"UFM1 E3 ligase promotes recycling of 60S ribosomal subunits from the ER.","publicationDate":"2024","journal":"Nature","firstPage":"445","lastPage":"452","volume":"627"},"referencePositions":["STRUCTURE BY ELECTRON MICROSCOPY (2.9 ANGSTROMS) OF THE UREL COMPLEX IN COMPLEX WITH THE 60S RIBOSOME","FUNCTION","SUBCELLULAR LOCATION","IDENTIFICATION IN THE UREL COMPLEX"],"evidences":[{"evidenceCode":"ECO:0007744","source":"PDB","id":"8OJ0"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"8OJ8"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AB018319","properties":[{"key":"ProteinId","value":"BAA34496.1"},{"key":"Status","value":"ALT_INIT"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AK295934","properties":[{"key":"ProteinId","value":"BAG58719.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AL132776","properties":[{"key":"ProteinId","value":"-"},{"key":"Status","value":"NOT_ANNOTATED_CDS"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AL590404","properties":[{"key":"ProteinId","value":"-"},{"key":"Status","value":"NOT_ANNOTATED_CDS"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"CH471051","properties":[{"key":"ProteinId","value":"EAW48507.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"BC015377","properties":[{"key":"ProteinId","value":"AAH15377.1"},{"key":"Status","value":"ALT_SEQ"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"BC028608","properties":[{"key":"ProteinId","value":"AAH28608.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"BC036379","properties":[{"key":"ProteinId","value":"AAH36379.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"CCDS","id":"CCDS5034.1","properties":[{"key":"Description","value":"-"}],"isoformId":"O94874-1"},{"database":"RefSeq","id":"NP_056138.1","properties":[{"key":"NucleotideSequenceId","value":"NM_015323.5"}],"isoformId":"O94874-1"},{"database":"PDB","id":"8B9X","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"3.07 A"},{"key":"Chains","value":"A/B=27-200"}]},{"database":"PDB","id":"8C0D","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.56 A"},{"key":"Chains","value":"A/D=1-179"}]},{"database":"PDB","id":"8OHD","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.10 A"},{"key":"Chains","value":"A=1-794"}]},{"database":"PDB","id":"8OJ0","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.30 A"},{"key":"Chains","value":"A=1-794"}]},{"database":"PDB","id":"8OJ5","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"2.90 A"},{"key":"Chains","value":"A=1-794"}]},{"database":"PDB","id":"8OJ8","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.30 A"},{"key":"Chains","value":"A=1-794"}]},{"database":"PDB","id":"8QFC","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.20 A"},{"key":"Chains","value":"B=1-794"}]},{"database":"PDB","id":"8QFD","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"2.20 A"},{"key":"Chains","value":"s=1-794"}]},{"database":"PDB","id":"9GY4","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.00 A"},{"key":"Chains","value":"E=1-794"}]},{"database":"PDBsum","id":"8B9X","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"8C0D","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"8OHD","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"8OJ0","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"8OJ5","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"8OJ8","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"8QFC","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"8QFD","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"9GY4","properties":[{"key":"Description","value":"-"}]},{"database":"AlphaFoldDB","id":"O94874","properties":[{"key":"Description","value":"-"}]},{"database":"EMDB","id":"EMD-16880","properties":[{"key":"Description","value":"-"}]},{"database":"EMDB","id":"EMD-16902","properties":[{"key":"Description","value":"-"}]},{"database":"EMDB","id":"EMD-16905","properties":[{"key":"Description","value":"-"}]},{"database":"EMDB","id":"EMD-16908","properties":[{"key":"Description","value":"-"}]},{"database":"EMDB","id":"EMD-18381","properties":[{"key":"Description","value":"-"}]},{"database":"EMDB","id":"EMD-18382","properties":[{"key":"Description","value":"-"}]},{"database":"EMDB","id":"EMD-51681","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"O94874","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"116953","properties":[{"key":"Interactions","value":"1088"}]},{"database":"ComplexPortal","id":"CPX-8304","properties":[{"key":"EntryName","value":"UFM1 ribosome E3 ligase complex"}]},{"database":"FunCoup","id":"O94874","properties":[{"key":"Number of interactors","value":"3588"}]},{"database":"IntAct","id":"O94874","properties":[{"key":"Interactions","value":"106"}]},{"database":"MINT","id":"O94874","properties":[{"key":"Description","value":"-"}]},{"database":"NDEx","id":"IQUERY-CP-UFL1","properties":[{"key":"Evidences","value":"1 NDEx IQuery Curated Pathway"}]},{"database":"STRING","id":"9606.ENSP00000358283","properties":[{"key":"Description","value":"-"}]},{"database":"BindingDB","id":"O94874","properties":[{"key":"Description","value":"-"}]},{"database":"ChEMBL","id":"CHEMBL6066996","properties":[{"key":"Description","value":"-"}]},{"database":"GlyGen","id":"O94874","properties":[{"key":"glycosylation","value":"2 sites, 1 O-linked glycan (1 site)"}]},{"database":"iPTMnet","id":"O94874","properties":[{"key":"Description","value":"-"}]},{"database":"MetOSite","id":"O94874","properties":[{"key":"Description","value":"-"}]},{"database":"PhosphoSitePlus","id":"O94874","properties":[{"key":"Description","value":"-"}]},{"database":"SwissPalm","id":"O94874","properties":[{"key":"Description","value":"-"}]},{"database":"BioMuta","id":"UFL1","properties":[{"key":"Description","value":"-"}]},{"database":"jPOST","id":"O94874","properties":[{"key":"Description","value":"-"}]},{"database":"MassIVE","id":"O94874","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"9606-ENSP00000358283","properties":[{"key":"Description","value":"-"}]},{"database":"PeptideAtlas","id":"O94874","properties":[{"key":"Description","value":"-"}]},{"database":"ProteomicsDB","id":"50509","properties":[{"key":"Description","value":"-"}],"isoformId":"O94874-1"},{"database":"ProteomicsDB","id":"50510","properties":[{"key":"Description","value":"-"}],"isoformId":"O94874-2"},{"database":"ProteomicsDB","id":"50511","properties":[{"key":"Description","value":"-"}],"isoformId":"O94874-3"},{"database":"Pumba","id":"O94874","properties":[{"key":"Description","value":"-"}]},{"database":"Antibodypedia","id":"31948","properties":[{"key":"antibodies","value":"111 antibodies from 24 providers"}]},{"database":"DNASU","id":"23376","properties":[{"key":"Description","value":"-"}]},{"database":"Ensembl","id":"ENST00000369278.5","properties":[{"key":"ProteinId","value":"ENSP00000358283.4"},{"key":"GeneId","value":"ENSG00000014123.11"}],"isoformId":"O94874-1"},{"database":"GeneID","id":"23376","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"hsa:23376","properties":[{"key":"Description","value":"-"}]},{"database":"MANE-Select","id":"ENST00000369278.5","properties":[{"key":"ProteinId","value":"ENSP00000358283.4"},{"key":"RefSeqNucleotideId","value":"NM_015323.5"},{"key":"RefSeqProteinId","value":"NP_056138.1"}]},{"database":"UCSC","id":"uc003por.4","properties":[{"key":"OrganismName","value":"human"}],"isoformId":"O94874-1"},{"database":"AGR","id":"HGNC:23039","properties":[{"key":"Description","value":"-"}]},{"database":"ClinPGx","id":"PA134937763","properties":[{"key":"Description","value":"-"}]},{"database":"CTD","id":"23376","properties":[{"key":"Description","value":"-"}]},{"database":"DisGeNET","id":"23376","properties":[{"key":"Description","value":"-"}]},{"database":"GeneCards","id":"UFL1","properties":[{"key":"Description","value":"-"}]},{"database":"HGNC","id":"HGNC:23039","properties":[{"key":"GeneName","value":"UFL1"}]},{"database":"HPA","id":"ENSG00000014123","properties":[{"key":"ExpressionPatterns","value":"Low tissue specificity"}]},{"database":"MIM","id":"613372","properties":[{"key":"Type","value":"gene"}]},{"database":"OpenTargets","id":"ENSG00000014123","properties":[{"key":"Description","value":"-"}]},{"database":"VEuPathDB","id":"HostDB:ENSG00000014123","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"KOG2235","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"GeneTree","id":"ENSGT00390000002112","properties":[{"key":"Description","value":"-"}]},{"database":"HOGENOM","id":"CLU_012417_1_0_1","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"O94874","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"CILHASG","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"10258297at2759","properties":[{"key":"Description","value":"-"}]},{"database":"PAN-GO","id":"O94874","properties":[{"key":"Number of GO annotations","value":"4 GO annotations based on evolutionary models"}]},{"database":"PhylomeDB","id":"O94874","properties":[{"key":"Description","value":"-"}]},{"database":"PathwayCommons","id":"O94874","properties":[{"key":"Description","value":"-"}]},{"database":"Reactome","id":"R-HSA-983168","properties":[{"key":"PathwayName","value":"Antigen processing: Ubiquitination & Proteasome degradation"}]},{"database":"SignaLink","id":"O94874","properties":[{"key":"Description","value":"-"}]},{"database":"SIGNOR","id":"O94874","properties":[{"key":"Description","value":"-"}]},{"database":"Agora","id":"ENSG00000014123","properties":[{"key":"NominatedTarget","value":"-"}]},{"database":"BioGRID-ORCS","id":"23376","properties":[{"key":"hits","value":"295 hits in 1174 CRISPR screens"}]},{"database":"CD-CODE","id":"FB4E32DD","properties":[{"key":"EntryName","value":"Presynaptic clusters and postsynaptic densities"}]},{"database":"ChiTaRS","id":"UFL1","properties":[{"key":"OrganismName","value":"human"}]},{"database":"GenomeRNAi","id":"23376","properties":[{"key":"Description","value":"-"}]},{"database":"Pharos","id":"O94874","properties":[{"key":"DevelopmentLevel","value":"Tbio"}]},{"database":"PRO","id":"PR:O94874","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000005640","properties":[{"key":"Component","value":"Chromosome 6"}]},{"database":"RNAct","id":"O94874","properties":[{"key":"moleculeType","value":"protein"}]},{"database":"Bgee","id":"ENSG00000014123","properties":[{"key":"ExpressionPatterns","value":"Expressed in caput epididymis and 212 other cell types or tissues"}]},{"database":"GO","id":"GO:0005694","properties":[{"key":"GoTerm","value":"C:chromosome"},{"key":"GoEvidenceType","value":"EXP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30886146"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"20164180"},{"evidenceCode":"ECO:0000314","source":"PubMed","id":"30886146"}]},{"database":"GO","id":"GO:0005829","properties":[{"key":"GoTerm","value":"C:cytosol"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0005783","properties":[{"key":"GoTerm","value":"C:endoplasmic reticulum"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"20018847"},{"evidenceCode":"ECO:0000314","source":"PubMed","id":"37795761"}]},{"database":"GO","id":"GO:0005789","properties":[{"key":"GoTerm","value":"C:endoplasmic reticulum membrane"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"32160526"},{"evidenceCode":"ECO:0000314","source":"PubMed","id":"36543799"},{"evidenceCode":"ECO:0000314","source":"PubMed","id":"38383785"},{"evidenceCode":"ECO:0000314","source":"PubMed","id":"38383789"}]},{"database":"GO","id":"GO:0016020","properties":[{"key":"GoTerm","value":"C:membrane"},{"key":"GoEvidenceType","value":"HDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0007005","source":"PubMed","id":"16210410"}]},{"database":"GO","id":"GO:0005741","properties":[{"key":"GoTerm","value":"C:mitochondrial outer membrane"},{"key":"GoEvidenceType","value":"IDA:UniProt"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"20164180"}]},{"database":"GO","id":"GO:0043005","properties":[{"key":"GoTerm","value":"C:neuron projection"},{"key":"GoEvidenceType","value":"IEA:Ensembl"}]},{"database":"GO","id":"GO:0005634","properties":[{"key":"GoTerm","value":"C:nucleus"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"30886146"}]},{"database":"GO","id":"GO:0032991","properties":[{"key":"GoTerm","value":"C:protein-containing complex"},{"key":"GoEvidenceType","value":"IDA:MGI"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"20531390"}]},{"database":"GO","id":"GO:0035861","properties":[{"key":"GoTerm","value":"C:site of double-strand break"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"30886146"}]},{"database":"GO","id":"GO:1990234","properties":[{"key":"GoTerm","value":"C:transferase complex"},{"key":"GoEvidenceType","value":"IPI:ComplexPortal"}],"evidences":[{"evidenceCode":"ECO:0000353","source":"PubMed","id":"36121123"}]},{"database":"GO","id":"GO:0019901","properties":[{"key":"GoTerm","value":"F:protein kinase binding"},{"key":"GoEvidenceType","value":"IPI:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000353","source":"PubMed","id":"30886146"}]},{"database":"GO","id":"GO:0061666","properties":[{"key":"GoTerm","value":"F:UFM1 ligase activity"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"20018847"},{"evidenceCode":"ECO:0000314","source":"PubMed","id":"30626644"},{"evidenceCode":"ECO:0000314","source":"PubMed","id":"30783677"},{"evidenceCode":"ECO:0000314","source":"PubMed","id":"30886146"},{"evidenceCode":"ECO:0000314","source":"PubMed","id":"32160526"},{"evidenceCode":"ECO:0000314","source":"PubMed","id":"32807901"},{"evidenceCode":"ECO:0000314","source":"PubMed","id":"35753586"},{"evidenceCode":"ECO:0000314","source":"PubMed","id":"36121123"},{"evidenceCode":"ECO:0000314","source":"PubMed","id":"36543799"},{"evidenceCode":"ECO:0000314","source":"PubMed","id":"36893266"},{"evidenceCode":"ECO:0000314","source":"PubMed","id":"37595036"},{"evidenceCode":"ECO:0000314","source":"PubMed","id":"37795761"},{"evidenceCode":"ECO:0000314","source":"PubMed","id":"38377992"},{"evidenceCode":"ECO:0000314","source":"PubMed","id":"38383785"},{"evidenceCode":"ECO:0000314","source":"PubMed","id":"38383789"}]},{"database":"GO","id":"GO:0071568","properties":[{"key":"GoTerm","value":"F:UFM1 transferase activity"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0000077","properties":[{"key":"GoTerm","value":"P:DNA damage checkpoint signaling"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"30886146"}]},{"database":"GO","id":"GO:0006974","properties":[{"key":"GoTerm","value":"P:DNA damage response"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"30783677"},{"evidenceCode":"ECO:0000314","source":"PubMed","id":"32807901"}]},{"database":"GO","id":"GO:0030218","properties":[{"key":"GoTerm","value":"P:erythrocyte differentiation"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0060218","properties":[{"key":"GoTerm","value":"P:hematopoietic stem cell differentiation"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:1903895","properties":[{"key":"GoTerm","value":"P:negative regulation of IRE1-mediated unfolded protein response"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"32160526"}]},{"database":"GO","id":"GO:0031397","properties":[{"key":"GoTerm","value":"P:negative regulation of protein ubiquitination"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"20164180"}]},{"database":"GO","id":"GO:0050868","properties":[{"key":"GoTerm","value":"P:negative regulation of T cell activation"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"38377992"}]},{"database":"GO","id":"GO:0002841","properties":[{"key":"GoTerm","value":"P:negative regulation of T cell mediated immune response to tumor cell"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"38377992"}]},{"database":"GO","id":"GO:0001649","properties":[{"key":"GoTerm","value":"P:osteoblast differentiation"},{"key":"GoEvidenceType","value":"HDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0007005","source":"PubMed","id":"16210410"}]},{"database":"GO","id":"GO:0010508","properties":[{"key":"GoTerm","value":"P:positive regulation of autophagy"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0008284","properties":[{"key":"GoTerm","value":"P:positive regulation of cell population proliferation"},{"key":"GoEvidenceType","value":"IMP:MGI"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"20531390"}]},{"database":"GO","id":"GO:0045732","properties":[{"key":"GoTerm","value":"P:positive regulation of protein catabolic process"},{"key":"GoEvidenceType","value":"IDA:UniProt"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"36543799"}]},{"database":"GO","id":"GO:0140501","properties":[{"key":"GoTerm","value":"P:positive regulation of reticulophagy"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"36543799"}]},{"database":"GO","id":"GO:1990592","properties":[{"key":"GoTerm","value":"P:protein K69-linked ufmylation"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"25219498"}]},{"database":"GO","id":"GO:0050821","properties":[{"key":"GoTerm","value":"P:protein stabilization"},{"key":"GoEvidenceType","value":"IDA:UniProt"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"32807901"},{"evidenceCode":"ECO:0000314","source":"PubMed","id":"38377992"}]},{"database":"GO","id":"GO:0071569","properties":[{"key":"GoTerm","value":"P:protein ufmylation"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"20018847"},{"evidenceCode":"ECO:0000314","source":"PubMed","id":"30626644"},{"evidenceCode":"ECO:0000314","source":"PubMed","id":"30886146"},{"evidenceCode":"ECO:0000314","source":"PubMed","id":"32160526"},{"evidenceCode":"ECO:0000314","source":"PubMed","id":"35753586"},{"evidenceCode":"ECO:0000314","source":"PubMed","id":"36121123"},{"evidenceCode":"ECO:0000314","source":"PubMed","id":"36543799"},{"evidenceCode":"ECO:0000314","source":"PubMed","id":"37595036"},{"evidenceCode":"ECO:0000314","source":"PubMed","id":"37795761"},{"evidenceCode":"ECO:0000314","source":"PubMed","id":"38383785"},{"evidenceCode":"ECO:0000314","source":"PubMed","id":"38383789"}]},{"database":"GO","id":"GO:0043122","properties":[{"key":"GoTerm","value":"P:regulation of canonical NF-kappaB signal transduction"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0050727","properties":[{"key":"GoTerm","value":"P:regulation of inflammatory response"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0033146","properties":[{"key":"GoTerm","value":"P:regulation of intracellular estrogen receptor signaling pathway"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"25219498"}]},{"database":"GO","id":"GO:0032434","properties":[{"key":"GoTerm","value":"P:regulation of proteasomal ubiquitin-dependent protein catabolic process"},{"key":"GoEvidenceType","value":"IMP:MGI"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"20228063"}]},{"database":"GO","id":"GO:0032880","properties":[{"key":"GoTerm","value":"P:regulation of protein localization"},{"key":"GoEvidenceType","value":"IMP:MGI"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"20531390"}]},{"database":"GO","id":"GO:0072344","properties":[{"key":"GoTerm","value":"P:rescue of stalled cytosolic ribosome"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"37595036"},{"evidenceCode":"ECO:0000314","source":"PubMed","id":"38383785"},{"evidenceCode":"ECO:0000314","source":"PubMed","id":"38383789"}]},{"database":"GO","id":"GO:0034976","properties":[{"key":"GoTerm","value":"P:response to endoplasmic reticulum stress"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"32160526"}]},{"database":"GO","id":"GO:0061709","properties":[{"key":"GoTerm","value":"P:reticulophagy"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"32160526"}]},{"database":"GO","id":"GO:0032790","properties":[{"key":"GoTerm","value":"P:ribosome disassembly"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"38383785"},{"evidenceCode":"ECO:0000314","source":"PubMed","id":"38383789"}]},{"database":"InterPro","id":"IPR018611","properties":[{"key":"EntryName","value":"Ufl1"}]},{"database":"InterPro","id":"IPR056761","properties":[{"key":"EntryName","value":"Ufl1-like_C"}]},{"database":"InterPro","id":"IPR056580","properties":[{"key":"EntryName","value":"Ufl1_dom"}]},{"database":"InterPro","id":"IPR056579","properties":[{"key":"EntryName","value":"Ufl1_N"}]},{"database":"PANTHER","id":"PTHR31057","properties":[{"key":"EntryName","value":"E3 UFM1-PROTEIN LIGASE 1"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR31057:SF0","properties":[{"key":"EntryName","value":"E3 UFM1-PROTEIN LIGASE 1"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF09743","properties":[{"key":"EntryName","value":"E3_UFM1_ligase"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF23659","properties":[{"key":"EntryName","value":"UFL1"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF25041","properties":[{"key":"EntryName","value":"UFL1_C"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF25870","properties":[{"key":"EntryName","value":"WHD_UFL1_5th"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MADAWEEIRRLAADFQRAQFAEATQRLSERNCIEIVNKLIAQKQLEVVHTLDGKEYITPAQISKEMRDELHVRGGRVNIVDLQQVINVDLIHIENRIGDIIKSEKHVQLVLGQLIDENYLDRLAEEVNDKLQESGQVTISELCKTYDLPGNFLTQALTQRLGRIISGHIDLDNRGVIFTEAFVARHKARIRGLFSAITRPTAVNSLISKYGFQEQLLYSVLEELVNSGRLRGTVVGGRQDKAVFVPDIYSRTQSTWVDSFFRQNGYLEFDALSRLGIPDAVSYIKKRYKTTQLLFLKAACVGQGLVDQVEASVEEAISSGTWVDIAPLLPTSLSVEDAAILLQQVMRAFSKQASTVVFSDTVVVSEKFINDCTELFRELMHQKAEKEMKNNPVHLITEEDLKQISTLESVSTSKKDKKDERRRKATEGSGSMRGGGGGNAREYKIKKVKKKGRKDDDSDDESQSSHTGKKKPEISFMFQDEIEDFLRKHIQDAPEEFISELAEYLIKPLNKTYLEVVRSVFMSSTTSASGTGRKRTIKDLQEEVSNLYNNIRLFEKGMKFFADDTQAALTKHLLKSVCTDITNLIFNFLASDLMMAVDDPAAITSEIRKKILSKLSEETKVALTKLHNSLNEKSIEDFISCLDSAAEACDIMVKRGDKKRERQILFQHRQALAEQLKVTEDPALILHLTSVLLFQFSTHSMLHAPGRCVPQIIAFLNSKIPEDQHALLVKYQGLVVKQLVSQSKKTGQGDYPLNNELDKEQEDVASTTRKELQELSSSIKDLVLKSRKSSVTEE","length":794,"molWeight":89595,"crc64":"C6C1777455551991","md5":"BC18795CB497BD001E05DCAA5C9F5CF3"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBUNIT":2,"INTERACTION":3,"SUBCELLULAR LOCATION":1,"ALTERNATIVE PRODUCTS":3,"TISSUE SPECIFICITY":1,"INDUCTION":1,"PTM":2,"SIMILARITY":1,"SEQUENCE CAUTION":2},"countByFeatureType":{"Initiator methionine":1,"Chain":1,"Region":7,"Modified residue":5,"Alternative sequence":3,"Natural variant":1,"Mutagenesis":19,"Helix":46,"Beta strand":19,"Turn":5},"uniParcId":"UPI0000072D61"}}