{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"O80831","secondaryAccessions":["Q0WU61"],"uniProtkbId":"BAM7_ARATH","entryAudit":{"firstPublicDate":"2010-04-20","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2010-04-20","entryVersion":146,"sequenceVersion":2},"annotationScore":4.0,"organism":{"scientificName":"Arabidopsis thaliana","commonName":"Mouse-ear cress","taxonId":3702,"lineage":["Eukaryota","Viridiplantae","Streptophyta","Embryophyta","Tracheophyta","Spermatophyta","Magnoliopsida","eudicotyledons","Gunneridae","Pentapetalae","rosids","malvids","Brassicales","Brassicaceae","Camelineae","Arabidopsis"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Beta-amylase 7"},"ecNumbers":[{"value":"3.2.1.2"}]},"alternativeNames":[{"fullName":{"value":"1,4-alpha-D-glucan maltohydrolase"}},{"fullName":{"value":"Beta-amylase 4"}}]},"genes":[{"geneName":{"value":"BAM7"},"synonyms":[{"value":"BMY4"}],"orderedLocusNames":[{"value":"At2g45880"}],"orfNames":[{"value":"F4I18.14"}]}],"comments":[{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"Hydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides so as to remove successive maltose units from the non-reducing ends of the chains.","ecNumber":"3.2.1.2"}},{"commentType":"INTERACTION","interactions":[{"interactantOne":{"uniProtKBAccession":"O80831","intActId":"EBI-15212616"},"interactantTwo":{"uniProtKBAccession":"Q9FH80","geneName":"BAM8","intActId":"EBI-4427212"},"numberOfExperiments":5,"organismDiffer":false}]},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Cytoplasm","id":"SL-0086"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the glycosyl hydrolase 14 family"}],"commentType":"SIMILARITY"},{"commentType":"SEQUENCE CAUTION","sequenceCautionType":"Erroneous gene model prediction","sequence":"AAC28536.1","evidences":[{"evidenceCode":"ECO:0000305"}]}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":691,"modifier":"EXACT"}},"description":"Beta-amylase 7","featureId":"PRO_0000393421"},{"type":"Region","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":83,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":130,"modifier":"EXACT"},"end":{"value":167,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":12,"modifier":"EXACT"},"end":{"value":29,"modifier":"EXACT"}},"description":"Acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":30,"modifier":"EXACT"},"end":{"value":39,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":41,"modifier":"EXACT"},"end":{"value":59,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":71,"modifier":"EXACT"},"end":{"value":80,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":132,"modifier":"EXACT"},"end":{"value":143,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":144,"modifier":"EXACT"},"end":{"value":160,"modifier":"EXACT"}},"description":"Low complexity","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Active site","location":{"start":{"value":422,"modifier":"EXACT"},"end":{"value":422,"modifier":"EXACT"}},"description":"Proton donor","evidences":[{"evidenceCode":"ECO:0000250"}]},{"type":"Active site","location":{"start":{"value":618,"modifier":"EXACT"},"end":{"value":618,"modifier":"EXACT"}},"description":"Proton acceptor","evidences":[{"evidenceCode":"ECO:0000250"}]},{"type":"Binding site","location":{"start":{"value":289,"modifier":"EXACT"},"end":{"value":289,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"substrate"}},{"type":"Binding site","location":{"start":{"value":329,"modifier":"EXACT"},"end":{"value":329,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"substrate"}},{"type":"Binding site","location":{"start":{"value":337,"modifier":"EXACT"},"end":{"value":337,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"substrate"}},{"type":"Binding site","location":{"start":{"value":530,"modifier":"EXACT"},"end":{"value":530,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"substrate"}},{"type":"Binding site","location":{"start":{"value":535,"modifier":"EXACT"},"end":{"value":535,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"substrate"}},{"type":"Binding site","location":{"start":{"value":619,"modifier":"EXACT"},"end":{"value":620,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"substrate"}},{"type":"Binding site","location":{"start":{"value":654,"modifier":"EXACT"},"end":{"value":654,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"substrate"}},{"type":"Modified residue","location":{"start":{"value":195,"modifier":"EXACT"},"end":{"value":195,"modifier":"EXACT"}},"description":"Phosphoserine","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9LIR6"}]},{"type":"Sequence conflict","location":{"start":{"value":377,"modifier":"EXACT"},"end":{"value":377,"modifier":"EXACT"}},"description":"in Ref. 4; BAE99337","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"K","alternativeSequences":["R"]}}],"keywords":[{"id":"KW-0119","category":"Biological process","name":"Carbohydrate metabolism"},{"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"id":"KW-0326","category":"Molecular function","name":"Glycosidase"},{"id":"KW-0378","category":"Molecular function","name":"Hydrolase"},{"id":"KW-0597","category":"PTM","name":"Phosphoprotein"},{"id":"KW-0624","category":"Biological process","name":"Polysaccharide degradation"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"}],"references":[{"referenceNumber":1,"citation":{"id":"10617197","citationType":"journal article","authors":["Lin X.","Kaul S.","Rounsley S.D.","Shea T.P.","Benito M.-I.","Town C.D.","Fujii C.Y.","Mason T.M.","Bowman C.L.","Barnstead M.E.","Feldblyum T.V.","Buell C.R.","Ketchum K.A.","Lee J.J.","Ronning C.M.","Koo H.L.","Moffat K.S.","Cronin L.A.","Shen M.","Pai G.","Van Aken S.","Umayam L.","Tallon L.J.","Gill J.E.","Adams M.D.","Carrera A.J.","Creasy T.H.","Goodman H.M.","Somerville C.R.","Copenhaver G.P.","Preuss D.","Nierman W.C.","White O.","Eisen J.A.","Salzberg S.L.","Fraser C.M.","Venter J.C."],"citationCrossReferences":[{"database":"PubMed","id":"10617197"},{"database":"DOI","id":"10.1038/45471"}],"title":"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.","publicationDate":"1999","journal":"Nature","firstPage":"761","lastPage":"768","volume":"402"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"27862469","citationType":"journal article","authors":["Cheng C.Y.","Krishnakumar V.","Chan A.P.","Thibaud-Nissen F.","Schobel S.","Town C.D."],"citationCrossReferences":[{"database":"PubMed","id":"27862469"},{"database":"DOI","id":"10.1111/tpj.13415"}],"title":"Araport11: a complete reannotation of the Arabidopsis thaliana reference genome.","publicationDate":"2017","journal":"Plant J.","firstPage":"789","lastPage":"804","volume":"89"},"referencePositions":["GENOME REANNOTATION"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"CI-CI4AM1G71UC0S","citationType":"submission","authoringGroup":["The Arabidopsis Information Resource (TAIR)"],"title":"Community-consensus reference genome of Arabidopsis thaliana Col-0.","publicationDate":"FEB-2026","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["GENOME REANNOTATION"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":4,"citation":{"id":"CI-1ND7IVR87IF4I","citationType":"submission","authors":["Totoki Y.","Seki M.","Ishida J.","Nakajima M.","Enju A.","Kamiya A.","Narusaka M.","Shin-i T.","Nakagawa M.","Sakamoto N.","Oishi K.","Kohara Y.","Kobayashi M.","Toyoda A.","Sakaki Y.","Sakurai T.","Iida K.","Akiyama K.","Satou M.","Toyoda T.","Konagaya A.","Carninci P.","Kawai J.","Hayashizaki Y.","Shinozaki K."],"title":"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.","publicationDate":"JUL-2006","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":5,"citation":{"id":"18390594","citationType":"journal article","authors":["Fulton D.C.","Stettler M.","Mettler T.","Vaughan C.K.","Li J.","Francisco P.","Gil M.","Reinhold H.","Eicke S.","Messerli G.","Dorken G.","Halliday K.","Smith A.M.","Smith S.M.","Zeeman S.C."],"citationCrossReferences":[{"database":"PubMed","id":"18390594"},{"database":"DOI","id":"10.1105/tpc.107.056507"}],"title":"Beta-AMYLASE4, a noncatalytic protein required for starch breakdown, acts upstream of three active beta-amylases in Arabidopsis chloroplasts.","publicationDate":"2008","journal":"Plant Cell","firstPage":"1040","lastPage":"1058","volume":"20"},"referencePositions":["GENE FAMILY","NOMENCLATURE"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AC004665","properties":[{"key":"ProteinId","value":"AAC28536.1"},{"key":"Status","value":"ALT_SEQ"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"CP002685","properties":[{"key":"ProteinId","value":"AEC10613.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"OZ408684","properties":[{"key":"ProteinId","value":"CAO7038884.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AK227323","properties":[{"key":"ProteinId","value":"BAE99337.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"PIR","id":"T02459","properties":[{"key":"EntryName","value":"T02459"}]},{"database":"RefSeq","id":"NP_182112.2","properties":[{"key":"NucleotideSequenceId","value":"NM_130151.7"}]},{"database":"AlphaFoldDB","id":"O80831","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"O80831","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"4532","properties":[{"key":"Interactions","value":"2"}]},{"database":"FunCoup","id":"O80831","properties":[{"key":"Number of interactors","value":"1835"}]},{"database":"IntAct","id":"O80831","properties":[{"key":"Interactions","value":"2"}]},{"database":"STRING","id":"3702.O80831","properties":[{"key":"Description","value":"-"}]},{"database":"iPTMnet","id":"O80831","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"3702-O80831","properties":[{"key":"Description","value":"-"}]},{"database":"ProteomicsDB","id":"240636","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"819196","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ath:AT2G45880","properties":[{"key":"Description","value":"-"}]},{"database":"Araport","id":"AT2G45880","properties":[{"key":"Description","value":"-"}]},{"database":"TAIR","id":"AT2G45880","properties":[{"key":"GeneName","value":"BAM7"}]},{"database":"eggNOG","id":"ENOG502QTBX","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"HOGENOM","id":"CLU_016754_4_1_1","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"O80831","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"AVMDHQH","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"1660156at2759","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"O80831","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"ARA:AT2G45880-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"PRO","id":"PR:O80831","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000006548","properties":[{"key":"Component","value":"Chromosome 2"}]},{"database":"ExpressionAtlas","id":"O80831","properties":[{"key":"ExpressionPatterns","value":"baseline and differential"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0005576","properties":[{"key":"GoTerm","value":"C:extracellular region"},{"key":"GoEvidenceType","value":"ISM:TAIR"}]},{"database":"GO","id":"GO:0005634","properties":[{"key":"GoTerm","value":"C:nucleus"},{"key":"GoEvidenceType","value":"IDA:TAIR"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"21487098"}]},{"database":"GO","id":"GO:0003700","properties":[{"key":"GoTerm","value":"F:DNA-binding transcription factor activity"},{"key":"GoEvidenceType","value":"IDA:TAIR"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"21487098"}]},{"database":"GO","id":"GO:0000272","properties":[{"key":"GoTerm","value":"P:polysaccharide catabolic process"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0048831","properties":[{"key":"GoTerm","value":"P:regulation of shoot system development"},{"key":"GoEvidenceType","value":"IMP:TAIR"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"21487098"}]},{"database":"FunFam","id":"3.20.20.80:FF:000066","properties":[{"key":"EntryName","value":"Beta-amylase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.20.20.80","properties":[{"key":"EntryName","value":"Glycosidases"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR008540","properties":[{"key":"EntryName","value":"BES1_N"}]},{"database":"InterPro","id":"IPR017853","properties":[{"key":"EntryName","value":"GH_hydrolase_sf"}]},{"database":"InterPro","id":"IPR001554","properties":[{"key":"EntryName","value":"Glyco_hydro_14"}]},{"database":"InterPro","id":"IPR018238","properties":[{"key":"EntryName","value":"Glyco_hydro_14_CS"}]},{"database":"PANTHER","id":"PTHR31352","properties":[{"key":"EntryName","value":"BETA-AMYLASE 1, CHLOROPLASTIC"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR31352:SF47","properties":[{"key":"EntryName","value":"BETA-AMYLASE 7"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF05687","properties":[{"key":"EntryName","value":"BES1_N"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01373","properties":[{"key":"EntryName","value":"Glyco_hydro_14"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR00750","properties":[{"key":"EntryName","value":"BETAAMYLASE"}]},{"database":"SUPFAM","id":"SSF51445","properties":[{"key":"EntryName","value":"(Trans)glycosidases"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00506","properties":[{"key":"EntryName","value":"BETA_AMYLASE_1"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MATDMHKLLGTSEEDDDEEMDMDVKEEDDGDRRNRDKHAASGSSSNDEFMFQQSMQDQVGTPGGGGSRRSRPLEEKERTKLRERHRRAITARILGGLRRHGNYNLRVRADINDVIAALAREAGWVVLPDGTTFPSKSQGTKPTGGSSAVAAGSSASHIASQQTSPPALRVVSSGLRSPVELSSCRMKGVFTPAPSPYDMLPIQSPELVGSVNKAEGLVGCSVDVINSKQILEIPPNLTEQDFSGTPYVPVYVMLPLGVINMKCELADRDGLLKHLRILKSIHVDGVKVDCWWGIVEGHSPQEYNWTGYRQLFQMVRDLNLKIQVLMSFHECGGNVGDDVCIPLPHWVAEIGRTNPDIYFTDREGRRNPECLSWGIDKERILRGRTALEVYFDYMRSFRIELAEFLEDGVISMVEIGLGPCGELRYPSCPIKHGWRYPGVGEFQCYDKYLSKSLRKAAESRGHLFWARGPDNTGSYNSQPQGTGFFCDGGDYDGLYGRFFLKWYSQVLIDHADQILCLAKLVFDSSCIAAKLPDVHWWYRTASHAAELTAGFYNPSNRDGYSAIASTLKKHGATLSFVSGEVQVLNRPDDFSGALGEPEAVAWQVLNAAWDSGTPVARENSLACHDRVGYNKMLESVKFRNDPDRKHLSSFAYSRLVPALMEGHNIVEFERFVKKLHGEAVMNHHHHHHQQV","length":691,"molWeight":77131,"crc64":"FAB1130D333CACE4","md5":"3D03815D732490D37D2236BBDA641F0F"},"extraAttributes":{"countByCommentType":{"CATALYTIC ACTIVITY":1,"INTERACTION":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1,"SEQUENCE CAUTION":1},"countByFeatureType":{"Chain":1,"Region":2,"Compositional bias":6,"Active site":2,"Binding site":7,"Modified residue":1,"Sequence conflict":1},"uniParcId":"UPI0000196EE1"}}