{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"O76512","secondaryAccessions":["Q9BL16"],"uniProtkbId":"RENT1_CAEEL","entryAudit":{"firstPublicDate":"2001-10-18","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"1998-11-01","entryVersion":163,"sequenceVersion":1},"annotationScore":5.0,"organism":{"scientificName":"Caenorhabditis elegans","taxonId":6239,"lineage":["Eukaryota","Metazoa","Ecdysozoa","Nematoda","Chromadorea","Rhabditida","Rhabditina","Rhabditomorpha","Rhabditoidea","Rhabditidae","Peloderinae","Caenorhabditis"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Regulator of nonsense transcripts 1"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q92900"}],"value":"5.6.2.3"},{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q92900"}],"value":"5.6.2.5"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Double-stranded nucleic acid 5'-3' helicase smg-2"}},{"fullName":{"value":"Nonsense mRNA reducing factor 1"}},{"fullName":{"value":"Up-frameshift suppressor 1 homolog"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"10454541"}],"value":"smg-2"},"orfNames":[{"value":"Y48G8AL.6"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q92900"}],"value":"RNA-dependent helicase required for nonsense-mediated decay (NMD) of aberrant mRNAs containing premature stop codons and modulates the expression level of normal mRNAs. Is recruited to mRNAs upon translation termination and undergoes a cycle of phosphorylation and dephosphorylation; its phosphorylation appears to be a key step in NMD. The formation of an smg-2-3-4 surveillance complex is believed to activate NMD (By similarity)"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"n ATP + n H2O + wound RNA = n ADP + n phosphate + unwound RNA.","ecNumber":"5.6.2.5","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q92900"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"Couples ATP hydrolysis with the unwinding of duplex DNA at the replication fork by translocating in the 5'-3' direction.","ecNumber":"5.6.2.3","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q92900"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"ATP + H2O = ADP + phosphate + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:13065"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:30616"},{"database":"ChEBI","id":"CHEBI:43474"},{"database":"ChEBI","id":"CHEBI:456216"}],"ecNumber":"5.6.2.3","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q92900"}]}},{"commentType":"COFACTOR","cofactors":[{"name":"Mg(2+)","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q92900"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:18420"}}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q92900"}],"value":"Binds 1 Mg(2+) ions per subunit."}]}},{"commentType":"COFACTOR","cofactors":[{"name":"Zn(2+)","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q92900"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:29105"}}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q92900"}],"value":"Bind 3 Zn(2+) ions per subunit."}]}},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"Cytoplasm","id":"SL-0086"}}]},{"texts":[{"value":"Phosphorylated probably by smg-1. Smg-3 and smg-4 are required for phosphorylation"}],"commentType":"PTM"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the DNA2/NAM7 helicase family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1069,"modifier":"EXACT"}},"description":"Regulator of nonsense transcripts 1","featureId":"PRO_0000080719"},{"type":"Domain","location":{"start":{"value":87,"modifier":"EXACT"},"end":{"value":244,"modifier":"EXACT"}},"description":"Upf1 CH-rich","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01341"}]},{"type":"Domain","location":{"start":{"value":445,"modifier":"EXACT"},"end":{"value":672,"modifier":"EXACT"}},"description":"UPF1-type SF1B helicase ATP-binding","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01433"}]},{"type":"Domain","location":{"start":{"value":673,"modifier":"EXACT"},"end":{"value":885,"modifier":"EXACT"}},"description":"UPF1-type SF1B helicase C-terminal","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01433"}]},{"type":"Region","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":86,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":95,"modifier":"EXACT"},"end":{"value":127,"modifier":"EXACT"}},"description":"C3H","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01341"}]},{"type":"Region","location":{"start":{"value":109,"modifier":"EXACT"},"end":{"value":137,"modifier":"EXACT"}},"description":"CC/SHH/C","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01341"}]},{"type":"Region","location":{"start":{"value":155,"modifier":"EXACT"},"end":{"value":185,"modifier":"EXACT"}},"description":"C4","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01341"}]},{"type":"Region","location":{"start":{"value":966,"modifier":"EXACT"},"end":{"value":1069,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":28,"modifier":"EXACT"},"end":{"value":50,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":51,"modifier":"EXACT"},"end":{"value":65,"modifier":"EXACT"}},"description":"Low complexity","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":77,"modifier":"EXACT"},"end":{"value":86,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":991,"modifier":"EXACT"},"end":{"value":1013,"modifier":"EXACT"}},"description":"Low complexity","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Binding site","location":{"start":{"value":95,"modifier":"EXACT"},"end":{"value":95,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29105"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01341"}],"ligand":{"name":"Zn(2+)","id":"ChEBI:CHEBI:29105","label":"1"}},{"type":"Binding site","location":{"start":{"value":98,"modifier":"EXACT"},"end":{"value":98,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29105"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01341"}],"ligand":{"name":"Zn(2+)","id":"ChEBI:CHEBI:29105","label":"1"}},{"type":"Binding site","location":{"start":{"value":109,"modifier":"EXACT"},"end":{"value":109,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29105"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01341"}],"ligand":{"name":"Zn(2+)","id":"ChEBI:CHEBI:29105","label":"2"}},{"type":"Binding site","location":{"start":{"value":112,"modifier":"EXACT"},"end":{"value":112,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29105"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01341"}],"ligand":{"name":"Zn(2+)","id":"ChEBI:CHEBI:29105","label":"2"}},{"type":"Binding site","location":{"start":{"value":117,"modifier":"EXACT"},"end":{"value":117,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29105"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01341"}],"ligand":{"name":"Zn(2+)","id":"ChEBI:CHEBI:29105","label":"1"}},{"type":"Binding site","location":{"start":{"value":127,"modifier":"EXACT"},"end":{"value":127,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29105"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01341"}],"ligand":{"name":"Zn(2+)","id":"ChEBI:CHEBI:29105","label":"1"}},{"type":"Binding site","location":{"start":{"value":131,"modifier":"EXACT"},"end":{"value":131,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29105"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01341"}],"ligand":{"name":"Zn(2+)","id":"ChEBI:CHEBI:29105","label":"2"}},{"type":"Binding site","location":{"start":{"value":137,"modifier":"EXACT"},"end":{"value":137,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29105"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01341"}],"ligand":{"name":"Zn(2+)","id":"ChEBI:CHEBI:29105","label":"2"}},{"type":"Binding site","location":{"start":{"value":155,"modifier":"EXACT"},"end":{"value":155,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29105"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01341"}],"ligand":{"name":"Zn(2+)","id":"ChEBI:CHEBI:29105","label":"3"}},{"type":"Binding site","location":{"start":{"value":158,"modifier":"EXACT"},"end":{"value":158,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29105"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01341"}],"ligand":{"name":"Zn(2+)","id":"ChEBI:CHEBI:29105","label":"3"}},{"type":"Binding site","location":{"start":{"value":181,"modifier":"EXACT"},"end":{"value":181,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29105"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01341"}],"ligand":{"name":"Zn(2+)","id":"ChEBI:CHEBI:29105","label":"3"}},{"type":"Binding site","location":{"start":{"value":185,"modifier":"EXACT"},"end":{"value":185,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29105"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01341"}],"ligand":{"name":"Zn(2+)","id":"ChEBI:CHEBI:29105","label":"3"}},{"type":"Binding site","location":{"start":{"value":444,"modifier":"EXACT"},"end":{"value":444,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q92900"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}},{"type":"Binding site","location":{"start":{"value":445,"modifier":"EXACT"},"end":{"value":445,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q92900"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}},{"type":"Binding site","location":{"start":{"value":470,"modifier":"EXACT"},"end":{"value":470,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q92900"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}},{"type":"Binding site","location":{"start":{"value":471,"modifier":"EXACT"},"end":{"value":471,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q92900"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}},{"type":"Binding site","location":{"start":{"value":472,"modifier":"EXACT"},"end":{"value":472,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q92900"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}},{"type":"Binding site","location":{"start":{"value":473,"modifier":"EXACT"},"end":{"value":473,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q92900"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}},{"type":"Binding site","location":{"start":{"value":474,"modifier":"EXACT"},"end":{"value":474,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q92900"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}},{"type":"Binding site","location":{"start":{"value":474,"modifier":"EXACT"},"end":{"value":474,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q92900"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420"}},{"type":"Binding site","location":{"start":{"value":475,"modifier":"EXACT"},"end":{"value":475,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q92900"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}},{"type":"Binding site","location":{"start":{"value":677,"modifier":"EXACT"},"end":{"value":677,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q92900"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}},{"type":"Binding site","location":{"start":{"value":839,"modifier":"EXACT"},"end":{"value":839,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q92900"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}},{"type":"Mutagenesis","location":{"start":{"value":470,"modifier":"EXACT"},"end":{"value":470,"modifier":"EXACT"}},"description":"In R866; loss of activity and increased phosphorylation.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10454541"}],"alternativeSequence":{"originalSequence":"G","alternativeSequences":["R"]}},{"type":"Mutagenesis","location":{"start":{"value":472,"modifier":"EXACT"},"end":{"value":472,"modifier":"EXACT"}},"description":"In R895; loss of activity and increased phosphorylation.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10454541"}],"alternativeSequence":{"originalSequence":"G","alternativeSequences":["E"]}},{"type":"Sequence conflict","location":{"start":{"value":86,"modifier":"EXACT"},"end":{"value":87,"modifier":"EXACT"}},"description":"in Ref. 2; CCD73461","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"HE","alternativeSequences":["QQ"]}},{"type":"Sequence conflict","location":{"start":{"value":769,"modifier":"EXACT"},"end":{"value":769,"modifier":"EXACT"}},"description":"in Ref. 2; CCD73461","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"S","alternativeSequences":["P"]}}],"keywords":[{"id":"KW-0067","category":"Ligand","name":"ATP-binding"},{"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"id":"KW-0347","category":"Molecular function","name":"Helicase"},{"id":"KW-0378","category":"Molecular function","name":"Hydrolase"},{"id":"KW-0413","category":"Molecular function","name":"Isomerase"},{"id":"KW-0460","category":"Ligand","name":"Magnesium"},{"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"id":"KW-0547","category":"Ligand","name":"Nucleotide-binding"},{"id":"KW-0597","category":"PTM","name":"Phosphoprotein"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0694","category":"Molecular function","name":"RNA-binding"},{"id":"KW-0862","category":"Ligand","name":"Zinc"},{"id":"KW-0863","category":"Domain","name":"Zinc-finger"}],"references":[{"referenceNumber":1,"citation":{"id":"10454541","citationType":"journal article","authors":["Page M.F.","Carr B.","Anders K.R.","Grimson A.","Anderson P."],"citationCrossReferences":[{"database":"PubMed","id":"10454541"},{"database":"DOI","id":"10.1128/mcb.19.9.5943"}],"title":"SMG-2 is a phosphorylated protein required for mRNA surveillance in Caenorhabditis elegans and related to Upf1p of yeast.","publicationDate":"1999","journal":"Mol. Cell. Biol.","firstPage":"5943","lastPage":"5951","volume":"19"},"referencePositions":["NUCLEOTIDE SEQUENCE [MRNA]","MUTAGENESIS OF GLY-470 AND GLY-472"]},{"referenceNumber":2,"citation":{"id":"9851916","citationType":"journal article","authoringGroup":["The C. elegans sequencing consortium"],"citationCrossReferences":[{"database":"PubMed","id":"9851916"},{"database":"DOI","id":"10.1126/science.282.5396.2012"}],"title":"Genome sequence of the nematode C. elegans: a platform for investigating biology.","publicationDate":"1998","journal":"Science","firstPage":"2012","lastPage":"2018","volume":"282"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"Bristol N2","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AF074017","properties":[{"key":"ProteinId","value":"AAC26789.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"FO081800","properties":[{"key":"ProteinId","value":"CCD73461.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"T43280","properties":[{"key":"EntryName","value":"T43280"}]},{"database":"RefSeq","id":"NP_490829.1","properties":[{"key":"NucleotideSequenceId","value":"NM_058428.6"}]},{"database":"AlphaFoldDB","id":"O76512","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"O76512","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"37194","properties":[{"key":"Interactions","value":"28"}]},{"database":"FunCoup","id":"O76512","properties":[{"key":"Number of interactors","value":"3685"}]},{"database":"IntAct","id":"O76512","properties":[{"key":"Interactions","value":"1"}]},{"database":"MINT","id":"O76512","properties":[{"key":"Description","value":"-"}]},{"database":"STRING","id":"6239.Y48G8AL.6.2","properties":[{"key":"Description","value":"-"}]},{"database":"iPTMnet","id":"O76512","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"6239-Y48G8AL.6.2","properties":[{"key":"Description","value":"-"}]},{"database":"PeptideAtlas","id":"O76512","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblMetazoa","id":"Y48G8AL.6.1","properties":[{"key":"ProteinId","value":"Y48G8AL.6.1"},{"key":"GeneId","value":"WBGene00004880"}]},{"database":"GeneID","id":"171696","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"cel:CELE_Y48G8AL.6","properties":[{"key":"Description","value":"-"}]},{"database":"UCSC","id":"Y48G8AL.6","properties":[{"key":"OrganismName","value":"c. elegans"}]},{"database":"AGR","id":"WB:WBGene00004880","properties":[{"key":"Description","value":"-"}]},{"database":"CTD","id":"171696","properties":[{"key":"Description","value":"-"}]},{"database":"WormBase","id":"Y48G8AL.6","properties":[{"key":"ProteinId","value":"CE28367"},{"key":"GeneId","value":"WBGene00004880"},{"key":"GeneName","value":"smg-2"}]},{"database":"eggNOG","id":"KOG1802","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"HOGENOM","id":"CLU_001666_4_1_1","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"O76512","properties":[{"key":"Description","value":"-"}]},{"database":"OrthoDB","id":"6513042at2759","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"O76512","properties":[{"key":"Description","value":"-"}]},{"database":"Reactome","id":"R-CEL-975956","properties":[{"key":"PathwayName","value":"Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC)"}]},{"database":"Reactome","id":"R-CEL-975957","properties":[{"key":"PathwayName","value":"Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC)"}]},{"database":"PRO","id":"PR:O76512","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000001940","properties":[{"key":"Component","value":"Chromosome I"}]},{"database":"Bgee","id":"WBGene00004880","properties":[{"key":"ExpressionPatterns","value":"Expressed in germ line (C elegans) and 4 other cell types or tissues"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"IDA:WormBase"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"16914455"}]},{"database":"GO","id":"GO:0005634","properties":[{"key":"GoTerm","value":"C:nucleus"},{"key":"GoEvidenceType","value":"IDA:WormBase"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"16914455"}]},{"database":"GO","id":"GO:0043139","properties":[{"key":"GoTerm","value":"F:5'-3' DNA helicase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0032574","properties":[{"key":"GoTerm","value":"F:5'-3' RNA helicase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0005524","properties":[{"key":"GoTerm","value":"F:ATP binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0016887","properties":[{"key":"GoTerm","value":"F:ATP hydrolysis activity"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0036121","properties":[{"key":"GoTerm","value":"F:double-stranded DNA helicase activity"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0051721","properties":[{"key":"GoTerm","value":"F:protein phosphatase 2A binding"},{"key":"GoEvidenceType","value":"IPI:WormBase"}],"evidences":[{"evidenceCode":"ECO:0000353","source":"PubMed","id":"12554664"}]},{"database":"GO","id":"GO:0003723","properties":[{"key":"GoTerm","value":"F:RNA binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0003724","properties":[{"key":"GoTerm","value":"F:RNA helicase activity"},{"key":"GoEvidenceType","value":"ISS:WormBase"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"PubMed","id":"10454541"}]},{"database":"GO","id":"GO:0008270","properties":[{"key":"GoTerm","value":"F:zinc ion binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0030538","properties":[{"key":"GoTerm","value":"P:embryonic genitalia morphogenesis"},{"key":"GoEvidenceType","value":"IMP:WormBase"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"2583479"}]},{"database":"GO","id":"GO:0000956","properties":[{"key":"GoTerm","value":"P:nuclear-transcribed mRNA catabolic process"},{"key":"GoEvidenceType","value":"IMP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"23828042"}]},{"database":"GO","id":"GO:0000184","properties":[{"key":"GoTerm","value":"P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"},{"key":"GoEvidenceType","value":"IMP:WormBase"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"10454541"}]},{"database":"GO","id":"GO:0035194","properties":[{"key":"GoTerm","value":"P:regulatory ncRNA-mediated post-transcriptional gene silencing"},{"key":"GoEvidenceType","value":"IMP:WormBase"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"10988072"}]},{"database":"GO","id":"GO:0030422","properties":[{"key":"GoTerm","value":"P:siRNA processing"},{"key":"GoEvidenceType","value":"IMP:WormBase"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"19800275"}]},{"database":"CDD","id":"cd21407","properties":[{"key":"EntryName","value":"1B_UPF1-like"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd18039","properties":[{"key":"EntryName","value":"DEXXQc_UPF1"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd18808","properties":[{"key":"EntryName","value":"SF1_C_Upf1"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd21400","properties":[{"key":"EntryName","value":"ZBD_UPF1-like"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"2.40.30.230:FF:000007","properties":[{"key":"EntryName","value":"Regulator of nonsense transcripts 1"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.40.50.300:FF:000097","properties":[{"key":"EntryName","value":"Regulator of nonsense transcripts 1"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.40.30.230","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"6.10.140.1240","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.300","properties":[{"key":"EntryName","value":"P-loop containing nucleotide triphosphate hydrolases"},{"key":"MatchStatus","value":"2"}]},{"database":"InterPro","id":"IPR045055","properties":[{"key":"EntryName","value":"DNA2/NAM7-like"}]},{"database":"InterPro","id":"IPR041679","properties":[{"key":"EntryName","value":"DNA2/NAM7-like_C"}]},{"database":"InterPro","id":"IPR041677","properties":[{"key":"EntryName","value":"DNA2/NAM7_AAA_11"}]},{"database":"InterPro","id":"IPR027417","properties":[{"key":"EntryName","value":"P-loop_NTPase"}]},{"database":"InterPro","id":"IPR047187","properties":[{"key":"EntryName","value":"SF1_C_Upf1"}]},{"database":"InterPro","id":"IPR040812","properties":[{"key":"EntryName","value":"UPF1_1B_dom"}]},{"database":"InterPro","id":"IPR018999","properties":[{"key":"EntryName","value":"UPF1_CH/ZBD"}]},{"database":"PANTHER","id":"PTHR10887","properties":[{"key":"EntryName","value":"DNA2/NAM7 HELICASE FAMILY"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR10887:SF364","properties":[{"key":"EntryName","value":"REGULATOR OF NONSENSE TRANSCRIPTS 1"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF13086","properties":[{"key":"EntryName","value":"AAA_11"},{"key":"MatchStatus","value":"2"}]},{"database":"Pfam","id":"PF13087","properties":[{"key":"EntryName","value":"AAA_12"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF18141","properties":[{"key":"EntryName","value":"UPF1_1B_dom"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF09416","properties":[{"key":"EntryName","value":"UPF1_Zn_bind"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF52540","properties":[{"key":"EntryName","value":"P-loop containing nucleoside triphosphate hydrolases"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51997","properties":[{"key":"EntryName","value":"UPF1_CH_RICH"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS52088","properties":[{"key":"EntryName","value":"UPF1_HELICASE"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MDDSDDEYSRSHGETLTFVDPEDDGVSIGNTQDSQFAYEQFSVPTQSSQATDLLPGGTDGTTNDLPFHDVEDDESDSEKSLTEEQHEQKLPEHACRYCGISDPLCVAKCTVCRKWFCNSNDGTSGGHIVHHMVRSQHKEAYTHKDSPCGDTQLECYRCGSKNVFNLGFIPGKKDQVVVIICRTPCASIAFQNDDNWSPEDWKSVIAEKQLLSWIVNVPSEEQVARARKITATQAVRMEELWRDHPEATVDDLNKPGLDREPDHVQLRYVDAHHYSKVFRPLVAIEAEYDRRVKESASQAVGTVRWEQGLRQSVLAFFHLPQFADGVMKLAKGDELRLKHSQTVDGSEWTKIGSVFKIPDNHGDEVGIEIRGAVDKSVMESRIMFTVDVVWNATTFERQYKALAALLNDSKAISPYLYQKLLGHPAEEMMLKFDLPRRLSVAGLPELNSSQMQAVKQVLTRPLSLIQGPPGTGKTVVSATIVYHLVQKTEGNVLVCSPSNIAVDHLAEKIHKTGLKVVRLCARSREHSETTVPYLTLQHQLKVMGGAELQKLIQLKDEAGELEFKDDLRYMQLKRVKEHELLAAADVICCTCSSAADARLSKIRTRTVLIDESTQATEPEILVSIMRGVRQLVLVGDHCQLGPVVICKKAAIAGLSQSLFERLVLLGIRPFRLQVQYRMHPVLSEFPSNVFYDGSLQNGVTENDRHMTGVDWHWPKPNKPAFFWHCSGSEELSASGTSFLNRTEAANVEKLVSKLIKAGVQPHQIGVITSYEGQRSFIVNYMHTQGTLNSKLYENVEIASVDAFQGREKDYIIVTCVRSNDILGIGFLSDPRRLNVAITRAKYGLVLVGNAKVLARHDLWHELINHYKSKEMLYEGPINALKPLNLALPKATIRTKNNIAGNANRFGIKRMQYTFNEYKSNDPSQPRLPPTYSNSQNLLSMSKLAQTFNKNVPIPAHMMDPNVYAAARNQKDRRRGDQRRPPPQAEAAMDLSQGMMSQQSQQYPPQGASSQSQYLLDGASSLSGWSQSQTTTTTTRHHHHRQNRNSQQQMSQDMDDIQQKMDDLLFSQDC","length":1069,"molWeight":120020,"crc64":"5B4C91FB49B5C24B","md5":"B9C3481B0AE9B45CBEA541492CF5366A"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":3,"COFACTOR":2,"SUBCELLULAR LOCATION":1,"PTM":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Domain":3,"Region":5,"Compositional bias":4,"Binding site":23,"Mutagenesis":2,"Sequence conflict":2},"uniParcId":"UPI000004EEDC"}}