{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"O74728","uniProtkbId":"VP384_SCHPO","entryAudit":{"firstPublicDate":"2004-07-19","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2012-10-03","entryVersion":108,"sequenceVersion":2},"annotationScore":3.0,"organism":{"scientificName":"Schizosaccharomyces pombe (strain 972 / ATCC 24843)","commonName":"Fission yeast","taxonId":284812,"lineage":["Eukaryota","Fungi","Dikarya","Ascomycota","Taphrinomycotina","Schizosaccharomycetes","Schizosaccharomycetales","Schizosaccharomycetaceae","Schizosaccharomyces"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000312","source":"PomBase","id":"SPBC1709.03"}],"value":"Vacuolar sorting protein vps3844"}},"flag":"Precursor"},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000312","source":"PomBase","id":"SPBC1709.03"}],"value":"vps3844"},"orfNames":[{"value":"SPBC1709.03"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"39477503"}],"value":"Involved in endosome-to-vacuole vesicle-mediated transport"}],"commentType":"FUNCTION"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16823372"}],"value":"Endoplasmic reticulum membrane","id":"SL-0097"},"topology":{"evidences":[{"evidenceCode":"ECO:0000255"}],"value":"Single-pass membrane protein","id":"SL-9904"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"39477503"}],"value":"Endosome membrane","id":"SL-0100"},"topology":{"evidences":[{"evidenceCode":"ECO:0000255"}],"value":"Single-pass membrane protein","id":"SL-9904"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"39477503"}],"value":"Abolishes localization of cpy1 to the vacuole with protein mistargeted to the cell surface and abolishes delivery of dye FM4-64 to the vacuole (PubMed:39477503). Sensitive to cold (PubMed:39477503)"}],"commentType":"DISRUPTION PHENOTYPE"}],"features":[{"type":"Signal","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":19,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Chain","location":{"start":{"value":20,"modifier":"EXACT"},"end":{"value":428,"modifier":"EXACT"}},"description":"Vacuolar sorting protein vps3844","featureId":"PRO_0000116770"},{"type":"Topological domain","location":{"start":{"value":20,"modifier":"EXACT"},"end":{"value":385,"modifier":"EXACT"}},"description":"Lumenal","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":386,"modifier":"EXACT"},"end":{"value":406,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":407,"modifier":"EXACT"},"end":{"value":428,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]}],"keywords":[{"id":"KW-0256","category":"Cellular component","name":"Endoplasmic reticulum"},{"id":"KW-0967","category":"Cellular component","name":"Endosome"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0732","category":"Domain","name":"Signal"},{"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"id":"KW-1133","category":"Domain","name":"Transmembrane helix"},{"id":"KW-0813","category":"Biological process","name":"Transport"}],"references":[{"referenceNumber":1,"citation":{"id":"11859360","citationType":"journal article","authors":["Wood V.","Gwilliam R.","Rajandream M.A.","Lyne M.H.","Lyne R.","Stewart A.","Sgouros J.G.","Peat N.","Hayles J.","Baker S.G.","Basham D.","Bowman S.","Brooks K.","Brown D.","Brown S.","Chillingworth T.","Churcher C.M.","Collins M.","Connor R.","Cronin A.","Davis P.","Feltwell T.","Fraser A.","Gentles S.","Goble A.","Hamlin N.","Harris D.E.","Hidalgo J.","Hodgson G.","Holroyd S.","Hornsby T.","Howarth S.","Huckle E.J.","Hunt S.","Jagels K.","James K.D.","Jones L.","Jones M.","Leather S.","McDonald S.","McLean J.","Mooney P.","Moule S.","Mungall K.L.","Murphy L.D.","Niblett D.","Odell C.","Oliver K.","O'Neil S.","Pearson D.","Quail M.A.","Rabbinowitsch E.","Rutherford K.M.","Rutter S.","Saunders D.","Seeger K.","Sharp S.","Skelton J.","Simmonds M.N.","Squares R.","Squares S.","Stevens K.","Taylor K.","Taylor R.G.","Tivey A.","Walsh S.V.","Warren T.","Whitehead S.","Woodward J.R.","Volckaert G.","Aert R.","Robben J.","Grymonprez B.","Weltjens I.","Vanstreels E.","Rieger M.","Schaefer M.","Mueller-Auer S.","Gabel C.","Fuchs M.","Duesterhoeft A.","Fritzc C.","Holzer E.","Moestl D.","Hilbert H.","Borzym K.","Langer I.","Beck A.","Lehrach H.","Reinhardt R.","Pohl T.M.","Eger P.","Zimmermann W.","Wedler H.","Wambutt R.","Purnelle B.","Goffeau A.","Cadieu E.","Dreano S.","Gloux S.","Lelaure V.","Mottier S.","Galibert F.","Aves S.J.","Xiang Z.","Hunt C.","Moore K.","Hurst S.M.","Lucas M.","Rochet M.","Gaillardin C.","Tallada V.A.","Garzon A.","Thode G.","Daga R.R.","Cruzado L.","Jimenez J.","Sanchez M.","del Rey F.","Benito J.","Dominguez A.","Revuelta J.L.","Moreno S.","Armstrong J.","Forsburg S.L.","Cerutti L.","Lowe T.","McCombie W.R.","Paulsen I.","Potashkin J.","Shpakovski G.V.","Ussery D.","Barrell B.G.","Nurse P."],"citationCrossReferences":[{"database":"PubMed","id":"11859360"},{"database":"DOI","id":"10.1038/nature724"}],"title":"The genome sequence of Schizosaccharomyces pombe.","publicationDate":"2002","journal":"Nature","firstPage":"871","lastPage":"880","volume":"415"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"972 / ATCC 24843","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"21511999","citationType":"journal article","authors":["Rhind N.","Chen Z.","Yassour M.","Thompson D.A.","Haas B.J.","Habib N.","Wapinski I.","Roy S.","Lin M.F.","Heiman D.I.","Young S.K.","Furuya K.","Guo Y.","Pidoux A.","Chen H.M.","Robbertse B.","Goldberg J.M.","Aoki K.","Bayne E.H.","Berlin A.M.","Desjardins C.A.","Dobbs E.","Dukaj L.","Fan L.","FitzGerald M.G.","French C.","Gujja S.","Hansen K.","Keifenheim D.","Levin J.Z.","Mosher R.A.","Mueller C.A.","Pfiffner J.","Priest M.","Russ C.","Smialowska A.","Swoboda P.","Sykes S.M.","Vaughn M.","Vengrova S.","Yoder R.","Zeng Q.","Allshire R.","Baulcombe D.","Birren B.W.","Brown W.","Ekwall K.","Kellis M.","Leatherwood J.","Levin H.","Margalit H.","Martienssen R.","Nieduszynski C.A.","Spatafora J.W.","Friedman N.","Dalgaard J.Z.","Baumann P.","Niki H.","Regev A.","Nusbaum C."],"citationCrossReferences":[{"database":"PubMed","id":"21511999"},{"database":"DOI","id":"10.1126/science.1203357"}],"title":"Comparative functional genomics of the fission yeasts.","publicationDate":"2011","journal":"Science","firstPage":"930","lastPage":"936","volume":"332"},"referencePositions":["REVISION OF GENE MODEL"]},{"referenceNumber":3,"citation":{"id":"16823372","citationType":"journal article","authors":["Matsuyama A.","Arai R.","Yashiroda Y.","Shirai A.","Kamata A.","Sekido S.","Kobayashi Y.","Hashimoto A.","Hamamoto M.","Hiraoka Y.","Horinouchi S.","Yoshida M."],"citationCrossReferences":[{"database":"PubMed","id":"16823372"},{"database":"DOI","id":"10.1038/nbt1222"}],"title":"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.","publicationDate":"2006","journal":"Nat. Biotechnol.","firstPage":"841","lastPage":"847","volume":"24"},"referencePositions":["SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS]"]},{"referenceNumber":4,"citation":{"id":"39477503","citationType":"journal article","authors":["Inagawa T.","Ohkubo K.","Watanabe M.","Morita T.","Higuchi Y.","Maekawa H.","Takegawa K."],"citationCrossReferences":[{"database":"PubMed","id":"39477503"},{"database":"DOI","id":"10.2323/jgam.2024.10.001"}],"title":"A DUF3844 domain-containing protein is required for vacuolar protein sorting in Schizosaccharomyces pombe.","publicationDate":"2025","journal":"J. Gen. Appl. Microbiol.","firstPage":"0","lastPage":"0","volume":"70"},"referencePositions":["FUNCTION","SUBCELLULAR LOCATION","DISRUPTION PHENOTYPE"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"CU329671","properties":[{"key":"ProteinId","value":"CAA21242.2"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"T39631","properties":[{"key":"EntryName","value":"T39631"}]},{"database":"RefSeq","id":"NP_595436.2","properties":[{"key":"NucleotideSequenceId","value":"NM_001021344.3"}]},{"database":"AlphaFoldDB","id":"O74728","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"O74728","properties":[{"key":"Description","value":"-"}]},{"database":"iPTMnet","id":"O74728","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"284812-O74728","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"spo:2540196","properties":[{"key":"Description","value":"-"}]},{"database":"PomBase","id":"SPBC1709.03","properties":[{"key":"GeneName","value":"vps3844"}]},{"database":"VEuPathDB","id":"FungiDB:SPBC1709.03","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"ENOG502S64Q","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"HOGENOM","id":"CLU_571250_0_0_1","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"O74728","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"KTTKWAG","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"PRO","id":"PR:O74728","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000002485","properties":[{"key":"Component","value":"Chromosome II"}]},{"database":"GO","id":"GO:0005783","properties":[{"key":"GoTerm","value":"C:endoplasmic reticulum"},{"key":"GoEvidenceType","value":"HDA:PomBase"}],"evidences":[{"evidenceCode":"ECO:0007005","source":"PubMed","id":"16823372"}]},{"database":"GO","id":"GO:0005789","properties":[{"key":"GoTerm","value":"C:endoplasmic reticulum membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0010008","properties":[{"key":"GoTerm","value":"C:endosome membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"InterPro","id":"IPR053065","properties":[{"key":"EntryName","value":"Archenteron_Induction-Rel"}]},{"database":"InterPro","id":"IPR024382","properties":[{"key":"EntryName","value":"Vps3844_C"}]},{"database":"InterPro","id":"IPR049205","properties":[{"key":"EntryName","value":"Vps3844_N"}]},{"database":"PANTHER","id":"PTHR36853:SF1","properties":[{"key":"EntryName","value":"DUF3844 DOMAIN-CONTAINING PROTEIN"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR36853","properties":[{"key":"EntryName","value":"EXPRESSED PROTEIN"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF21656","properties":[{"key":"EntryName","value":"DUF6859"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF12955","properties":[{"key":"EntryName","value":"Vps3844_C"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MSLLRYFIFFIGGCTLALASGVSYTEASVYMSGLSGNENEVPSISPSDSRLVFAHKLGVSRFHKLKSHSGARKIINEMLHSHSELSSFFYAEKSNTFLTIAGLAESELFDTLNPAFRISSCPSSNAFASLMCRYERQLDSLDNGKTTDIYLNDIGGVISIHDSTGVYSQITTTPYKSLEDYEQLFGEDKASMFDISVKQDRVFLSEIYALKILVEFLSSKSQQERSDTSIVIGQLVGLEALYEKYGKDSKIYQVARENMMTLLSMIKQVSSTYTFILLPPIDSSETSTRYHKRQVDMEAFGEEIEEVVASYEKNYNGRCYISEEACSKATNDCSGHGRCSKYGQLDSCYVCQCSNSVVSNAAGQNKTIRWAGESCSKQDISVEFQFFFWFTIIGFGLLIFSIMLLFSIGQEELANVLTSTATVIKKTT","length":428,"molWeight":47740,"crc64":"8F94BE6A03C0E54E","md5":"18A50881C04D617D41F293A3DFD71404"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBCELLULAR LOCATION":1,"DISRUPTION PHENOTYPE":1},"countByFeatureType":{"Signal":1,"Chain":1,"Topological domain":2,"Transmembrane":1},"uniParcId":"UPI000228F3B4"}}