{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"O74504","secondaryAccessions":["O74961"],"uniProtkbId":"DUC1_SCHPO","entryAudit":{"firstPublicDate":"2004-03-29","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2004-03-29","entryVersion":110,"sequenceVersion":2},"annotationScore":5.0,"organism":{"scientificName":"Schizosaccharomyces pombe (strain 972 / ATCC 24843)","commonName":"Fission yeast","taxonId":284812,"lineage":["Eukaryota","Fungi","Dikarya","Ascomycota","Taphrinomycotina","Schizosaccharomycetes","Schizosaccharomycetales","Schizosaccharomycetaceae","Schizosaccharomyces"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000312","source":"PomBase","id":"SPCC594.01"}],"value":"DUF1769 family protein duc1"}},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"39239853"}],"value":"Domain of unknown function at the cortex"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"39239853"}],"value":"duc1"},"orfNames":[{"value":"SPCC594.01"},{"value":"SPCC736.16"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"39239853"}],"value":"Promotes the proper distribution of phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2/PIP2) synthesis at the cell membrane (PubMed:39239853). May bind phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2/PIP2) and is required for robust anchoring of the contractile ring to the cell membrane (PubMed:39239853)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"39239853"}],"value":"Interacts (via FFAT-motif) with scs2 (via MSP domain); the interaction is direct and serves to restrict the localization of duc1 to areas of cell membrane-endoplasmic reticulum contact sites, and away from the cell division site"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"39239853"}],"value":"Localizes to cell membrane-endoplasmic reticulum contact sites in a manner dependent on the VAMP-associated proteins (VAPs) scs2 and scs22 (PubMed:39239853). Less abundant at the cell tips during interphase; during cytokinesis it relocalizes towards the cell ends and is excluded from the medial region (PubMed:39239853)"}]},"subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"39239853"}],"value":"Cell membrane","id":"SL-0039"},"topology":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"39239853"}],"value":"Peripheral membrane protein","id":"SL-9903"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"39239853"}],"value":"Reduces the amount of phosphatidylinositol 4-phosphate 5-kinase its3 at the cell membrane (PubMed:39239853). Leads to sliding of the contractile ring away from the midpoint of the cell, and consequently off-center septation (PubMed:39239853)"}],"commentType":"DISRUPTION PHENOTYPE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the UPF0590 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":791,"modifier":"EXACT"}},"description":"DUF1769 family protein duc1","featureId":"PRO_0000116812"},{"type":"Region","location":{"start":{"value":158,"modifier":"EXACT"},"end":{"value":189,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":381,"modifier":"EXACT"},"end":{"value":505,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":542,"modifier":"EXACT"},"end":{"value":606,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":630,"modifier":"EXACT"},"end":{"value":658,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Motif","location":{"start":{"value":373,"modifier":"EXACT"},"end":{"value":379,"modifier":"EXACT"}},"description":"FFAT","evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"39239853"}]},{"type":"Compositional bias","location":{"start":{"value":174,"modifier":"EXACT"},"end":{"value":184,"modifier":"EXACT"}},"description":"Low complexity","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":415,"modifier":"EXACT"},"end":{"value":426,"modifier":"EXACT"}},"description":"Basic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":444,"modifier":"EXACT"},"end":{"value":453,"modifier":"EXACT"}},"description":"Low complexity","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":455,"modifier":"EXACT"},"end":{"value":473,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":477,"modifier":"EXACT"},"end":{"value":487,"modifier":"EXACT"}},"description":"Basic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":555,"modifier":"EXACT"},"end":{"value":564,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":574,"modifier":"EXACT"},"end":{"value":586,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":587,"modifier":"EXACT"},"end":{"value":601,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Modified residue","location":{"start":{"value":374,"modifier":"EXACT"},"end":{"value":374,"modifier":"EXACT"}},"description":"Phosphotyrosine","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18257517"}]},{"type":"Modified residue","location":{"start":{"value":376,"modifier":"EXACT"},"end":{"value":376,"modifier":"EXACT"}},"description":"Phosphothreonine","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18257517"}]},{"type":"Modified residue","location":{"start":{"value":477,"modifier":"EXACT"},"end":{"value":477,"modifier":"EXACT"}},"description":"Phosphoserine","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18257517"}]},{"type":"Modified residue","location":{"start":{"value":493,"modifier":"EXACT"},"end":{"value":493,"modifier":"EXACT"}},"description":"Phosphoserine","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18257517"}]},{"type":"Modified residue","location":{"start":{"value":574,"modifier":"EXACT"},"end":{"value":574,"modifier":"EXACT"}},"description":"Phosphoserine","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18257517"}]},{"type":"Mutagenesis","location":{"start":{"value":374,"modifier":"EXACT"},"end":{"value":375,"modifier":"EXACT"}},"description":"Leads to mislocalization of duc1 to the area of cell division.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"39239853"}],"alternativeSequence":{"originalSequence":"YF","alternativeSequences":["AA"]}}],"keywords":[{"id":"KW-1003","category":"Cellular component","name":"Cell membrane"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-0597","category":"PTM","name":"Phosphoprotein"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"}],"references":[{"referenceNumber":1,"citation":{"id":"11859360","citationType":"journal article","authors":["Wood V.","Gwilliam R.","Rajandream M.A.","Lyne M.H.","Lyne R.","Stewart A.","Sgouros J.G.","Peat N.","Hayles J.","Baker S.G.","Basham D.","Bowman S.","Brooks K.","Brown D.","Brown S.","Chillingworth T.","Churcher C.M.","Collins M.","Connor R.","Cronin A.","Davis P.","Feltwell T.","Fraser A.","Gentles S.","Goble A.","Hamlin N.","Harris D.E.","Hidalgo J.","Hodgson G.","Holroyd S.","Hornsby T.","Howarth S.","Huckle E.J.","Hunt S.","Jagels K.","James K.D.","Jones L.","Jones M.","Leather S.","McDonald S.","McLean J.","Mooney P.","Moule S.","Mungall K.L.","Murphy L.D.","Niblett D.","Odell C.","Oliver K.","O'Neil S.","Pearson D.","Quail M.A.","Rabbinowitsch E.","Rutherford K.M.","Rutter S.","Saunders D.","Seeger K.","Sharp S.","Skelton J.","Simmonds M.N.","Squares R.","Squares S.","Stevens K.","Taylor K.","Taylor R.G.","Tivey A.","Walsh S.V.","Warren T.","Whitehead S.","Woodward J.R.","Volckaert G.","Aert R.","Robben J.","Grymonprez B.","Weltjens I.","Vanstreels E.","Rieger M.","Schaefer M.","Mueller-Auer S.","Gabel C.","Fuchs M.","Duesterhoeft A.","Fritzc C.","Holzer E.","Moestl D.","Hilbert H.","Borzym K.","Langer I.","Beck A.","Lehrach H.","Reinhardt R.","Pohl T.M.","Eger P.","Zimmermann W.","Wedler H.","Wambutt R.","Purnelle B.","Goffeau A.","Cadieu E.","Dreano S.","Gloux S.","Lelaure V.","Mottier S.","Galibert F.","Aves S.J.","Xiang Z.","Hunt C.","Moore K.","Hurst S.M.","Lucas M.","Rochet M.","Gaillardin C.","Tallada V.A.","Garzon A.","Thode G.","Daga R.R.","Cruzado L.","Jimenez J.","Sanchez M.","del Rey F.","Benito J.","Dominguez A.","Revuelta J.L.","Moreno S.","Armstrong J.","Forsburg S.L.","Cerutti L.","Lowe T.","McCombie W.R.","Paulsen I.","Potashkin J.","Shpakovski G.V.","Ussery D.","Barrell B.G.","Nurse P."],"citationCrossReferences":[{"database":"PubMed","id":"11859360"},{"database":"DOI","id":"10.1038/nature724"}],"title":"The genome sequence of Schizosaccharomyces pombe.","publicationDate":"2002","journal":"Nature","firstPage":"871","lastPage":"880","volume":"415"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"972 / ATCC 24843","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"18257517","citationType":"journal article","authors":["Wilson-Grady J.T.","Villen J.","Gygi S.P."],"citationCrossReferences":[{"database":"PubMed","id":"18257517"},{"database":"DOI","id":"10.1021/pr7006335"}],"title":"Phosphoproteome analysis of fission yeast.","publicationDate":"2008","journal":"J. Proteome Res.","firstPage":"1088","lastPage":"1097","volume":"7"},"referencePositions":["PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-374; THR-376; SER-477; SER-493 AND SER-574","IDENTIFICATION BY MASS SPECTROMETRY"]},{"referenceNumber":3,"citation":{"id":"39239853","citationType":"journal article","authors":["Willet A.H.","Park J.S.","Snider C.E.","Huang J.J.","Chen J.S.","Gould K.L."],"citationCrossReferences":[{"database":"PubMed","id":"39239853"},{"database":"DOI","id":"10.1242/jcs.262347"}],"title":"Fission yeast Duc1 links to ER-PM contact sites and influences PM lipid composition and cytokinetic ring anchoring.","publicationDate":"2024","journal":"J. Cell Sci.","firstPage":"262347","lastPage":"262347","volume":"137"},"referencePositions":["FUNCTION","INTERACTION WITH SCS2","SUBCELLULAR LOCATION","DISRUPTION PHENOTYPE","MOTIF FFAT","MUTAGENESIS OF 374-TYR-PHE-375"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"CU329672","properties":[{"key":"ProteinId","value":"CAA19280.2"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"T41573","properties":[{"key":"EntryName","value":"T41573"}]},{"database":"AlphaFoldDB","id":"O74504","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"275292","properties":[{"key":"Interactions","value":"45"}]},{"database":"FunCoup","id":"O74504","properties":[{"key":"Number of interactors","value":"1"}]},{"database":"STRING","id":"284812.O74504","properties":[{"key":"Description","value":"-"}]},{"database":"iPTMnet","id":"O74504","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"284812-O74504","properties":[{"key":"Description","value":"-"}]},{"database":"PomBase","id":"SPCC594.01","properties":[{"key":"GeneName","value":"duc1"}]},{"database":"VEuPathDB","id":"FungiDB:SPCC594.01","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"ENOG502RXNE","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"HOGENOM","id":"CLU_021760_0_0_1","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"O74504","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"APFNAGM","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"PRO","id":"PR:O74504","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000002485","properties":[{"key":"Component","value":"Chromosome III"}]},{"database":"GO","id":"GO:0032541","properties":[{"key":"GoTerm","value":"C:cortical endoplasmic reticulum"},{"key":"GoEvidenceType","value":"IDA:PomBase"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"39239853"}]},{"database":"GO","id":"GO:0140268","properties":[{"key":"GoTerm","value":"C:endoplasmic reticulum-plasma membrane contact site"},{"key":"GoEvidenceType","value":"IPI:PomBase"}],"evidences":[{"evidenceCode":"ECO:0000353","source":"PubMed","id":"39239853"}]},{"database":"GO","id":"GO:0016328","properties":[{"key":"GoTerm","value":"C:lateral plasma membrane"},{"key":"GoEvidenceType","value":"IDA:PomBase"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"39239853"}]},{"database":"GO","id":"GO:0005886","properties":[{"key":"GoTerm","value":"C:plasma membrane"},{"key":"GoEvidenceType","value":"EXP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"39239853"}]},{"database":"GO","id":"GO:0005546","properties":[{"key":"GoTerm","value":"F:phosphatidylinositol-4,5-bisphosphate binding"},{"key":"GoEvidenceType","value":"IMP:PomBase"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"39239853"}]},{"database":"GO","id":"GO:0140550","properties":[{"key":"GoTerm","value":"F:phosphatidylinositol-4,5-bisphosphate sensor activity"},{"key":"GoEvidenceType","value":"IDA:PomBase"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"39239853"}]},{"database":"GO","id":"GO:0007009","properties":[{"key":"GoTerm","value":"P:plasma membrane organization"},{"key":"GoEvidenceType","value":"IMP:PomBase"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"39239853"}]},{"database":"GO","id":"GO:1902648","properties":[{"key":"GoTerm","value":"P:positive regulation of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate biosynthetic process"},{"key":"GoEvidenceType","value":"IMP:PomBase"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"39239853"}]},{"database":"InterPro","id":"IPR013897","properties":[{"key":"EntryName","value":"Duc1"}]},{"database":"InterPro","id":"IPR055395","properties":[{"key":"EntryName","value":"Duc1_FFAT-like"}]},{"database":"PANTHER","id":"PTHR34826","properties":[{"key":"EntryName","value":"UPF0590 PROTEIN C409.17C"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR34826:SF1","properties":[{"key":"EntryName","value":"UPF0590 PROTEIN C594.01"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF08588","properties":[{"key":"EntryName","value":"Duc1"},{"key":"MatchStatus","value":"2"}]},{"database":"Pfam","id":"PF24794","properties":[{"key":"EntryName","value":"Duc1_FFAT_like"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MKLKVSIGCDAQHTQIAWVNYEGRPTEVDGPLWVGRILVRVRDFDGFTPDGSPPKRDSEYFRGRSRKFQIQAEGRFKKEYNGDQVIYGTQFDHMISTFPESAFRAGMRIAKYIDPAVYYDKYARSPYIMSPFVACVNTLSAWPAPSRLEDAVISLVEADSQESDTESLPEINDSSDVSLSDLPSTNVTPKKTTTQIDVQTNIVTPPITVTAVPDSPNPPAATPATVADNDDLSIVSSDSGNTTAPRKNRHRYWSFAGFSDSTRFSHLNVPAAIPDAPSVKTETSQNVSQLSVKSAATSLAPVEDDEDDELLLHRHITNKHKDFNLHVKQLGLLHKPSLDLEEGYIMDENYGKDVYKHGISNVPDDENEDDLQRYFTALEMQQDQKEQHKKSKNKDPFRKITHPSLHLGKFSTPKLIKRMSLRSKKSLRNDSKSDDVGNSTHRFSTASAASTSAVKTEKEKKMSAPRRSLDKLIRIGSLHRHHHHHHKTDLIESDSGIEASESNRRKSDIFSFSGRNSFSVSRPSSSHSTLSYANDSASSAVNVAGETGSLPPLREQTSITSGVPPSNRLKKHVSTPEKIVEERSIDEVSQSNTPSSKQLPQSVDGKTVTANANSTTVAKQPTSNVALTRPKPVRTATSQSKIPKPVKHIPSDSNNLDPQLGPWRFANPKVDPIEDNSFIFGEHKSVKERRKYFSSSKFCRENFFYDKDVVYCMSFFSPHMDFNTFNLNIGPIRLNVYKHLNSDGHQPIRYMMRETDDEDAVMFVVEFDLLEDDDETVLAEQAKERERRKQT","length":791,"molWeight":88749,"crc64":"FFDA38EBED428C93","md5":"CD831D31F9FA1247684354791EDEE078"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"DISRUPTION PHENOTYPE":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Region":4,"Motif":1,"Compositional bias":8,"Modified residue":5,"Mutagenesis":1},"uniParcId":"UPI00001868E9"}}