{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"O56124","secondaryAccessions":["Q9YR18"],"uniProtkbId":"ORF3_PCV2","entryAudit":{"firstPublicDate":"2005-10-25","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"1998-06-01","entryVersion":62,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Porcine circovirus 2","commonName":"PCV2","taxonId":85708,"lineage":["Viruses","Floreoviria","Shotokuvirae","Cressdnaviricota","Arfiviricetes","Cirlivirales","Circoviridae","Circovirus","Circovirus porcine2"]},"organismHosts":[{"scientificName":"Sus scrofa","commonName":"Pig","taxonId":9823}],"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Protein ORF3"}}},"genes":[{"orfNames":[{"value":"ORF3"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15956572"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25575706"}],"value":"Plays a role in modulating host cell signaling by binding to and degrading host RGS16 (PubMed:25575706). Not necessary for virus replication (PubMed:15956572)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25575706"}],"value":"Interacts with host RGS16"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"26854343"}],"value":"Expressed in cytoplasm in early stages of PCV2 infection and then accumulated in nucleus over time"}]},"subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"26854343"}],"value":"Host cytoplasm","id":"SL-0381"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"26854343"}],"value":"Host nucleus","id":"SL-0414"}}]}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":104,"modifier":"EXACT"}},"description":"Protein ORF3","featureId":"PRO_0000133087"},{"type":"Region","location":{"start":{"value":78,"modifier":"EXACT"},"end":{"value":104,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Motif","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":35,"modifier":"EXACT"}},"description":"Nuclear export signal","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"26854343"}]}],"keywords":[{"id":"KW-0053","category":"Biological process","name":"Apoptosis"},{"id":"KW-1035","category":"Cellular component","name":"Host cytoplasm"},{"id":"KW-1048","category":"Cellular component","name":"Host nucleus"},{"id":"KW-0945","category":"Biological process","name":"Host-virus interaction"}],"references":[{"referenceNumber":1,"citation":{"id":"9573301","citationType":"journal article","authors":["Hamel A.L.","Lin L.L.","Nayar G.P."],"citationCrossReferences":[{"database":"PubMed","id":"9573301"},{"database":"DOI","id":"10.1128/jvi.72.6.5262-5267.1998"}],"title":"Nucleotide sequence of porcine circovirus associated with postweaning multisystemic wasting syndrome in pigs.","publicationDate":"1998","journal":"J. Virol.","firstPage":"5262","lastPage":"5267","volume":"72"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"]},{"referenceNumber":2,"citation":{"id":"9747726","citationType":"journal article","authors":["Meehan B.M.","McNeilly F.","Todd D.","Kennedy S.","Jewhurst V.A.","Ellis J.A.","Hassard L.E.","Clark E.G.","Haines D.M.","Allan G.M."],"citationCrossReferences":[{"database":"PubMed","id":"9747726"},{"database":"DOI","id":"10.1099/0022-1317-79-9-2171"}],"title":"Characterization of novel circovirus DNAs associated with wasting syndromes in pigs.","publicationDate":"1998","journal":"J. Gen. Virol.","firstPage":"2171","lastPage":"2179","volume":"79"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"]},{"referenceNumber":3,"citation":{"id":"15956572","citationType":"journal article","authors":["Liu J.","Chen I.","Kwang J."],"citationCrossReferences":[{"database":"PubMed","id":"15956572"},{"database":"DOI","id":"10.1128/jvi.79.13.8262-8274.2005"}],"title":"Characterization of a previously unidentified viral protein in porcine circovirus type 2-infected cells and its role in virus-induced apoptosis.","publicationDate":"2005","journal":"J. Virol.","firstPage":"8262","lastPage":"8274","volume":"79"},"referencePositions":["FUNCTION"]},{"referenceNumber":4,"citation":{"id":"25011695","citationType":"journal article","authors":["Lv Q.Z.","Guo K.K.","Zhang Y.M."],"citationCrossReferences":[{"database":"PubMed","id":"25011695"},{"database":"DOI","id":"10.1007/s11262-014-1099-z"}],"title":"Current understanding of genomic DNA of porcine circovirus type 2.","publicationDate":"2014","journal":"Virus Genes","firstPage":"1","lastPage":"10","volume":"49"},"referencePositions":["REVIEW"]},{"referenceNumber":5,"citation":{"id":"25575706","citationType":"journal article","authors":["Choi C.Y.","Rho S.B.","Kim H.S.","Han J.","Bae J.","Lee S.J.","Jung W.W.","Chun T."],"citationCrossReferences":[{"database":"PubMed","id":"25575706"},{"database":"DOI","id":"10.1099/vir.0.000046"}],"title":"The ORF3 protein of porcine circovirus type 2 promotes secretion of IL-6 and IL-8 in porcine epithelial cells by facilitating proteasomal degradation of regulator of G protein signalling 16 through physical interaction.","publicationDate":"2015","journal":"J. Gen. Virol.","firstPage":"1098","lastPage":"1108","volume":"96"},"referencePositions":["FUNCTION","INTERACTION WITH HOST RGS16"]},{"referenceNumber":6,"citation":{"id":"26854343","citationType":"journal article","authors":["Gu J.","Wang L.","Jin Y.","Lin C.","Wang H.","Zhou N.","Xing G.","Liao M.","Zhou J."],"citationCrossReferences":[{"database":"PubMed","id":"26854343"},{"database":"DOI","id":"10.1016/j.vetmic.2016.01.006"}],"title":"Characterization of specific antigenic epitopes and the nuclear export signal of the Porcine circovirus 2 ORF3 protein.","publicationDate":"2016","journal":"Vet. Microbiol.","firstPage":"40","lastPage":"50","volume":"184"},"referencePositions":["SUBCELLULAR LOCATION"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AF027217","properties":[{"key":"ProteinId","value":"AAC59464.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AF055391","properties":[{"key":"ProteinId","value":"AAC35300.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AF055392","properties":[{"key":"ProteinId","value":"AAC35311.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"GO","id":"GO:0030430","properties":[{"key":"GoTerm","value":"C:host cell cytoplasm"},{"key":"GoEvidenceType","value":"EXP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"26854343"}]},{"database":"GO","id":"GO:0042025","properties":[{"key":"GoTerm","value":"C:host cell nucleus"},{"key":"GoEvidenceType","value":"EXP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"26854343"}]},{"database":"InterPro","id":"IPR008500","properties":[{"key":"EntryName","value":"Circovirus_Orf3"}]},{"database":"Pfam","id":"PF05614","properties":[{"key":"EntryName","value":"Circovir_ORF3"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MVTIPPLVSRWFPVCGFRVCKISSPFAFTTPRWPHNDVYIGLPITLLHFPAHFQKFSQPAEISDKRYRVLLCNGHQTPALQQGTHSSRQVTPLSLRSRSSTFNK","length":104,"molWeight":11879,"crc64":"DD83D288A824E869","md5":"93D65F8FC9F7D7354BF3EE7097E66965"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1},"countByFeatureType":{"Chain":1,"Region":1,"Motif":1},"uniParcId":"UPI00000FA082"}}