{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"O53332","secondaryAccessions":["F2GJP1","I6YFG2","L0TC23"],"uniProtkbId":"HIGB3_MYCTU","entryAudit":{"firstPublicDate":"2015-04-29","lastAnnotationUpdateDate":"2026-06-10","lastSequenceUpdateDate":"1998-06-01","entryVersion":116,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)","taxonId":83332,"lineage":["Bacteria","Bacillati","Actinomycetota","Actinomycetes","Mycobacteriales","Mycobacteriaceae","Mycobacterium","Mycobacterium tuberculosis complex"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"24662523"}],"value":"Putative toxin HigB3"}}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"24662523"}],"value":"higB3"},"orderedLocusNames":[{"value":"Rv3182"},{"value":"RVBD_3182"}],"orfNames":[{"value":"P425_03314"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20011113"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"24662523"}],"value":"Putative toxic component of a type II toxin-antitoxin (TA) system. Its cognate antitoxin would be HigA3. Not toxic upon expression in M.smegmatis"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the mycobacterial HigB family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":114,"modifier":"EXACT"}},"description":"Putative toxin HigB3","featureId":"PRO_0000432914"}],"keywords":[{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-1277","category":"Biological process","name":"Toxin-antitoxin system"}],"references":[{"referenceNumber":1,"citation":{"id":"9634230","citationType":"journal article","authors":["Cole S.T.","Brosch R.","Parkhill J.","Garnier T.","Churcher C.M.","Harris D.E.","Gordon S.V.","Eiglmeier K.","Gas S.","Barry C.E. III","Tekaia F.","Badcock K.","Basham D.","Brown D.","Chillingworth T.","Connor R.","Davies R.M.","Devlin K.","Feltwell T.","Gentles S.","Hamlin N.","Holroyd S.","Hornsby T.","Jagels K.","Krogh A.","McLean J.","Moule S.","Murphy L.D.","Oliver S.","Osborne J.","Quail M.A.","Rajandream M.A.","Rogers J.","Rutter S.","Seeger K.","Skelton S.","Squares S.","Squares R.","Sulston J.E.","Taylor K.","Whitehead S.","Barrell B.G."],"citationCrossReferences":[{"database":"PubMed","id":"9634230"},{"database":"DOI","id":"10.1038/31159"}],"title":"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.","publicationDate":"1998","journal":"Nature","firstPage":"537","lastPage":"544","volume":"393"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"ATCC 25618 / H37Rv","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"CI-E4V7NQPHD86T9","citationType":"submission","authoringGroup":["The Broad Institute Genome Sequencing Platform"],"authors":["Galagan J.","Kreiswirth B.","Dobos K.","Fortune S.","Fitzgerald M.","Young S.K.","Zeng Q.","Gargeya S.","Abouelleil A.","Alvarado L.","Berlin A.M.","Chapman S.B.","Gainer-Dewar J.","Goldberg J.","Gnerre S.","Griggs A.","Gujja S.","Hansen M.","Howarth C.","Imamovic A.","Larimer J.","McCowan C.","Murphy C.","Pearson M.","Poon T.","Priest M.","Roberts A.","Saif S.","Shea T.","Sykes S.","Wortman J.","Nusbaum C.","Birren B."],"title":"The genome sequence of Mycobacterium tuberculosis H37Rv.","publicationDate":"NOV-2013","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"ATCC 25618 / H37Rv","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"CI-20FJD63876B4J","citationType":"submission","authoringGroup":["The Broad Institute Genomics Platform","The Broad Institute Genome Sequencing Center for Infectious Disease"],"authors":["Earl A.M.","Kreiswirth B.","Gomez J.","Victor T.","Desjardins C.","Abeel T.","Young S.","Zeng Q.","Gargeya S.","Abouelleil A.","Alvarado L.","Chapman S.B.","Gainer-Dewar J.","Goldberg J.","Griggs A.","Gujja S.","Hansen M.","Howarth C.","Imamovic A.","Larimer J.","Murphy C.","Naylor J.","Pearson M.","Poon T.W.","Priest M.","Roberts A.","Saif S.","Shea T.","Sykes S.","Wortman J.","Nusbaum C.","Birren B."],"title":"The genome sequence of Mycobacterium tuberculosis H37Rv.","publicationDate":"APR-2014","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"ATCC 25618 / H37Rv","type":"STRAIN"}]},{"referenceNumber":4,"citation":{"id":"20011113","citationType":"journal article","authors":["Ramage H.R.","Connolly L.E.","Cox J.S."],"citationCrossReferences":[{"database":"PubMed","id":"20011113"},{"database":"DOI","id":"10.1371/journal.pgen.1000767"}],"title":"Comprehensive functional analysis of Mycobacterium tuberculosis toxin-antitoxin systems: implications for pathogenesis, stress responses, and evolution.","publicationDate":"2009","journal":"PLoS Genet.","firstPage":"E1000767","lastPage":"E1000767","volume":"5"},"referencePositions":["EXPRESSION IN M.SMEGMATIS"],"referenceComments":[{"value":"ATCC 35801 / TMC 107 / Erdman","type":"STRAIN"}]},{"referenceNumber":5,"citation":{"id":"24662523","citationType":"journal article","authors":["Sala A.","Bordes P.","Genevaux P."],"citationCrossReferences":[{"database":"PubMed","id":"24662523"},{"database":"DOI","id":"10.3390/toxins6031002"}],"title":"Multiple toxin-antitoxin systems in Mycobacterium tuberculosis.","publicationDate":"2014","journal":"Toxins","firstPage":"1002","lastPage":"1020","volume":"6"},"referencePositions":["DISCUSSION OF POSSIBLE FUNCTION"],"referenceComments":[{"value":"ATCC 25618 / H37Rv","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AL123456","properties":[{"key":"ProteinId","value":"CCP45993.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"CP003248","properties":[{"key":"ProteinId","value":"AFN51175.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"JLDD01000038","properties":[{"key":"ProteinId","value":"KBJ29228.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"NP_217698.1","properties":[{"key":"NucleotideSequenceId","value":"NC_000962.3"}]},{"database":"RefSeq","id":"WP_003899954.1","properties":[{"key":"NucleotideSequenceId","value":"NC_000962.3"}]},{"database":"AlphaFoldDB","id":"O53332","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"O53332","properties":[{"key":"Description","value":"-"}]},{"database":"STRING","id":"83332.Rv3182","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"83332-Rv3182","properties":[{"key":"Description","value":"-"}]},{"database":"DNASU","id":"888795","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"888795","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"mtu:Rv3182","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"mtv:RVBD_3182","properties":[{"key":"Description","value":"-"}]},{"database":"PATRIC","id":"fig|83332.111.peg.3545","properties":[{"key":"GeneDesignation","value":"-"}]},{"database":"TubercuList","id":"Rv3182","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"COG4683","properties":[{"key":"ToxonomicScope","value":"Bacteria"}]},{"database":"HOGENOM","id":"CLU_107454_1_1_11","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"O53332","properties":[{"key":"Description","value":"-"}]},{"database":"OrthoDB","id":"330810at2","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"O53332","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000001584","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"AbasyAtlas","id":"O53332","properties":[{"key":"Description","value":"-"}]},{"database":"InterPro","id":"IPR009241","properties":[{"key":"EntryName","value":"HigB-like"}]},{"database":"Pfam","id":"PF05973","properties":[{"key":"EntryName","value":"Gp49"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLLGGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG","length":114,"molWeight":12692,"crc64":"119D0463FA24AB59","md5":"56E1F1852248EFC81864D4D85526D578"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1},"uniParcId":"UPI00000CCB68"}}