{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"O51076","uniProtkbId":"BPUR_BORBU","entryAudit":{"firstPublicDate":"2024-07-24","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2012-01-25","entryVersion":118,"sequenceVersion":2},"annotationScore":5.0,"organism":{"scientificName":"Borreliella burgdorferi (strain ATCC 35210 / DSM 4680 / CIP 102532 / B31)","commonName":"Borrelia burgdorferi","taxonId":224326,"lineage":["Bacteria","Pseudomonadati","Spirochaetota","Spirochaetia","Spirochaetales","Borreliaceae","Borreliella"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Transcriptional/translational repressor BpuR"}},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"23846702"}],"value":"Borrelia PUR domain protein"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"23974034"}],"value":"BpuR"}]},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"DNA-binding transcriptional repressor BpuR"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"RNA-binding translational repressor BpuR"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"23974034"}],"value":"bpuR"},"orderedLocusNames":[{"evidences":[{"evidenceCode":"ECO:0000312","source":"EMBL","id":"AAC66434.2"}],"value":"BB_0047"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20976240"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23846702"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"31240776"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"31240776"}],"value":"A transcription and translation regulator. Binds DNA adjacent to the erp transcriptional promoter; in combination with BpbA inhibits transcription from the promoter (PubMed:23846702). EbfC counteracts the effect of Bpur on BpbA (PubMed:23846702). Overexpression leads to decreased amounts of ErpA protein (PubMed:23846702). Binds DNA and RNA; binds preferentially to RNA (dissociation constant 13 nM), dsDNA (dissociation constant 130 nM) and least well to ssDNA (dissociation constant 390 nM) (PubMed:23846702). Binds to its own (RNA) transcript in vivo and in vitro but not to its (DNA) promoter; inhibits translation from its mRNA (PubMed:23974034). Binds mRNA for superoxide dismutase (sod, SodA) but not the sod promoter; increased expression of BpuR leads to decreased levels of SodA (PubMed:31240776). May post-translationally influence the levels of other proteins also (Probable) (PubMed:31240776). Prefers purine-rich over pyrimidine-rich nucleic acids (PubMed:20976240, PubMed:23846702). Separates the strands of dsDNA (PubMed:23846702)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"20976240"}],"value":"Homodimer (PubMed:20976240)"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23846702"}],"value":"Cytoplasm","id":"SL-0086"}}]},{"commentType":"ALTERNATIVE PRODUCTS","events":["Alternative initiation"],"isoforms":[{"name":{"value":"Short"},"isoformIds":["O51076-1"],"isoformSequenceStatus":"Displayed"},{"name":{"value":"Long"},"isoformIds":["O51076-2"],"sequenceIds":["VSP_062367"],"isoformSequenceStatus":"Described"}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23846702"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23974034"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"31240776"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"31240776"}],"value":"Highest expression occurs during conditions of slow bacterial growth and division (at protein level) (PubMed:23846702). Inhibits its own translation (PubMed:23974034). Transcript levels are high in unfed I.scapularis larvae, decrease as the tick larvae feed on mice and rise again as the larvae become nymphs; mice that been infected for 1 month have no detectable transcript (they remain infected with bacteria) (PubMed:31240776). Transcription may be under control of DnaA (Probable) (PubMed:31240776)"}],"commentType":"INDUCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23846702"}],"value":"Essential, it cannot be deleted (PubMed:23846702)"}],"commentType":"DISRUPTION PHENOTYPE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23846702"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23974034"}],"value":"Both isoforms are expressed in vivo; the experiments here were performed with the shorter protein which is shown here (PubMed:23846702, PubMed:23974034)"}],"commentType":"MISCELLANEOUS"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the PUR DNA-binding protein family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":122,"modifier":"EXACT"}},"description":"Transcriptional/translational repressor 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