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Recognizes and binds abasic sites in ssDNA at replication forks and chemically modifies the lesion by forming a covalent cross-link with DNA: forms a stable thiazolidine linkage between a ring-opened abasic site and the alpha-amino and sulfhydryl substituents of its N-terminal catalytic cysteine residue (By similarity). The DNA-protein cross-link is then reversed: able to catalyze the reversal of the thiazolidine cross-link and cycle between a cross-link and a non-cross-linked state depending on DNA context: mediates self-reversal of the thiazolidine cross-link in double stranded DNA (By similarity). May act as a protease: mediates autocatalytic processing of its N-terminal methionine in order to expose the catalytic cysteine (By similarity)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q96FZ2"}],"value":"Formation and reversal of DNA-protein cross-link depends on DNA context. Catalyzes formation of the thiazolidine linkage in presence of abasic sites in single-stranded DNA. Mediates the reversal of the thiazolidine cross-link in presence of double stranded DNA"}],"commentType":"ACTIVITY REGULATION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P76318"},{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q96FZ2"}],"value":"The N-terminal catalytic Cys-2 residue forms a thiazolidine linkage to a ring-opened DNA abasic site (By similarity). Glu-106 catalyzes reversal of the thiazolidine linkage; self-reversal is favoured by duplex DNA formation (By similarity). Glu-106 is also involved in sensing abasic sites in single-stranded DNA (ssDNA). His-163 stabilizes the abasic sites by forming a hydrogen bond with the O4' hydroxyl group (By similarity)"}],"commentType":"DOMAIN"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the SOS response-associated peptidase family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Initiator methionine","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"Removed","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8R1M0"}]},{"type":"Chain","location":{"start":{"value":2,"modifier":"EXACT"},"end":{"value":227,"modifier":"EXACT"}},"description":"Abasic site processing protein YoaM","featureId":"PRO_0000164401"},{"type":"Active site","location":{"start":{"value":2,"modifier":"EXACT"},"end":{"value":2,"modifier":"EXACT"}},"description":"Nucleophile","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q96FZ2"}]},{"type":"Active 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