{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"O34851","uniProtkbId":"LDC_BACSU","entryAudit":{"firstPublicDate":"2000-05-30","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2009-05-26","entryVersion":132,"sequenceVersion":2},"annotationScore":3.0,"organism":{"scientificName":"Bacillus subtilis (strain 168)","taxonId":224308,"lineage":["Bacteria","Bacillati","Bacillota","Bacilli","Caryophanales","Bacillaceae","Bacillus"]},"proteinExistence":"2: Evidence at transcript level","proteinDescription":{"recommendedName":{"fullName":{"value":"Probable murein peptide carboxypeptidase"},"ecNumbers":[{"value":"3.4.16.-"}]},"alternativeNames":[{"fullName":{"value":"LD-carboxypeptidase"}}]},"genes":[{"geneName":{"value":"ykfA"},"orderedLocusNames":[{"value":"BSU12970"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"May be involved in the degradation of peptidoglycan by catalyzing the cleavage of the terminal D-alanine residue from cytoplasmic murein peptides"}],"commentType":"FUNCTION"},{"texts":[{"value":"Cell wall degradation; peptidoglycan degradation"}],"commentType":"PATHWAY"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"Cytoplasm","id":"SL-0086"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15101989"}],"value":"Repressed by AbrB, a transcription factor that negatively controls biofilm formation"}],"commentType":"INDUCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the peptidase S66 family"}],"commentType":"SIMILARITY"},{"commentType":"SEQUENCE CAUTION","sequenceCautionType":"Frameshift","sequence":"CAA05577.1","evidences":[{"evidenceCode":"ECO:0000305"}]}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":319,"modifier":"EXACT"}},"description":"Probable murein peptide carboxypeptidase","featureId":"PRO_0000172841"},{"type":"Active site","location":{"start":{"value":116,"modifier":"EXACT"},"end":{"value":116,"modifier":"EXACT"}},"description":"Nucleophile","evidences":[{"evidenceCode":"ECO:0000250"}]},{"type":"Active site","location":{"start":{"value":214,"modifier":"EXACT"},"end":{"value":214,"modifier":"EXACT"}},"description":"Charge relay system","evidences":[{"evidenceCode":"ECO:0000250"}]},{"type":"Active site","location":{"start":{"value":284,"modifier":"EXACT"},"end":{"value":284,"modifier":"EXACT"}},"description":"Charge relay system","evidences":[{"evidenceCode":"ECO:0000250"}]}],"keywords":[{"id":"KW-0121","category":"Molecular function","name":"Carboxypeptidase"},{"id":"KW-0961","category":"Biological process","name":"Cell wall biogenesis/degradation"},{"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"id":"KW-0378","category":"Molecular function","name":"Hydrolase"},{"id":"KW-0645","category":"Molecular function","name":"Protease"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0720","category":"Molecular function","name":"Serine protease"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-6IV0A1CQJFG2P","citationType":"submission","authors":["Devine K.M."],"title":"Sequence of the Bacillus subtilis genome between xlyA and ykoR.","publicationDate":"NOV-1997","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"],"referenceComments":[{"value":"168","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"9384377","citationType":"journal article","authors":["Kunst F.","Ogasawara N.","Moszer I.","Albertini A.M.","Alloni G.","Azevedo V.","Bertero M.G.","Bessieres P.","Bolotin A.","Borchert S.","Borriss R.","Boursier L.","Brans A.","Braun M.","Brignell S.C.","Bron S.","Brouillet S.","Bruschi C.V.","Caldwell B.","Capuano V.","Carter N.M.","Choi S.-K.","Codani J.-J.","Connerton I.F.","Cummings N.J.","Daniel R.A.","Denizot F.","Devine K.M.","Duesterhoeft A.","Ehrlich S.D.","Emmerson P.T.","Entian K.-D.","Errington J.","Fabret C.","Ferrari E.","Foulger D.","Fritz C.","Fujita M.","Fujita Y.","Fuma S.","Galizzi A.","Galleron N.","Ghim S.-Y.","Glaser P.","Goffeau A.","Golightly E.J.","Grandi G.","Guiseppi G.","Guy B.J.","Haga K.","Haiech J.","Harwood C.R.","Henaut A.","Hilbert H.","Holsappel S.","Hosono S.","Hullo M.-F.","Itaya M.","Jones L.-M.","Joris B.","Karamata D.","Kasahara Y.","Klaerr-Blanchard M.","Klein C.","Kobayashi Y.","Koetter P.","Koningstein G.","Krogh S.","Kumano M.","Kurita K.","Lapidus A.","Lardinois S.","Lauber J.","Lazarevic V.","Lee S.-M.","Levine A.","Liu H.","Masuda S.","Mauel C.","Medigue C.","Medina N.","Mellado R.P.","Mizuno M.","Moestl D.","Nakai S.","Noback M.","Noone D.","O'Reilly M.","Ogawa K.","Ogiwara A.","Oudega B.","Park S.-H.","Parro V.","Pohl T.M.","Portetelle D.","Porwollik S.","Prescott A.M.","Presecan E.","Pujic P.","Purnelle B.","Rapoport G.","Rey M.","Reynolds S.","Rieger M.","Rivolta C.","Rocha E.","Roche B.","Rose M.","Sadaie Y.","Sato T.","Scanlan E.","Schleich S.","Schroeter R.","Scoffone F.","Sekiguchi J.","Sekowska A.","Seror S.J.","Serror P.","Shin B.-S.","Soldo B.","Sorokin A.","Tacconi E.","Takagi T.","Takahashi H.","Takemaru K.","Takeuchi M.","Tamakoshi A.","Tanaka T.","Terpstra P.","Tognoni A.","Tosato V.","Uchiyama S.","Vandenbol M.","Vannier F.","Vassarotti A.","Viari A.","Wambutt R.","Wedler E.","Wedler H.","Weitzenegger T.","Winters P.","Wipat A.","Yamamoto H.","Yamane K.","Yasumoto K.","Yata K.","Yoshida K.","Yoshikawa H.-F.","Zumstein E.","Yoshikawa H.","Danchin A."],"citationCrossReferences":[{"database":"PubMed","id":"9384377"},{"database":"DOI","id":"10.1038/36786"}],"title":"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis.","publicationDate":"1997","journal":"Nature","firstPage":"249","lastPage":"256","volume":"390"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"168","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"19383706","citationType":"journal article","authors":["Barbe V.","Cruveiller S.","Kunst F.","Lenoble P.","Meurice G.","Sekowska A.","Vallenet D.","Wang T.","Moszer I.","Medigue C.","Danchin A."],"citationCrossReferences":[{"database":"PubMed","id":"19383706"},{"database":"DOI","id":"10.1099/mic.0.027839-0"}],"title":"From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later.","publicationDate":"2009","journal":"Microbiology","firstPage":"1758","lastPage":"1775","volume":"155"},"referencePositions":["SEQUENCE REVISION TO N-TERMINUS"]},{"referenceNumber":4,"citation":{"id":"15101989","citationType":"journal article","authors":["Hamon M.A.","Stanley N.R.","Britton R.A.","Grossman A.D.","Lazazzera B.A."],"citationCrossReferences":[{"database":"PubMed","id":"15101989"},{"database":"DOI","id":"10.1111/j.1365-2958.2004.04023.x"}],"title":"Identification of AbrB-regulated genes involved in biofilm formation by Bacillus subtilis.","publicationDate":"2004","journal":"Mol. Microbiol.","firstPage":"847","lastPage":"860","volume":"52"},"referencePositions":["INDUCTION"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AJ002571","properties":[{"key":"ProteinId","value":"CAA05577.1"},{"key":"Status","value":"ALT_FRAME"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AL009126","properties":[{"key":"ProteinId","value":"CAB13154.2"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"G69855","properties":[{"key":"EntryName","value":"G69855"}]},{"database":"RefSeq","id":"WP_009967072.1","properties":[{"key":"NucleotideSequenceId","value":"NC_000964.3"}]},{"database":"AlphaFoldDB","id":"O34851","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"O34851","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"O34851","properties":[{"key":"Number of interactors","value":"159"}]},{"database":"STRING","id":"224308.BSU12970","properties":[{"key":"Description","value":"-"}]},{"database":"MEROPS","id":"S66.001","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"224308-BSU12970","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblBacteria","id":"CAB13154","properties":[{"key":"ProteinId","value":"CAB13154"},{"key":"GeneId","value":"BSU_12970"}]},{"database":"GeneID","id":"938175","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"bsu:BSU12970","properties":[{"key":"Description","value":"-"}]},{"database":"PATRIC","id":"fig|224308.179.peg.1409","properties":[{"key":"GeneDesignation","value":"-"}]},{"database":"eggNOG","id":"COG1619","properties":[{"key":"ToxonomicScope","value":"Bacteria"}]},{"database":"InParanoid","id":"O34851","properties":[{"key":"Description","value":"-"}]},{"database":"OrthoDB","id":"9807329at2","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"O34851","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"BSUB:BSU12970-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"UniPathway","id":"UPA00549","properties":[{"key":"RectionId","value":"-"}]},{"database":"Proteomes","id":"UP000001570","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"AbasyAtlas","id":"O34851","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005829","properties":[{"key":"GoTerm","value":"C:cytosol"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0004180","properties":[{"key":"GoTerm","value":"F:carboxypeptidase activity"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0016998","properties":[{"key":"GoTerm","value":"P:cell wall macromolecule catabolic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniPathway"}]},{"database":"CDD","id":"cd07025","properties":[{"key":"EntryName","value":"Peptidase_S66"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.10740","properties":[{"key":"EntryName","value":"Class I glutamine amidotransferase-like"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.50.30.60","properties":[{"key":"EntryName","value":"LD-carboxypeptidase A C-terminal domain-like"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR027461","properties":[{"key":"EntryName","value":"Carboxypeptidase_A_C_sf"}]},{"database":"InterPro","id":"IPR029062","properties":[{"key":"EntryName","value":"Class_I_gatase-like"}]},{"database":"InterPro","id":"IPR027478","properties":[{"key":"EntryName","value":"LdcA_N"}]},{"database":"InterPro","id":"IPR040449","properties":[{"key":"EntryName","value":"Peptidase_S66_N"}]},{"database":"InterPro","id":"IPR040921","properties":[{"key":"EntryName","value":"Peptidase_S66C"}]},{"database":"InterPro","id":"IPR003507","properties":[{"key":"EntryName","value":"S66_fam"}]},{"database":"PANTHER","id":"PTHR30237","properties":[{"key":"EntryName","value":"MURAMOYLTETRAPEPTIDE CARBOXYPEPTIDASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR30237:SF2","properties":[{"key":"EntryName","value":"MUREIN TETRAPEPTIDE CARBOXYPEPTIDASE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF02016","properties":[{"key":"EntryName","value":"Peptidase_S66"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF17676","properties":[{"key":"EntryName","value":"Peptidase_S66C"},{"key":"MatchStatus","value":"1"}]},{"database":"PIRSF","id":"PIRSF028757","properties":[{"key":"EntryName","value":"LD-carboxypeptidase"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF52317","properties":[{"key":"EntryName","value":"Class I glutamine amidotransferase-like"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF141986","properties":[{"key":"EntryName","value":"LD-carboxypeptidase A C-terminal domain-like"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MKGVFSLNYKPKALNKGDTVGVIAPASPPDPKKLDTALLFLEELGLQVKLGKALKNQHGYLAGQDDERLADLHEMFRDDEVKAVLCACGGFGTGRIAAGIDFSLIRKHPKIFWGYSDITFLHTAIHQNTGLVTFHGPMLSTDIGLDDVHPLTKASYKQLFQETEFTYTEELSPLTELVPGKAEGELVGGNLSLLTSTLGTPFEIDTRGKLLFIEDIDEEPYQIDRMLNQLKMGGKLTDAAGILVCDFHNCVPVKREKSLSLEQVLEDYIISAGRPALRGFKIGHCSPSIAVPIGAKAAMNTAEKTAVIEAGVSEGALKT","length":319,"molWeight":34596,"crc64":"1E328AA2BC5077EE","md5":"D70DFA1B000A94E2AF3842F9A4CE4EDE"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"PATHWAY":1,"SUBCELLULAR LOCATION":1,"INDUCTION":1,"SIMILARITY":1,"SEQUENCE CAUTION":1},"countByFeatureType":{"Chain":1,"Active site":3},"uniParcId":"UPI000195C5B8"}}