{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"O34758","uniProtkbId":"MLTG_BACSU","entryAudit":{"firstPublicDate":"2009-11-03","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"1998-01-01","entryVersion":113,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Bacillus subtilis (strain 168)","taxonId":224308,"lineage":["Bacteria","Bacillati","Bacillota","Bacilli","Caryophanales","Bacillaceae","Bacillus"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_02065"}],"value":"Endolytic murein transglycosylase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_02065"}],"value":"4.2.2.29"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_02065"}],"value":"Peptidoglycan lytic transglycosylase"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_02065"}],"value":"Peptidoglycan polymerization terminase"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_02065"}],"value":"mltG"},"synonyms":[{"value":"yrrL"}],"orderedLocusNames":[{"value":"BSU27370"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_02065"}],"value":"Functions as a peptidoglycan terminase that cleaves nascent peptidoglycan strands endolytically to terminate their elongation"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a peptidoglycan chain = a peptidoglycan chain with N-acetyl-1,6-anhydromuramyl-[peptide] at the reducing end + a peptidoglycan chain with N-acetylglucosamine at the non-reducing end.","ecNumber":"4.2.2.29","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_02065"}]}},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_02065"}],"value":"Cell membrane","id":"SL-0039"},"topology":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_02065"}],"value":"Single-pass membrane protein","id":"SL-9904"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_02065"}],"value":"Belongs to the transglycosylase MltG family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":360,"modifier":"EXACT"}},"description":"Endolytic murein transglycosylase","featureId":"PRO_0000388727"},{"type":"Transmembrane","location":{"start":{"value":16,"modifier":"EXACT"},"end":{"value":36,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_02065"}]},{"type":"Site","location":{"start":{"value":242,"modifier":"EXACT"},"end":{"value":242,"modifier":"EXACT"}},"description":"Important for catalytic activity","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_02065"}]}],"keywords":[{"id":"KW-1003","category":"Cellular component","name":"Cell membrane"},{"id":"KW-0961","category":"Biological process","name":"Cell wall biogenesis/degradation"},{"id":"KW-0456","category":"Molecular function","name":"Lyase"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"id":"KW-1133","category":"Domain","name":"Transmembrane helix"}],"references":[{"referenceNumber":1,"citation":{"id":"9384377","citationType":"journal article","authors":["Kunst F.","Ogasawara N.","Moszer I.","Albertini A.M.","Alloni G.","Azevedo V.","Bertero M.G.","Bessieres P.","Bolotin A.","Borchert S.","Borriss R.","Boursier L.","Brans A.","Braun M.","Brignell S.C.","Bron S.","Brouillet S.","Bruschi C.V.","Caldwell B.","Capuano V.","Carter N.M.","Choi S.-K.","Codani J.-J.","Connerton I.F.","Cummings N.J.","Daniel R.A.","Denizot F.","Devine K.M.","Duesterhoeft A.","Ehrlich S.D.","Emmerson P.T.","Entian K.-D.","Errington J.","Fabret C.","Ferrari E.","Foulger D.","Fritz C.","Fujita M.","Fujita Y.","Fuma S.","Galizzi A.","Galleron N.","Ghim S.-Y.","Glaser P.","Goffeau A.","Golightly E.J.","Grandi G.","Guiseppi G.","Guy B.J.","Haga K.","Haiech J.","Harwood C.R.","Henaut A.","Hilbert H.","Holsappel S.","Hosono S.","Hullo M.-F.","Itaya M.","Jones L.-M.","Joris B.","Karamata D.","Kasahara Y.","Klaerr-Blanchard M.","Klein C.","Kobayashi Y.","Koetter P.","Koningstein G.","Krogh S.","Kumano M.","Kurita K.","Lapidus A.","Lardinois S.","Lauber J.","Lazarevic V.","Lee S.-M.","Levine A.","Liu H.","Masuda S.","Mauel C.","Medigue C.","Medina N.","Mellado R.P.","Mizuno M.","Moestl D.","Nakai S.","Noback M.","Noone D.","O'Reilly M.","Ogawa K.","Ogiwara A.","Oudega B.","Park S.-H.","Parro V.","Pohl T.M.","Portetelle D.","Porwollik S.","Prescott A.M.","Presecan E.","Pujic P.","Purnelle B.","Rapoport G.","Rey M.","Reynolds S.","Rieger M.","Rivolta C.","Rocha E.","Roche B.","Rose M.","Sadaie Y.","Sato T.","Scanlan E.","Schleich S.","Schroeter R.","Scoffone F.","Sekiguchi J.","Sekowska A.","Seror S.J.","Serror P.","Shin B.-S.","Soldo B.","Sorokin A.","Tacconi E.","Takagi T.","Takahashi H.","Takemaru K.","Takeuchi M.","Tamakoshi A.","Tanaka T.","Terpstra P.","Tognoni A.","Tosato V.","Uchiyama S.","Vandenbol M.","Vannier F.","Vassarotti A.","Viari A.","Wambutt R.","Wedler E.","Wedler H.","Weitzenegger T.","Winters P.","Wipat A.","Yamamoto H.","Yamane K.","Yasumoto K.","Yata K.","Yoshida K.","Yoshikawa H.-F.","Zumstein E.","Yoshikawa H.","Danchin A."],"citationCrossReferences":[{"database":"PubMed","id":"9384377"},{"database":"DOI","id":"10.1038/36786"}],"title":"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis.","publicationDate":"1997","journal":"Nature","firstPage":"249","lastPage":"256","volume":"390"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"168","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AL009126","properties":[{"key":"ProteinId","value":"CAB14679.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"E69979","properties":[{"key":"EntryName","value":"E69979"}]},{"database":"RefSeq","id":"NP_390615.1","properties":[{"key":"NucleotideSequenceId","value":"NC_000964.3"}]},{"database":"RefSeq","id":"WP_003229799.1","properties":[{"key":"NucleotideSequenceId","value":"NC_000964.3"}]},{"database":"AlphaFoldDB","id":"O34758","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"O34758","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"O34758","properties":[{"key":"Number of interactors","value":"467"}]},{"database":"STRING","id":"224308.BSU27370","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"224308-BSU27370","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblBacteria","id":"CAB14679","properties":[{"key":"ProteinId","value":"CAB14679"},{"key":"GeneId","value":"BSU_27370"}]},{"database":"GeneID","id":"936260","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"bsu:BSU27370","properties":[{"key":"Description","value":"-"}]},{"database":"PATRIC","id":"fig|224308.179.peg.2973","properties":[{"key":"GeneDesignation","value":"-"}]},{"database":"eggNOG","id":"COG1559","properties":[{"key":"ToxonomicScope","value":"Bacteria"}]},{"database":"InParanoid","id":"O34758","properties":[{"key":"Description","value":"-"}]},{"database":"OrthoDB","id":"9814591at2","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"O34758","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"BSUB:BSU27370-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000001570","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"AbasyAtlas","id":"O34758","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005886","properties":[{"key":"GoTerm","value":"C:plasma membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0008932","properties":[{"key":"GoTerm","value":"F:lytic endotransglycosylase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0009252","properties":[{"key":"GoTerm","value":"P:peptidoglycan biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"CDD","id":"cd08010","properties":[{"key":"EntryName","value":"MltG_like"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.30.160.60:FF:000242","properties":[{"key":"EntryName","value":"Endolytic murein transglycosylase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.160.60","properties":[{"key":"EntryName","value":"Classic Zinc Finger"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.1490.480","properties":[{"key":"EntryName","value":"Endolytic murein transglycosylase"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_02065","properties":[{"key":"EntryName","value":"MltG"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR003770","properties":[{"key":"EntryName","value":"MLTG-like"}]},{"database":"NCBIfam","id":"TIGR00247","properties":[{"key":"EntryName","value":"endolytic transglycosylase MltG"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR30518","properties":[{"key":"EntryName","value":"ENDOLYTIC MUREIN TRANSGLYCOSYLASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR30518:SF2","properties":[{"key":"EntryName","value":"ENDOLYTIC MUREIN TRANSGLYCOSYLASE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF02618","properties":[{"key":"EntryName","value":"YceG"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MYINQQKKSFFNKKRIILSSIVVLFLIIGGAFLYGKSLLEPVEKDSKTTVNINIPSGSSVSAIASILKKNDVIKSEKAFQYYVKYKGASGFQAGFYHLNKGMDLDAIIQKLTSGATGYAFQITVTEGAQLTQIAAAIADETKYSKKQVIAKLDDETFINQLKKEFPDTVTNDVFNKNIKHPLEGYLFPATYPFNDPDTSLEDIIKAMIKQTNSYVETYKSEMKKNKVSVHKLLTMASLIEEEATEKADRHKIASVFYNRLKKKMPLQTDPTVLYAAGKHKDRVLYKDLEIDSPYNTYKNTGLTPGPIANAGMSSWEAALHPDKTDYLYFLAKSNGEVVFTKTLKEHNKAKEKYISSKNEK","length":360,"molWeight":40502,"crc64":"4EFDC5CC9B0588C3","md5":"99F68B2A4796D02F0FD090081EDF5422"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Transmembrane":1,"Site":1},"uniParcId":"UPI0000060814"}}