{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"O34530","uniProtkbId":"RSGA_BACSU","entryAudit":{"firstPublicDate":"2003-10-31","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"1998-01-01","entryVersion":149,"sequenceVersion":1},"annotationScore":5.0,"organism":{"scientificName":"Bacillus subtilis (strain 168)","taxonId":224308,"lineage":["Bacteria","Bacillati","Bacillota","Bacilli","Caryophanales","Bacillaceae","Bacillus"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01820"}],"value":"Small ribosomal subunit biogenesis GTPase RsgA"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01820"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16485133"}],"value":"3.6.1.-"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"22544754"}],"value":"Ribosome-associated GTPase CpgA"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01820"}],"value":"rsgA"},"synonyms":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"16485133"}],"value":"cpgA"},{"value":"engC"},{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"9534248"}],"value":"yloQ"}],"orderedLocusNames":[{"value":"BSU15780"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15828870"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16485133"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18344364"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22544754"}],"value":"One of several proteins that assist in the late maturation steps of the functional core of the 30S ribosomal subunit. Helps release RbfA from mature subunits. May play a role in the assembly of ribosomal proteins into the subunit. Circularly permuted GTPase with a low level of activity and slow catalytic turnover, does not act on ATP (PubMed:16485133). GTPase activity is stimulated by the presence of 30S or 70S ribosomes, phosphorylation increases stimulation (PubMed:22544754). Depletion results in increased sensitivity to protein synthesis inhibitors that block the peptide channel or peptidyl transferase center on the ribosome, suggesting this protein functions in conjunction with the ribosome in vivo (PubMed:15828870). Decreasing levels of protein lead to an increase in free 30S and 50S ribosomal subunits and a decrease in assembled 70S ribosomes (PubMed:15828870). Suggested to serve as a specific transcription factor for proteins involved in late stages of peptidoglycan synthesis (PubMed:18344364)"}],"commentType":"FUNCTION"},{"commentType":"COFACTOR","cofactors":[{"name":"Zn(2+)","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01820"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15223319"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:29105"}}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01820"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15223319"}],"value":"Binds 1 zinc ion per subunit."}]}},{"commentType":"BIOPHYSICOCHEMICAL PROPERTIES","kineticParameters":{"michaelisConstants":[{"constant":30.5,"unit":"uM","substrate":"GTP","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16485133"}]}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16485133"}],"value":"kcat for GTP is 13.6 h(-1), in the absence of ribosomes."}]}}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16485133"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22544754"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"15223319"}],"value":"Monomer, but able to form dimers (PubMed:16485133). Associates with 30S ribosomal subunit; a phospho-mimetic mutation increases association (PubMed:22544754). Probably binds 16S rRNA"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01820"}],"value":"Cytoplasm","id":"SL-0086"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"19246764"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22544754"}],"value":"In vitro phosphorylated mostly on Thr (with lower signal on Ser) by PrkC in the presence of poly-L-Lys or myelin basic protein, dephosphorylated by PrpC (PubMed:19246764). Most in vitro phosphorylation occurs on Thr-166, in vivo phosphorylation has not been detected, but it might vary during the cell cycle (PubMed:22544754)"}],"commentType":"PTM"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15828870"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16485133"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18344364"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9743119"}],"value":"Has been described as essential (PubMed:9743119, PubMed:16485133, PubMed:18344364), but also as non-essential (PubMed:15828870). Cells have a slow growth phenotype (PubMed:15828870, PubMed:16485133, PubMed:18344364, PubMed:22544754). Disrupted strain grows as chains of filaments, a cell curvature phenotype is also present, resulting in long wavy cells or short curved rods (PubMed:15828870, PubMed:22544754). Depleted cells form aberrant, swollen cells (PubMed:16485133, PubMed:18344364). Depleted cells DNA staining shows fragmented and/or disturbed nucleoid segregation; effects are seen most in minimal E-medium (PubMed:16485133). Depleted cells have an irregular deposition of cell wall and 15% have abnormal septal cleavage planes (PubMed:18344364)"}],"commentType":"DISRUPTION PHENOTYPE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01820"},{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the TRAFAC class YlqF/YawG GTPase family. RsgA subfamily"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":298,"modifier":"EXACT"}},"description":"Small ribosomal subunit biogenesis GTPase RsgA","featureId":"PRO_0000171464"},{"type":"Domain","location":{"start":{"value":67,"modifier":"EXACT"},"end":{"value":228,"modifier":"EXACT"}},"description":"CP-type G","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01058"}]},{"type":"Binding site","location":{"start":{"value":116,"modifier":"EXACT"},"end":{"value":119,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:37565"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01820"}],"ligand":{"name":"GTP","id":"ChEBI:CHEBI:37565"}},{"type":"Binding site","location":{"start":{"value":171,"modifier":"EXACT"},"end":{"value":179,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:37565"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01820"}],"ligand":{"name":"GTP","id":"ChEBI:CHEBI:37565"}},{"type":"Binding site","location":{"start":{"value":252,"modifier":"EXACT"},"end":{"value":252,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29105"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01820"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15223319"}],"ligand":{"name":"Zn(2+)","id":"ChEBI:CHEBI:29105"}},{"type":"Binding site","location":{"start":{"value":257,"modifier":"EXACT"},"end":{"value":257,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29105"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01820"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15223319"}],"ligand":{"name":"Zn(2+)","id":"ChEBI:CHEBI:29105"}},{"type":"Binding site","location":{"start":{"value":259,"modifier":"EXACT"},"end":{"value":259,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29105"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01820"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15223319"}],"ligand":{"name":"Zn(2+)","id":"ChEBI:CHEBI:29105"}},{"type":"Binding site","location":{"start":{"value":265,"modifier":"EXACT"},"end":{"value":265,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29105"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01820"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15223319"}],"ligand":{"name":"Zn(2+)","id":"ChEBI:CHEBI:29105"}},{"type":"Modified residue","location":{"start":{"value":166,"modifier":"EXACT"},"end":{"value":166,"modifier":"EXACT"}},"description":"Phosphothreonine","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22544754"}]},{"type":"Mutagenesis","location":{"start":{"value":166,"modifier":"EXACT"},"end":{"value":166,"modifier":"EXACT"}},"description":"Loss of most phosphorylation by PrkC, GTPase activity less stimulated by 70S ribosomes than wild-type, decreased association with 30S ribosomal subunit in vitro. Grows slowly, cells have abnormal morphology.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22544754"}],"alternativeSequence":{"originalSequence":"T","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":166,"modifier":"EXACT"},"end":{"value":166,"modifier":"EXACT"}},"description":"Higher endogenous GTPase activity, GTPase more stimulated by 70S ribosomes than wild-type, increased association with 30S ribosomal subunit in vitro, wild-type behavior in vivo.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22544754"}],"alternativeSequence":{"originalSequence":"T","alternativeSequences":["D"]}},{"type":"Mutagenesis","location":{"start":{"value":177,"modifier":"EXACT"},"end":{"value":177,"modifier":"EXACT"}},"description":"Grows slowly, cells have abnormal morphology.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22544754"}],"alternativeSequence":{"originalSequence":"K","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":178,"modifier":"EXACT"},"end":{"value":178,"modifier":"EXACT"}},"description":"No GTPase activity. Wild-type phosphorylation by PrkC in vitro.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16485133"}],"alternativeSequence":{"originalSequence":"S","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":192,"modifier":"EXACT"},"end":{"value":192,"modifier":"EXACT"}},"description":"Loss of phosphorylation by PrkC, but PrkC autophosphorylation greatly decreased in vitro.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"19246764"}],"alternativeSequence":{"originalSequence":"T","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":196,"modifier":"EXACT"},"end":{"value":196,"modifier":"EXACT"}},"description":"Wild-type phosphorylation by PrkC in vitro.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"19246764"}],"alternativeSequence":{"originalSequence":"S","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":206,"modifier":"EXACT"},"end":{"value":206,"modifier":"EXACT"}},"description":"Wild-type phosphorylation by PrkC in vitro.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"19246764"}],"alternativeSequence":{"originalSequence":"T","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":222,"modifier":"EXACT"},"end":{"value":222,"modifier":"EXACT"}},"description":"Wild-type phosphorylation by PrkC in vitro.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"19246764"}],"alternativeSequence":{"originalSequence":"T","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":226,"modifier":"EXACT"},"end":{"value":226,"modifier":"EXACT"}},"description":"Loss of phosphorylation by PrkC, but PrkC autophosphorylation considerably decreased in vitro.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"19246764"}],"alternativeSequence":{"originalSequence":"S","alternativeSequences":["A"]}},{"type":"Beta strand","location":{"start":{"value":3,"modifier":"EXACT"},"end":{"value":10,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1T9H"}]},{"type":"Beta strand","location":{"start":{"value":13,"modifier":"EXACT"},"end":{"value":18,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1T9H"}]},{"type":"Beta strand","location":{"start":{"value":20,"modifier":"EXACT"},"end":{"value":23,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1T9H"}]},{"type":"Beta strand","location":{"start":{"value":25,"modifier":"EXACT"},"end":{"value":31,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1T9H"}]},{"type":"Helix","location":{"start":{"value":40,"modifier":"EXACT"},"end":{"value":42,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1T9H"}]},{"type":"Beta strand","location":{"start":{"value":47,"modifier":"EXACT"},"end":{"value":51,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1T9H"}]},{"type":"Beta strand","location":{"start":{"value":58,"modifier":"EXACT"},"end":{"value":63,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1T9H"}]},{"type":"Helix","location":{"start":{"value":64,"modifier":"EXACT"},"end":{"value":66,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1T9H"}]},{"type":"Turn","location":{"start":{"value":71,"modifier":"EXACT"},"end":{"value":74,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1T9H"}]},{"type":"Beta strand","location":{"start":{"value":80,"modifier":"EXACT"},"end":{"value":87,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1T9H"}]},{"type":"Turn","location":{"start":{"value":88,"modifier":"EXACT"},"end":{"value":91,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1T9H"}]},{"type":"Helix","location":{"start":{"value":94,"modifier":"EXACT"},"end":{"value":105,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1T9H"}]},{"type":"Turn","location":{"start":{"value":106,"modifier":"EXACT"},"end":{"value":108,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1T9H"}]},{"type":"Beta strand","location":{"start":{"value":110,"modifier":"EXACT"},"end":{"value":116,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1T9H"}]},{"type":"Helix","location":{"start":{"value":118,"modifier":"EXACT"},"end":{"value":120,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1T9H"}]},{"type":"Helix","location":{"start":{"value":124,"modifier":"EXACT"},"end":{"value":140,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1T9H"}]},{"type":"Beta strand","location":{"start":{"value":144,"modifier":"EXACT"},"end":{"value":146,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1T9H"}]},{"type":"Helix","location":{"start":{"value":149,"modifier":"EXACT"},"end":{"value":152,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1T9H"}]},{"type":"Turn","location":{"start":{"value":156,"modifier":"EXACT"},"end":{"value":158,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1T9H"}]},{"type":"Helix","location":{"start":{"value":159,"modifier":"EXACT"},"end":{"value":162,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1T9H"}]},{"type":"Beta strand","location":{"start":{"value":165,"modifier":"EXACT"},"end":{"value":172,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1T9H"}]},{"type":"Helix","location":{"start":{"value":173,"modifier":"EXACT"},"end":{"value":184,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1T9H"}]},{"type":"Helix","location":{"start":{"value":207,"modifier":"EXACT"},"end":{"value":209,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1T9H"}]},{"type":"Beta strand","location":{"start":{"value":211,"modifier":"EXACT"},"end":{"value":214,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1T9H"}]},{"type":"Beta strand","location":{"start":{"value":217,"modifier":"EXACT"},"end":{"value":222,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1T9H"}]},{"type":"Helix","location":{"start":{"value":235,"modifier":"EXACT"},"end":{"value":238,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1T9H"}]},{"type":"Helix","location":{"start":{"value":239,"modifier":"EXACT"},"end":{"value":241,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1T9H"}]},{"type":"Helix","location":{"start":{"value":243,"modifier":"EXACT"},"end":{"value":248,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1T9H"}]},{"type":"Helix","location":{"start":{"value":249,"modifier":"EXACT"},"end":{"value":251,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1T9H"}]},{"type":"Beta strand","location":{"start":{"value":259,"modifier":"EXACT"},"end":{"value":261,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1T9H"}]},{"type":"Helix","location":{"start":{"value":266,"modifier":"EXACT"},"end":{"value":273,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1T9H"}]},{"type":"Helix","location":{"start":{"value":278,"modifier":"EXACT"},"end":{"value":292,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1T9H"}]}],"keywords":[{"id":"KW-0002","category":"Technical term","name":"3D-structure"},{"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"id":"KW-0342","category":"Ligand","name":"GTP-binding"},{"id":"KW-0378","category":"Molecular function","name":"Hydrolase"},{"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"id":"KW-0547","category":"Ligand","name":"Nucleotide-binding"},{"id":"KW-0597","category":"PTM","name":"Phosphoprotein"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0690","category":"Biological process","name":"Ribosome biogenesis"},{"id":"KW-0694","category":"Molecular function","name":"RNA-binding"},{"id":"KW-0699","category":"Molecular function","name":"rRNA-binding"},{"id":"KW-0862","category":"Ligand","name":"Zinc"}],"references":[{"referenceNumber":1,"citation":{"id":"9534248","citationType":"journal article","authors":["Foulger D.","Errington J."],"citationCrossReferences":[{"database":"PubMed","id":"9534248"},{"database":"DOI","id":"10.1099/00221287-144-3-801"}],"title":"A 28 kbp segment from the spoVM region of the Bacillus subtilis 168 genome.","publicationDate":"1998","journal":"Microbiology","firstPage":"801","lastPage":"805","volume":"144"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"],"referenceComments":[{"value":"168","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"9384377","citationType":"journal article","authors":["Kunst F.","Ogasawara N.","Moszer I.","Albertini A.M.","Alloni G.","Azevedo V.","Bertero M.G.","Bessieres P.","Bolotin A.","Borchert S.","Borriss R.","Boursier L.","Brans A.","Braun M.","Brignell S.C.","Bron S.","Brouillet S.","Bruschi C.V.","Caldwell B.","Capuano V.","Carter N.M.","Choi S.-K.","Codani J.-J.","Connerton I.F.","Cummings N.J.","Daniel R.A.","Denizot F.","Devine K.M.","Duesterhoeft A.","Ehrlich S.D.","Emmerson P.T.","Entian K.-D.","Errington J.","Fabret C.","Ferrari E.","Foulger D.","Fritz C.","Fujita M.","Fujita Y.","Fuma S.","Galizzi A.","Galleron N.","Ghim S.-Y.","Glaser P.","Goffeau A.","Golightly E.J.","Grandi G.","Guiseppi G.","Guy B.J.","Haga K.","Haiech J.","Harwood C.R.","Henaut A.","Hilbert H.","Holsappel S.","Hosono S.","Hullo M.-F.","Itaya M.","Jones L.-M.","Joris B.","Karamata D.","Kasahara Y.","Klaerr-Blanchard M.","Klein C.","Kobayashi Y.","Koetter P.","Koningstein G.","Krogh S.","Kumano M.","Kurita K.","Lapidus A.","Lardinois S.","Lauber J.","Lazarevic V.","Lee S.-M.","Levine A.","Liu H.","Masuda S.","Mauel C.","Medigue C.","Medina N.","Mellado R.P.","Mizuno M.","Moestl D.","Nakai S.","Noback M.","Noone D.","O'Reilly M.","Ogawa K.","Ogiwara A.","Oudega B.","Park S.-H.","Parro V.","Pohl T.M.","Portetelle D.","Porwollik S.","Prescott A.M.","Presecan E.","Pujic P.","Purnelle B.","Rapoport G.","Rey M.","Reynolds S.","Rieger M.","Rivolta C.","Rocha E.","Roche B.","Rose M.","Sadaie Y.","Sato T.","Scanlan E.","Schleich S.","Schroeter R.","Scoffone F.","Sekiguchi J.","Sekowska A.","Seror S.J.","Serror P.","Shin B.-S.","Soldo B.","Sorokin A.","Tacconi E.","Takagi T.","Takahashi H.","Takemaru K.","Takeuchi M.","Tamakoshi A.","Tanaka T.","Terpstra P.","Tognoni A.","Tosato V.","Uchiyama S.","Vandenbol M.","Vannier F.","Vassarotti A.","Viari A.","Wambutt R.","Wedler E.","Wedler H.","Weitzenegger T.","Winters P.","Wipat A.","Yamamoto H.","Yamane K.","Yasumoto K.","Yata K.","Yoshida K.","Yoshikawa H.-F.","Zumstein E.","Yoshikawa H.","Danchin A."],"citationCrossReferences":[{"database":"PubMed","id":"9384377"},{"database":"DOI","id":"10.1038/36786"}],"title":"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis.","publicationDate":"1997","journal":"Nature","firstPage":"249","lastPage":"256","volume":"390"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"168","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"9743119","citationType":"journal article","authors":["Arigoni F.","Talabot F.","Peitsch M.C.","Edgerton M.D.","Meldrum E.","Allet E.","Fish R.","Jamotte T.","Curchod M.-L.","Loferer H."],"citationCrossReferences":[{"database":"PubMed","id":"9743119"},{"database":"DOI","id":"10.1038/nbt0998-851"}],"title":"A genome-based approach for the identification of essential bacterial genes.","publicationDate":"1998","journal":"Nat. Biotechnol.","firstPage":"851","lastPage":"856","volume":"16"},"referencePositions":["IDENTIFICATION","DISRUPTION PHENOTYPE"]},{"referenceNumber":4,"citation":{"id":"15828870","citationType":"journal article","authors":["Campbell T.L.","Daigle D.M.","Brown E.D."],"citationCrossReferences":[{"database":"PubMed","id":"15828870"},{"database":"DOI","id":"10.1042/bj20041873"}],"title":"Characterization of the Bacillus subtilis GTPase YloQ and its role in ribosome function.","publicationDate":"2005","journal":"Biochem. J.","firstPage":"843","lastPage":"852","volume":"389"},"referencePositions":["FUNCTION","DISRUPTION PHENOTYPE","CHEMICAL SYNTHETIC LETHALITY"],"referenceComments":[{"value":"168 / EB6","type":"STRAIN"}]},{"referenceNumber":5,"citation":{"id":"16485133","citationType":"journal article","authors":["Cladiere L.","Hamze K.","Madec E.","Levdikov V.M.","Wilkinson A.J.","Holland I.B.","Seror S.J."],"citationCrossReferences":[{"database":"PubMed","id":"16485133"},{"database":"DOI","id":"10.1007/s00438-006-0097-9"}],"title":"The GTPase, CpgA(YloQ), a putative translation factor, is implicated in morphogenesis in Bacillus subtilis.","publicationDate":"2006","journal":"Mol. Genet. Genomics","firstPage":"409","lastPage":"420","volume":"275"},"referencePositions":["FUNCTION AS A GTPASE","BIOPHYSICOCHEMICAL PROPERTIES","SUBUNIT","DISRUPTION PHENOTYPE","MUTAGENESIS OF SER-178"],"referenceComments":[{"value":"168","type":"STRAIN"}]},{"referenceNumber":6,"citation":{"id":"18344364","citationType":"journal article","authors":["Absalon C.","Hamze K.","Blanot D.","Frehel C.","Carballido-Lopez R.","Holland B.I.","van Heijenoort J.","Seror S.J."],"citationCrossReferences":[{"database":"PubMed","id":"18344364"},{"database":"DOI","id":"10.1128/jb.01994-07"}],"title":"The GTPase CpgA is implicated in the deposition of the peptidoglycan sacculus in Bacillus subtilis.","publicationDate":"2008","journal":"J. Bacteriol.","firstPage":"3786","lastPage":"3790","volume":"190"},"referencePositions":["FUNCTION","DISRUPTION PHENOTYPE"],"referenceComments":[{"value":"168","type":"STRAIN"}]},{"referenceNumber":7,"citation":{"id":"19246764","citationType":"journal article","authors":["Absalon C.","Obuchowski M.","Madec E.","Delattre D.","Holland I.B.","Seror S.J."],"citationCrossReferences":[{"database":"PubMed","id":"19246764"},{"database":"DOI","id":"10.1099/mic.0.022475-0"}],"title":"CpgA, EF-Tu and the stressosome protein YezB are substrates of the Ser/Thr kinase/phosphatase couple, PrkC/PrpC, in Bacillus subtilis.","publicationDate":"2009","journal":"Microbiology","firstPage":"932","lastPage":"943","volume":"155"},"referencePositions":["PHOSPHORYLATION AT SER AND THR","MUTAGENESIS OF SER-178; THR-192; SER-196; THR-206; THR-222 AND SER-226"],"referenceComments":[{"value":"168","type":"STRAIN"}]},{"referenceNumber":8,"citation":{"id":"22544754","citationType":"journal article","authors":["Pompeo F.","Freton C.","Wicker-Planquart C.","Grangeasse C.","Jault J.M.","Galinier A."],"citationCrossReferences":[{"database":"PubMed","id":"22544754"},{"database":"DOI","id":"10.1074/jbc.m112.340331"}],"title":"Phosphorylation of CpgA protein enhances both its GTPase activity and its affinity for ribosome and is crucial for Bacillus subtilis growth and morphology.","publicationDate":"2012","journal":"J. Biol. Chem.","firstPage":"20830","lastPage":"20838","volume":"287"},"referencePositions":["FUNCTION","SUBUNIT","PHOSPHORYLATION AT THR-166","DISRUPTION PHENOTYPE","MUTAGENESIS OF THR-166 AND LYS-177"],"referenceComments":[{"value":"168","type":"STRAIN"}]},{"referenceNumber":9,"citation":{"id":"15223319","citationType":"journal article","authors":["Levdikov V.M.","Blagova E.V.","Brannigan J.A.","Cladiere L.","Antson A.A.","Isupov M.N.","Seror S.J.","Wilkinson A.J."],"citationCrossReferences":[{"database":"PubMed","id":"15223319"},{"database":"DOI","id":"10.1016/j.jmb.2004.05.029"}],"title":"The crystal structure of YloQ, a circularly permuted GTPase essential for Bacillus subtilis viability.","publicationDate":"2004","journal":"J. Mol. Biol.","firstPage":"767","lastPage":"782","volume":"340"},"referencePositions":["X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS) IN COMPLEX WITH ZINC IONS","PROBABLE SUBUNIT"],"evidences":[{"evidenceCode":"ECO:0007744","source":"PDB","id":"1T9H"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"Y13937","properties":[{"key":"ProteinId","value":"CAA74251.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AL009126","properties":[{"key":"ProteinId","value":"CAB13451.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"A69879","properties":[{"key":"EntryName","value":"A69879"}]},{"database":"RefSeq","id":"NP_389460.1","properties":[{"key":"NucleotideSequenceId","value":"NC_000964.3"}]},{"database":"RefSeq","id":"WP_003232060.1","properties":[{"key":"NucleotideSequenceId","value":"NC_000964.3"}]},{"database":"PDB","id":"1T9H","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.60 A"},{"key":"Chains","value":"A=1-298"}]},{"database":"PDBsum","id":"1T9H","properties":[{"key":"Description","value":"-"}]},{"database":"AlphaFoldDB","id":"O34530","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"O34530","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"O34530","properties":[{"key":"Number of interactors","value":"342"}]},{"database":"STRING","id":"224308.BSU15780","properties":[{"key":"Description","value":"-"}]},{"database":"iPTMnet","id":"O34530","properties":[{"key":"Description","value":"-"}]},{"database":"jPOST","id":"O34530","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"224308-BSU15780","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblBacteria","id":"CAB13451","properties":[{"key":"ProteinId","value":"CAB13451"},{"key":"GeneId","value":"BSU_15780"}]},{"database":"GeneID","id":"938451","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"bsu:BSU15780","properties":[{"key":"Description","value":"-"}]},{"database":"PATRIC","id":"fig|224308.179.peg.1718","properties":[{"key":"GeneDesignation","value":"-"}]},{"database":"eggNOG","id":"COG1162","properties":[{"key":"ToxonomicScope","value":"Bacteria"}]},{"database":"InParanoid","id":"O34530","properties":[{"key":"Description","value":"-"}]},{"database":"OrthoDB","id":"9809485at2","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"O34530","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"BSUB:BSU15780-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"EvolutionaryTrace","id":"O34530","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000001570","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0005525","properties":[{"key":"GoTerm","value":"F:GTP binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0003924","properties":[{"key":"GoTerm","value":"F:GTPase activity"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0019843","properties":[{"key":"GoTerm","value":"F:rRNA binding"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0000028","properties":[{"key":"GoTerm","value":"P:ribosomal small subunit assembly"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"CDD","id":"cd04466","properties":[{"key":"EntryName","value":"S1_YloQ_GTPase"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd01854","properties":[{"key":"EntryName","value":"YjeQ_EngC"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.40.50.140","properties":[{"key":"EntryName","value":"Nucleic acid-binding proteins"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.300","properties":[{"key":"EntryName","value":"P-loop containing nucleotide triphosphate hydrolases"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.40.50","properties":[{"key":"EntryName","value":"Probable gtpase engc, domain 3"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_01820","properties":[{"key":"EntryName","value":"GTPase_RsgA"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR030378","properties":[{"key":"EntryName","value":"G_CP_dom"}]},{"database":"InterPro","id":"IPR012340","properties":[{"key":"EntryName","value":"NA-bd_OB-fold"}]},{"database":"InterPro","id":"IPR027417","properties":[{"key":"EntryName","value":"P-loop_NTPase"}]},{"database":"InterPro","id":"IPR004881","properties":[{"key":"EntryName","value":"Ribosome_biogen_GTPase_RsgA"}]},{"database":"InterPro","id":"IPR010914","properties":[{"key":"EntryName","value":"RsgA_GTPase_dom"}]},{"database":"InterPro","id":"IPR031944","properties":[{"key":"EntryName","value":"RsgA_N"}]},{"database":"NCBIfam","id":"TIGR00157","properties":[{"key":"EntryName","value":"ribosome small subunit-dependent GTPase A"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR32120","properties":[{"key":"EntryName","value":"SMALL RIBOSOMAL SUBUNIT BIOGENESIS GTPASE RSGA"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR32120:SF11","properties":[{"key":"EntryName","value":"SMALL RIBOSOMAL SUBUNIT BIOGENESIS GTPASE RSGA 1, MITOCHONDRIAL-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF03193","properties":[{"key":"EntryName","value":"RsgA_GTPase"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF16745","properties":[{"key":"EntryName","value":"RsgA_N"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF50249","properties":[{"key":"EntryName","value":"Nucleic acid-binding proteins"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF52540","properties":[{"key":"EntryName","value":"P-loop containing nucleoside triphosphate hydrolases"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS50936","properties":[{"key":"EntryName","value":"ENGC_GTPASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51721","properties":[{"key":"EntryName","value":"G_CP"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MPEGKIIKALSGFYYVLDESEDSDKVIQCRGRGIFRKNKITPLVGDYVVYQAENDKEGYLMEIKERTNELIRPPICNVDQAVLVFSAVQPSFSTALLDRFLVLVEANDIQPIICITKMDLIEDQDTEDTIQAYAEDYRNIGYDVYLTSSKDQDSLADIIPHFQDKTTVFAGQSGVGKSSLLNAISPELGLRTNEISEHLGRGKHTTRHVELIHTSGGLVADTPGFSSLEFTDIEEEELGYTFPDIREKSSSCKFRGCLHLKEPKCAVKQAVEDGELKQYRYDHYVEFMTEIKDRKPRY","length":298,"molWeight":33797,"crc64":"E58A69CAE570F855","md5":"AFA975A2DE53F55DCB6B61A5A386EA9B"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"COFACTOR":1,"BIOPHYSICOCHEMICAL PROPERTIES":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"PTM":1,"DISRUPTION PHENOTYPE":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Domain":1,"Binding site":6,"Modified residue":1,"Mutagenesis":9,"Beta strand":13,"Helix":15,"Turn":4},"uniParcId":"UPI00000322B0"}}