{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"O34513","secondaryAccessions":["Q797S9"],"uniProtkbId":"TMCAL_BACSU","entryAudit":{"firstPublicDate":"2005-08-30","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"1998-01-01","entryVersion":111,"sequenceVersion":1},"annotationScore":5.0,"organism":{"scientificName":"Bacillus subtilis (strain 168)","taxonId":224308,"lineage":["Bacteria","Bacillati","Bacillota","Bacilli","Caryophanales","Bacillaceae","Bacillus"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01539"},{"evidenceCode":"ECO:0000303","source":"PubMed","id":"30150682"}],"value":"tRNA(Met) cytidine acetate ligase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01539"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30150682"}],"value":"6.3.1.22"}]}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01539"},{"evidenceCode":"ECO:0000303","source":"PubMed","id":"30150682"}],"value":"tmcAL"},"synonyms":[{"value":"ylbM"}],"orderedLocusNames":[{"value":"BSU15060"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01539"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30150682"}],"value":"Catalyzes the formation of N(4)-acetylcytidine (ac(4)C) at the wobble position of elongator tRNA(Met), using acetate and ATP as substrates. First activates an acetate ion to form acetyladenylate (Ac-AMP) and then transfers the acetyl group to tRNA to form ac(4)C34"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"cytidine(34) in elongator tRNA(Met) + acetate + ATP = N(4)-acetylcytidine(34) in elongator tRNA(Met) + AMP + diphosphate","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:58144"},{"database":"Rhea","id":"RHEA-COMP:10693"},{"database":"Rhea","id":"RHEA-COMP:10694"},{"database":"ChEBI","id":"CHEBI:30089"},{"database":"ChEBI","id":"CHEBI:30616"},{"database":"ChEBI","id":"CHEBI:33019"},{"database":"ChEBI","id":"CHEBI:74900"},{"database":"ChEBI","id":"CHEBI:82748"},{"database":"ChEBI","id":"CHEBI:456215"}],"ecNumber":"6.3.1.22","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01539"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30150682"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:58145"},"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01539"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30150682"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"acetate + ATP + H(+) = acetyl-AMP + diphosphate","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:58136"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:30089"},{"database":"ChEBI","id":"CHEBI:30616"},{"database":"ChEBI","id":"CHEBI:33019"},{"database":"ChEBI","id":"CHEBI:142453"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01539"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30150682"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:58137"},"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01539"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30150682"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"acetyl-AMP + cytidine(34) in elongator tRNA(Met) = N(4)-acetylcytidine(34) in elongator tRNA(Met) + AMP + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:58140"},{"database":"Rhea","id":"RHEA-COMP:10693"},{"database":"Rhea","id":"RHEA-COMP:10694"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:74900"},{"database":"ChEBI","id":"CHEBI:82748"},{"database":"ChEBI","id":"CHEBI:142453"},{"database":"ChEBI","id":"CHEBI:456215"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01539"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30150682"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:58141"},"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01539"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30150682"}]}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30150682"}],"value":"Homodimer"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01539"},{"evidenceCode":"ECO:0000305"}],"value":"Cytoplasm","id":"SL-0086"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30150682"}],"value":"Deletion mutant lacks the ac(4)C34 modification and displays a cold-sensitive phenotype"}],"commentType":"DISRUPTION PHENOTYPE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01539"},{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the TmcAL family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":415,"modifier":"EXACT"}},"description":"tRNA(Met) cytidine acetate ligase","featureId":"PRO_0000147160"},{"type":"Binding site","location":{"start":{"value":7,"modifier":"EXACT"},"end":{"value":20,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01539"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30150682"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}},{"type":"Binding site","location":{"start":{"value":101,"modifier":"EXACT"},"end":{"value":101,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01539"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30150682"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}},{"type":"Binding site","location":{"start":{"value":162,"modifier":"EXACT"},"end":{"value":162,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01539"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30150682"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}},{"type":"Binding site","location":{"start":{"value":187,"modifier":"EXACT"},"end":{"value":188,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01539"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30150682"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}},{"type":"Mutagenesis","location":{"start":{"value":9,"modifier":"EXACT"},"end":{"value":9,"modifier":"EXACT"}},"description":"Almost loss of activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30150682"}],"alternativeSequence":{"originalSequence":"E","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":14,"modifier":"EXACT"},"end":{"value":14,"modifier":"EXACT"}},"description":"Strong decrease in activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30150682"}],"alternativeSequence":{"originalSequence":"H","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":17,"modifier":"EXACT"},"end":{"value":17,"modifier":"EXACT"}},"description":"Almost loss of activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30150682"}],"alternativeSequence":{"originalSequence":"H","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":20,"modifier":"EXACT"},"end":{"value":20,"modifier":"EXACT"}},"description":"Decrease in activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30150682"}],"alternativeSequence":{"originalSequence":"H","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":44,"modifier":"EXACT"},"end":{"value":44,"modifier":"EXACT"}},"description":"Almost loss of activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30150682"}],"alternativeSequence":{"originalSequence":"R","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":129,"modifier":"EXACT"},"end":{"value":129,"modifier":"EXACT"}},"description":"Decrease in activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30150682"}],"alternativeSequence":{"originalSequence":"K","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":133,"modifier":"EXACT"},"end":{"value":133,"modifier":"EXACT"}},"description":"No change in activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30150682"}],"alternativeSequence":{"originalSequence":"K","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":134,"modifier":"EXACT"},"end":{"value":134,"modifier":"EXACT"}},"description":"No change in activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30150682"}],"alternativeSequence":{"originalSequence":"K","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":162,"modifier":"EXACT"},"end":{"value":162,"modifier":"EXACT"}},"description":"Almost loss of activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30150682"}],"alternativeSequence":{"originalSequence":"N","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":187,"modifier":"EXACT"},"end":{"value":187,"modifier":"EXACT"}},"description":"Almost loss of activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30150682"}],"alternativeSequence":{"originalSequence":"R","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":188,"modifier":"EXACT"},"end":{"value":188,"modifier":"EXACT"}},"description":"No change in activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30150682"}],"alternativeSequence":{"originalSequence":"I","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":206,"modifier":"EXACT"},"end":{"value":206,"modifier":"EXACT"}},"description":"Decrease in activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30150682"}],"alternativeSequence":{"originalSequence":"S","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":208,"modifier":"EXACT"},"end":{"value":208,"modifier":"EXACT"}},"description":"Strong decrease in activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30150682"}],"alternativeSequence":{"originalSequence":"T","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":211,"modifier":"EXACT"},"end":{"value":211,"modifier":"EXACT"}},"description":"Almost loss of activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30150682"}],"alternativeSequence":{"originalSequence":"R","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":253,"modifier":"EXACT"},"end":{"value":253,"modifier":"EXACT"}},"description":"Cannot form a dimer and has no activity; when associated with E-256.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30150682"}],"alternativeSequence":{"originalSequence":"Y","alternativeSequences":["E"]}},{"type":"Mutagenesis","location":{"start":{"value":256,"modifier":"EXACT"},"end":{"value":256,"modifier":"EXACT"}},"description":"Cannot form a dimer and has no activity; when associated with E-253.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30150682"}],"alternativeSequence":{"originalSequence":"Y","alternativeSequences":["E"]}},{"type":"Mutagenesis","location":{"start":{"value":279,"modifier":"EXACT"},"end":{"value":279,"modifier":"EXACT"}},"description":"Strong decrease in activity. Decreases tRNA binding.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30150682"}],"alternativeSequence":{"originalSequence":"R","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":298,"modifier":"EXACT"},"end":{"value":298,"modifier":"EXACT"}},"description":"Strong decrease in activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30150682"}],"alternativeSequence":{"originalSequence":"K","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":300,"modifier":"EXACT"},"end":{"value":300,"modifier":"EXACT"}},"description":"Strong decrease in activity. Decreases tRNA binding.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30150682"}],"alternativeSequence":{"originalSequence":"K","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":301,"modifier":"EXACT"},"end":{"value":301,"modifier":"EXACT"}},"description":"Almost loss of activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30150682"}],"alternativeSequence":{"originalSequence":"R","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":306,"modifier":"EXACT"},"end":{"value":306,"modifier":"EXACT"}},"description":"Almost loss of activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30150682"}],"alternativeSequence":{"originalSequence":"R","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":309,"modifier":"EXACT"},"end":{"value":309,"modifier":"EXACT"}},"description":"Almost loss of activity. Decreases tRNA binding.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30150682"}],"alternativeSequence":{"originalSequence":"R","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":352,"modifier":"EXACT"},"end":{"value":352,"modifier":"EXACT"}},"description":"Strong decrease in activity. Decreases tRNA binding.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30150682"}],"alternativeSequence":{"originalSequence":"K","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":353,"modifier":"EXACT"},"end":{"value":353,"modifier":"EXACT"}},"description":"No change in activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30150682"}],"alternativeSequence":{"originalSequence":"K","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":362,"modifier":"EXACT"},"end":{"value":362,"modifier":"EXACT"}},"description":"Strong decrease in activity. Decreases tRNA binding.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30150682"}],"alternativeSequence":{"originalSequence":"K","alternativeSequences":["A"]}},{"type":"Beta strand","location":{"start":{"value":3,"modifier":"EXACT"},"end":{"value":8,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5Y0Q"}]},{"type":"Helix","location":{"start":{"value":15,"modifier":"EXACT"},"end":{"value":28,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5Y0Q"}]},{"type":"Beta strand","location":{"start":{"value":31,"modifier":"EXACT"},"end":{"value":38,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5Y0Q"}]},{"type":"Helix","location":{"start":{"value":52,"modifier":"EXACT"},"end":{"value":60,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5Y0Q"}]},{"type":"Turn","location":{"start":{"value":61,"modifier":"EXACT"},"end":{"value":63,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5Y0Q"}]},{"type":"Beta strand","location":{"start":{"value":65,"modifier":"EXACT"},"end":{"value":70,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5Y0Q"}]},{"type":"Helix","location":{"start":{"value":72,"modifier":"EXACT"},"end":{"value":75,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5Y0Q"}]},{"type":"Helix","location":{"start":{"value":79,"modifier":"EXACT"},"end":{"value":92,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5Y0Q"}]},{"type":"Beta strand","location":{"start":{"value":96,"modifier":"EXACT"},"end":{"value":103,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5Y0Q"}]},{"type":"Helix","location":{"start":{"value":108,"modifier":"EXACT"},"end":{"value":133,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5Y0Q"}]},{"type":"Helix","location":{"start":{"value":138,"modifier":"EXACT"},"end":{"value":150,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5Y0Q"}]},{"type":"Beta strand","location":{"start":{"value":158,"modifier":"EXACT"},"end":{"value":160,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5Y0Q"}]},{"type":"Helix","location":{"start":{"value":161,"modifier":"EXACT"},"end":{"value":176,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5Y0Q"}]},{"type":"Beta strand","location":{"start":{"value":181,"modifier":"EXACT"},"end":{"value":186,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5Y0Q"}]},{"type":"Beta strand","location":{"start":{"value":192,"modifier":"EXACT"},"end":{"value":195,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5Y0O"}]},{"type":"Helix","location":{"start":{"value":207,"modifier":"EXACT"},"end":{"value":215,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5Y0Q"}]},{"type":"Beta strand","location":{"start":{"value":220,"modifier":"EXACT"},"end":{"value":222,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5Y0Q"}]},{"type":"Helix","location":{"start":{"value":223,"modifier":"EXACT"},"end":{"value":225,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5Y0Q"}]},{"type":"Helix","location":{"start":{"value":228,"modifier":"EXACT"},"end":{"value":241,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5Y0Q"}]},{"type":"Helix","location":{"start":{"value":247,"modifier":"EXACT"},"end":{"value":250,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5Y0Q"}]},{"type":"Helix","location":{"start":{"value":251,"modifier":"EXACT"},"end":{"value":257,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5Y0Q"}]},{"type":"Helix","location":{"start":{"value":263,"modifier":"EXACT"},"end":{"value":267,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5Y0Q"}]},{"type":"Helix","location":{"start":{"value":276,"modifier":"EXACT"},"end":{"value":286,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5Y0Q"}]},{"type":"Helix","location":{"start":{"value":290,"modifier":"EXACT"},"end":{"value":297,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5Y0Q"}]},{"type":"Beta strand","location":{"start":{"value":300,"modifier":"EXACT"},"end":{"value":302,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5Y0N"}]},{"type":"Helix","location":{"start":{"value":304,"modifier":"EXACT"},"end":{"value":316,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5Y0Q"}]},{"type":"Helix","location":{"start":{"value":320,"modifier":"EXACT"},"end":{"value":328,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5Y0Q"}]},{"type":"Beta strand","location":{"start":{"value":329,"modifier":"EXACT"},"end":{"value":331,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5Y0Q"}]},{"type":"Beta strand","location":{"start":{"value":335,"modifier":"EXACT"},"end":{"value":340,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5Y0Q"}]},{"type":"Helix","location":{"start":{"value":342,"modifier":"EXACT"},"end":{"value":351,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5Y0Q"}]},{"type":"Turn","location":{"start":{"value":352,"modifier":"EXACT"},"end":{"value":354,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5Y0Q"}]},{"type":"Beta strand","location":{"start":{"value":359,"modifier":"EXACT"},"end":{"value":362,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5Y0Q"}]},{"type":"Helix","location":{"start":{"value":363,"modifier":"EXACT"},"end":{"value":365,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5Y0Q"}]},{"type":"Helix","location":{"start":{"value":369,"modifier":"EXACT"},"end":{"value":381,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5Y0Q"}]},{"type":"Turn","location":{"start":{"value":382,"modifier":"EXACT"},"end":{"value":384,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5Y0Q"}]},{"type":"Helix","location":{"start":{"value":389,"modifier":"EXACT"},"end":{"value":399,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5Y0Q"}]},{"type":"Helix","location":{"start":{"value":400,"modifier":"EXACT"},"end":{"value":402,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5Y0Q"}]},{"type":"Beta strand","location":{"start":{"value":403,"modifier":"EXACT"},"end":{"value":406,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5Y0Q"}]},{"type":"Turn","location":{"start":{"value":407,"modifier":"EXACT"},"end":{"value":410,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5Y0Q"}]}],"keywords":[{"id":"KW-0002","category":"Technical term","name":"3D-structure"},{"id":"KW-0067","category":"Ligand","name":"ATP-binding"},{"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"id":"KW-0436","category":"Molecular function","name":"Ligase"},{"id":"KW-0547","category":"Ligand","name":"Nucleotide-binding"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0694","category":"Molecular function","name":"RNA-binding"},{"id":"KW-0819","category":"Biological process","name":"tRNA processing"},{"id":"KW-0820","category":"Molecular function","name":"tRNA-binding"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-EI7QA3AUL5QQK","citationType":"submission","authors":["Bertero M.","Presecan E.","Glaser P.","Richou A.","Danchin A."],"title":"Bacillus subtilis chromosomal region downstream nprE.","publicationDate":"AUG-1997","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"],"referenceComments":[{"value":"168","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"9384377","citationType":"journal article","authors":["Kunst F.","Ogasawara N.","Moszer I.","Albertini A.M.","Alloni G.","Azevedo V.","Bertero M.G.","Bessieres P.","Bolotin A.","Borchert S.","Borriss R.","Boursier L.","Brans A.","Braun M.","Brignell S.C.","Bron S.","Brouillet S.","Bruschi C.V.","Caldwell B.","Capuano V.","Carter N.M.","Choi S.-K.","Codani J.-J.","Connerton I.F.","Cummings N.J.","Daniel R.A.","Denizot F.","Devine K.M.","Duesterhoeft A.","Ehrlich S.D.","Emmerson P.T.","Entian K.-D.","Errington J.","Fabret C.","Ferrari E.","Foulger D.","Fritz C.","Fujita M.","Fujita Y.","Fuma S.","Galizzi A.","Galleron N.","Ghim S.-Y.","Glaser P.","Goffeau A.","Golightly E.J.","Grandi G.","Guiseppi G.","Guy B.J.","Haga K.","Haiech J.","Harwood C.R.","Henaut A.","Hilbert H.","Holsappel S.","Hosono S.","Hullo M.-F.","Itaya M.","Jones L.-M.","Joris B.","Karamata D.","Kasahara Y.","Klaerr-Blanchard M.","Klein C.","Kobayashi Y.","Koetter P.","Koningstein G.","Krogh S.","Kumano M.","Kurita K.","Lapidus A.","Lardinois S.","Lauber J.","Lazarevic V.","Lee S.-M.","Levine A.","Liu H.","Masuda S.","Mauel C.","Medigue C.","Medina N.","Mellado R.P.","Mizuno M.","Moestl D.","Nakai S.","Noback M.","Noone D.","O'Reilly M.","Ogawa K.","Ogiwara A.","Oudega B.","Park S.-H.","Parro V.","Pohl T.M.","Portetelle D.","Porwollik S.","Prescott A.M.","Presecan E.","Pujic P.","Purnelle B.","Rapoport G.","Rey M.","Reynolds S.","Rieger M.","Rivolta C.","Rocha E.","Roche B.","Rose M.","Sadaie Y.","Sato T.","Scanlan E.","Schleich S.","Schroeter R.","Scoffone F.","Sekiguchi J.","Sekowska A.","Seror S.J.","Serror P.","Shin B.-S.","Soldo B.","Sorokin A.","Tacconi E.","Takagi T.","Takahashi H.","Takemaru K.","Takeuchi M.","Tamakoshi A.","Tanaka T.","Terpstra P.","Tognoni A.","Tosato V.","Uchiyama S.","Vandenbol M.","Vannier F.","Vassarotti A.","Viari A.","Wambutt R.","Wedler E.","Wedler H.","Weitzenegger T.","Winters P.","Wipat A.","Yamamoto H.","Yamane K.","Yasumoto K.","Yata K.","Yoshida K.","Yoshikawa H.-F.","Zumstein E.","Yoshikawa H.","Danchin A."],"citationCrossReferences":[{"database":"PubMed","id":"9384377"},{"database":"DOI","id":"10.1038/36786"}],"title":"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis.","publicationDate":"1997","journal":"Nature","firstPage":"249","lastPage":"256","volume":"390"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"168","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"30150682","citationType":"journal article","authors":["Taniguchi T.","Miyauchi K.","Sakaguchi Y.","Yamashita S.","Soma A.","Tomita K.","Suzuki T."],"citationCrossReferences":[{"database":"PubMed","id":"30150682"},{"database":"DOI","id":"10.1038/s41589-018-0119-z"}],"title":"Acetate-dependent tRNA acetylation required for decoding fidelity in protein synthesis.","publicationDate":"2018","journal":"Nat. Chem. Biol.","firstPage":"1010","lastPage":"1020","volume":"14"},"referencePositions":["X-RAY CRYSTALLOGRAPHY (2.10 ANGSTROMS) IN COMPLEXES WITH ATP AND ATP ANALOGS","FUNCTION","CATALYTIC ACTIVITY","SUBUNIT","DISRUPTION PHENOTYPE","MUTAGENESIS OF GLU-9; HIS-14; HIS-17; HIS-20; ARG-44; LYS-129; LYS-133; LYS-134; ASN-162; ARG-187; ILE-188; SER-206; THR-208; ARG-211; TYR-253; TYR-256; ARG-279; LYS-298; LYS-300; ARG-301; ARG-306; ARG-309; LYS-352; LYS-353 AND LYS-362"],"referenceComments":[{"value":"168","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0007744","source":"PDB","id":"5Y0N"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"5Y0O"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"5Y0P"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"5Y0Q"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"Z98682","properties":[{"key":"ProteinId","value":"CAB11359.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AL009126","properties":[{"key":"ProteinId","value":"CAB13379.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"B69875","properties":[{"key":"EntryName","value":"B69875"}]},{"database":"RefSeq","id":"NP_389389.1","properties":[{"key":"NucleotideSequenceId","value":"NC_000964.3"}]},{"database":"RefSeq","id":"WP_003244707.1","properties":[{"key":"NucleotideSequenceId","value":"NC_000964.3"}]},{"database":"PDB","id":"5Y0N","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.30 A"},{"key":"Chains","value":"A/B=1-415"}]},{"database":"PDB","id":"5Y0O","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.30 A"},{"key":"Chains","value":"A/B=1-415"}]},{"database":"PDB","id":"5Y0P","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.30 A"},{"key":"Chains","value":"A/B=1-415"}]},{"database":"PDB","id":"5Y0Q","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.10 A"},{"key":"Chains","value":"A/B=1-415"}]},{"database":"PDBsum","id":"5Y0N","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"5Y0O","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"5Y0P","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"5Y0Q","properties":[{"key":"Description","value":"-"}]},{"database":"AlphaFoldDB","id":"O34513","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"O34513","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"O34513","properties":[{"key":"Number of interactors","value":"70"}]},{"database":"STRING","id":"224308.BSU15060","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"224308-BSU15060","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblBacteria","id":"CAB13379","properties":[{"key":"ProteinId","value":"CAB13379"},{"key":"GeneId","value":"BSU_15060"}]},{"database":"GeneID","id":"936548","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"bsu:BSU15060","properties":[{"key":"Description","value":"-"}]},{"database":"PATRIC","id":"fig|224308.179.peg.1641","properties":[{"key":"GeneDesignation","value":"-"}]},{"database":"eggNOG","id":"COG1323","properties":[{"key":"ToxonomicScope","value":"Bacteria"}]},{"database":"InParanoid","id":"O34513","properties":[{"key":"Description","value":"-"}]},{"database":"OrthoDB","id":"9769796at2","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"O34513","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"BSUB:BSU15060-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"BRENDA","id":"6.3.1.22","properties":[{"key":"OrganismId","value":"658"}]},{"database":"Proteomes","id":"UP000001570","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0016879","properties":[{"key":"GoTerm","value":"F:ligase activity, forming carbon-nitrogen bonds"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0006400","properties":[{"key":"GoTerm","value":"P:tRNA modification"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"Gene3D","id":"3.40.50.620","properties":[{"key":"EntryName","value":"HUPs"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_01539","properties":[{"key":"EntryName","value":"TmcAL"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR014729","properties":[{"key":"EntryName","value":"Rossmann-like_a/b/a_fold"}]},{"database":"InterPro","id":"IPR008513","properties":[{"key":"EntryName","value":"tRNA(Met)_cyd_acetate_ligase"}]},{"database":"NCBIfam","id":"NF010191","properties":[{"key":"EntryName","value":"PRK13670.1"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR37825","properties":[{"key":"EntryName","value":"TRNA(MET) CYTIDINE ACETATE LIGASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR37825:SF1","properties":[{"key":"EntryName","value":"TRNA(MET) CYTIDINE ACETATE LIGASE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF05636","properties":[{"key":"EntryName","value":"HIGH_NTase1"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF52374","properties":[{"key":"EntryName","value":"Nucleotidylyl transferase"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MKAVGLVVEYNPFHNGHLYHAQTAKLQTGCDTAVAVMSGHFLQRGEPAVVSKWARTKMALQSGVDLVIELPYLYAVQKADIFARGSVSILNELECEALFFGSENGDIKPFLETAQLIDEHKHILNDRIKEELKKGASYPAAAAIAFSSILHTESALDLSKPNNILGYQYVTSILTGGYPMKPYTTARISSDYHDADLPEGENHIASATSIRKAMIGQNLEACLRFLPAASARELAAYRKSFGLWHTPESYFSYLKYSLSTVTARELQQVYEVEEGLEHRIIRSIRKSSSYQEFMELLKTKRYTWTRLQRMNTHILTRTKKQDMQKLLDNDKAPYIRLLGMTKKGQAYLSEKKKALSVPLVSKLSSFSHPALDLDVKASRIYSLPIEEPLRTEFDLQEYGHAPIRYDEDEQHFLNV","length":415,"molWeight":46989,"crc64":"4776188611CEC2C6","md5":"F747BA11F70E701DC5EFED8EB3BA8FC0"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":3,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"DISRUPTION PHENOTYPE":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Binding site":4,"Mutagenesis":25,"Beta strand":13,"Helix":22,"Turn":4},"uniParcId":"UPI000006036F"}}