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Is the major exporter of plipastatin, an antimicrobial lipopeptide produced by B.subtilis that exhibits remarkable antimicrobial activity against a diverse range of pathogenic bacteria and fungi (PubMed:38891014, PubMed:39719240). Is specific for plipastatin and does not transport surfactin and iturin (PubMed:38891014, PubMed:39719240). In addition, overexpression enhances the resistance to various antibiotics, including penicillin, ampicillin, tetracycline and vancomycin (PubMed:39719240). It confers plipastatin self-resistance (PubMed:39719240). It can also export the toxic chemical ethidium bromide (EtBr) (PubMed:39719240). The efflux activity is Na(+)-dependent and is not dependent on H(+) concentration (PubMed:39719240)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"39719240"}],"value":"Specific concentrations of Cu(2+) significantly enhance the efflux activity, promoting the transport of plipastatin out of the cell (PubMed:39719240). The ethidium bromide (EtBr) efflux activity is inhibited by carbonyl cyanide chlorophenylhydrazone (CCCP) and enhanced by Na(+) (PubMed:39719240)"}],"commentType":"ACTIVITY REGULATION"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Cell membrane","id":"SL-0039"},"topology":{"evidences":[{"evidenceCode":"ECO:0000255"}],"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"39719240"}],"value":"The expression is induced by plipastatin and in response to various antibiotics such as tetracycline, vancomycin, ampicillin, penicillin and acriflavine (PubMed:39719240). Also induced by ethidium bromide (PubMed:39719240)"}],"commentType":"INDUCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"38891014"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"39719240"}],"value":"Knockout of the gene decreases plipastatin production but has no significant effect on the production of surfactin and iturin (PubMed:38891014). The deletion mutant shows higher sensitivity to plipastatin and reduced resistance to vancomycin, ampicillin, tetracycline, penicillin and acriflavine (PubMed:39719240)"}],"commentType":"DISRUPTION PHENOTYPE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":463,"modifier":"EXACT"}},"description":"Multidrug resistance transport protein 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