{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"O34206","uniProtkbId":"KINB_PSEAI","entryAudit":{"firstPublicDate":"2004-04-26","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"1998-01-01","entryVersion":140,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Pseudomonas aeruginosa","taxonId":287,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Pseudomonadales","Pseudomonadaceae","Pseudomonas"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Alginate biosynthesis sensor protein KinB"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9218420"}],"value":"2.7.13.3"}]}},"genes":[{"geneName":{"value":"kinB"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9218420"}],"value":"Member of the two-component regulatory system AlgB/KinB involved in regulation of alginate biosynthesis genes. KinB functions as a membrane-associated protein kinase that phosphorylates AlgB, probably in response to environmental signals"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.","ecNumber":"2.7.13.3","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9218420"}]}},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9218420"}],"value":"Cell inner membrane","id":"SL-0037"},"topology":{"evidences":[{"evidenceCode":"ECO:0000255"}],"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9218420"}],"value":"Autophosphorylated"}],"commentType":"PTM"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":595,"modifier":"EXACT"}},"description":"Alginate biosynthesis sensor protein KinB","featureId":"PRO_0000074778"},{"type":"Topological domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":12,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"9218420"}]},{"type":"Transmembrane","location":{"start":{"value":13,"modifier":"EXACT"},"end":{"value":33,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":34,"modifier":"EXACT"},"end":{"value":167,"modifier":"EXACT"}},"description":"Periplasmic","evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"9218420"}]},{"type":"Transmembrane","location":{"start":{"value":168,"modifier":"EXACT"},"end":{"value":188,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":189,"modifier":"EXACT"},"end":{"value":595,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"9218420"}]},{"type":"Domain","location":{"start":{"value":195,"modifier":"EXACT"},"end":{"value":247,"modifier":"EXACT"}},"description":"HAMP","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00102"}]},{"type":"Domain","location":{"start":{"value":258,"modifier":"EXACT"},"end":{"value":323,"modifier":"EXACT"}},"description":"PAS","evidences":[{"evidenceCode":"ECO:0000305"}]},{"type":"Domain","location":{"start":{"value":327,"modifier":"EXACT"},"end":{"value":369,"modifier":"EXACT"}},"description":"PAC","evidences":[{"evidenceCode":"ECO:0000305"}]},{"type":"Domain","location":{"start":{"value":382,"modifier":"EXACT"},"end":{"value":595,"modifier":"EXACT"}},"description":"Histidine kinase","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00107"}]},{"type":"Modified residue","location":{"start":{"value":385,"modifier":"EXACT"},"end":{"value":385,"modifier":"EXACT"}},"description":"Phosphohistidine; by autocatalysis","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00107"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9218420"}]},{"type":"Mutagenesis","location":{"start":{"value":385,"modifier":"EXACT"},"end":{"value":385,"modifier":"EXACT"}},"description":"Loss of autophosphorylation.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9218420"}],"alternativeSequence":{"originalSequence":"H","alternativeSequences":["K","Q"]}},{"type":"Mutagenesis","location":{"start":{"value":504,"modifier":"EXACT"},"end":{"value":504,"modifier":"EXACT"}},"description":"Loss of autophosphorylation.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9218420"}],"alternativeSequence":{"originalSequence":"N","alternativeSequences":["Q"]}},{"type":"Mutagenesis","location":{"start":{"value":532,"modifier":"EXACT"},"end":{"value":532,"modifier":"EXACT"}},"description":"Loss of autophosphorylation.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9218420"}],"alternativeSequence":{"originalSequence":"D","alternativeSequences":["E"]}},{"type":"Mutagenesis","location":{"start":{"value":532,"modifier":"EXACT"},"end":{"value":532,"modifier":"EXACT"}},"description":"Residual autophosphorylation.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9218420"}],"alternativeSequence":{"originalSequence":"D","alternativeSequences":["N"]}},{"type":"Mutagenesis","location":{"start":{"value":560,"modifier":"EXACT"},"end":{"value":560,"modifier":"EXACT"}},"description":"Loss of autophosphorylation.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9218420"}],"alternativeSequence":{"originalSequence":"G","alternativeSequences":["A"]}},{"type":"Helix","location":{"start":{"value":42,"modifier":"EXACT"},"end":{"value":70,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3KKB"}]},{"type":"Beta strand","location":{"start":{"value":72,"modifier":"EXACT"},"end":{"value":74,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3KKB"}]},{"type":"Helix","location":{"start":{"value":77,"modifier":"EXACT"},"end":{"value":97,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3KKB"}]},{"type":"Helix","location":{"start":{"value":101,"modifier":"EXACT"},"end":{"value":118,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3KKB"}]},{"type":"Helix","location":{"start":{"value":121,"modifier":"EXACT"},"end":{"value":126,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3KKB"}]},{"type":"Beta strand","location":{"start":{"value":131,"modifier":"EXACT"},"end":{"value":133,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3KKB"}]},{"type":"Helix","location":{"start":{"value":134,"modifier":"EXACT"},"end":{"value":162,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3KKB"}]}],"keywords":[{"id":"KW-0002","category":"Technical term","name":"3D-structure"},{"id":"KW-0016","category":"Biological process","name":"Alginate biosynthesis"},{"id":"KW-0067","category":"Ligand","name":"ATP-binding"},{"id":"KW-0997","category":"Cellular component","name":"Cell inner membrane"},{"id":"KW-1003","category":"Cellular component","name":"Cell membrane"},{"id":"KW-0418","category":"Molecular function","name":"Kinase"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-0547","category":"Ligand","name":"Nucleotide-binding"},{"id":"KW-0597","category":"PTM","name":"Phosphoprotein"},{"id":"KW-0716","category":"Biological process","name":"Sensory transduction"},{"id":"KW-0808","category":"Molecular function","name":"Transferase"},{"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"id":"KW-1133","category":"Domain","name":"Transmembrane helix"}],"references":[{"referenceNumber":1,"citation":{"id":"9218420","citationType":"journal article","authors":["Ma S.","Wozniak D.J.","Ohman D.E."],"citationCrossReferences":[{"database":"PubMed","id":"9218420"},{"database":"DOI","id":"10.1074/jbc.272.29.17952"}],"title":"Identification of the histidine protein kinase kinB in Pseudomonas aeruginosa and its phosphorylation of the alginate regulator algB.","publicationDate":"1997","journal":"J. Biol. Chem.","firstPage":"17952","lastPage":"17960","volume":"272"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]","FUNCTION","CATALYTIC ACTIVITY","SUBCELLULAR LOCATION","TOPOLOGY","PHOSPHORYLATION AT HIS-385","MUTAGENESIS OF HIS-385; ASN-504; ASP-532 AND GLY-560"],"referenceComments":[{"value":"FRD444","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"U97063","properties":[{"key":"ProteinId","value":"AAB68790.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PDB","id":"3KKB","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.88 A"},{"key":"Chains","value":"A/B=42-168"}]},{"database":"PDBsum","id":"3KKB","properties":[{"key":"Description","value":"-"}]},{"database":"AlphaFoldDB","id":"O34206","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"O34206","properties":[{"key":"Description","value":"-"}]},{"database":"iPTMnet","id":"O34206","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"COG5002","properties":[{"key":"ToxonomicScope","value":"Bacteria"}]},{"database":"BRENDA","id":"2.7.13.3","properties":[{"key":"OrganismId","value":"5087"}]},{"database":"EvolutionaryTrace","id":"O34206","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005886","properties":[{"key":"GoTerm","value":"C:plasma membrane"},{"key":"GoEvidenceType","value":"EXP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9218420"}]},{"database":"GO","id":"GO:0000155","properties":[{"key":"GoTerm","value":"F:phosphorelay sensor kinase activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0004673","properties":[{"key":"GoTerm","value":"F:protein histidine kinase activity"},{"key":"GoEvidenceType","value":"EXP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9218420"}]},{"database":"CDD","id":"cd06225","properties":[{"key":"EntryName","value":"HAMP"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd00075","properties":[{"key":"EntryName","value":"HATPase"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd00082","properties":[{"key":"EntryName","value":"HisKA"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd19409","properties":[{"key":"EntryName","value":"KinB_sensor"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd00130","properties":[{"key":"EntryName","value":"PAS"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.30.565.10:FF:000006","properties":[{"key":"EntryName","value":"Sensor histidine kinase WalK"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.287.130","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.8.500","properties":[{"key":"EntryName","value":"HAMP domain in histidine kinase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.20.120.880","properties":[{"key":"EntryName","value":"Histidine kinase (KinB), sensor domain"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.565.10","properties":[{"key":"EntryName","value":"Histidine kinase-like ATPase, C-terminal domain"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.450.20","properties":[{"key":"EntryName","value":"PAS domain"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR050980","properties":[{"key":"EntryName","value":"2C_sensor_his_kinase"}]},{"database":"InterPro","id":"IPR003660","properties":[{"key":"EntryName","value":"HAMP_dom"}]},{"database":"InterPro","id":"IPR036890","properties":[{"key":"EntryName","value":"HATPase_C_sf"}]},{"database":"InterPro","id":"IPR003594","properties":[{"key":"EntryName","value":"HATPase_dom"}]},{"database":"InterPro","id":"IPR005467","properties":[{"key":"EntryName","value":"His_kinase_dom"}]},{"database":"InterPro","id":"IPR003661","properties":[{"key":"EntryName","value":"HisK_dim/P_dom"}]},{"database":"InterPro","id":"IPR036097","properties":[{"key":"EntryName","value":"HisK_dim/P_sf"}]},{"database":"InterPro","id":"IPR038320","properties":[{"key":"EntryName","value":"KinB_N_sf"}]},{"database":"InterPro","id":"IPR031909","properties":[{"key":"EntryName","value":"KinB_sensor_dom"}]},{"database":"InterPro","id":"IPR000014","properties":[{"key":"EntryName","value":"PAS"}]},{"database":"InterPro","id":"IPR035965","properties":[{"key":"EntryName","value":"PAS-like_dom_sf"}]},{"database":"InterPro","id":"IPR013656","properties":[{"key":"EntryName","value":"PAS_4"}]},{"database":"InterPro","id":"IPR004358","properties":[{"key":"EntryName","value":"Sig_transdc_His_kin-like_C"}]},{"database":"NCBIfam","id":"TIGR00229","properties":[{"key":"EntryName","value":"sensory_box"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR44936","properties":[{"key":"EntryName","value":"SENSOR PROTEIN CREC"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR44936:SF10","properties":[{"key":"EntryName","value":"SENSOR PROTEIN RSTB"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00672","properties":[{"key":"EntryName","value":"HAMP"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF02518","properties":[{"key":"EntryName","value":"HATPase_c"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00512","properties":[{"key":"EntryName","value":"HisKA"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF16767","properties":[{"key":"EntryName","value":"KinB_sensor"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF08448","properties":[{"key":"EntryName","value":"PAS_4"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR00344","properties":[{"key":"EntryName","value":"BCTRLSENSOR"}]},{"database":"SMART","id":"SM00304","properties":[{"key":"EntryName","value":"HAMP"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00387","properties":[{"key":"EntryName","value":"HATPase_c"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00388","properties":[{"key":"EntryName","value":"HisKA"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00091","properties":[{"key":"EntryName","value":"PAS"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF55874","properties":[{"key":"EntryName","value":"ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF158472","properties":[{"key":"EntryName","value":"HAMP domain-like"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF47384","properties":[{"key":"EntryName","value":"Homodimeric domain of signal transducing histidine kinase"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF55785","properties":[{"key":"EntryName","value":"PYP-like sensor domain (PAS domain)"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS50885","properties":[{"key":"EntryName","value":"HAMP"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS50109","properties":[{"key":"EntryName","value":"HIS_KIN"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MSMPLPMKLRTRLFLSISALITVSLFGLLLGLFSVMQLGRAQEQRMSHHYATIEVSQQLRQLLGDQLVILLRETPDGQALERSQNDFRRVLEQGRANTVDSAEQAALDGVRDAYLQLQAHTPALLEAPMVDNDGFSEAFNGLRLRLQDLQQLALAGISDAETSARHRAYLVAGLLGLVGVAILLIGFVTAHSIARRFGAPIETLARAADRIGEGDFDVTLPMTNVAEVGQLTRRFGLMAEALRQYRKTSVEEVLSGERRLQAVLDSIDDGLVIFDNQGRIEHANPVAIRQLFVSNDPHGKRIDEILSDVDVQEAVEKALLGEVQDEAMPDLVVDVAGESRLLAWSLYPVTHPGGHSVGAVLVVRDVTEQRAFERVRSEFVLRASHELRTPVTGMQMAFSLLRERLDFPAESREADLIQTVDEEMSRLVLLINDLLNFSRYQTGMQKLELASCDLVDLLTQAQQRFIPKGEARRVSLQLELGDELPRLQLDRLQIERVIDNLLENALRHSSEGGQIHLQARRQGDRVLIAVEDNGEGIPFSQQGRIFEPFVQVGRKKGGAGLGLELCKEIIQLHGGRIAVRSQPGQGARFYMLLPV","length":595,"molWeight":66072,"crc64":"BA12FC7E3120F048","md5":"8360ED5E60385159B4D311B5DFB0917D"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"SUBCELLULAR LOCATION":1,"PTM":1},"countByFeatureType":{"Chain":1,"Topological domain":3,"Transmembrane":2,"Domain":4,"Modified residue":1,"Mutagenesis":5,"Helix":5,"Beta strand":2},"uniParcId":"UPI00000BEEE0"}}