{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"O32031","uniProtkbId":"RAGB_BACSU","entryAudit":{"firstPublicDate":"2009-12-15","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"1998-01-01","entryVersion":101,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Bacillus subtilis (strain 168)","taxonId":224308,"lineage":["Bacteria","Bacillati","Bacillota","Bacilli","Caryophanales","Bacillaceae","Bacillus"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Regulatory protein RagB"}},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"40817173"}],"value":"RodA GT booster"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"40817173"}],"value":"RodA booster"}]}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"40817173"}],"value":"ragB"},"synonyms":[{"value":"yrrS"}],"orderedLocusNames":[{"value":"BSU27300"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"40817173"}],"value":"Regulator that modulates the activity of the peptidoglycan glycosyltransferase RodA (PubMed:40817173). RagB stimulates RodA activity via a protein-protein interaction (PubMed:40817173). It does not affect the expression, protein levels, localization or dynamics of RodA (PubMed:40817173). Is necessary for cell growth and morphology of B.subtilis in the absence of class A penicillin-binding proteins (aPBPs) and in particular of the major aPBP, PBP1 (PubMed:40817173). For example, it is important for the survival of bacteria in the presence of the antibiotic moenomycin, which specifically inhibits the glycosyltransferase activity of aPBP (PubMed:40817173)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"40817173"}],"value":"Interacts with RodA"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"40817173"}],"value":"Forms randomly diffusing foci in the membrane (PubMed:40817173). Is enriched at the septum during the exponential phase and in the sidewalls during the stationary phase (PubMed:40817173)"}]},"subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"40817173"}],"value":"Cell membrane","id":"SL-0039"},"topology":{"evidences":[{"evidenceCode":"ECO:0000255"}],"value":"Single-pass membrane protein","id":"SL-9904"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"40817173"}],"value":"Deletion of the gene does not affect growth and morphology in a wild-type background, but it causes strong growth and morphological defects in the absence of PBP1 (PubMed:40817173). The deletion mutant is slightly more sensitive to moenomycin than the wild-type strain (PubMed:40817173). The absence of RagB is compensated by the overproduction of RodA (PubMed:40817173)"}],"commentType":"DISRUPTION PHENOTYPE"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":233,"modifier":"EXACT"}},"description":"Regulatory protein RagB","featureId":"PRO_0000390391"},{"type":"Transmembrane","location":{"start":{"value":21,"modifier":"EXACT"},"end":{"value":41,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Region","location":{"start":{"value":44,"modifier":"EXACT"},"end":{"value":163,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":48,"modifier":"EXACT"},"end":{"value":57,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":66,"modifier":"EXACT"},"end":{"value":108,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":135,"modifier":"EXACT"},"end":{"value":144,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]}],"keywords":[{"id":"KW-1003","category":"Cellular component","name":"Cell membrane"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"id":"KW-1133","category":"Domain","name":"Transmembrane helix"}],"references":[{"referenceNumber":1,"citation":{"id":"9384377","citationType":"journal article","authors":["Kunst F.","Ogasawara N.","Moszer I.","Albertini A.M.","Alloni G.","Azevedo V.","Bertero M.G.","Bessieres P.","Bolotin A.","Borchert S.","Borriss R.","Boursier L.","Brans A.","Braun M.","Brignell S.C.","Bron S.","Brouillet S.","Bruschi C.V.","Caldwell B.","Capuano V.","Carter N.M.","Choi S.-K.","Codani J.-J.","Connerton I.F.","Cummings N.J.","Daniel R.A.","Denizot F.","Devine K.M.","Duesterhoeft A.","Ehrlich S.D.","Emmerson P.T.","Entian K.-D.","Errington J.","Fabret C.","Ferrari E.","Foulger D.","Fritz C.","Fujita M.","Fujita Y.","Fuma S.","Galizzi A.","Galleron N.","Ghim S.-Y.","Glaser P.","Goffeau A.","Golightly E.J.","Grandi G.","Guiseppi G.","Guy B.J.","Haga K.","Haiech J.","Harwood C.R.","Henaut A.","Hilbert H.","Holsappel S.","Hosono S.","Hullo M.-F.","Itaya M.","Jones L.-M.","Joris B.","Karamata D.","Kasahara Y.","Klaerr-Blanchard M.","Klein C.","Kobayashi Y.","Koetter P.","Koningstein G.","Krogh S.","Kumano M.","Kurita K.","Lapidus A.","Lardinois S.","Lauber J.","Lazarevic V.","Lee S.-M.","Levine A.","Liu H.","Masuda S.","Mauel C.","Medigue C.","Medina N.","Mellado R.P.","Mizuno M.","Moestl D.","Nakai S.","Noback M.","Noone D.","O'Reilly M.","Ogawa K.","Ogiwara A.","Oudega B.","Park S.-H.","Parro V.","Pohl T.M.","Portetelle D.","Porwollik S.","Prescott A.M.","Presecan E.","Pujic P.","Purnelle B.","Rapoport G.","Rey M.","Reynolds S.","Rieger M.","Rivolta C.","Rocha E.","Roche B.","Rose M.","Sadaie Y.","Sato T.","Scanlan E.","Schleich S.","Schroeter R.","Scoffone F.","Sekiguchi J.","Sekowska A.","Seror S.J.","Serror P.","Shin B.-S.","Soldo B.","Sorokin A.","Tacconi E.","Takagi T.","Takahashi H.","Takemaru K.","Takeuchi M.","Tamakoshi A.","Tanaka T.","Terpstra P.","Tognoni A.","Tosato V.","Uchiyama S.","Vandenbol M.","Vannier F.","Vassarotti A.","Viari A.","Wambutt R.","Wedler E.","Wedler H.","Weitzenegger T.","Winters P.","Wipat A.","Yamamoto H.","Yamane K.","Yasumoto K.","Yata K.","Yoshida K.","Yoshikawa H.-F.","Zumstein E.","Yoshikawa H.","Danchin A."],"citationCrossReferences":[{"database":"PubMed","id":"9384377"},{"database":"DOI","id":"10.1038/36786"}],"title":"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis.","publicationDate":"1997","journal":"Nature","firstPage":"249","lastPage":"256","volume":"390"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"168","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"40817173","citationType":"journal article","authors":["Pompeo F.","Foulquier E.","Chastanet A.","Espinosa L.","Billaudeau C.","Rodrigues A.","Cornilleau C.","Carballido-Lopez R.","Galinier A."],"citationCrossReferences":[{"database":"PubMed","id":"40817173"},{"database":"DOI","id":"10.1038/s44319-025-00547-w"}],"title":"RagB stimulates the activity of the peptidoglycan polymerase RodA in Bacillus subtilis.","publicationDate":"2025","journal":"EMBO Rep.","firstPage":"4587","lastPage":"4606","volume":"26"},"referencePositions":["FUNCTION","INTERACTION WITH RODA","SUBCELLULAR LOCATION","DISRUPTION PHENOTYPE"],"referenceComments":[{"value":"168","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AL009126","properties":[{"key":"ProteinId","value":"CAB14672.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"B69980","properties":[{"key":"EntryName","value":"B69980"}]},{"database":"RefSeq","id":"NP_390608.1","properties":[{"key":"NucleotideSequenceId","value":"NC_000964.3"}]},{"database":"RefSeq","id":"WP_003246156.1","properties":[{"key":"NucleotideSequenceId","value":"NC_000964.3"}]},{"database":"AlphaFoldDB","id":"O32031","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"O32031","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"854294","properties":[{"key":"Interactions","value":"4"}]},{"database":"FunCoup","id":"O32031","properties":[{"key":"Number of interactors","value":"9"}]},{"database":"STRING","id":"224308.BSU27300","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"224308-BSU27300","properties":[{"key":"Description","value":"-"}]},{"database":"DNASU","id":"936614","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblBacteria","id":"CAB14672","properties":[{"key":"ProteinId","value":"CAB14672"},{"key":"GeneId","value":"BSU_27300"}]},{"database":"GeneID","id":"936614","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"bsu:BSU27300","properties":[{"key":"Description","value":"-"}]},{"database":"PATRIC","id":"fig|224308.179.peg.2966","properties":[{"key":"GeneDesignation","value":"-"}]},{"database":"eggNOG","id":"ENOG5032DEE","properties":[{"key":"ToxonomicScope","value":"Bacteria"}]},{"database":"InParanoid","id":"O32031","properties":[{"key":"Description","value":"-"}]},{"database":"OrthoDB","id":"2168558at2","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"BSUB:BSU27300-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000001570","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"AbasyAtlas","id":"O32031","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005886","properties":[{"key":"GoTerm","value":"C:plasma membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"InterPro","id":"IPR009988","properties":[{"key":"EntryName","value":"DUF1510"}]},{"database":"Pfam","id":"PF07423","properties":[{"key":"EntryName","value":"DUF1510"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MSNNQSRYENRDKRRKANLVLNILIAIVSILIVVVAANLFINSPSSKDVSKDSETAQKQESPASGKTEKKSDEDIKDSKKDTSDSEKDSEKSSDSDSKKDDSSSKKDDSDSDSSSDSAGDGLKDAKVTEGGSSSDVEKTYENPDWKAVGTEQTGEHAATYDSSSQDWAEMLKAISYATGVSTDNMTVLWLGNNGSPQDAKGKILDKATGNKYQVTITWVDEKGWKPTKVEKLK","length":233,"molWeight":25334,"crc64":"55C825A2B809D1A3","md5":"B3461CBA531B07029FBC1E603C2FF1CA"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"DISRUPTION PHENOTYPE":1},"countByFeatureType":{"Chain":1,"Transmembrane":1,"Region":1,"Compositional bias":3},"uniParcId":"UPI000006080D"}}