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It prevents the mis-incorporation of both L-serine and glycine containing precursors into the peptidoglycan (PG) sacculi (PubMed:40632566). Acts by hydrolyzing UDP-N-acetylmuramoyl-L-serine (UDP-MurNAc-L-Ser) to release L-serine and UDP-N-acetylmuramoyl-glycine (UDP-MurNAc-Gly) to release glycine, thereby preventing the mis-incorporation of these amino acids into the PG and ensuring that only the correct PG precursor, UDP-MurNAc-L-Ala, is incorporated (PubMed:40632566)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"40632566"}],"value":"The enzyme also shows weak purine nucleoside phosphorylase (PNP) activity in vitro (PubMed:40632566). It can catalyze the phosphorolysis of inosine nucleosides, yielding D-ribose 1-phosphate and the free base hypoxanthine (PubMed:40632566)"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"inosine + phosphate = alpha-D-ribose 1-phosphate + 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