{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"O18195","uniProtkbId":"WASH1_CAEEL","entryAudit":{"firstPublicDate":"2021-09-29","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"1998-01-01","entryVersion":150,"sequenceVersion":1},"annotationScore":5.0,"organism":{"scientificName":"Caenorhabditis elegans","taxonId":6239,"evidences":[{"evidenceCode":"ECO:0000312","source":"Proteomes","id":"UP000001940"}],"lineage":["Eukaryota","Metazoa","Ecdysozoa","Nematoda","Chromadorea","Rhabditida","Rhabditina","Rhabditomorpha","Rhabditoidea","Rhabditidae","Peloderinae","Caenorhabditis"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"A8K0Z3"}],"value":"WASH complex subunit homolog 1"}}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000312","source":"WormBase","id":"Y48E1B.1"}],"value":"ddl-2"},"orfNames":[{"evidences":[{"evidenceCode":"ECO:0000312","source":"WormBase","id":"Y48E1B.1"}],"value":"Y48E1B.1"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"A8K0Z3"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22265419"}],"value":"Acts as a component of the WASH core complex that functions as a nucleation-promoting factor (NPF) at the surface of endosomes, where it recruits and activates the Arp2/3 complex to induce actin polymerization, playing a key role in the fission of tubules that serve as transport intermediates during endosome sorting (By similarity). Acts as a component of the DHIC (ddl-1-containing hsf-1 inhibitory complex) which modulates lifespan by sequestering the heat shock transcription factor hsf-1 to negatively regulate its binding to DNA and its transcriptional activity (PubMed:22265419)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"A8K0Z3"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22265419"}],"value":"Component of the WASH core complex (By similarity). Component of the DHIC (ddl-1-containing hsf-1 inhibitory) complex, which contains at least ddl-1, ddl-2, hsb-1 and hsf-1 (PubMed:22265419). Within the complex, interacts with ddl-1 (PubMed:22265419). Formation of the DHIC may be dependent upon the Insulin/IGF-1-like signaling (IIS) mediated pathway (PubMed:22265419)"}],"commentType":"SUBUNIT"},{"commentType":"INTERACTION","interactions":[{"interactantOne":{"uniProtKBAccession":"O18195","intActId":"EBI-313368"},"interactantTwo":{"uniProtKBAccession":"O01901","geneName":"ddl-1","intActId":"EBI-323542"},"numberOfExperiments":6,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"O18195","intActId":"EBI-313368"},"interactantTwo":{"uniProtKBAccession":"G5EC32","geneName":"sorb-1","intActId":"EBI-325337"},"numberOfExperiments":6,"organismDiffer":false}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22265419"}],"value":"Expressed in several neurons located throughout the body"}],"commentType":"TISSUE SPECIFICITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22265419"}],"value":"RNAi-mediated knockdown positively modulates lifespan; effect abolished in hsf-1 mutant background (PubMed:22265419). Increases resistance to both heat and oxidative stresses (PubMed:22265419). Increases localization to the nucleus and also DNA binding activity of heat-shock transcription factor hsf-1 after heat shock (PubMed:22265419). Increases in transcript levels of heat shock protein genes sim-1, hsp-70, hsp-16.2 and F44E5.5 after heat shock, but not in unstressed conditions (PubMed:22265419). May also moderately decrease levels of post-translationally modified hsf-1 under heat stressed conditions (PubMed:22265419)"}],"commentType":"DISRUPTION PHENOTYPE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the WASH1 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":497,"modifier":"EXACT"}},"description":"WASH complex subunit homolog 1","featureId":"PRO_0000453734"},{"type":"Domain","location":{"start":{"value":390,"modifier":"EXACT"},"end":{"value":412,"modifier":"EXACT"}},"description":"WH2","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00406"}]},{"type":"Region","location":{"start":{"value":306,"modifier":"EXACT"},"end":{"value":497,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional 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