{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"O16374","secondaryAccessions":["G4RVA7"],"uniProtkbId":"GALT1_CAEEL","entryAudit":{"firstPublicDate":"2016-11-02","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2012-01-25","entryVersion":121,"sequenceVersion":4},"annotationScore":5.0,"organism":{"scientificName":"Caenorhabditis elegans","taxonId":6239,"evidences":[{"evidenceCode":"ECO:0000312","source":"Proteomes","id":"UP000001940"}],"lineage":["Eukaryota","Metazoa","Ecdysozoa","Nematoda","Chromadorea","Rhabditida","Rhabditina","Rhabditomorpha","Rhabditoidea","Rhabditidae","Peloderinae","Caenorhabditis"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"19858195"}],"value":"Beta-1,4-galactosyltransferase galt-1"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"19858195"}],"value":"2.4.1.-"}]}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000312","source":"WormBase","id":"M03F8.4"}],"value":"galt-1"},"orfNames":[{"evidences":[{"evidenceCode":"ECO:0000312","source":"WormBase","id":"M03F8.4"}],"value":"M03F8.4"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"19858195"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20062796"}],"value":"Catalyzes the transfer of beta-galactose from UDP-galactose to position 4 of alpha-1,6-linked fucose at the reducing end GlcNAc in N-glycan cores (PubMed:19858195). Involved in susceptibility to the nematotoxic C.cinerea galectin Cgl2, likely by contributing to the synthesis of core alpha-1,6-fucosylated N-glycans to which Cgl2 binds (PubMed:20062796)"}],"commentType":"FUNCTION"},{"commentType":"COFACTOR","cofactors":[{"name":"Mn(2+)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"19858195"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:29035"}}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"19858195"}],"value":"Can also use Fe(2+) and Co(2+)."}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"19858195"}],"value":"Inhibited by EDTA, Cu(2+) and Zn(2+)"}],"commentType":"ACTIVITY REGULATION"},{"commentType":"BIOPHYSICOCHEMICAL PROPERTIES","kineticParameters":{"michaelisConstants":[{"constant":84.0,"unit":"uM","substrate":"UDP-galactose","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"19858195"}]}]}},{"commentType":"SUBCELLULAR LOCATION","note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"19858195"}],"value":"Localizes to vesicles or organelles in coelomocytes"}]},"subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"19858195"}],"value":"Golgi apparatus, Golgi stack membrane","id":"SL-0136"},"topology":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"19858195"}],"value":"Single-pass type II membrane protein","id":"SL-9906"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"19858195"}],"value":"Expressed in intestine and coelomocytes"}],"commentType":"TISSUE SPECIFICITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"19858195"}],"value":"N-glycosylated"}],"commentType":"PTM"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the glycosyltransferase 92 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":425,"modifier":"EXACT"}},"description":"Beta-1,4-galactosyltransferase galt-1","featureId":"PRO_0000438180"},{"type":"Topological domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":8,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000305"}]},{"type":"Transmembrane","location":{"start":{"value":9,"modifier":"EXACT"},"end":{"value":29,"modifier":"EXACT"}},"description":"Helical; Signal-anchor for type II membrane protein","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":30,"modifier":"EXACT"},"end":{"value":425,"modifier":"EXACT"}},"description":"Lumenal","evidences":[{"evidenceCode":"ECO:0000305"}]},{"type":"Domain","location":{"start":{"value":189,"modifier":"EXACT"},"end":{"value":394,"modifier":"EXACT"}},"description":"GT92","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Glycosylation","location":{"start":{"value":109,"modifier":"EXACT"},"end":{"value":109,"modifier":"EXACT"}},"description":"N-linked (GlcNAc...) asparagine","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00498"}],"featureId":""},{"type":"Glycosylation","location":{"start":{"value":152,"modifier":"EXACT"},"end":{"value":152,"modifier":"EXACT"}},"description":"N-linked (GlcNAc...) asparagine","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00498"}],"featureId":""}],"keywords":[{"id":"KW-0325","category":"PTM","name":"Glycoprotein"},{"id":"KW-0328","category":"Molecular function","name":"Glycosyltransferase"},{"id":"KW-0333","category":"Cellular component","name":"Golgi apparatus"},{"id":"KW-0464","category":"Ligand","name":"Manganese"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0735","category":"Domain","name":"Signal-anchor"},{"id":"KW-0808","category":"Molecular function","name":"Transferase"},{"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"id":"KW-1133","category":"Domain","name":"Transmembrane helix"}],"references":[{"referenceNumber":1,"citation":{"id":"9851916","citationType":"journal article","authoringGroup":["The C. elegans sequencing consortium"],"citationCrossReferences":[{"database":"PubMed","id":"9851916"},{"database":"DOI","id":"10.1126/science.282.5396.2012"}],"title":"Genome sequence of the nematode C. elegans: a platform for investigating biology.","publicationDate":"1998","journal":"Science","firstPage":"2012","lastPage":"2018","volume":"282"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000312","source":"Proteomes","id":"UP000001940"}],"value":"Bristol N2","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000312","source":"Proteomes","id":"UP000001940"}]},{"referenceNumber":2,"citation":{"id":"19858195","citationType":"journal article","authors":["Titz A.","Butschi A.","Henrissat B.","Fan Y.Y.","Hennet T.","Razzazi-Fazeli E.","Hengartner M.O.","Wilson I.B.","Kuenzler M.","Aebi M."],"citationCrossReferences":[{"database":"PubMed","id":"19858195"},{"database":"DOI","id":"10.1074/jbc.m109.058354"}],"title":"Molecular basis for galactosylation of core fucose residues in invertebrates: identification of caenorhabditis elegans N-glycan core alpha1,6-fucoside beta1,4-galactosyltransferase GALT-1 as a member of a novel glycosyltransferase family.","publicationDate":"2009","journal":"J. Biol. Chem.","firstPage":"36223","lastPage":"36233","volume":"284"},"referencePositions":["FUNCTION","CATALYTIC ACTIVITY","COFACTOR","ACTIVITY REGULATION","BIOPHYSICOCHEMICAL PROPERTIES","SUBCELLULAR LOCATION","TISSUE SPECIFICITY","GLYCOSYLATION"],"evidences":[{"evidenceCode":"ECO:0000305"}]},{"referenceNumber":3,"citation":{"id":"20062796","citationType":"journal article","authors":["Butschi A.","Titz A.","Waelti M.A.","Olieric V.","Paschinger K.","Noebauer K.","Guo X.","Seeberger P.H.","Wilson I.B.","Aebi M.","Hengartner M.O.","Kuenzler M."],"citationCrossReferences":[{"database":"PubMed","id":"20062796"},{"database":"DOI","id":"10.1371/journal.ppat.1000717"}],"title":"Caenorhabditis elegans N-glycan core beta-galactoside confers sensitivity towards nematotoxic fungal galectin CGL2.","publicationDate":"2010","journal":"PLoS Pathog.","firstPage":"E1000717","lastPage":"E1000717","volume":"6"},"referencePositions":["FUNCTION"],"evidences":[{"evidenceCode":"ECO:0000305"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"BX284605","properties":[{"key":"ProteinId","value":"CCD64148.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"NP_504545.2","properties":[{"key":"NucleotideSequenceId","value":"NM_072144.8"}]},{"database":"AlphaFoldDB","id":"O16374","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"O16374","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"O16374","properties":[{"key":"Number of interactors","value":"32"}]},{"database":"STRING","id":"6239.M03F8.4.1","properties":[{"key":"Description","value":"-"}]},{"database":"CAZy","id":"GT92","properties":[{"key":"FamilyName","value":"Glycosyltransferase Family 92"}]},{"database":"GlyCosmos","id":"O16374","properties":[{"key":"glycosylation","value":"2 sites, No reported glycans"}]},{"database":"PaxDb","id":"6239-M03F8.4.1","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblMetazoa","id":"M03F8.4.1","properties":[{"key":"ProteinId","value":"M03F8.4.1"},{"key":"GeneId","value":"WBGene00019763"}]},{"database":"GeneID","id":"187430","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"cel:CELE_M03F8.4","properties":[{"key":"Description","value":"-"}]},{"database":"UCSC","id":"M03F8.4","properties":[{"key":"OrganismName","value":"c. elegans"}]},{"database":"AGR","id":"WB:WBGene00019763","properties":[{"key":"Description","value":"-"}]},{"database":"CTD","id":"187430","properties":[{"key":"Description","value":"-"}]},{"database":"WormBase","id":"M03F8.4","properties":[{"key":"ProteinId","value":"CE40769"},{"key":"GeneId","value":"WBGene00019763"},{"key":"GeneName","value":"galt-1"}]},{"database":"eggNOG","id":"ENOG502S8SJ","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"HOGENOM","id":"CLU_053206_0_0_1","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"O16374","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"IPIRYRI","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"2526284at2759","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"O16374","properties":[{"key":"Description","value":"-"}]},{"database":"PRO","id":"PR:O16374","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000001940","properties":[{"key":"Component","value":"Chromosome V"}]},{"database":"Bgee","id":"WBGene00019763","properties":[{"key":"ExpressionPatterns","value":"Expressed in embryo and 2 other cell types or tissues"}]},{"database":"GO","id":"GO:0032580","properties":[{"key":"GoTerm","value":"C:Golgi cisterna membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0003831","properties":[{"key":"GoTerm","value":"F:beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase activity"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"23754284"}]},{"database":"GO","id":"GO:0035250","properties":[{"key":"GoTerm","value":"F:UDP-galactosyltransferase activity"},{"key":"GoEvidenceType","value":"IDA:WormBase"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"19858195"}]},{"database":"GO","id":"GO:1901137","properties":[{"key":"GoTerm","value":"P:carbohydrate derivative biosynthetic process"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"23754284"}]},{"database":"GO","id":"GO:0006491","properties":[{"key":"GoTerm","value":"P:N-glycan processing"},{"key":"GoEvidenceType","value":"IDA:WormBase"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"19858195"}]},{"database":"InterPro","id":"IPR008166","properties":[{"key":"EntryName","value":"Glyco_transf_92"}]},{"database":"PANTHER","id":"PTHR21461:SF87","properties":[{"key":"EntryName","value":"GH12965P"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR21461","properties":[{"key":"EntryName","value":"GLYCOSYLTRANSFERASE FAMILY 92 PROTEIN"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01697","properties":[{"key":"EntryName","value":"Glyco_transf_92"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MPRITASKIVLLIALSFCITVIYHFPIATRSSKEYDEYGNEYENVASIESDIKNVRRLLDEVPDPSQNRLQFLKLDEHAFAFSAYTDDRNGNMGYKYVRVLMFITSQDNFSCEINGRKSTDVSLYEFSENHKMKWQMFILNCKLPDGIDFNNVSSVKVIRSTTKQFVDVPIRYRIQDEKIITPDEYDYKMSICVPALFGNGYDAKRIVEFIELNTLQGIEKIYIYTNQKELDGSMKKTLKYYSDNHKITLIDYTLPFREDGVWYHGQLATVTDCLLRNTGITKYTFFNDFDEFFVPVIKSRTLFETISGLFEDPTIGSQRTALKYINAKIKSAPYSLKNIVSEKRIETRFTKCVVRPEMVFEQGIHHTSRVIQDNYKTVSHGGSLLRVYHYKDKKYCCEDESLLKKRHGDQLREKFDSVVGLLDL","length":425,"molWeight":49524,"crc64":"042E77F2ACBD87AE","md5":"E18CC9F9B7D8DACA890E11B20D5A380B"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"COFACTOR":1,"ACTIVITY REGULATION":1,"BIOPHYSICOCHEMICAL PROPERTIES":1,"SUBCELLULAR LOCATION":1,"TISSUE SPECIFICITY":1,"PTM":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Topological domain":2,"Transmembrane":1,"Domain":1,"Glycosylation":2},"uniParcId":"UPI000150BB1E"}}