ID CCL25_HUMAN Reviewed; 150 AA. AC O15444; A1L4J4; A6NI52; A8K9E7; B5MCA5; Q96KJ7; DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot. DT 22-JUL-2008, sequence version 2. DT 02-OCT-2024, entry version 178. DE RecName: Full=C-C motif chemokine 25; DE AltName: Full=Chemokine TECK; DE AltName: Full=Small-inducible cytokine A25; DE AltName: Full=Thymus-expressed chemokine; DE Flags: Precursor; GN Name=CCL25; Synonyms=SCYA25, TECK; OS Homo sapiens (Human). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; OC Homo. OX NCBI_TaxID=9606; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), AND VARIANT MET-104. RC TISSUE=Thymus; RX PubMed=9285413; DOI=10.1016/s1074-7613(00)80531-2; RA Vicari A.P., Figueroa D.J., Hedrick J.A., Foster J.S., Singh K.P., RA Menon S., Copeland N.G., Gilbert D.J., Jenkins N.A., Bacon K.B., RA Zlotnik A.; RT "TECK: a novel CC chemokine specifically expressed by thymic dendritic RT cells and potentially involved in T cell development."; RL Immunity 7:291-301(1997). RN [2] RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). RC TISSUE=Thymus; RA Hieshima K., Nakayama T., Fujisawa R., Izawa D., Yoshie O.; RT "Molecular cloning and characterization of a splicing variant of chemokine RT TECK: a natural antagonist of human TECK."; RL Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases. RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). RC TISSUE=Thymus; RX PubMed=14702039; DOI=10.1038/ng1285; RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S., RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., RA Isogai T., Sugano S.; RT "Complete sequencing and characterization of 21,243 full-length human RT cDNAs."; RL Nat. Genet. 36:40-45(2004). RN [4] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RX PubMed=15057824; DOI=10.1038/nature02399; RA Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., RA Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., RA Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., RA Carrano A.V., Caoile C., Chan Y.M., Christensen M., Cleland C.A., RA Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., RA Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., RA Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., RA Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., RA Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., RA McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., RA Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., RA Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., RA She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., RA Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., RA Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., RA Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., RA Rubin E.M., Lucas S.M.; RT "The DNA sequence and biology of human chromosome 19."; RL Nature 428:529-535(2004). RN [5] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., RA Hunkapiller M.W., Myers E.W., Venter J.C.; RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases. RN [6] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3). RC TISSUE=Lung; RX PubMed=15489334; DOI=10.1101/gr.2596504; RG The MGC Project Team; RT "The status, quality, and expansion of the NIH full-length cDNA project: RT the Mammalian Gene Collection (MGC)."; RL Genome Res. 14:2121-2127(2004). RN [7] RP RECEPTOR INTERACTION. RX PubMed=10229797; RA Zaballos A., Gutierrez J., Varona R., Ardavin C., Marquez G.; RT "Identification of the orphan chemokine receptor GPR-9-6 as CCR9, the RT receptor for the chemokine TECK."; RL J. Immunol. 162:5671-5675(1999). RN [8] RP RECEPTOR INTERACTION. RX PubMed=23341447; DOI=10.1074/jbc.m112.406108; RA Watts A.O., Verkaar F., van der Lee M.M., Timmerman C.A., Kuijer M., RA van Offenbeek J., van Lith L.H., Smit M.J., Leurs R., Zaman G.J., RA Vischer H.F.; RT "Beta-arrestin recruitment and G protein signaling by the atypical human RT chemokine decoy receptor CCX-CKR."; RL J. Biol. Chem. 288:7169-7181(2013). CC -!- FUNCTION: Potentially involved in T-cell development. Recombinant CC protein shows chemotactic activity on thymocytes, macrophages, THP-1 CC cells, and dendritics cells but is inactive on peripheral blood CC lymphocytes and neutrophils. Binds to CCR9. Isoform 2 is an antagonist CC of isoform 1. Binds to atypical chemokine receptor ACKR4 and mediates CC the recruitment of beta-arrestin (ARRB1/2) to ACKR4. CC -!- INTERACTION: CC O15444; Q92583: CCL17; NbExp=2; IntAct=EBI-7783341, EBI-16640146; CC O15444; P13501: CCL5; NbExp=2; IntAct=EBI-7783341, EBI-2848366; CC O15444; P48061: CXCL12; NbExp=2; IntAct=EBI-7783341, EBI-3913254; CC O15444; P02776: PF4; NbExp=3; IntAct=EBI-7783341, EBI-2565740; CC O15444; P10720: PF4V1; NbExp=2; IntAct=EBI-7783341, EBI-1223944; CC -!- SUBCELLULAR LOCATION: Secreted. CC -!- ALTERNATIVE PRODUCTS: CC Event=Alternative splicing; Named isoforms=3; CC Name=1; CC IsoId=O15444-1; Sequence=Displayed; CC Name=2; Synonyms=TECKvar; CC IsoId=O15444-2; Sequence=VSP_001064; CC Name=3; CC IsoId=O15444-3; Sequence=VSP_043199; CC -!- TISSUE SPECIFICITY: Specifically expressed by thymic dendritic cells. CC High levels in thymus and small intestine. CC -!- MISCELLANEOUS: [Isoform 2]: May be produced at very low levels due to a CC premature stop codon in the mRNA, leading to nonsense-mediated mRNA CC decay. {ECO:0000305}. CC -!- SIMILARITY: Belongs to the intercrine beta (chemokine CC) family. CC {ECO:0000305}. CC -!- WEB RESOURCE: Name=Wikipedia; Note=CCL25 entry; CC URL="https://en.wikipedia.org/wiki/CCL25"; CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; U86358; AAB69981.1; -; mRNA. DR EMBL; AB046579; BAB62257.1; -; mRNA. DR EMBL; AK292662; BAF85351.1; -; mRNA. DR EMBL; AC008946; -; NOT_ANNOTATED_CDS; Genomic_DNA. DR EMBL; CH471139; EAW68952.1; -; Genomic_DNA. DR EMBL; BC130561; AAI30562.1; -; mRNA. DR EMBL; BC144463; AAI44464.1; -; mRNA. DR CCDS; CCDS12194.1; -. [O15444-1] DR CCDS; CCDS56080.1; -. [O15444-3] DR RefSeq; NP_001188288.1; NM_001201359.1. [O15444-3] DR RefSeq; NP_005615.2; NM_005624.3. [O15444-1] DR RefSeq; XP_011526479.1; XM_011528177.2. DR RefSeq; XP_016882609.1; XM_017027120.1. DR RefSeq; XP_016882610.1; XM_017027121.1. DR RefSeq; XP_016882611.1; XM_017027122.1. DR AlphaFoldDB; O15444; -. DR SMR; O15444; -. DR BioGRID; 112273; 11. DR DIP; DIP-5883N; -. DR IntAct; O15444; 12. DR MINT; O15444; -. DR STRING; 9606.ENSP00000375086; -. DR iPTMnet; O15444; -. DR PhosphoSitePlus; O15444; -. DR BioMuta; CCL25; -. DR MassIVE; O15444; -. DR PaxDb; 9606-ENSP00000375086; -. DR PeptideAtlas; O15444; -. DR ProteomicsDB; 48672; -. [O15444-1] DR ProteomicsDB; 48673; -. [O15444-2] DR ProteomicsDB; 48674; -. [O15444-3] DR Antibodypedia; 24738; 412 antibodies from 30 providers. DR DNASU; 6370; -. DR Ensembl; ENST00000253451.9; ENSP00000253451.4; ENSG00000131142.14. [O15444-3] DR Ensembl; ENST00000315626.6; ENSP00000324756.6; ENSG00000131142.14. [O15444-1] DR Ensembl; ENST00000390669.7; ENSP00000375086.3; ENSG00000131142.14. [O15444-1] DR Ensembl; ENST00000680450.1; ENSP00000506202.1; ENSG00000131142.14. [O15444-3] DR Ensembl; ENST00000680506.1; ENSP00000505422.1; ENSG00000131142.14. [O15444-1] DR Ensembl; ENST00000680646.1; ENSP00000504875.1; ENSG00000131142.14. [O15444-1] DR Ensembl; ENST00000681526.1; ENSP00000505387.1; ENSG00000131142.14. [O15444-3] DR GeneID; 6370; -. DR KEGG; hsa:6370; -. DR MANE-Select; ENST00000315626.6; ENSP00000324756.6; NM_005624.4; NP_005615.2. DR UCSC; uc002mjc.5; human. [O15444-1] DR AGR; HGNC:10624; -. DR CTD; 6370; -. DR DisGeNET; 6370; -. DR GeneCards; CCL25; -. DR HGNC; HGNC:10624; CCL25. DR HPA; ENSG00000131142; Group enriched (intestine, lymphoid tissue). DR MIM; 602565; gene. DR neXtProt; NX_O15444; -. DR OpenTargets; ENSG00000131142; -. DR PharmGKB; PA35556; -. DR VEuPathDB; HostDB:ENSG00000131142; -. DR eggNOG; ENOG502S8D1; Eukaryota. DR GeneTree; ENSGT01120000271901; -. DR HOGENOM; CLU_113773_0_0_1; -. DR InParanoid; O15444; -. DR OMA; RAWAYRI; -. DR OrthoDB; 5355241at2759; -. DR PhylomeDB; O15444; -. DR TreeFam; TF353160; -. DR PathwayCommons; O15444; -. DR Reactome; R-HSA-380108; Chemokine receptors bind chemokines. DR Reactome; R-HSA-418594; G alpha (i) signalling events. DR SignaLink; O15444; -. DR SIGNOR; O15444; -. DR BioGRID-ORCS; 6370; 11 hits in 1141 CRISPR screens. DR GenomeRNAi; 6370; -. DR Pharos; O15444; Tbio. DR PRO; PR:O15444; -. DR Proteomes; UP000005640; Chromosome 19. DR RNAct; O15444; protein. DR Bgee; ENSG00000131142; Expressed in duodenum and 76 other cell types or tissues. DR ExpressionAtlas; O15444; baseline and differential. DR GO; GO:0005576; C:extracellular region; TAS:Reactome. DR GO; GO:0005615; C:extracellular space; IBA:GO_Central. DR GO; GO:0048020; F:CCR chemokine receptor binding; IBA:GO_Central. DR GO; GO:0031735; F:CCR10 chemokine receptor binding; IDA:BHF-UCL. DR GO; GO:0008009; F:chemokine activity; IDA:UniProtKB. DR GO; GO:0042379; F:chemokine receptor binding; IDA:UniProtKB. DR GO; GO:0005179; F:hormone activity; TAS:ProtInc. DR GO; GO:0061844; P:antimicrobial humoral immune response mediated by antimicrobial peptide; IBA:GO_Central. DR GO; GO:0060326; P:cell chemotaxis; IDA:UniProtKB. DR GO; GO:0007166; P:cell surface receptor signaling pathway; TAS:ProtInc. DR GO; GO:0070098; P:chemokine-mediated signaling pathway; IBA:GO_Central. DR GO; GO:0006935; P:chemotaxis; TAS:ProtInc. DR GO; GO:0048245; P:eosinophil chemotaxis; IBA:GO_Central. DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; IDA:UniProtKB. DR GO; GO:0006955; P:immune response; TAS:ProtInc. DR GO; GO:0006954; P:inflammatory response; IBA:GO_Central. DR GO; GO:1903237; P:negative regulation of leukocyte tethering or rolling; IDA:UniProtKB. DR GO; GO:0030335; P:positive regulation of cell migration; IBA:GO_Central. DR GO; GO:0001954; P:positive regulation of cell-matrix adhesion; IDA:UniProtKB. DR CDD; cd01119; Chemokine_CC_DCCL; 1. DR Gene3D; 2.40.50.40; -; 1. DR InterPro; IPR039809; Chemokine_b/g/d. DR InterPro; IPR034133; Chemokine_CC_DCCL. DR InterPro; IPR001811; Chemokine_IL8-like_dom. DR InterPro; IPR036048; Interleukin_8-like_sf. DR PANTHER; PTHR12015:SF70; C-C MOTIF CHEMOKINE 25; 1. DR PANTHER; PTHR12015; SMALL INDUCIBLE CYTOKINE A; 1. DR Pfam; PF00048; IL8; 1. DR SMART; SM00199; SCY; 1. DR SUPFAM; SSF54117; Interleukin 8-like chemokines; 1. PE 1: Evidence at protein level; KW Alternative splicing; Chemotaxis; Cytokine; Disulfide bond; KW Inflammatory response; Proteomics identification; Reference proteome; KW Secreted; Signal. FT SIGNAL 1..23 FT /evidence="ECO:0000255" FT CHAIN 24..150 FT /note="C-C motif chemokine 25" FT /id="PRO_0000005235" FT DISULFID 30..58 FT /evidence="ECO:0000250" FT DISULFID 31..75 FT /evidence="ECO:0000250" FT VAR_SEQ 65..150 FT /note="FYLPKRHRKVCGNPKSREVQRAMKLLDARNKVFAKLHHNTQTFQAGPHAVKK FT LSSGNSKLSSSKFSNPISSSKRNVSLLISANSGL -> RPSCCKEVEFWKLQVIIIQV FT (in isoform 2)" FT /evidence="ECO:0000303|Ref.2" FT /id="VSP_001064" FT VAR_SEQ 109 FT /note="Missing (in isoform 3)" FT /evidence="ECO:0000303|PubMed:14702039, FT ECO:0000303|PubMed:15489334" FT /id="VSP_043199" FT VARIANT 23 FT /note="T -> A (in dbSNP:rs960173)" FT /id="VAR_044519" FT VARIANT 101 FT /note="H -> R (in dbSNP:rs2032887)" FT /id="VAR_044520" FT VARIANT 104 FT /note="T -> M (in dbSNP:rs1129763)" FT /evidence="ECO:0000269|PubMed:9285413" FT /id="VAR_044521" SQ SEQUENCE 150 AA; 16609 MW; 91D0810F137FBC7B CRC64; MNLWLLACLV AGFLGAWAPA VHTQGVFEDC CLAYHYPIGW AVLRRAWTYR IQEVSGSCNL PAAIFYLPKR HRKVCGNPKS REVQRAMKLL DARNKVFAKL HHNTQTFQAG PHAVKKLSSG NSKLSSSKFS NPISSSKRNV SLLISANSGL //