{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"O14777","secondaryAccessions":["Q6PJX2"],"uniProtkbId":"NDC80_HUMAN","entryAudit":{"firstPublicDate":"2006-09-19","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"1998-01-01","entryVersion":203,"sequenceVersion":1},"annotationScore":5.0,"organism":{"scientificName":"Homo sapiens","commonName":"Human","taxonId":9606,"lineage":["Eukaryota","Metazoa","Chordata","Craniata","Vertebrata","Euteleostomi","Mammalia","Eutheria","Euarchontoglires","Primates","Haplorrhini","Catarrhini","Hominidae","Homo"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Kinetochore protein NDC80 homolog"}},"alternativeNames":[{"fullName":{"value":"Highly expressed in cancer protein"}},{"fullName":{"value":"Kinetochore protein Hec1"},"shortNames":[{"value":"HsHec1"}]},{"fullName":{"value":"Kinetochore-associated protein 2"}},{"fullName":{"value":"Retinoblastoma-associated protein HEC"}}]},"genes":[{"geneName":{"value":"NDC80"},"synonyms":[{"value":"HEC"},{"value":"HEC1"},{"value":"KNTC2"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12351790"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"14654001"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"14699129"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15062103"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15235793"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15239953"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15548592"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16732327"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23085020"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23891108"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25743205"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30409912"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9315664"}],"value":"Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity (PubMed:12351790, PubMed:14654001, PubMed:14699129, PubMed:15062103, PubMed:15235793, PubMed:15239953, PubMed:15548592, PubMed:16732327, PubMed:30409912, PubMed:9315664). Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore (PubMed:15548592, PubMed:30409912). The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules (PubMed:23085020). Plays a role in chromosome congression and is essential for the end-on attachment of the kinetochores to spindle microtubules (PubMed:23891108, PubMed:25743205)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10409732"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10779342"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12386167"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"14602875"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"14699129"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15502821"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15713649"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15824131"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16732327"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22581055"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27156448"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9295362"}],"value":"Component of the NDC80 complex, which consists of NDC80/HEC1, CDCA1, SPBC24 and SPBC25. The NDC80 complex is formed by two subcomplexes composed of NDC80/HEC1-CDCA1 and SPBC24-SPBC25. Each subcomplex is formed by parallel interactions through the coiled-coil domains of individual subunits. Formation of a tetrameric complex is mediated by interactions between the C-terminal regions of both subunits of the NDC80/HEC1-CDCA1 subcomplex and the N-terminal regions of both subunits of the SPBC24-SPBC25 complex. The tetrameric NDC80 complex has an elongated rod-like structure with globular domains at either end. Interacts with isoform 1 of NEK2 and ZWINT specifically during mitosis. Interacts with CENPH and MIS12. May interact with AURKB, PSMC2, PSMC5 and SMC1A. May interact with RB1 during G2 phase and mitosis. Interacts with CKAP5 (PubMed:27156448). Interacts with CDT1; leading to kinetochore localization of CDT1 (PubMed:22581055)"}],"commentType":"SUBUNIT"},{"commentType":"INTERACTION","interactions":[{"interactantOne":{"uniProtKBAccession":"O14777","intActId":"EBI-715849"},"interactantTwo":{"uniProtKBAccession":"Q9Y2J4","geneName":"AMOTL2","intActId":"EBI-746752"},"numberOfExperiments":3,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"O14777","intActId":"EBI-715849"},"interactantTwo":{"uniProtKBAccession":"P18848","geneName":"ATF4","intActId":"EBI-492498"},"numberOfExperiments":8,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"O14777","intActId":"EBI-715849"},"interactantTwo":{"uniProtKBAccession":"P48047","geneName":"ATP5PO","intActId":"EBI-355815"},"numberOfExperiments":3,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"O14777","intActId":"EBI-715849"},"interactantTwo":{"uniProtKBAccession":"Q9Y5K8","geneName":"ATP6V1D","intActId":"EBI-2684998"},"numberOfExperiments":3,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"O14777","intActId":"EBI-715849"},"interactantTwo":{"uniProtKBAccession":"Q8TC20-4","geneName":"CAGE1","intActId":"EBI-11522698"},"numberOfExperiments":3,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"O14777","intActId":"EBI-715849"},"interactantTwo":{"uniProtKBAccession":"Q9P1Z2","geneName":"CALCOCO1","intActId":"EBI-749920"},"numberOfExperiments":6,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"O14777","intActId":"EBI-715849"},"interactantTwo":{"uniProtKBAccession":"Q8TD31-3","geneName":"CCHCR1","intActId":"EBI-10175300"},"numberOfExperiments":6,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"O14777","intActId":"EBI-715849"},"interactantTwo":{"uniProtKBAccession":"P51946","geneName":"CCNH","intActId":"EBI-741406"},"numberOfExperiments":3,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"O14777","intActId":"EBI-715849"},"interactantTwo":{"uniProtKBAccession":"O75909","geneName":"CCNK","intActId":"EBI-739806"},"numberOfExperiments":3,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"O14777","intActId":"EBI-715849"},"interactantTwo":{"uniProtKBAccession":"Q9H211","geneName":"CDT1","intActId":"EBI-456953"},"numberOfExperiments":7,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"O14777","intActId":"EBI-715849"},"interactantTwo":{"uniProtKBAccession":"Q9H3R5","geneName":"CENPH","intActId":"EBI-1003700"},"numberOfExperiments":9,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"O14777","intActId":"EBI-715849"},"interactantTwo":{"uniProtKBAccession":"Q96MT8-3","geneName":"CEP63","intActId":"EBI-11522539"},"numberOfExperiments":3,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"O14777","intActId":"EBI-715849"},"interactantTwo":{"uniProtKBAccession":"Q9UBC2-3","geneName":"EPS15L1","intActId":"EBI-11958621"},"numberOfExperiments":3,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"O14777","intActId":"EBI-715849"},"interactantTwo":{"uniProtKBAccession":"Q8IYI6","geneName":"EXOC8","intActId":"EBI-742102"},"numberOfExperiments":3,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"O14777","intActId":"EBI-715849"},"interactantTwo":{"uniProtKBAccession":"Q8IZT9","geneName":"FAM9C","intActId":"EBI-2870039"},"numberOfExperiments":4,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"O14777","intActId":"EBI-715849"},"interactantTwo":{"uniProtKBAccession":"Q08379","geneName":"GOLGA2","intActId":"EBI-618309"},"numberOfExperiments":3,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"O14777","intActId":"EBI-715849"},"interactantTwo":{"uniProtKBAccession":"Q96CS2","geneName":"HAUS1","intActId":"EBI-2514791"},"numberOfExperiments":3,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"O14777","intActId":"EBI-715849"},"interactantTwo":{"uniProtKBAccession":"O14964","geneName":"HGS","intActId":"EBI-740220"},"numberOfExperiments":6,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"O14777","intActId":"EBI-715849"},"interactantTwo":{"uniProtKBAccession":"O75146-2","geneName":"HIP1R","intActId":"EBI-12292427"},"numberOfExperiments":3,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"O14777","intActId":"EBI-715849"},"interactantTwo":{"uniProtKBAccession":"Q8IY31","geneName":"IFT20","intActId":"EBI-744203"},"numberOfExperiments":5,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"O14777","intActId":"EBI-715849"},"interactantTwo":{"uniProtKBAccession":"Q9BVG8-5","geneName":"KIFC3","intActId":"EBI-14069005"},"numberOfExperiments":3,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"O14777","intActId":"EBI-715849"},"interactantTwo":{"uniProtKBAccession":"Q6P597","geneName":"KLC3","intActId":"EBI-1643885"},"numberOfExperiments":3,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"O14777","intActId":"EBI-715849"},"interactantTwo":{"uniProtKBAccession":"Q7Z3Y8","geneName":"KRT27","intActId":"EBI-3044087"},"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Localizes specifically to the outer plate of the kinetochore (PubMed:14699129)"}]},"subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9315664"}],"value":"Nucleus","id":"SL-0191"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"14699129"}],"value":"Chromosome, centromere, kinetochore","id":"SL-0149"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16732327"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9295362"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9315664"}],"value":"Expression peaks in mitosis"}],"commentType":"DEVELOPMENTAL STAGE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12386167"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"14602875"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30409912"}],"value":"Phosphorylation begins in S phase of the cell cycle and peaks in mitosis. Phosphorylated by NEK2. Also phosphorylated by AURKA and AURKB"}],"commentType":"PTM"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30409912"}],"value":"Acetylated at Lys-53 and Lys-59 by KAT5 during mitosis, promoting robust kinetochore-microtubule attachment (PubMed:30409912). Deacetylated by SIRT1 (PubMed:30409912)"}],"commentType":"PTM"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the NDC80/HEC1 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":642,"modifier":"EXACT"}},"description":"Kinetochore protein NDC80 homolog","featureId":"PRO_0000249550"},{"type":"Region","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":445,"modifier":"EXACT"}},"description":"Interaction with the N-terminus of CDCA1"},{"type":"Region","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":250,"modifier":"EXACT"}},"description":"Nuclear localization"},{"type":"Region","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":74,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":128,"modifier":"EXACT"},"end":{"value":251,"modifier":"EXACT"}},"description":"Interaction with RB1"},{"type":"Region","location":{"start":{"value":251,"modifier":"EXACT"},"end":{"value":618,"modifier":"EXACT"}},"description":"Interaction with NEK2 and ZWINT","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16732327"}]},{"type":"Region","location":{"start":{"value":251,"modifier":"EXACT"},"end":{"value":431,"modifier":"EXACT"}},"description":"Interaction with SMC1A"},{"type":"Region","location":{"start":{"value":361,"modifier":"EXACT"},"end":{"value":547,"modifier":"EXACT"}},"description":"Interaction with PSMC2 and SMC1A"},{"type":"Region","location":{"start":{"value":446,"modifier":"EXACT"},"end":{"value":642,"modifier":"EXACT"}},"description":"Interaction with the C-terminus of CDCA1 and the SPBC24-SPBC25 subcomplex"},{"type":"Coiled coil","location":{"start":{"value":261,"modifier":"EXACT"},"end":{"value":403,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Coiled coil","location":{"start":{"value":458,"modifier":"EXACT"},"end":{"value":642,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Compositional bias","location":{"start":{"value":18,"modifier":"EXACT"},"end":{"value":33,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":63,"modifier":"EXACT"},"end":{"value":74,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Modified residue","location":{"start":{"value":44,"modifier":"EXACT"},"end":{"value":44,"modifier":"EXACT"}},"description":"Phosphoserine","evidences":[{"evidenceCode":"ECO:0007744","source":"PubMed","id":"23186163"}]},{"type":"Modified residue","location":{"start":{"value":53,"modifier":"EXACT"},"end":{"value":53,"modifier":"EXACT"}},"description":"N6-acetyllysine","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30409912"}]},{"type":"Modified residue","location":{"start":{"value":55,"modifier":"EXACT"},"end":{"value":55,"modifier":"EXACT"}},"description":"Phosphoserine","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30409912"}]},{"type":"Modified residue","location":{"start":{"value":59,"modifier":"EXACT"},"end":{"value":59,"modifier":"EXACT"}},"description":"N6-acetyllysine","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30409912"}]},{"type":"Modified residue","location":{"start":{"value":62,"modifier":"EXACT"},"end":{"value":62,"modifier":"EXACT"}},"description":"Phosphoserine","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30409912"}]},{"type":"Modified residue","location":{"start":{"value":69,"modifier":"EXACT"},"end":{"value":69,"modifier":"EXACT"}},"description":"Phosphoserine","evidences":[{"evidenceCode":"ECO:0007744","source":"PubMed","id":"20068231"},{"evidenceCode":"ECO:0007744","source":"PubMed","id":"23186163"}]},{"type":"Modified residue","location":{"start":{"value":165,"modifier":"EXACT"},"end":{"value":165,"modifier":"EXACT"}},"description":"Phosphoserine; 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