{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"O13046","uniProtkbId":"WDHD1_XENLA","entryAudit":{"firstPublicDate":"2003-02-22","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"1997-07-01","entryVersion":125,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Xenopus laevis","commonName":"African clawed frog","taxonId":8355,"lineage":["Eukaryota","Metazoa","Chordata","Craniata","Vertebrata","Euteleostomi","Amphibia","Batrachia","Anura","Pipoidea","Pipidae","Xenopodinae","Xenopus","Xenopus"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"WD repeat and HMG-box DNA-binding protein 1"}},"alternativeNames":[{"fullName":{"value":"Acidic nucleoplasmic DNA-binding protein 1"},"shortNames":[{"value":"And-1"}]}]},"genes":[{"geneName":{"value":"wdhd1"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O75717"}],"value":"Core replisome component that acts as a replication initiation factor. Binds directly to the CMG complex and functions as a hub to recruit additional proteins to the replication fork"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"9175701"}],"value":"Homodimer"}],"commentType":"SUBUNIT"},{"commentType":"INTERACTION","interactions":[{"interactantOne":{"uniProtKBAccession":"O13046","intActId":"EBI-3510652"},"interactantTwo":{"uniProtKBAccession":"Q9DE46","geneName":"pola1","intActId":"EBI-3645423"},"numberOfExperiments":2,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"O13046","intActId":"EBI-3510652"},"interactantTwo":{"uniProtKBAccession":"Q0IHI4","geneName":"tipin","intActId":"EBI-7570880"},"numberOfExperiments":5,"organismDiffer":false}]},{"commentType":"SUBCELLULAR LOCATION","note":{"texts":[{"value":"Transiently cytoplasmic during mitosis"}]},"subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9175701"}],"value":"Nucleus, nucleoplasm","id":"SL-0190"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9175701"}],"value":"Cytoplasm","id":"SL-0086"}}]},{"texts":[{"value":"Found in oocytes and in various other cells"}],"commentType":"TISSUE SPECIFICITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1127,"modifier":"EXACT"}},"description":"WD repeat and HMG-box DNA-binding protein 1","featureId":"PRO_0000051340"},{"type":"Repeat","location":{"start":{"value":11,"modifier":"EXACT"},"end":{"value":50,"modifier":"EXACT"}},"description":"WD 1"},{"type":"Repeat","location":{"start":{"value":52,"modifier":"EXACT"},"end":{"value":91,"modifier":"EXACT"}},"description":"WD 2"},{"type":"Repeat","location":{"start":{"value":92,"modifier":"EXACT"},"end":{"value":131,"modifier":"EXACT"}},"description":"WD 3"},{"type":"Repeat","location":{"start":{"value":134,"modifier":"EXACT"},"end":{"value":173,"modifier":"EXACT"}},"description":"WD 4"},{"type":"Repeat","location":{"start":{"value":184,"modifier":"EXACT"},"end":{"value":223,"modifier":"EXACT"}},"description":"WD 5"},{"type":"Repeat","location":{"start":{"value":228,"modifier":"EXACT"},"end":{"value":267,"modifier":"EXACT"}},"description":"WD 6"},{"type":"Repeat","location":{"start":{"value":271,"modifier":"EXACT"},"end":{"value":310,"modifier":"EXACT"}},"description":"WD 7"},{"type":"DNA binding","location":{"start":{"value":1013,"modifier":"EXACT"},"end":{"value":1076,"modifier":"EXACT"}},"description":"HMG box","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00267"}]},{"type":"Region","location":{"start":{"value":811,"modifier":"EXACT"},"end":{"value":1013,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":1064,"modifier":"EXACT"},"end":{"value":1127,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":819,"modifier":"EXACT"},"end":{"value":829,"modifier":"EXACT"}},"description":"Acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":846,"modifier":"EXACT"},"end":{"value":857,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":858,"modifier":"EXACT"},"end":{"value":877,"modifier":"EXACT"}},"description":"Acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":881,"modifier":"EXACT"},"end":{"value":891,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":918,"modifier":"EXACT"},"end":{"value":937,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":948,"modifier":"EXACT"},"end":{"value":960,"modifier":"EXACT"}},"description":"Low complexity","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":1087,"modifier":"EXACT"},"end":{"value":1100,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]}],"keywords":[{"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"id":"KW-0238","category":"Molecular function","name":"DNA-binding"},{"id":"KW-0539","category":"Cellular component","name":"Nucleus"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0677","category":"Domain","name":"Repeat"},{"id":"KW-0853","category":"Domain","name":"WD repeat"}],"references":[{"referenceNumber":1,"citation":{"id":"9175701","citationType":"journal article","authors":["Koehler A.","Schmidt-Zachmann M.S.","Franke W.W."],"citationCrossReferences":[{"database":"PubMed","id":"9175701"},{"database":"DOI","id":"10.1242/jcs.110.9.1051"}],"title":"AND-1, a natural chimeric DNA-binding protein, combines an HMG-box with regulatory WD-repeats.","publicationDate":"1997","journal":"J. Cell Sci.","firstPage":"1051","lastPage":"1062","volume":"110"},"referencePositions":["NUCLEOTIDE SEQUENCE [MRNA]","SUBCELLULAR LOCATION","SUBUNIT","DNA-BINDING"],"referenceComments":[{"value":"Oocyte","type":"TISSUE"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"X98884","properties":[{"key":"ProteinId","value":"CAA67387.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"PIR","id":"T30334","properties":[{"key":"EntryName","value":"T30334"}]},{"database":"AlphaFoldDB","id":"O13046","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"O13046","properties":[{"key":"Description","value":"-"}]},{"database":"IntAct","id":"O13046","properties":[{"key":"Interactions","value":"5"}]},{"database":"MINT","id":"O13046","properties":[{"key":"Description","value":"-"}]},{"database":"AGR","id":"Xenbase:XB-GENE-1217712","properties":[{"key":"Description","value":"-"}]},{"database":"Xenbase","id":"XB-GENE-1217712","properties":[{"key":"GeneName","value":"wdhd1.L"}]},{"database":"OrthoDB","id":"427368at2759","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000186698","properties":[{"key":"Component","value":"Unplaced"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0043596","properties":[{"key":"GoTerm","value":"C:nuclear replication fork"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0005654","properties":[{"key":"GoTerm","value":"C:nucleoplasm"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0003682","properties":[{"key":"GoTerm","value":"F:chromatin binding"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0003677","properties":[{"key":"GoTerm","value":"F:DNA binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0006281","properties":[{"key":"GoTerm","value":"P:DNA repair"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0006261","properties":[{"key":"GoTerm","value":"P:DNA-templated DNA replication"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0000278","properties":[{"key":"GoTerm","value":"P:mitotic cell cycle"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"CDD","id":"cd21993","properties":[{"key":"EntryName","value":"HMG-box_WDHD1"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd00200","properties":[{"key":"EntryName","value":"WD40"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.10.30.10:FF:000028","properties":[{"key":"EntryName","value":"WD repeat and HMG-box DNA-binding protein 1"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"2.130.10.10:FF:001715","properties":[{"key":"EntryName","value":"WD repeat and HMG-box DNA-binding protein 1"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.30.10","properties":[{"key":"EntryName","value":"High mobility group box domain"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.130.10.10","properties":[{"key":"EntryName","value":"YVTN repeat-like/Quinoprotein amine dehydrogenase"},{"key":"MatchStatus","value":"2"}]},{"database":"InterPro","id":"IPR055339","properties":[{"key":"EntryName","value":"HMG-box_WDHD1"}]},{"database":"InterPro","id":"IPR009071","properties":[{"key":"EntryName","value":"HMG_box_dom"}]},{"database":"InterPro","id":"IPR036910","properties":[{"key":"EntryName","value":"HMG_box_dom_sf"}]},{"database":"InterPro","id":"IPR015943","properties":[{"key":"EntryName","value":"WD40/YVTN_repeat-like_dom_sf"}]},{"database":"InterPro","id":"IPR019775","properties":[{"key":"EntryName","value":"WD40_repeat_CS"}]},{"database":"InterPro","id":"IPR036322","properties":[{"key":"EntryName","value":"WD40_repeat_dom_sf"}]},{"database":"InterPro","id":"IPR001680","properties":[{"key":"EntryName","value":"WD40_rpt"}]},{"database":"InterPro","id":"IPR057646","properties":[{"key":"EntryName","value":"WD40_WDHD1_1st"}]},{"database":"InterPro","id":"IPR022100","properties":[{"key":"EntryName","value":"WDHD1/CFT4_beta-prop_2nd"}]},{"database":"InterPro","id":"IPR048591","properties":[{"key":"EntryName","value":"WDHD1/CFT4_hel"}]},{"database":"PANTHER","id":"PTHR19932","properties":[{"key":"EntryName","value":"WD REPEAT AND HMG-BOX DNA BINDING PROTEIN"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR19932:SF10","properties":[{"key":"EntryName","value":"WD REPEAT AND HMG-BOX DNA-BINDING PROTEIN 1"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF20946","properties":[{"key":"EntryName","value":"Ctf4_C"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF24815","properties":[{"key":"EntryName","value":"HMG_WDHD1"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF12341","properties":[{"key":"EntryName","value":"Mcl1_mid"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF24817","properties":[{"key":"EntryName","value":"WD40_WDHD1_1st"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00398","properties":[{"key":"EntryName","value":"HMG"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00320","properties":[{"key":"EntryName","value":"WD40"},{"key":"MatchStatus","value":"5"}]},{"database":"SUPFAM","id":"SSF47095","properties":[{"key":"EntryName","value":"HMG-box"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF50978","properties":[{"key":"EntryName","value":"WD40 repeat-like"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS50118","properties":[{"key":"EntryName","value":"HMG_BOX_2"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00678","properties":[{"key":"EntryName","value":"WD_REPEATS_1"},{"key":"MatchStatus","value":"2"}]},{"database":"PROSITE","id":"PS50082","properties":[{"key":"EntryName","value":"WD_REPEATS_2"},{"key":"MatchStatus","value":"3"}]},{"database":"PROSITE","id":"PS50294","properties":[{"key":"EntryName","value":"WD_REPEATS_REGION"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MPAIKKNMRYGHPEGHTDVCFDDSGNFLVTCGSDGDIRIWESLDDDDPKSISIGEKAYSFALKNGKVVTAASNNAIQLHTFPDGEPDGILTRFTTNANHVVFNTDGTRIAAGSGDFLVKVLQVEDSTQQKTLRGHSAPVLSVSFDPKDIYLASASCDGSVRIWKISDQTCEAVLPLLEKCNDVFNAKSICRLAWQPKSGKFVAIPVGKAVHLYDRDSLKNICTLSDDFITQPVNIVAWSPCGQYLVAGSVDGCIVAWNIATKACLERIKHEKGYTICALAWHPHLPQIAYTDNEGNLGLLEDVCQGDVKQPSAKVSSAETKDYDELFDGDDDEDFLNGDMIGHEGAVNDEDDDDNFTALTGRPRNRGAIFDDDISSDVPSLKLVGNENPVVEDDQASSVQNFTSVASVKLSYNGPMPTPQQKPFQSGSTPVHLMHRFMVWNSVGVIRCYNDEQDNAIDVEFHDTSIHHAIHLTNSLNHTLADVSQEAVLLACETTEELASKLQCLHFSSWDTSKEWMVDMPKGENIQAICLGQGWVACATSALLIRIFSVGGVQKELISLFGPVVCMASHGEQLIVVYHRGMGFDGDQCLGVQLLELGKKKKQVLHGDPLPLSRKSYLSWLGFTAEGSPCYVDSEGIVRLLNRSLGDTWVPICNTREHCKGKSDHYWVVGIHENPQQVRCIPCKGSRFPPTLPRPAVAVLPFNLPYCQITTEKGQMEEQYWRSQIFSNHSDYLSKHGYECDENFKAEAQKMQQELLMKMFALSCKLEREFRCMELAEFMTQNVMNLAIKYASRSKRLILAQRLSEMALEKAAEQASAEQNEEEDEEEEDFRSRLTAGYSRTATEWGDSRAKPVKQDQYEENNEEEMEEEEKEQEEALESNTPTANPFNKSVKTPDVSESKLGAILSSNQGRVNPFKVSASQKSPAFASNSSRSTSILDNMGKFPRKPSASGSPSTSKSDSVIIKPLAPKAKSKQGQATLFHSVQAKPIAKKTTEEKKAVPTPPKAAADIAENKKPKTGFQLWLDENRPSILSENAGLDESEIIKEGMSRFRMLTSEERMLWTEKAKGDYPGEDGADAKKRKRPEQENMASNGCPQENTDSGIAAAKKHKPLGQSANNKLSAFAFKKE","length":1127,"molWeight":124650,"crc64":"4F4C4A3FC7422082","md5":"07BF4A89E4C3BF356948BCF11E9F3969"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBUNIT":1,"INTERACTION":2,"SUBCELLULAR LOCATION":1,"TISSUE SPECIFICITY":1},"countByFeatureType":{"Chain":1,"Repeat":7,"DNA binding":1,"Region":2,"Compositional bias":7},"uniParcId":"UPI0000125AC7"}}