{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"O05523","uniProtkbId":"TATCY_BACSU","entryAudit":{"firstPublicDate":"1997-11-01","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"1997-07-01","entryVersion":148,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Bacillus subtilis (strain 168)","taxonId":224308,"lineage":["Bacteria","Bacillati","Bacillota","Bacilli","Caryophanales","Bacillaceae","Bacillus"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00902"}],"value":"Sec-independent protein translocase protein TatCy"}}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00902"}],"value":"tatC2"},"synonyms":[{"value":"tatCy"},{"value":"ydiJ"}],"orderedLocusNames":[{"value":"BSU05990"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00902"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"19049517"}],"value":"Part of the twin-arginine translocation (Tat) system that transports large folded proteins containing a characteristic twin-arginine motif in their signal peptide across membranes. Required for YwbN secretion"}],"commentType":"FUNCTION"},{"texts":[{"value":"Forms a complex with TatAy. Two types of complexes exist: one composed of TatAy and TatCy, and another composed only of TatAy"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00902"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"19049517"}],"value":"Cell membrane","id":"SL-0039"},"topology":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00902"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"19049517"}],"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"texts":[{"value":"B.subtilis possesses two minimal, substrate-specific, Tat translocases: TatAd-TatCd and TatAy-TatCy, each one composed of a TatA and a TatC protein. TatA is bifunctional and performs the function of both the TatA and TatB proteins of Gram-negative organisms"}],"commentType":"MISCELLANEOUS"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00902"}],"value":"Belongs to the TatC family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":254,"modifier":"EXACT"}},"description":"Sec-independent protein translocase protein TatCy","featureId":"PRO_0000098096"},{"type":"Transmembrane","location":{"start":{"value":24,"modifier":"EXACT"},"end":{"value":44,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00902"}]},{"type":"Transmembrane","location":{"start":{"value":67,"modifier":"EXACT"},"end":{"value":87,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00902"}]},{"type":"Transmembrane","location":{"start":{"value":112,"modifier":"EXACT"},"end":{"value":132,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00902"}]},{"type":"Transmembrane","location":{"start":{"value":157,"modifier":"EXACT"},"end":{"value":177,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00902"}]},{"type":"Transmembrane","location":{"start":{"value":187,"modifier":"EXACT"},"end":{"value":207,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00902"}]},{"type":"Transmembrane","location":{"start":{"value":212,"modifier":"EXACT"},"end":{"value":232,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00902"}]}],"keywords":[{"id":"KW-1003","category":"Cellular component","name":"Cell membrane"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-0653","category":"Biological process","name":"Protein transport"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0811","category":"Biological process","name":"Translocation"},{"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"id":"KW-1133","category":"Domain","name":"Transmembrane helix"},{"id":"KW-0813","category":"Biological process","name":"Transport"}],"references":[{"referenceNumber":1,"citation":{"id":"9202461","citationType":"journal article","authors":["Sadaie Y.","Yata K.","Fujita M.","Sagai H.","Itaya M.","Kasahara Y.","Ogasawara N."],"citationCrossReferences":[{"database":"PubMed","id":"9202461"},{"database":"DOI","id":"10.1099/00221287-143-6-1861"}],"title":"Nucleotide sequence and analysis of the phoB-rrnE-groESL region of the Bacillus subtilis chromosome.","publicationDate":"1997","journal":"Microbiology","firstPage":"1861","lastPage":"1866","volume":"143"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"],"referenceComments":[{"value":"168 / JH642","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"9384377","citationType":"journal article","authors":["Kunst F.","Ogasawara N.","Moszer I.","Albertini A.M.","Alloni G.","Azevedo V.","Bertero M.G.","Bessieres P.","Bolotin A.","Borchert S.","Borriss R.","Boursier L.","Brans A.","Braun M.","Brignell S.C.","Bron S.","Brouillet S.","Bruschi C.V.","Caldwell B.","Capuano V.","Carter N.M.","Choi S.-K.","Codani J.-J.","Connerton I.F.","Cummings N.J.","Daniel R.A.","Denizot F.","Devine K.M.","Duesterhoeft A.","Ehrlich S.D.","Emmerson P.T.","Entian K.-D.","Errington J.","Fabret C.","Ferrari E.","Foulger D.","Fritz C.","Fujita M.","Fujita Y.","Fuma S.","Galizzi A.","Galleron N.","Ghim S.-Y.","Glaser P.","Goffeau A.","Golightly E.J.","Grandi G.","Guiseppi G.","Guy B.J.","Haga K.","Haiech J.","Harwood C.R.","Henaut A.","Hilbert H.","Holsappel S.","Hosono S.","Hullo M.-F.","Itaya M.","Jones L.-M.","Joris B.","Karamata D.","Kasahara Y.","Klaerr-Blanchard M.","Klein C.","Kobayashi Y.","Koetter P.","Koningstein G.","Krogh S.","Kumano M.","Kurita K.","Lapidus A.","Lardinois S.","Lauber J.","Lazarevic V.","Lee S.-M.","Levine A.","Liu H.","Masuda S.","Mauel C.","Medigue C.","Medina N.","Mellado R.P.","Mizuno M.","Moestl D.","Nakai S.","Noback M.","Noone D.","O'Reilly M.","Ogawa K.","Ogiwara A.","Oudega B.","Park S.-H.","Parro V.","Pohl T.M.","Portetelle D.","Porwollik S.","Prescott A.M.","Presecan E.","Pujic P.","Purnelle B.","Rapoport G.","Rey M.","Reynolds S.","Rieger M.","Rivolta C.","Rocha E.","Roche B.","Rose M.","Sadaie Y.","Sato T.","Scanlan E.","Schleich S.","Schroeter R.","Scoffone F.","Sekiguchi J.","Sekowska A.","Seror S.J.","Serror P.","Shin B.-S.","Soldo B.","Sorokin A.","Tacconi E.","Takagi T.","Takahashi H.","Takemaru K.","Takeuchi M.","Tamakoshi A.","Tanaka T.","Terpstra P.","Tognoni A.","Tosato V.","Uchiyama S.","Vandenbol M.","Vannier F.","Vassarotti A.","Viari A.","Wambutt R.","Wedler E.","Wedler H.","Weitzenegger T.","Winters P.","Wipat A.","Yamamoto H.","Yamane K.","Yasumoto K.","Yata K.","Yoshida K.","Yoshikawa H.-F.","Zumstein E.","Yoshikawa H.","Danchin A."],"citationCrossReferences":[{"database":"PubMed","id":"9384377"},{"database":"DOI","id":"10.1038/36786"}],"title":"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis.","publicationDate":"1997","journal":"Nature","firstPage":"249","lastPage":"256","volume":"390"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"168","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"11007775","citationType":"journal article","authors":["Jongbloed J.D.H.","Martin U.","Antelmann H.","Hecker M.","Tjalsma H.","Venema G.","Bron S.","van Dijl J.M.","Mueller J."],"citationCrossReferences":[{"database":"PubMed","id":"11007775"},{"database":"DOI","id":"10.1074/jbc.m004887200"}],"title":"TatC is a specificity determinant for protein secretion via the twin-arginine translocation pathway.","publicationDate":"2000","journal":"J. Biol. Chem.","firstPage":"41350","lastPage":"41357","volume":"275"},"referencePositions":["IDENTIFICATION OF THE TAT GENES"]},{"referenceNumber":4,"citation":{"id":"15554971","citationType":"journal article","authors":["Jongbloed J.D.H.","Grieger U.","Antelmann H.","Hecker M.","Nijland R.","Bron S.","van Dijl J.M."],"citationCrossReferences":[{"database":"PubMed","id":"15554971"},{"database":"DOI","id":"10.1111/j.1365-2958.2004.04341.x"}],"title":"Two minimal Tat translocases in Bacillus.","publicationDate":"2004","journal":"Mol. Microbiol.","firstPage":"1319","lastPage":"1325","volume":"54"},"referencePositions":["EXPORT OF THE YWBN PROTEIN","CHARACTERIZATION OF THE TWO TAT TRANSLOCASE SYSTEMS"]},{"referenceNumber":5,"citation":{"id":"19049517","citationType":"journal article","authors":["Barnett J.P.","van der Ploeg R.","Eijlander R.T.","Nenninger A.","Mendel S.","Rozeboom R.","Kuipers O.P.","van Dijl J.M.","Robinson C."],"citationCrossReferences":[{"database":"PubMed","id":"19049517"},{"database":"DOI","id":"10.1111/j.1742-4658.2008.06776.x"}],"title":"The twin-arginine translocation (Tat) systems from Bacillus subtilis display a conserved mode of complex organization and similar substrate recognition requirements.","publicationDate":"2009","journal":"FEBS J.","firstPage":"232","lastPage":"243","volume":"276"},"referencePositions":["FUNCTION","SUBCELLULAR LOCATION"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"D88802","properties":[{"key":"ProteinId","value":"BAA19723.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AL009126","properties":[{"key":"ProteinId","value":"CAB12418.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"C69787","properties":[{"key":"EntryName","value":"C69787"}]},{"database":"RefSeq","id":"NP_388480.1","properties":[{"key":"NucleotideSequenceId","value":"NC_000964.3"}]},{"database":"RefSeq","id":"WP_010886426.1","properties":[{"key":"NucleotideSequenceId","value":"NC_000964.3"}]},{"database":"AlphaFoldDB","id":"O05523","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"O05523","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"O05523","properties":[{"key":"Number of interactors","value":"521"}]},{"database":"IntAct","id":"O05523","properties":[{"key":"Interactions","value":"22"}]},{"database":"STRING","id":"224308.BSU05990","properties":[{"key":"Description","value":"-"}]},{"database":"TCDB","id":"2.A.64.3.2","properties":[{"key":"FamilyName","value":"the twin arginine targeting (tat) family"}]},{"database":"PaxDb","id":"224308-BSU05990","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblBacteria","id":"CAB12418","properties":[{"key":"ProteinId","value":"CAB12418"},{"key":"GeneId","value":"BSU_05990"}]},{"database":"GeneID","id":"938010","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"bsu:BSU05990","properties":[{"key":"Description","value":"-"}]},{"database":"PATRIC","id":"fig|224308.43.peg.629","properties":[{"key":"GeneDesignation","value":"-"}]},{"database":"eggNOG","id":"COG0805","properties":[{"key":"ToxonomicScope","value":"Bacteria"}]},{"database":"InParanoid","id":"O05523","properties":[{"key":"Description","value":"-"}]},{"database":"OrthoDB","id":"9777044at2","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"O05523","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"BSUB:BSU05990-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000001570","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"AbasyAtlas","id":"O05523","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0033281","properties":[{"key":"GoTerm","value":"C:TAT protein transport complex"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0009977","properties":[{"key":"GoTerm","value":"F:proton motive force dependent protein transmembrane transporter activity"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0065002","properties":[{"key":"GoTerm","value":"P:intracellular protein transmembrane transport"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0043953","properties":[{"key":"GoTerm","value":"P:protein transport by the Tat complex"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"HAMAP","id":"MF_00902","properties":[{"key":"EntryName","value":"TatC"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR019820","properties":[{"key":"EntryName","value":"Sec-indep_translocase_CS"}]},{"database":"InterPro","id":"IPR002033","properties":[{"key":"EntryName","value":"TatC"}]},{"database":"NCBIfam","id":"TIGR00945","properties":[{"key":"EntryName","value":"tatC"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR30371","properties":[{"key":"EntryName","value":"SEC-INDEPENDENT PROTEIN TRANSLOCASE PROTEIN TATC"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR30371:SF0","properties":[{"key":"EntryName","value":"SEC-INDEPENDENT PROTEIN TRANSLOCASE PROTEIN TATC, CHLOROPLASTIC-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00902","properties":[{"key":"EntryName","value":"TatC"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR01840","properties":[{"key":"EntryName","value":"TATCFAMILY"}]},{"database":"PROSITE","id":"PS01218","properties":[{"key":"EntryName","value":"TATC"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MTRMKVNQMSLLEHIAELRKRLLIVALAFVVFFIAGFFLAKPIIVYLQETDEAKQLTLNAFNLTDPLYVFMQFAFIIGIVLTSPVILYQLWAFVSPGLYEKERKVTLSYIPVSILLFLAGLSFSYYILFPFVVDFMKRISQDLNVNQVIGINEYFHFLLQLTIPFGLLFQMPVILMFLTRLGIVTPMFLAKIRKYAYFTLLVIAALITPPELLSHMMVTVPLLILYEISILISKAAYRKAQKSSAADRDVSSGQ","length":254,"molWeight":29056,"crc64":"803DC23BCB6B24A7","md5":"9546DED1957E15BF2446EEE1102933F8"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"MISCELLANEOUS":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Transmembrane":6},"uniParcId":"UPI00000540C1"}}