{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"O01901","uniProtkbId":"WASC3_CAEEL","entryAudit":{"firstPublicDate":"2000-05-30","lastAnnotationUpdateDate":"2026-06-10","lastSequenceUpdateDate":"1997-07-01","entryVersion":136,"sequenceVersion":1},"annotationScore":5.0,"organism":{"scientificName":"Caenorhabditis elegans","taxonId":6239,"lineage":["Eukaryota","Metazoa","Ecdysozoa","Nematoda","Chromadorea","Rhabditida","Rhabditina","Rhabditomorpha","Rhabditoidea","Rhabditidae","Peloderinae","Caenorhabditis"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9Y3C0"}],"value":"WASH complex subunit homolog 3"}},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000312","source":"WormBase","id":"F59E12.10"}],"value":"Daf-16-dependent longevity protein 1"}}]},"genes":[{"geneName":{"value":"ddl-1"},"orfNames":[{"value":"F59E12.10"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9Y3C0"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22265419"}],"value":"Acts as a component of the WASH core complex that functions as a nucleation-promoting factor (NPF) at the surface of endosomes, where it recruits and activates the Arp2/3 complex to induce actin polymerization, playing a key role in the fission of tubules that serve as transport intermediates during endosome sorting (By similarity). Acts as a component of the DHIC (ddl-1-containing hsf-1 inhibitory complex) which modulates lifespan by sequestering the heat-shock transcription factor hsf-1 to negatively regulate its binding to DNA and its transcriptional activity (PubMed:22265419)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9Y3C0"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22265419"}],"value":"Probable component of the WASH complex (By similarity). Component of the DHIC (ddl-1-containing hsf-1 inhibitory complex), which contains at least ddl-1, ddl-2, hsb-1 and hsf-1 (PubMed:22265419). Within the complex, interacts with ddl-2 (PubMed:22265419). Within the complex, interacts with hsb-1 (PubMed:22265419). Within the complex, interacts with hsf-1 (PubMed:22265419). Formation of the DHIC may be dependent upon the Insulin/IGF-1-like signaling (IIS) mediated pathway (PubMed:22265419)"}],"commentType":"SUBUNIT"},{"commentType":"INTERACTION","interactions":[{"interactantOne":{"uniProtKBAccession":"O01901","intActId":"EBI-323542"},"interactantTwo":{"uniProtKBAccession":"O18195","geneName":"ddl-2","intActId":"EBI-313368"},"numberOfExperiments":6,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"O01901","intActId":"EBI-323542"},"interactantTwo":{"uniProtKBAccession":"Q9U3B7","geneName":"hsb-1","intActId":"EBI-311898"},"numberOfExperiments":5,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"O01901","intActId":"EBI-323542"},"interactantTwo":{"uniProtKBAccession":"G5EFT5","geneName":"hsf-1","intActId":"EBI-2916699"},"numberOfExperiments":3,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"O01901","intActId":"EBI-323542"},"interactantTwo":{"uniProtKBAccession":"G5ECG0","geneName":"tac-1","intActId":"EBI-320612"},"numberOfExperiments":3,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"O01901","intActId":"EBI-323542"},"interactantTwo":{"uniProtKBAccession":"Q9XVK6","geneName":"wve-1","intActId":"EBI-312105"},"numberOfExperiments":3,"organismDiffer":false}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22265419"}],"value":"Expressed in pharynx, intestine, body wall muscles, vulva muscles, spermatheca, and several head and tail neurons"}],"commentType":"TISSUE SPECIFICITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22265419"}],"value":"Phosphorylated (PubMed:22265419). Phosphorylation on Thr-182 may promote DHIC complex dissociation and consequently the activation of heat-shock transcription factor hsf-1 (PubMed:22265419). Phosphorylation is modulated by the Insulin/IGF-1-like signaling (IIS) mediated pathway (PubMed:22265419)"}],"commentType":"PTM"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22265419"}],"value":"RNAi-mediated knockdown positively modulates lifespan; effect abolished in hsf-1 mutant background (PubMed:22265419). Increases resistance to both heat and oxidative stresses (PubMed:22265419). Increases localization to the nucleus and also DNA binding activity of heat-shock transcription factor hsf-1 both before and after heat shock (PubMed:22265419). Increases in transcript levels of heat-shock protein genes sim-1, hsp-70, hsp-16.2 and F44E5.5 after heat shock, but not in unstressed conditions (PubMed:22265419). May also increase levels of post-translationally modified hsf-1 under heat stressed and unstressed conditions (PubMed:22265419)"}],"commentType":"DISRUPTION PHENOTYPE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the CCDC53 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":189,"modifier":"EXACT"}},"description":"WASH complex subunit homolog 3","featureId":"PRO_0000065388"},{"type":"Region","location":{"start":{"value":76,"modifier":"EXACT"},"end":{"value":101,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":150,"modifier":"EXACT"},"end":{"value":189,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Coiled 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