{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"O01658","secondaryAccessions":["G8XYY5"],"uniProtkbId":"NO66_CAEEL","entryAudit":{"firstPublicDate":"2010-01-19","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2001-10-01","entryVersion":149,"sequenceVersion":2},"annotationScore":5.0,"organism":{"scientificName":"Caenorhabditis elegans","taxonId":6239,"lineage":["Eukaryota","Metazoa","Ecdysozoa","Nematoda","Chromadorea","Rhabditida","Rhabditina","Rhabditomorpha","Rhabditoidea","Rhabditidae","Peloderinae","Caenorhabditis"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"value":"Bifunctional lysine-specific demethylase and histidyl-hydroxylase NO66"},"ecNumbers":[{"value":"1.14.11.-"},{"value":"1.14.11.27"}]},"alternativeNames":[{"fullName":{"value":"Histone lysine demethylase NO66"}},{"fullName":{"value":"Jumanjic domain protein 1"}}]},"genes":[{"geneName":{"value":"jmjc-1"},"orfNames":[{"value":"T28F2.4"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20057358"}],"value":"Oxygenase that can act as both a histone lysine demethylase and a ribosomal histidine hydroxylase. Specifically demethylates 'Lys-4' (H3K4me) and 'Lys-36' (H3K36me) of histone H3, thereby playing a central role in histone code (By similarity). Mediates response to multiple stress stimuli, including heat shock and osmotic, oxidative, and ethanol stress"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"N(6),N(6)-dimethyl-L-lysyl(36)-[histone H3] + 2 2-oxoglutarate + 2 O2 = L-lysyl(36)-[histone H3] + 2 formaldehyde + 2 succinate + 2 CO2","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:42032"},{"database":"Rhea","id":"RHEA-COMP:9785"},{"database":"Rhea","id":"RHEA-COMP:9787"},{"database":"ChEBI","id":"CHEBI:15379"},{"database":"ChEBI","id":"CHEBI:16526"},{"database":"ChEBI","id":"CHEBI:16810"},{"database":"ChEBI","id":"CHEBI:16842"},{"database":"ChEBI","id":"CHEBI:29969"},{"database":"ChEBI","id":"CHEBI:30031"},{"database":"ChEBI","id":"CHEBI:61976"}],"ecNumber":"1.14.11.27"}},{"commentType":"COFACTOR","cofactors":[{"name":"Fe(2+)","evidences":[{"evidenceCode":"ECO:0000250"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:29033"}}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"Binds 1 Fe(2+) ion per subunit."}]}},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"Nucleus","id":"SL-0191"}}]},{"commentType":"ALTERNATIVE PRODUCTS","events":["Alternative splicing"],"isoforms":[{"name":{"value":"a"},"isoformIds":["O01658-1"],"isoformSequenceStatus":"Displayed"},{"name":{"value":"b"},"isoformIds":["O01658-2"],"sequenceIds":["VSP_053839","VSP_053840"],"isoformSequenceStatus":"Described"}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20057358"}],"value":"Attenuated response to heat shock stress and reduced survival after heat shock and oxidative stress"}],"commentType":"DISRUPTION PHENOTYPE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the ROX family. NO66 subfamily"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":748,"modifier":"EXACT"}},"description":"Bifunctional lysine-specific demethylase and histidyl-hydroxylase NO66","featureId":"PRO_0000390981"},{"type":"Domain","location":{"start":{"value":399,"modifier":"EXACT"},"end":{"value":543,"modifier":"EXACT"}},"description":"JmjC","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00538"}]},{"type":"Region","location":{"start":{"value":65,"modifier":"EXACT"},"end":{"value":135,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":160,"modifier":"EXACT"},"end":{"value":264,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":94,"modifier":"EXACT"},"end":{"value":110,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":124,"modifier":"EXACT"},"end":{"value":134,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":163,"modifier":"EXACT"},"end":{"value":193,"modifier":"EXACT"}},"description":"Acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":194,"modifier":"EXACT"},"end":{"value":203,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":207,"modifier":"EXACT"},"end":{"value":264,"modifier":"EXACT"}},"description":"Acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Binding site","location":{"start":{"value":442,"modifier":"EXACT"},"end":{"value":442,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:24875"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00538"}],"ligand":{"name":"Fe cation","id":"ChEBI:CHEBI:24875","note":"catalytic"}},{"type":"Binding site","location":{"start":{"value":444,"modifier":"EXACT"},"end":{"value":444,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:24875"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00538"}],"ligand":{"name":"Fe cation","id":"ChEBI:CHEBI:24875","note":"catalytic"}},{"type":"Binding site","location":{"start":{"value":509,"modifier":"EXACT"},"end":{"value":509,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:24875"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00538"}],"ligand":{"name":"Fe cation","id":"ChEBI:CHEBI:24875","note":"catalytic"}},{"type":"Alternative sequence","location":{"start":{"value":121,"modifier":"EXACT"},"end":{"value":125,"modifier":"EXACT"}},"description":"in isoform b","evidences":[{"evidenceCode":"ECO:0000305"}],"featureId":"VSP_053839","alternativeSequence":{"originalSequence":"HTALV","alternativeSequences":["RTAAS"]}},{"type":"Alternative sequence","location":{"start":{"value":126,"modifier":"EXACT"},"end":{"value":748,"modifier":"EXACT"}},"description":"in isoform b","evidences":[{"evidenceCode":"ECO:0000305"}],"featureId":"VSP_053840","alternativeSequence":{}}],"keywords":[{"id":"KW-0025","category":"Coding sequence diversity","name":"Alternative splicing"},{"id":"KW-0156","category":"Molecular function","name":"Chromatin regulator"},{"id":"KW-0223","category":"Molecular function","name":"Dioxygenase"},{"id":"KW-0408","category":"Ligand","name":"Iron"},{"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"id":"KW-0539","category":"Cellular component","name":"Nucleus"},{"id":"KW-0560","category":"Molecular function","name":"Oxidoreductase"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0678","category":"Molecular function","name":"Repressor"},{"id":"KW-0804","category":"Biological process","name":"Transcription"},{"id":"KW-0805","category":"Biological process","name":"Transcription regulation"}],"references":[{"referenceNumber":1,"citation":{"id":"9851916","citationType":"journal article","authoringGroup":["The C. elegans sequencing consortium"],"citationCrossReferences":[{"database":"PubMed","id":"9851916"},{"database":"DOI","id":"10.1126/science.282.5396.2012"}],"title":"Genome sequence of the nematode C. elegans: a platform for investigating biology.","publicationDate":"1998","journal":"Science","firstPage":"2012","lastPage":"2018","volume":"282"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]","ALTERNATIVE SPLICING"],"referenceComments":[{"value":"Bristol N2","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"20057358","citationType":"journal article","authors":["Kirienko N.V.","Fay D.S."],"citationCrossReferences":[{"database":"PubMed","id":"20057358"},{"database":"DOI","id":"10.1038/emboj.2009.387"}],"title":"SLR-2 and JMJC-1 regulate an evolutionarily conserved stress-response network.","publicationDate":"2010","journal":"EMBO J.","firstPage":"727","lastPage":"739","volume":"29"},"referencePositions":["FUNCTION","DISRUPTION PHENOTYPE"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"FO080999","properties":[{"key":"ProteinId","value":"CCD68363.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"FO080999","properties":[{"key":"ProteinId","value":"CCD68364.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"T15138","properties":[{"key":"EntryName","value":"T15138"}]},{"database":"RefSeq","id":"NP_001021644.1","properties":[{"key":"NucleotideSequenceId","value":"NM_001026473.7"}],"isoformId":"O01658-1"},{"database":"RefSeq","id":"NP_001021645.1","properties":[{"key":"NucleotideSequenceId","value":"NM_001026474.4"}],"isoformId":"O01658-2"},{"database":"AlphaFoldDB","id":"O01658","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"O01658","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"37407","properties":[{"key":"Interactions","value":"2"}]},{"database":"FunCoup","id":"O01658","properties":[{"key":"Number of interactors","value":"2469"}]},{"database":"STRING","id":"6239.T28F2.4a.1","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"6239-T28F2.4a.1","properties":[{"key":"Description","value":"-"}]},{"database":"PeptideAtlas","id":"O01658","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblMetazoa","id":"T28F2.4a.1","properties":[{"key":"ProteinId","value":"T28F2.4a.1"},{"key":"GeneId","value":"WBGene00020902"}]},{"database":"EnsemblMetazoa","id":"T28F2.4b.1","properties":[{"key":"ProteinId","value":"T28F2.4b.1"},{"key":"GeneId","value":"WBGene00020902"}]},{"database":"GeneID","id":"171932","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"cel:CELE_T28F2.4","properties":[{"key":"Description","value":"-"}]},{"database":"UCSC","id":"T28F2.4a","properties":[{"key":"OrganismName","value":"c. elegans"}],"isoformId":"O01658-1"},{"database":"AGR","id":"WB:WBGene00020902","properties":[{"key":"Description","value":"-"}]},{"database":"CTD","id":"171932","properties":[{"key":"Description","value":"-"}]},{"database":"WormBase","id":"T28F2.4a","properties":[{"key":"ProteinId","value":"CE28497"},{"key":"GeneId","value":"WBGene00020902"},{"key":"GeneName","value":"jmjc-1"}],"isoformId":"O01658-1"},{"database":"WormBase","id":"T28F2.4b","properties":[{"key":"ProteinId","value":"CE37050"},{"key":"GeneId","value":"WBGene00020902"},{"key":"GeneName","value":"jmjc-1"}],"isoformId":"O01658-2"},{"database":"eggNOG","id":"KOG3706","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"HOGENOM","id":"CLU_013645_4_0_1","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"O01658","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"PVFEGWI","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"425950at2759","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"O01658","properties":[{"key":"Description","value":"-"}]},{"database":"Reactome","id":"R-CEL-9629569","properties":[{"key":"PathwayName","value":"Protein hydroxylation"}]},{"database":"PRO","id":"PR:O01658","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000001940","properties":[{"key":"Component","value":"Chromosome I"}]},{"database":"Bgee","id":"WBGene00020902","properties":[{"key":"ExpressionPatterns","value":"Expressed in embryo and 3 other cell types or tissues"}]},{"database":"GO","id":"GO:0005730","properties":[{"key":"GoTerm","value":"C:nucleolus"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0005634","properties":[{"key":"GoTerm","value":"C:nucleus"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0016706","properties":[{"key":"GoTerm","value":"F:2-oxoglutarate-dependent dioxygenase activity"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0051864","properties":[{"key":"GoTerm","value":"F:histone H3K36 demethylase activity"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0140680","properties":[{"key":"GoTerm","value":"F:histone H3K36me/H3K36me2 demethylase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0032453","properties":[{"key":"GoTerm","value":"F:histone H3K4 demethylase activity"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0034647","properties":[{"key":"GoTerm","value":"F:histone H3K4me/H3K4me2/H3K4me3 demethylase activity"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0005506","properties":[{"key":"GoTerm","value":"F:iron ion binding"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0045892","properties":[{"key":"GoTerm","value":"P:negative regulation of DNA-templated transcription"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0045471","properties":[{"key":"GoTerm","value":"P:response to ethanol"},{"key":"GoEvidenceType","value":"IMP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"20057358"}]},{"database":"GO","id":"GO:0009408","properties":[{"key":"GoTerm","value":"P:response to heat"},{"key":"GoEvidenceType","value":"IMP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"20057358"}]},{"database":"GO","id":"GO:0006970","properties":[{"key":"GoTerm","value":"P:response to osmotic stress"},{"key":"GoEvidenceType","value":"IMP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"20057358"}]},{"database":"GO","id":"GO:0006979","properties":[{"key":"GoTerm","value":"P:response to oxidative stress"},{"key":"GoEvidenceType","value":"IMP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"20057358"}]},{"database":"FunFam","id":"2.60.120.650:FF:000013","properties":[{"key":"EntryName","value":"Ribosomal oxygenase 1"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.10.10.1500:FF:000001","properties":[{"key":"EntryName","value":"ribosomal oxygenase 1 isoform X1"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.90.930.40:FF:000001","properties":[{"key":"EntryName","value":"ribosomal oxygenase 1 isoform X1"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.90.930.40","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.60.120.650","properties":[{"key":"EntryName","value":"Cupin"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.10.1500","properties":[{"key":"EntryName","value":"JmjC domain-containing ribosomal oxygenase (ROX), dimer domain"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR003347","properties":[{"key":"EntryName","value":"JmjC_dom"}]},{"database":"InterPro","id":"IPR039994","properties":[{"key":"EntryName","value":"NO66-like"}]},{"database":"InterPro","id":"IPR049043","properties":[{"key":"EntryName","value":"WHD_RIOX1"}]},{"database":"PANTHER","id":"PTHR13096","properties":[{"key":"EntryName","value":"MINA53 MYC INDUCED NUCLEAR ANTIGEN"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR13096:SF8","properties":[{"key":"EntryName","value":"RIBOSOMAL OXYGENASE 1"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF08007","properties":[{"key":"EntryName","value":"JmjC_2"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF21233","properties":[{"key":"EntryName","value":"WHD_RIOX1"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF51197","properties":[{"key":"EntryName","value":"Clavaminate synthase-like"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51184","properties":[{"key":"EntryName","value":"JMJC"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MGKKKNSNKSAAAAPAVKHNDRWSSIELGEAKSAAVSHYKEPSKEPKFVHPAKLEKVKRIHDGLNIDRVLSHGPVPKQNGGTKRKHVEVTTQKLENKKPKVEVKKEDEKSKNKKMKNQNKHTALVQNETSTRSTYFVEEPDNENKVTLISNGREIAFKKTEVVESDDEQMIGLDSDEELEDEDETDIDEDEMMIDPKDIERYINFESVEDEEDMEDEEIEDEEFEDEEFEDEEEEADEQEEEEEDVSDEESVVSEMDADSDDEGFIAGKDREAHVISKDKFTRNAPAVDFDKFPFTDEDSVVTSSRAFGFMISPCDVQTFFDKFYQSNVLVVRRKQPTYFGNLFSTARLGELLEKNHLEYGRNINIAQYKNGVRTTLNGQGRAYPQIVKQHLHNMCSVQLVNPQTYDDRIWYLCEVIQEQFGCFVGANTYLTPAGSSGFAPHWDEIDAFLLQVEGRKYWRVWAPESAEEELPLESSDNFTEDDMKGREPVFEGWIEKGDMIYIPRGYIHQARTDSKVHSLHVTVSTGRQWSFANLMEKVVPEAIGVLTDTRHKLRRGLPTGLFDMGGVIDLDYSQEDHFVEKFKMVVDRHMSMLRNLVADQLLESSVDSLAKEFMKQALPPRLTEQEKKLSVLGSSTNLLGDDLVDFTARTKVRLIRRHTQRLLMESEDACFISHRINNSRLFEGRPEQIVEYPISGIDAYRVLSNSYPEWRTLYEIFSLRETKTKSRKENLAAIQLLFQIGVLLVKN","length":748,"molWeight":86026,"crc64":"A3E1587BAAB5E3AA","md5":"2AA28DB8A1ACBFEDD7EA89B5A1708B2C"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"COFACTOR":1,"SUBCELLULAR LOCATION":1,"ALTERNATIVE PRODUCTS":2,"DISRUPTION PHENOTYPE":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Domain":1,"Region":2,"Compositional bias":5,"Binding site":3,"Alternative sequence":2},"uniParcId":"UPI0000080619"}}