{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"K2PFJ6","uniProtkbId":"DARTG_THEA7","entryAudit":{"firstPublicDate":"2022-08-03","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2012-11-28","entryVersion":32,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Thermosipho africanus (strain H17ap60334)","taxonId":1161912,"lineage":["Bacteria","Thermotogati","Thermotogota","Thermotogae","Thermotogales","Fervidobacteriaceae","Thermosipho"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"DNA ADP-ribosyl transferase-DNA ADP-ribosyl glycohydrolase fusion protein"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"34408320"}],"value":"DarTG"}],"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O53604"}],"value":"2.4.2.-"},{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O53605"}],"value":"3.2.2.-"}]}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"darTG"},"orfNames":[{"evidences":[{"evidenceCode":"ECO:0000312","source":"EMBL","id":"EKF49129.1"}],"value":"H17ap60334_07413"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O53604"},{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O53605"}],"value":"A fusion protein of the toxic and antitoxin components of a hybrid type II/IV toxin-antitoxin (TA) system. The N-terminal domain ADP-ribosylates ssDNA on a thymidine residue, while the C-terminal domain removes the modification, neutralizing the toxic effect"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"an N-(ADP-alpha-D-ribosyl)-thymidine in DNA + H2O = a thymidine in DNA + ADP-D-ribose","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:71655"},{"database":"Rhea","id":"RHEA-COMP:13556"},{"database":"Rhea","id":"RHEA-COMP:18051"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:57967"},{"database":"ChEBI","id":"CHEBI:137386"},{"database":"ChEBI","id":"CHEBI:191199"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:71656"},"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O53605"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a thymidine in DNA + NAD(+) = an N-(ADP-alpha-D-ribosyl)-thymidine in DNA + nicotinamide + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:71651"},{"database":"Rhea","id":"RHEA-COMP:13556"},{"database":"Rhea","id":"RHEA-COMP:18051"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:17154"},{"database":"ChEBI","id":"CHEBI:57540"},{"database":"ChEBI","id":"CHEBI:137386"},{"database":"ChEBI","id":"CHEBI:191199"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01362"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:71652"},"evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01362"}]}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"A0A0B0SG80"}],"value":"The NAD(+)-binding element stabilizes the ADP-ribosylating turn-turn (ARTT) loop which confers substrate specificity; both domains contribute to ssDNA-binding"}],"commentType":"DOMAIN"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"In the N-terminal section; belongs to the DarT ADP-ribosyltransferase family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"In the C-terminal section; belongs to the DarG ADP-ribosyl glycohydrolase family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"This is a fusion of 2 opposing activities; its in vivo function is unclear"}],"commentType":"CAUTION"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":388,"modifier":"EXACT"}},"description":"DNA ADP-ribosyl transferase-DNA ADP-ribosyl glycohydrolase fusion protein","featureId":"PRO_0000456053"},{"type":"Domain","location":{"start":{"value":6,"modifier":"EXACT"},"end":{"value":197,"modifier":"EXACT"}},"description":"DarT","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01362"}]},{"type":"Domain","location":{"start":{"value":196,"modifier":"EXACT"},"end":{"value":376,"modifier":"EXACT"}},"description":"Macro","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00490"}]},{"type":"Region","location":{"start":{"value":34,"modifier":"EXACT"},"end":{"value":52,"modifier":"EXACT"}},"description":"NAD(+)-binding element","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34408320"}]},{"type":"Region","location":{"start":{"value":107,"modifier":"EXACT"},"end":{"value":152,"modifier":"EXACT"}},"description":"ADP-ribosylating turn-turn loop","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34408320"}]},{"type":"Active site","location":{"start":{"value":50,"modifier":"EXACT"},"end":{"value":50,"modifier":"EXACT"}},"description":"Proton acceptor","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01362"}]},{"type":"Active site","location":{"start":{"value":152,"modifier":"EXACT"},"end":{"value":152,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01362"}]},{"type":"Binding site","location":{"start":{"value":10,"modifier":"EXACT"},"end":{"value":12,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57540"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01362"}],"ligand":{"name":"NAD(+)","id":"ChEBI:CHEBI:57540"}},{"type":"Binding site","location":{"start":{"value":50,"modifier":"EXACT"},"end":{"value":50,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57540"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01362"}],"ligand":{"name":"NAD(+)","id":"ChEBI:CHEBI:57540"}},{"type":"Binding site","location":{"start":{"value":215,"modifier":"EXACT"},"end":{"value":216,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57967"}],"evidences":[{"evidenceCode":"ECO:0007744","source":"PDB","id":"7OMU"}],"ligand":{"name":"ADP-D-ribose","id":"ChEBI:CHEBI:57967"}},{"type":"Binding site","location":{"start":{"value":227,"modifier":"EXACT"},"end":{"value":229,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57967"}],"evidences":[{"evidenceCode":"ECO:0007744","source":"PDB","id":"7OMU"}],"ligand":{"name":"ADP-D-ribose","id":"ChEBI:CHEBI:57967"}},{"type":"Binding site","location":{"start":{"value":301,"modifier":"EXACT"},"end":{"value":301,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57967"}],"evidences":[{"evidenceCode":"ECO:0007744","source":"PDB","id":"7OMU"}],"ligand":{"name":"ADP-D-ribose","id":"ChEBI:CHEBI:57967"}},{"type":"Binding site","location":{"start":{"value":339,"modifier":"EXACT"},"end":{"value":343,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57967"}],"evidences":[{"evidenceCode":"ECO:0007744","source":"PDB","id":"7OMU"}],"ligand":{"name":"ADP-D-ribose","id":"ChEBI:CHEBI:57967"}},{"type":"Binding site","location":{"start":{"value":371,"modifier":"EXACT"},"end":{"value":372,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57967"}],"evidences":[{"evidenceCode":"ECO:0007744","source":"PDB","id":"7OMU"}],"ligand":{"name":"ADP-D-ribose","id":"ChEBI:CHEBI:57967"}},{"type":"Beta strand","location":{"start":{"value":7,"modifier":"EXACT"},"end":{"value":13,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7OMU"}]},{"type":"Helix","location":{"start":{"value":14,"modifier":"EXACT"},"end":{"value":16,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7OMU"}]},{"type":"Helix","location":{"start":{"value":17,"modifier":"EXACT"},"end":{"value":23,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7OMU"}]},{"type":"Helix","location":{"start":{"value":28,"modifier":"EXACT"},"end":{"value":33,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7OMU"}]},{"type":"Helix","location":{"start":{"value":44,"modifier":"EXACT"},"end":{"value":52,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7OMU"}]},{"type":"Turn","location":{"start":{"value":56,"modifier":"EXACT"},"end":{"value":58,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7OMU"}]},{"type":"Helix","location":{"start":{"value":61,"modifier":"EXACT"},"end":{"value":63,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7OMU"}]},{"type":"Beta strand","location":{"start":{"value":64,"modifier":"EXACT"},"end":{"value":69,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7OMU"}]},{"type":"Beta strand","location":{"start":{"value":71,"modifier":"EXACT"},"end":{"value":73,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7OMU"}]},{"type":"Helix","location":{"start":{"value":74,"modifier":"EXACT"},"end":{"value":81,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7OMU"}]},{"type":"Helix","location":{"start":{"value":86,"modifier":"EXACT"},"end":{"value":88,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7OMU"}]},{"type":"Beta strand","location":{"start":{"value":89,"modifier":"EXACT"},"end":{"value":95,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7OMU"}]},{"type":"Helix","location":{"start":{"value":96,"modifier":"EXACT"},"end":{"value":100,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7OMU"}]},{"type":"Beta strand","location":{"start":{"value":105,"modifier":"EXACT"},"end":{"value":109,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7OMU"}]},{"type":"Beta strand","location":{"start":{"value":111,"modifier":"EXACT"},"end":{"value":115,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7OMU"}]},{"type":"Beta strand","location":{"start":{"value":118,"modifier":"EXACT"},"end":{"value":120,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7OMU"}]},{"type":"Helix","location":{"start":{"value":121,"modifier":"EXACT"},"end":{"value":123,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7OMU"}]},{"type":"Helix","location":{"start":{"value":127,"modifier":"EXACT"},"end":{"value":130,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7OMU"}]},{"type":"Helix","location":{"start":{"value":131,"modifier":"EXACT"},"end":{"value":135,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7OMU"}]},{"type":"Helix","location":{"start":{"value":145,"modifier":"EXACT"},"end":{"value":148,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7OMU"}]},{"type":"Beta strand","location":{"start":{"value":152,"modifier":"EXACT"},"end":{"value":159,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7OMU"}]},{"type":"Helix","location":{"start":{"value":161,"modifier":"EXACT"},"end":{"value":163,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7OMU"}]},{"type":"Beta strand","location":{"start":{"value":164,"modifier":"EXACT"},"end":{"value":171,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7OMU"}]},{"type":"Helix","location":{"start":{"value":172,"modifier":"EXACT"},"end":{"value":182,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7OMU"}]},{"type":"Beta strand","location":{"start":{"value":189,"modifier":"EXACT"},"end":{"value":191,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7OMU"}]},{"type":"Helix","location":{"start":{"value":193,"modifier":"EXACT"},"end":{"value":196,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7OMU"}]},{"type":"Beta strand","location":{"start":{"value":200,"modifier":"EXACT"},"end":{"value":214,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7OMU"}]},{"type":"Helix","location":{"start":{"value":216,"modifier":"EXACT"},"end":{"value":218,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7OMU"}]},{"type":"Beta strand","location":{"start":{"value":222,"modifier":"EXACT"},"end":{"value":228,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7OMU"}]},{"type":"Beta strand","location":{"start":{"value":230,"modifier":"EXACT"},"end":{"value":232,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7OMU"}]},{"type":"Helix","location":{"start":{"value":238,"modifier":"EXACT"},"end":{"value":245,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7OMU"}]},{"type":"Helix","location":{"start":{"value":247,"modifier":"EXACT"},"end":{"value":257,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7OMU"}]},{"type":"Turn","location":{"start":{"value":258,"modifier":"EXACT"},"end":{"value":260,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7OMU"}]},{"type":"Beta strand","location":{"start":{"value":268,"modifier":"EXACT"},"end":{"value":270,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7OMU"}]},{"type":"Helix","location":{"start":{"value":276,"modifier":"EXACT"},"end":{"value":280,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7OMU"}]},{"type":"Beta strand","location":{"start":{"value":295,"modifier":"EXACT"},"end":{"value":300,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7OMU"}]},{"type":"Beta strand","location":{"start":{"value":303,"modifier":"EXACT"},"end":{"value":307,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7OMU"}]},{"type":"Helix","location":{"start":{"value":311,"modifier":"EXACT"},"end":{"value":328,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7OMU"}]},{"type":"Beta strand","location":{"start":{"value":332,"modifier":"EXACT"},"end":{"value":336,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7OMU"}]},{"type":"Beta strand","location":{"start":{"value":340,"modifier":"EXACT"},"end":{"value":343,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7OMU"}]},{"type":"Helix","location":{"start":{"value":347,"modifier":"EXACT"},"end":{"value":358,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7OMU"}]},{"type":"Beta strand","location":{"start":{"value":365,"modifier":"EXACT"},"end":{"value":368,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7OMU"}]},{"type":"Beta strand","location":{"start":{"value":371,"modifier":"EXACT"},"end":{"value":373,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7OMU"}]},{"type":"Helix","location":{"start":{"value":374,"modifier":"EXACT"},"end":{"value":376,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7OMU"}]},{"type":"Helix","location":{"start":{"value":377,"modifier":"EXACT"},"end":{"value":380,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7OMU"}]},{"type":"Helix","location":{"start":{"value":382,"modifier":"EXACT"},"end":{"value":385,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"7OMU"}]}],"keywords":[{"id":"KW-0002","category":"Technical term","name":"3D-structure"},{"id":"KW-0238","category":"Molecular function","name":"DNA-binding"},{"id":"KW-0328","category":"Molecular function","name":"Glycosyltransferase"},{"id":"KW-0378","category":"Molecular function","name":"Hydrolase"},{"id":"KW-0548","category":"Molecular function","name":"Nucleotidyltransferase"},{"id":"KW-1277","category":"Biological process","name":"Toxin-antitoxin system"},{"id":"KW-0808","category":"Molecular function","name":"Transferase"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-5HMRCPK1AJ9TK","citationType":"submission","authors":["Nesbo C.L.","Swithers K.","Dlutek M.","Marquis P.","Doolittle F.W."],"title":"Genome sequence of Thermosipho africanus H17ap60334.","publicationDate":"MAR-2012","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000312","source":"EMBL","id":"EKF49129.1"}],"value":"H17ap60334","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000312","source":"EMBL","id":"EKF49129.1"}]},{"referenceNumber":2,"citation":{"id":"34408320","citationType":"journal article","authors":["Schuller M.","Butler R.E.","Ariza A.","Tromans-Coia C.","Jankevicius G.","Claridge T.D.W.","Kendall S.L.","Goh S.","Stewart G.R.","Ahel I."],"citationCrossReferences":[{"database":"PubMed","id":"34408320"},{"database":"DOI","id":"10.1038/s41586-021-03825-4"}],"title":"Molecular basis for DarT ADP-ribosylation of a DNA base.","publicationDate":"2021","journal":"Nature","firstPage":"597","lastPage":"602","volume":"596"},"referencePositions":["X-RAY CRYSTALLOGRAPHY (2.96 ANGSTROMS) IN COMPLEX WITH ADP-RIBOSE"],"evidences":[{"evidenceCode":"ECO:0007744","source":"PDB","id":"7OMU"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AJIP01000032","properties":[{"key":"ProteinId","value":"EKF49129.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_004103096.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_AJIP01000032.1"}]},{"database":"PDB","id":"7OMU","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.96 A"},{"key":"Chains","value":"AAA/BBB=1-388"}]},{"database":"PDBsum","id":"7OMU","properties":[{"key":"Description","value":"-"}]},{"database":"AlphaFoldDB","id":"K2PFJ6","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"K2PFJ6","properties":[{"key":"Description","value":"-"}]},{"database":"PATRIC","id":"fig|1161912.4.peg.1460","properties":[{"key":"GeneDesignation","value":"-"}]},{"database":"GO","id":"GO:0140291","properties":[{"key":"GoTerm","value":"P:peptidyl-glutamate ADP-deribosylation"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"CDD","id":"cd02901","properties":[{"key":"EntryName","value":"Macro_Poa1p-like"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.220.10","properties":[{"key":"EntryName","value":"Leucine Aminopeptidase, subunit E, domain 1"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR050892","properties":[{"key":"EntryName","value":"ADP-ribose_metab_enzymes"}]},{"database":"InterPro","id":"IPR029494","properties":[{"key":"EntryName","value":"DarT"}]},{"database":"InterPro","id":"IPR002589","properties":[{"key":"EntryName","value":"Macro_dom"}]},{"database":"InterPro","id":"IPR043472","properties":[{"key":"EntryName","value":"Macro_dom-like"}]},{"database":"PANTHER","id":"PTHR12521:SF0","properties":[{"key":"EntryName","value":"ADP-RIBOSE GLYCOHYDROLASE OARD1"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR12521","properties":[{"key":"EntryName","value":"PROTEIN C6ORF130"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF14487","properties":[{"key":"EntryName","value":"DarT"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01661","properties":[{"key":"EntryName","value":"Macro"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF52949","properties":[{"key":"EntryName","value":"Macro domain-like"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS52018","properties":[{"key":"EntryName","value":"DART"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51154","properties":[{"key":"EntryName","value":"MACRO"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MFPRFRELYYITHIDNVPSILEKGILSHAEIERQSINCKKVYDNSIVLKRKSRLLADNRSLWEFANLYFQPRNPMLYRLLVQGLKPKDLAIVAVKWTIMKRDDILITDGNAASSETQIYRKSEIKNIKNIISVKDMEYWREEDGSKRKIMAECLVPQCVDPRYISAIYVSDHEVASNLKKAINNRNIPVIPDPTFFFLPNREIKLTQNLSLVEGDMFFSRMQTLTVSVNTVGVMGKGLASRVKYQFPDVYVVFQDACKKKELEFGKPYLYKRESSLDAFLAEDGEKLSDLNHQTWFLLFPTKRHWKNMSEIKGIESGLRWIVENYKKEGIKSLAVPALGCGLGGLEWSIVGPLMCRYLTKLEIPVQIYLPLEKRIPDVQLSPKFLLDS","length":388,"molWeight":44883,"crc64":"AB552526040312B8","md5":"6D029C2B2D992FD24780E97C5851612A"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":2,"DOMAIN":1,"SIMILARITY":2,"CAUTION":1},"countByFeatureType":{"Chain":1,"Domain":2,"Region":2,"Active site":2,"Binding site":7,"Beta strand":20,"Helix":24,"Turn":2},"uniParcId":"UPI00028C67CA"}}