{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"J9VKY2","uniProtkbId":"PRL65_CRYN9","entryAudit":{"firstPublicDate":"2024-11-27","lastAnnotationUpdateDate":"2026-06-10","lastSequenceUpdateDate":"2012-11-28","entryVersion":45,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Cryptococcus neoformans (strain H99 / ATCC 208821 / CBS 10515 / FGSC 9487)","commonName":"Cryptococcus neoformans var. grubii serotype A","taxonId":235443,"evidences":[{"evidenceCode":"ECO:0000312","source":"Proteomes","id":"UP000010091"}],"lineage":["Eukaryota","Fungi","Dikarya","Basidiomycota","Agaricomycotina","Tremellomycetes","Tremellales","Cryptococcaceae","Cryptococcus","Cryptococcus neoformans species complex"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Putative primary metabolism protein HVA1"}},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"27583447"}],"value":"Hypervirulence-associated protein 1"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"27583447"}],"value":"HVA1"},"orfNames":[{"evidences":[{"evidenceCode":"ECO:0000312","source":"EMBL","id":"AFR94101.1"}],"value":"CNAG_02591"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27583447"}],"value":"May play a role in primary metabolism"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27583447"}],"value":"Repressed in models of infection"}],"commentType":"INDUCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27583447"}],"value":"Leads to abnormal metabolite levels including decreased levels of 2-ketoglutarate and increased levels of phosphoenolpyruvate (PubMed:27583447). When in the presence of NADPH, increases virulence in a macrophage infection model (PubMed:27583447)"}],"commentType":"DISRUPTION PHENOTYPE"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":75,"modifier":"EXACT"}},"description":"Putative primary metabolism protein HVA1","featureId":"PRO_0000461183"},{"type":"Region","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":24,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":40,"modifier":"EXACT"},"end":{"value":75,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":13,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Beta strand","location":{"start":{"value":16,"modifier":"EXACT"},"end":{"value":21,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4P5N"}]},{"type":"Beta strand","location":{"start":{"value":24,"modifier":"EXACT"},"end":{"value":34,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4P5N"}]},{"type":"Helix","location":{"start":{"value":37,"modifier":"EXACT"},"end":{"value":43,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4P5N"}]},{"type":"Beta strand","location":{"start":{"value":50,"modifier":"EXACT"},"end":{"value":54,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4P5N"}]},{"type":"Beta strand","location":{"start":{"value":60,"modifier":"EXACT"},"end":{"value":63,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4P5N"}]},{"type":"Helix","location":{"start":{"value":65,"modifier":"EXACT"},"end":{"value":67,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4P5N"}]},{"type":"Beta strand","location":{"start":{"value":68,"modifier":"EXACT"},"end":{"value":70,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4P5N"}]},{"type":"Helix","location":{"start":{"value":71,"modifier":"EXACT"},"end":{"value":73,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4P5N"}]}],"keywords":[{"id":"KW-0002","category":"Technical term","name":"3D-structure"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"}],"references":[{"referenceNumber":1,"citation":{"id":"24743168","citationType":"journal article","authors":["Janbon G.","Ormerod K.L.","Paulet D.","Byrnes E.J. III","Yadav V.","Chatterjee G.","Mullapudi N.","Hon C.-C.","Billmyre R.B.","Brunel F.","Bahn Y.-S.","Chen W.","Chen Y.","Chow E.W.L.","Coppee J.-Y.","Floyd-Averette A.","Gaillardin C.","Gerik K.J.","Goldberg J.","Gonzalez-Hilarion S.","Gujja S.","Hamlin J.L.","Hsueh Y.-P.","Ianiri G.","Jones S.","Kodira C.D.","Kozubowski L.","Lam W.","Marra M.","Mesner L.D.","Mieczkowski P.A.","Moyrand F.","Nielsen K.","Proux C.","Rossignol T.","Schein J.E.","Sun S.","Wollschlaeger C.","Wood I.A.","Zeng Q.","Neuveglise C.","Newlon C.S.","Perfect J.R.","Lodge J.K.","Idnurm A.","Stajich J.E.","Kronstad J.W.","Sanyal K.","Heitman J.","Fraser J.A.","Cuomo C.A.","Dietrich F.S."],"citationCrossReferences":[{"database":"PubMed","id":"24743168"},{"database":"DOI","id":"10.1371/journal.pgen.1004261"}],"title":"Analysis of the genome and transcriptome of Cryptococcus neoformans var. grubii reveals complex RNA expression and microevolution leading to virulence attenuation.","publicationDate":"2014","journal":"PLoS Genet.","firstPage":"E1004261","lastPage":"E1004261","volume":"10"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"H99 / ATCC 208821 / CBS 10515 / FGSC 9487","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000312","source":"Proteomes","id":"UP000010091"}]},{"referenceNumber":2,"citation":{"id":"27583447","citationType":"journal article","authors":["McClelland E.E.","Ramagopal U.A.","Rivera J.","Cox J.","Nakouzi A.","Prabu M.M.","Almo S.C.","Casadevall A."],"citationCrossReferences":[{"database":"PubMed","id":"27583447"},{"database":"DOI","id":"10.1371/journal.ppat.1005849"}],"title":"A Small Protein Associated with Fungal Energy Metabolism Affects the Virulence of Cryptococcus neoformans in Mammals.","publicationDate":"2016","journal":"PLoS Pathog.","firstPage":"e1005849","lastPage":"e1005849","volume":"12"},"referencePositions":["X-RAY CRYSTALLOGRAPHY (1.49 ANGSTROMS)","FUNCTION","INDUCTION","DISRUPTION PHENOTYPE"],"evidences":[{"evidenceCode":"ECO:0007744","source":"PDB","id":"4P5N"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"CP003822","properties":[{"key":"ProteinId","value":"AFR94101.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"XP_012048039.1","properties":[{"key":"NucleotideSequenceId","value":"XM_012192649.1"}]},{"database":"PDB","id":"4P5N","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.49 A"},{"key":"Chains","value":"A/B=1-75"}]},{"database":"PDBsum","id":"4P5N","properties":[{"key":"Description","value":"-"}]},{"database":"AlphaFoldDB","id":"J9VKY2","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"J9VKY2","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"23886202","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"cng:CNAG_02591","properties":[{"key":"Description","value":"-"}]},{"database":"VEuPathDB","id":"FungiDB:CNAG_02591","properties":[{"key":"Description","value":"-"}]},{"database":"HOGENOM","id":"CLU_177181_0_1_1","properties":[{"key":"Description","value":"-"}]},{"database":"OrthoDB","id":"1489at5206","properties":[{"key":"Description","value":"-"}]},{"database":"EvolutionaryTrace","id":"J9VKY2","properties":[{"key":"Description","value":"-"}]},{"database":"PHI-base","id":"PHI:6605","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000010091","properties":[{"key":"Component","value":"Chromosome 3"}]},{"database":"Gene3D","id":"2.30.30.1060","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR021331","properties":[{"key":"EntryName","value":"Hva1_TUDOR"}]},{"database":"Pfam","id":"PF11160","properties":[{"key":"EntryName","value":"Hva1_TUDOR"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MSVQDKQGQNINVGDTVYTPYRGGKHEGQVADIVTTKEEAAEKGVKNPPKVLFTDQNNKDVAHNPGTLTDLDKQK","length":75,"molWeight":8208,"crc64":"1F72CFC8D4EF1EEF","md5":"6B3C7D130A271C019673FCCA8615CA31"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"INDUCTION":1,"DISRUPTION PHENOTYPE":1},"countByFeatureType":{"Chain":1,"Region":2,"Compositional bias":1,"Beta strand":5,"Helix":3},"uniParcId":"UPI00027E3807"}}