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Together with SPO11, mediates DNA cleavage that forms the double-strand breaks (DSB) that initiate meiotic recombination (PubMed:26917764, PubMed:39972129, PubMed:39972130). The complex promotes relaxation of negative and positive supercoiled DNA and DNA decatenation through cleavage and ligation cycles (PubMed:26917764, PubMed:39972129, PubMed:39972130). Within the complex, TOP6BL may sense specific DNA architectures, such as bendable and underwound DNA for cleavage by SPO11 (PubMed:38966985)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"26917764"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"36396648"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"38966985"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"39972129"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"39972130"}],"value":"Heterotetramer of two SPO11 and two TOP6BL chains (PubMed:26917764, PubMed:38966985, PubMed:39972129, PubMed:39972130). Interacts with REC114; promoting the activity of the SPO11-TOP6BL heterotetramer and formation of meiotic DNA double-strand breaks (PubMed:36396648)"}],"commentType":"SUBUNIT"},{"commentType":"INTERACTION","interactions":[{"interactantOne":{"uniProtKBAccession":"J3QMY9","intActId":"EBI-16200997"},"interactantTwo":{"uniProtKBAccession":"Q9WTK8-1","geneName":"Spo11","intActId":"EBI-16201014"},"numberOfExperiments":6,"organismDiffer":false}]},{"commentType":"SUBCELLULAR LOCATION","note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"39972129"}],"value":"Localizes to meiotic chromosomes together with SPO11"}]},"subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"39972129"}],"value":"Chromosome","id":"SL-0468"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"39972129"}],"value":"Nucleus","id":"SL-0191"}}]},{"commentType":"ALTERNATIVE PRODUCTS","events":["Alternative splicing"],"isoforms":[{"name":{"value":"1"},"isoformIds":["J3QMY9-1"],"isoformSequenceStatus":"Displayed"},{"name":{"value":"2"},"isoformIds":["J3QMY9-2"],"sequenceIds":["VSP_058336","VSP_058337"],"isoformSequenceStatus":"Described"}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"26917764"}],"value":"In males, expressed in testis but not in other tissues (PubMed:26917764). Expressed in testis from 8 days postpartum (dpp) onward, with a peak of expression around 12 to 14 dpp, at the beginning of meiotic prophase (PubMed:26917764). In females, expressed in embryonic ovaries when oocytes proceed through meiotic prophase (PubMed:26917764)"}],"commentType":"TISSUE SPECIFICITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"26917764"}],"value":"Despite a weak sequence similarity, retains most of the structural features of the ancestral archaeal Top6B subunit (AC O05207), including the transducer domain that interacts with the SPO11 subunit and the ATP-binding fold, also named GHKL fold"}],"commentType":"DOMAIN"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"26917764"}],"value":"Male mice develop normally and reach adulthood but have smaller testes. Spermatogonia are present at the periphery of tubules but are strongly depleted and spermatids are absent, due to defects during meiotic prophase"}],"commentType":"DISRUPTION PHENOTYPE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the TOP6B-like family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":579,"modifier":"EXACT"}},"description":"Type 2 DNA topoisomerase 6 subunit B-like","featureId":"PRO_0000436277"},{"type":"Region","location":{"start":{"value":480,"modifier":"EXACT"},"end":{"value":554,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":501,"modifier":"EXACT"},"end":{"value":528,"modifier":"EXACT"}},"description":"Basic and acidic 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