{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"I3RB26","uniProtkbId":"CAS6_HALMT","entryAudit":{"firstPublicDate":"2015-03-04","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2012-09-05","entryVersion":52,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Haloferax mediterranei (strain ATCC 33500 / DSM 1411 / JCM 8866 / NBRC 14739 / NCIMB 2177 / R-4)","commonName":"Halobacterium mediterranei","taxonId":523841,"lineage":["Archaea","Methanobacteriati","Methanobacteriota","Stenosarchaea group","Halobacteria","Halobacteriales","Haloferacaceae","Haloferax"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"value":"CRISPR-associated endoribonuclease Cas6"},"ecNumbers":[{"value":"3.1.-.-"}]}},"genes":[{"geneName":{"value":"cas6"},"orderedLocusNames":[{"value":"HFX_6314"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23243301"}],"value":"CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). This protein probably processes pre-crRNA into individual crRNA units, and also plays a role in generation or stability of the CRISPR leader-repeat transcript. Cleaves an endogenous (non-invading) RNA; cuts a specific site within a sequence nearly identical to the cognate CRISPR repeat sequence in the 5' UTR of the transcript of provirus gene phiH, found in provirus 1. This digestion might be responsible for inhibiting (defective) provirus 1 excision"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23243301"}],"value":"Does not produce mature length crRNA; precursor is non-specifically degraded. No accumulation of the CRISPR leader-repeat transcript. Allows excision of defective provirus 1 from its chromosomal locus (detected as restoration of the attachment site attA)"}],"commentType":"DISRUPTION PHENOTYPE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"23243301"}],"value":"There are 6 CRISPR RNA loci in this organism and a single cas gene locus. A CRISPR-Cas type I-B system"}],"commentType":"MISCELLANEOUS"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the CRISPR-associated protein Cas6/Cse3/CasE family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":270,"modifier":"EXACT"}},"description":"CRISPR-associated endoribonuclease Cas6","featureId":"PRO_0000432152"},{"type":"Active site","location":{"start":{"value":41,"modifier":"EXACT"},"end":{"value":41,"modifier":"EXACT"}},"description":"Proton donor","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"D4GQN4"}]}],"geneLocations":[{"value":"pHM500","geneEncodingType":"Plasmid"}],"keywords":[{"id":"KW-0051","category":"Biological process","name":"Antiviral defense"},{"id":"KW-0255","category":"Molecular function","name":"Endonuclease"},{"id":"KW-0378","category":"Molecular function","name":"Hydrolase"},{"id":"KW-0540","category":"Molecular function","name":"Nuclease"},{"id":"KW-0614","category":"Technical term","name":"Plasmid"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0694","category":"Molecular function","name":"RNA-binding"}],"references":[{"referenceNumber":1,"citation":{"id":"22843593","citationType":"journal article","authors":["Han J.","Zhang F.","Hou J.","Liu X.","Li M.","Liu H.","Cai L.","Zhang B.","Chen Y.","Zhou J.","Hu S.","Xiang H."],"citationCrossReferences":[{"database":"PubMed","id":"22843593"},{"database":"DOI","id":"10.1128/jb.00880-12"}],"title":"Complete genome sequence of the metabolically versatile halophilic archaeon Haloferax mediterranei, a poly(3-hydroxybutyrate-co-3-hydroxyvalerate) producer.","publicationDate":"2012","journal":"J. Bacteriol.","firstPage":"4463","lastPage":"4464","volume":"194"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"ATCC 33500 / DSM 1411 / JCM 8866 / NBRC 14739 / NCIMB 2177 / R-4","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"23243301","citationType":"journal article","authors":["Li M.","Liu H.","Han J.","Liu J.","Wang R.","Zhao D.","Zhou J.","Xiang H."],"citationCrossReferences":[{"database":"PubMed","id":"23243301"},{"database":"DOI","id":"10.1128/jb.01688-12"}],"title":"Characterization of CRISPR RNA biogenesis and Cas6 cleavage-mediated inhibition of a provirus in the haloarchaeon Haloferax mediterranei.","publicationDate":"2013","journal":"J. Bacteriol.","firstPage":"867","lastPage":"875","volume":"195"},"referencePositions":["FUNCTION","DISRUPTION PHENOTYPE"],"referenceComments":[{"value":"ATCC 33500 / DSM 1411 / JCM 8866 / NBRC 14739 / NCIMB 2177 / R-4","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"CP001871","properties":[{"key":"ProteinId","value":"AFK21436.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_014732817.1","properties":[{"key":"NucleotideSequenceId","value":"NC_017944.1"}]},{"database":"AlphaFoldDB","id":"I3RB26","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"I3RB26","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"40158021","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"hme:HFX_6314","properties":[{"key":"Description","value":"-"}]},{"database":"HOGENOM","id":"CLU_089858_1_0_2","properties":[{"key":"Description","value":"-"}]},{"database":"OrthoDB","id":"43942at2157","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000006469","properties":[{"key":"Component","value":"Plasmid pHM500"}]},{"database":"GO","id":"GO:0016788","properties":[{"key":"GoTerm","value":"F:hydrolase activity, acting on ester bonds"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"CDD","id":"cd21140","properties":[{"key":"EntryName","value":"Cas6_I-like"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.70.1890","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.70.1900","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR049435","properties":[{"key":"EntryName","value":"Cas_Cas6_C"}]},{"database":"InterPro","id":"IPR010156","properties":[{"key":"EntryName","value":"CRISPR-assoc_prot_Cas6"}]},{"database":"InterPro","id":"IPR045747","properties":[{"key":"EntryName","value":"CRISPR-assoc_prot_Cas6_N_sf"}]},{"database":"NCBIfam","id":"TIGR01877","properties":[{"key":"EntryName","value":"cas_cas6"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR36984","properties":[{"key":"EntryName","value":"CRISPR-ASSOCIATED ENDORIBONUCLEASE CAS6 1"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR36984:SF1","properties":[{"key":"EntryName","value":"CRISPR-ASSOCIATED ENDORIBONUCLEASE CAS6 1"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01881","properties":[{"key":"EntryName","value":"Cas_Cas6_C"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MRLMVHLEAQRDATYQTAYHHKLRGRVWRALEGSKFDSEHDNGHPLGLAFSNIFPWGKIQEGDRRSLLFASPREDLLATIARGLQRNRDFNVGEMSFEVDDLSQLTVDVGEPGTRGVIETATGVVVRLTPEHREQYGIEAEHDAPTYWRPEHTIKPFKDAIRANLQHKHDRFAHEYQDGPTDVDGELFEGYDLLKTYALPVTVTTGTTLDVVLSKWRFDYRVRNDAHRHHLNLALDAGIGGRNGLGFGFSNIVEKTKPGESELERADAFA","length":270,"molWeight":30616,"crc64":"4DEED8441FF75CE4","md5":"CB91A44A6E87987823B96B21A8A3B5B9"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"DISRUPTION PHENOTYPE":1,"MISCELLANEOUS":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Active site":1},"uniParcId":"UPI000260536F"}}