{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"H8EZH5","uniProtkbId":"RPFB_MYCTE","entryAudit":{"firstPublicDate":"2013-02-06","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2012-05-16","entryVersion":56,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Mycobacterium tuberculosis (strain ATCC 35801 / TMC 107 / Erdman)","taxonId":652616,"lineage":["Bacteria","Bacillati","Actinomycetota","Actinomycetes","Mycobacteriales","Mycobacteriaceae","Mycobacterium","Mycobacterium tuberculosis complex"]},"proteinExistence":"2: Evidence at transcript level","proteinDescription":{"recommendedName":{"fullName":{"value":"Resuscitation-promoting factor RpfB"},"ecNumbers":[{"value":"3.-.-.-"}]},"flag":"Precursor"},"genes":[{"geneName":{"value":"rpfB"},"orderedLocusNames":[{"value":"ERDMAN_1122"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"Factor that stimulates resuscitation of dormant cells. Has peptidoglycan (PG) hydrolytic activity. Active in the pM concentration range. Has little to no effect on actively-growing cells. PG fragments could either directly activate the resuscitation pathway of dormant bacteria or serve as a substrate for endogenous Rpf, resulting in low molecular weight products with resuscitation activity (By similarity). Plays a role in reactivating bacteria from chronic tuberculosis (TB) in mice"}],"commentType":"FUNCTION"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00303"}],"value":"Cell membrane","id":"SL-0039"},"topology":{"evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00303"}],"value":"Lipid-anchor","id":"SL-9901"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16622237"}],"value":"In infected C57BL/6 mice levels remains constant following reactivation of TB (induced by the nitric oxide synthase inhibitor aminoguanidine) for 4 weeks then decreases over 10-fold until at least 11 weeks"}],"commentType":"INDUCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16622237"}],"value":"No difference in chronic C57BL/6 mouse infection, but infected mice show delayed reactivation of TB (induced by aminoguanidine, a nitric oxide synthase inhibitor) compared to mice infected with wild-type bacteria. Bacterial loads in lung, liver, and spleen are similar during chronic infection, but increase more slowly in the disrupted mutant after reactivation. Pulmonary B-cell responses are reduced in mice infected with the disrupted strain at advanced stages of TB reactivation. While disease is delayed, it does eventually occur, suggesting other the Rpfs compensate"}],"commentType":"DISRUPTION PHENOTYPE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the transglycosylase family. Rpf subfamily"}],"commentType":"SIMILARITY"}],"features":[{"type":"Signal","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":23,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00303"}]},{"type":"Chain","location":{"start":{"value":24,"modifier":"EXACT"},"end":{"value":362,"modifier":"EXACT"}},"description":"Resuscitation-promoting factor RpfB","featureId":"PRO_0000421027"},{"type":"Domain","location":{"start":{"value":192,"modifier":"EXACT"},"end":{"value":272,"modifier":"EXACT"}},"description":"G5","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00437"}]},{"type":"Lipidation","location":{"start":{"value":24,"modifier":"EXACT"},"end":{"value":24,"modifier":"EXACT"}},"description":"N-palmitoyl cysteine","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00303"}]},{"type":"Lipidation","location":{"start":{"value":24,"modifier":"EXACT"},"end":{"value":24,"modifier":"EXACT"}},"description":"S-diacylglycerol cysteine","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00303"}]},{"type":"Disulfide bond","location":{"start":{"value":291,"modifier":"EXACT"},"end":{"value":355,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000250"}]}],"keywords":[{"id":"KW-1003","category":"Cellular component","name":"Cell membrane"},{"id":"KW-1015","category":"PTM","name":"Disulfide bond"},{"id":"KW-0378","category":"Molecular function","name":"Hydrolase"},{"id":"KW-0449","category":"PTM","name":"Lipoprotein"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-0564","category":"PTM","name":"Palmitate"},{"id":"KW-0732","category":"Domain","name":"Signal"},{"id":"KW-0843","category":"Biological process","name":"Virulence"}],"references":[{"referenceNumber":1,"citation":{"id":"22535945","citationType":"journal article","authors":["Miyoshi-Akiyama T.","Matsumura K.","Iwai H.","Funatogawa K.","Kirikae T."],"citationCrossReferences":[{"database":"PubMed","id":"22535945"},{"database":"DOI","id":"10.1128/jb.00353-12"}],"title":"Complete annotated genome sequence of Mycobacterium tuberculosis Erdman.","publicationDate":"2012","journal":"J. Bacteriol.","firstPage":"2770","lastPage":"2770","volume":"194"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"ATCC 35801 / TMC 107 / Erdman","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"16622237","citationType":"journal article","authors":["Tufariello J.M.","Mi K.","Xu J.","Manabe Y.C.","Kesavan A.K.","Drumm J.","Tanaka K.","Jacobs W.R. Jr.","Chan J."],"citationCrossReferences":[{"database":"PubMed","id":"16622237"},{"database":"DOI","id":"10.1128/iai.74.5.2985-2995.2006"}],"title":"Deletion of the Mycobacterium tuberculosis resuscitation-promoting factor Rv1009 gene results in delayed reactivation from chronic tuberculosis.","publicationDate":"2006","journal":"Infect. Immun.","firstPage":"2985","lastPage":"2995","volume":"74"},"referencePositions":["INDUCTION","DISRUPTION PHENOTYPE"],"referenceComments":[{"value":"ATCC 35801 / TMC 107 / Erdman","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AP012340","properties":[{"key":"ProteinId","value":"BAL64927.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"H8EZH5","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"H8EZH5","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"mtn:ERDMAN_1122","properties":[{"key":"Description","value":"-"}]},{"database":"PATRIC","id":"fig|652616.3.peg.1135","properties":[{"key":"GeneDesignation","value":"-"}]},{"database":"HOGENOM","id":"CLU_036884_1_0_11","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005576","properties":[{"key":"GoTerm","value":"C:extracellular region"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-ARBA"}]},{"database":"GO","id":"GO:0005886","properties":[{"key":"GoTerm","value":"C:plasma membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0016787","properties":[{"key":"GoTerm","value":"F:hydrolase activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0010629","properties":[{"key":"GoTerm","value":"P:negative regulation of gene expression"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-ARBA"}]},{"database":"GO","id":"GO:0009372","properties":[{"key":"GoTerm","value":"P:quorum sensing"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-ARBA"}]},{"database":"GO","id":"GO:0042127","properties":[{"key":"GoTerm","value":"P:regulation of cell population proliferation"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-ARBA"}]},{"database":"CDD","id":"cd13925","properties":[{"key":"EntryName","value":"RPF"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.10.530.10:FF:000015","properties":[{"key":"EntryName","value":"Resuscitation-promoting factor RpfB"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"2.20.230.10:FF:000003","properties":[{"key":"EntryName","value":"Resuscitation-promoting factor RpfB"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.530.10","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.20.230.10","properties":[{"key":"EntryName","value":"Resuscitation-promoting factor rpfb"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR007137","properties":[{"key":"EntryName","value":"DUF348"}]},{"database":"InterPro","id":"IPR011098","properties":[{"key":"EntryName","value":"G5_dom"}]},{"database":"InterPro","id":"IPR023346","properties":[{"key":"EntryName","value":"Lysozyme-like_dom_sf"}]},{"database":"InterPro","id":"IPR051933","properties":[{"key":"EntryName","value":"Resuscitation_pf_RpfB"}]},{"database":"InterPro","id":"IPR010618","properties":[{"key":"EntryName","value":"RPF"}]},{"database":"PANTHER","id":"PTHR39160","properties":[{"key":"EntryName","value":"CELL WALL-BINDING PROTEIN YOCH"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR39160:SF4","properties":[{"key":"EntryName","value":"RESUSCITATION-PROMOTING FACTOR RPFB"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF03990","properties":[{"key":"EntryName","value":"DUF348"},{"key":"MatchStatus","value":"3"}]},{"database":"Pfam","id":"PF07501","properties":[{"key":"EntryName","value":"G5"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF06737","properties":[{"key":"EntryName","value":"Transglycosylas"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM01208","properties":[{"key":"EntryName","value":"G5"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF53955","properties":[{"key":"EntryName","value":"Lysozyme-like"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51109","properties":[{"key":"EntryName","value":"G5"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51257","properties":[{"key":"EntryName","value":"PROKAR_LIPOPROTEIN"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MLRLVVGALLLVLAFAGGYAVAACKTVTLTVDGTAMRVTTMKSRVIDIVEENGFSVDDRDDLYPAAGVQVHDADTIVLRRSRPLQISLDGHDAKQVWTTASTVDEALAQLAMTDTAPAAASRASRVPLSGMALPVVSAKTVQLNDGGLVRTVHLPAPNVAGLLSAAGVPLLQSDHVVPAATAPIVEGMQIQVTRNRIKKVTERLPLPPNARRVEDPEMNMSREVVEDPGVPGTQDVTFAVAEVNGVETGRLPVANVVVTPAHEAVVRVGTKPGTEVPPVIDGSIWDAIAGCEAGGNWAINTGNGYYGGVQFDQGTWEANGGLRYAPRADLATREEQIAVAEVTRLRQGWGAWPVCAARAGAR","length":362,"molWeight":38078,"crc64":"02B55D8C70373D10","md5":"196E9E4888DF8ADF7D328A03B33805C5"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBCELLULAR LOCATION":1,"INDUCTION":1,"DISRUPTION PHENOTYPE":1,"SIMILARITY":1},"countByFeatureType":{"Signal":1,"Chain":1,"Domain":1,"Lipidation":2,"Disulfide bond":1},"uniParcId":"UPI0000030103"}}