{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"H0QPM1","uniProtkbId":"CGDB1_ARTG1","entryAudit":{"firstPublicDate":"2024-10-02","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2012-02-22","entryVersion":26,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Arthrobacter globiformis (strain ATCC 8010 / DSM 20124 / JCM 1332 / NBRC 12137 / NCIMB 8907 / NRRL B-2979 / 168)","taxonId":1077972,"lineage":["Bacteria","Bacillati","Actinomycetota","Actinomycetes","Micrococcales","Micrococcaceae","Arthrobacter"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"34728636"}],"value":"C-glycoside deglycosidase beta subunit"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"34728636"}],"value":"CGD beta subunit"}],"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34728636"}],"value":"4.1.99.-"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"34728636"}],"value":"AgCGD1 beta"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"34728636"}],"value":"C-deglycosylation enzyme beta subunit"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"34728636"}],"value":"carC1"},"orfNames":[{"evidences":[{"evidenceCode":"ECO:0000312","source":"EMBL","id":"GAB14772.1"}],"value":"ARGLB_075_00550"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34728636"}],"value":"Carbon-carbon bond-cleaving enzyme which participates in the metabolism of C-glycosides (PubMed:34728636). Acts on the C6-glycosylated compound 3''-dehydroisovitexin (3''-oxo-isovitexin) (PubMed:34728636). Shows weak activity with 3''-dehydroisoorientin (3''-oxo-homoorientin) and 3'-dehydromangiferin (3'-oxo-mangiferin) (PubMed:34728636)"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"3''-dehydroisovitexin = 1,5-anhydro-D-erythro-hex-1-en-3-ulose + apigenin","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:78775"},{"database":"ChEBI","id":"CHEBI:58470"},{"database":"ChEBI","id":"CHEBI:194219"},{"database":"ChEBI","id":"CHEBI:195275"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34728636"}]}},{"commentType":"COFACTOR","cofactors":[{"name":"Mg(2+)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34728636"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:18420"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34728636"}],"value":"Activity is strongly reduced in the presence of chelating agents"}],"commentType":"ACTIVITY REGULATION"},{"commentType":"BIOPHYSICOCHEMICAL PROPERTIES","kineticParameters":{"michaelisConstants":[{"constant":3.5,"unit":"mM","substrate":"3''-dehydroisovitexin","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34728636"}]},{"constant":3.4,"unit":"mM","substrate":"3''-dehydroisoorientin","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34728636"}]},{"constant":3.7,"unit":"mM","substrate":"3'-dehydromangiferin","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34728636"}]}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34728636"}],"value":"kcat is 4.6 min(-1) with 3''-dehydroisovitexin as substrate. kcat is 0.57 min(-1) with 3''-dehydroisoorientin as substrate. kcat is 0.17 min(-1) with 3'-dehydromangiferin as substrate."}]}},"phDependence":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34728636"}],"value":"Optimum pH is 7.5."}]},"temperatureDependence":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34728636"}],"value":"Optimum temperature is around 40 degrees Celsius."}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34728636"}],"value":"Heterodimer composed of an alpha subunit (CarB1) and a beta subunit (CarC1)"}],"commentType":"SUBUNIT"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the C-glycoside deglycosidase beta subunit family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":131,"modifier":"EXACT"}},"description":"C-glycoside deglycosidase beta subunit","featureId":"PRO_0000461028"}],"keywords":[{"id":"KW-0119","category":"Biological process","name":"Carbohydrate metabolism"},{"id":"KW-0456","category":"Molecular function","name":"Lyase"},{"id":"KW-0460","category":"Ligand","name":"Magnesium"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-999UPBT730V81","citationType":"submission","authors":["Miyazawa S.","Hosoyama A.","Tsuchikane K.","Katsumata H.","Yamazaki S.","Fujita N."],"title":"Whole genome shotgun sequence of Arthrobacter globiformis NBRC 12137.","publicationDate":"DEC-2011","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"ATCC 8010 / DSM 20124 / JCM 1332 / NBRC 12137 / NCIMB 8907 / NRRL B-2979 / 168","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"34728636","citationType":"journal article","authors":["Mori T.","Kumano T.","He H.","Watanabe S.","Senda M.","Moriya T.","Adachi N.","Hori S.","Terashita Y.","Kawasaki M.","Hashimoto Y.","Awakawa T.","Senda T.","Abe I.","Kobayashi M."],"citationCrossReferences":[{"database":"PubMed","id":"34728636"},{"database":"DOI","id":"10.1038/s41467-021-26585-1"}],"title":"C-Glycoside metabolism in the gut and in nature: Identification, characterization, structural analyses and distribution of C-C bond-cleaving enzymes.","publicationDate":"2021","journal":"Nat. Commun.","firstPage":"6294","lastPage":"6294","volume":"12"},"referencePositions":["FUNCTION","CATALYTIC ACTIVITY","COFACTOR","ACTIVITY REGULATION","BIOPHYSICOCHEMICAL PROPERTIES","SUBUNIT"],"referenceComments":[{"value":"ATCC 8010 / DSM 20124 / JCM 1332 / NBRC 12137 / NCIMB 8907 / NRRL B-2979 / 168","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"BAEG01000075","properties":[{"key":"ProteinId","value":"GAB14772.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_003803560.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_BAEG01000075.1"}]},{"database":"AlphaFoldDB","id":"H0QPM1","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"H0QPM1","properties":[{"key":"Description","value":"-"}]},{"database":"STRING","id":"1077972.ARGLB_075_00550","properties":[{"key":"Description","value":"-"}]},{"database":"OrthoDB","id":"1494151at2","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000003828","properties":[{"key":"Component","value":"Unassembled WGS sequence"}]},{"database":"InterPro","id":"IPR045959","properties":[{"key":"EntryName","value":"CGDB"}]},{"database":"Pfam","id":"PF19906","properties":[{"key":"EntryName","value":"CGDB"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MIADRIIEHGTLITRDGRAAVEVRIPWYRALPGSCIAGAALTVDGVAAPEDTLRWTMNNRTFSFEELVDETGEWWFPLDSAVLSGDLPVWDDQAEHEVRVDLKLCIPYIITDHGVLHIEEHDTKTMKVAQQ","length":131,"molWeight":14769,"crc64":"1A77CDCC7B2BE842","md5":"657E344EBA5977E3457F0D45A0EF5CA4"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"COFACTOR":1,"ACTIVITY REGULATION":1,"BIOPHYSICOCHEMICAL PROPERTIES":1,"SUBUNIT":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1},"uniParcId":"UPI00023A8A64"}}