{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"G8XHD8","uniProtkbId":"BESA_STREN","entryAudit":{"firstPublicDate":"2019-06-05","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2012-02-22","entryVersion":52,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Streptantibioticus cattleyicolor (strain ATCC 35852 / DSM 46488 / JCM 4925 / NBRC 14057 / NRRL 8057)","commonName":"Streptomyces cattleya","taxonId":1003195,"lineage":["Bacteria","Bacillati","Actinomycetota","Actinomycetes","Kitasatosporales","Streptomycetaceae","Streptantibioticus"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"30867596"}],"value":"L-propargylglycine--L-glutamate ligase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30867596"}],"value":"6.3.2.-"}]}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"30867596"}],"value":"besA"},"orderedLocusNames":[{"evidences":[{"evidenceCode":"ECO:0000312","source":"EMBL","id":"AEW98884.1"}],"value":"SCATT_p06910"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30867596"}],"value":"Involved in the biosynthesis of terminal alkyne-containing amino acids such as L-beta-ethynylserine, that are produced as antibiotics by S.cattleya. Catalyzes the ATP-dependent ligation of L-propargylglycine to L-glutamate to form the dipeptide L-gamma-glutamyl-L-propargylglycine. Is selective for L-propargylglycine over norvaline, allylglycine and the standard proteinogenic amino acids, except L-cysteine which can be used as a substrate to a lesser extent"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"L-propargylglycine + L-glutamate + ATP = L-gamma-glutamyl-L-propargylglycine + ADP + phosphate + 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terminal alkyne-containing amino acids produced by wild-type S.cattleya"}],"commentType":"DISRUPTION PHENOTYPE"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":482,"modifier":"EXACT"}},"description":"L-propargylglycine--L-glutamate ligase","featureId":"PRO_0000447348"}],"geneLocations":[{"value":"pSCATT","geneEncodingType":"Plasmid"}],"keywords":[{"id":"KW-0002","category":"Technical term","name":"3D-structure"},{"id":"KW-0028","category":"Biological process","name":"Amino-acid biosynthesis"},{"id":"KW-0045","category":"Biological process","name":"Antibiotic biosynthesis"},{"id":"KW-0067","category":"Ligand","name":"ATP-binding"},{"id":"KW-0436","category":"Molecular function","name":"Ligase"},{"id":"KW-0547","category":"Ligand","name":"Nucleotide-binding"},{"id":"KW-0614","category":"Technical term","name":"Plasmid"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-22QLSAVDVHUML","citationType":"submission","authors":["Ou H.-Y.","Li P.","Zhao C.","O'Hagan D.","Deng Z."],"title":"Complete genome sequence of Streptomyces cattleya strain DSM 46488.","publicationDate":"DEC-2011","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"ATCC 35852 / DSM 46488 / JCM 4925 / NBRC 14057 / NCIMB 11928 / NRRL 8057 / MA-4297","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"30867596","citationType":"journal article","authors":["Marchand J.A.","Neugebauer M.E.","Ing M.C.","Lin C.I.","Pelton J.G.","Chang M.C.Y."],"citationCrossReferences":[{"database":"PubMed","id":"30867596"},{"database":"DOI","id":"10.1038/s41586-019-1020-y"}],"title":"Discovery of a pathway for terminal-alkyne amino acid 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