{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"G4V4G3","secondaryAccessions":["V3V7V7"],"uniProtkbId":"YGFX_PROCF","entryAudit":{"firstPublicDate":"2017-02-15","lastAnnotationUpdateDate":"2026-06-10","lastSequenceUpdateDate":"2011-12-14","entryVersion":35,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Prodigiosinella confusarubida","commonName":"Serratia sp. (strain ATCC 39006)","taxonId":104623,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Enterobacterales","Pectobacteriaceae","Prodigiosinella"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Inner membrane protein YgfX"}}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"22474332"}],"value":"ygfX"},"synonyms":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"23657679"}],"value":"cptA"}],"orfNames":[{"value":"Ser39006_01162"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22474332"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23657679"}],"value":"Required for production of prodigiosin antibiotic (Pig); overproduction in ygfX, sdhE or double sdhE-ygfX deletion mutants leads to nearly 10-fold increased synthesis of Pig (PubMed:22474332, PubMed:23657679). Overexpressing cells have somewhat altered cell length depending on growth phase (PubMed:23657679). Does not function as a toxin; overexpression in the presence or absence of sdhE (the putative antitoxin) has no effect on growth rate (PubMed:23657679)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22474332"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23657679"}],"value":"Antibiotic biosynthesis; prodigiosin biosynthesis"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23657679"}],"value":"Interacts with itself, SdhE and as in E.coli FtsZ and MreB; other interactions are shown but require validation (PubMed:23657679)"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"22474332"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"23657679"}],"value":"Cell inner membrane","id":"SL-0037"},"topology":{"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"23657679"}],"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22474332"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24374335"}],"value":"Transcribed under both aerobic (with succinate or glucose) and anaerobic (with glycerol with or without fumarate) conditions (PubMed:24374335). Part of the sdhE-ygfX operon (PubMed:22474332)"}],"commentType":"INDUCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23657679"}],"value":"The intact transmembrane domain is required for Pig production, its replacement with 2 TMs from an E.coli membrane protein (MalF) does not allow Pig synthesis; although the chimeric protein is found in the membrane it does not self-associate"}],"commentType":"DOMAIN"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22474332"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23657679"}],"value":"No effect on aerobic growth on succinate or in LB. 90% decreased synthesis of prodigiosin antibiotic, decreased transcription of flavoprotein desaturase PigA (PubMed:22474332, PubMed:23657679)"}],"commentType":"DISRUPTION PHENOTYPE"},{"commentType":"SEQUENCE CAUTION","sequenceCautionType":"Erroneous initiation","sequence":"ESN63754.1","note":"Truncated N-terminus.","evidences":[{"evidenceCode":"ECO:0000305"}]}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":143,"modifier":"EXACT"}},"description":"Inner membrane protein YgfX","featureId":"PRO_0000438888"},{"type":"Topological domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":11,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"23657679"}]},{"type":"Transmembrane","location":{"start":{"value":12,"modifier":"EXACT"},"end":{"value":31,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"23657679"}]},{"type":"Topological domain","location":{"start":{"value":32,"modifier":"EXACT"},"end":{"value":35,"modifier":"EXACT"}},"description":"Periplasmic","evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"23657679"}]},{"type":"Transmembrane","location":{"start":{"value":36,"modifier":"EXACT"},"end":{"value":54,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"23657679"}]},{"type":"Topological domain","location":{"start":{"value":55,"modifier":"EXACT"},"end":{"value":143,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"23657679"}]},{"type":"Mutagenesis","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":54,"modifier":"EXACT"}},"description":"Protein only partially membrane-associated, no Pig production, 100% SDH activity observed.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23657679"}],"alternativeSequence":{}},{"type":"Mutagenesis","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":13,"modifier":"EXACT"}},"description":"Less Pig production than wild-type, protein not detectable when His-tagged.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23657679"}],"alternativeSequence":{}},{"type":"Mutagenesis","location":{"start":{"value":34,"modifier":"EXACT"},"end":{"value":34,"modifier":"EXACT"}},"description":"Very low Pig production, poor self-interaction, no longer interacts with SdhE, protein accumulates stably in membrane.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23657679"}],"alternativeSequence":{"originalSequence":"W","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":117,"modifier":"EXACT"},"end":{"value":117,"modifier":"EXACT"}},"description":"Very low Pig production, no self-interaction, no longer interacts with SdhE, protein accumulates stably in membrane.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23657679"}],"alternativeSequence":{"originalSequence":"D","alternativeSequences":["A"]}}],"keywords":[{"id":"KW-0045","category":"Biological process","name":"Antibiotic biosynthesis"},{"id":"KW-0997","category":"Cellular component","name":"Cell inner membrane"},{"id":"KW-1003","category":"Cellular component","name":"Cell membrane"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"id":"KW-1133","category":"Domain","name":"Transmembrane helix"}],"references":[{"referenceNumber":1,"citation":{"id":"22474332","citationType":"journal article","authors":["McNeil M.B.","Clulow J.S.","Wilf N.M.","Salmond G.P.","Fineran P.C."],"citationCrossReferences":[{"database":"PubMed","id":"22474332"},{"database":"DOI","id":"10.1074/jbc.m111.293803"}],"title":"SdhE is a conserved protein required for flavinylation of succinate dehydrogenase in bacteria.","publicationDate":"2012","journal":"J. 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