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CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Functions as a ssRNA-specific endoribonuclease. Involved in the integration of spacer DNA into the CRISPR cassette (By similarity)"}],"commentType":"FUNCTION"},{"commentType":"COFACTOR","cofactors":[{"name":"Mg(2+)","evidences":[{"evidenceCode":"ECO:0000250"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:18420"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22408157"}],"value":"Homodimer, forms a heterotetramer with a Cas1 homodimer (By similarity). Can form a Cascis complex with Cas4 and Csa1"}],"commentType":"SUBUNIT"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22408157"}],"value":"Slightly induced by 20 J/m2 ultraviolet light. Member of the csa1-cas1/2-cas4 operon"}],"commentType":"INDUCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"In the N-terminal section; belongs to the CRISPR-associated endonuclease Cas1 family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"In the C-terminal section; belongs to the CRISPR-associated endoribonuclease Cas2 protein family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Neither Cas1 nor Cas2 are fully present in this fusion protein, however as this is the only copy of these proteins in this organism they are probably functional. The Cas1 section is much smaller than usual and it is quite atypical"}],"commentType":"CAUTION"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":264,"modifier":"EXACT"}},"description":"CRISPR-associated endonuclease Cas1/endonuclease Cas2 fusion","featureId":"PRO_0000422226"},{"type":"Region","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":104,"modifier":"EXACT"}},"description":"CRISPR-associated endonuclease Cas1"},{"type":"Region","location":{"start":{"value":108,"modifier":"EXACT"},"end":{"value":167,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":176,"modifier":"EXACT"},"end":{"value":264,"modifier":"EXACT"}},"description":"CRISPR-associated endonuclease Cas2"},{"type":"Compositional bias","location":{"start":{"value":143,"modifier":"EXACT"},"end":{"value":155,"modifier":"EXACT"}},"description":"Low complexity","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Binding site","location":{"start":{"value":182,"modifier":"EXACT"},"end":{"value":182,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000255"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","note":"catalytic"}}],"keywords":[{"id":"KW-0051","category":"Biological process","name":"Antiviral defense"},{"id":"KW-0255","category":"Molecular function","name":"Endonuclease"},{"id":"KW-0378","category":"Molecular function","name":"Hydrolase"},{"id":"KW-0460","category":"Ligand","name":"Magnesium"},{"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"id":"KW-0540","category":"Molecular function","name":"Nuclease"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"}],"references":[{"referenceNumber":1,"citation":{"id":"22003381","citationType":"journal article","authors":["Siebers B.","Zaparty M.","Raddatz G.","Tjaden B.","Albers S.V.","Bell S.D.","Blombach F.","Kletzin A.","Kyrpides N.","Lanz C.","Plagens A.","Rampp M.","Rosinus A.","von Jan M.","Makarova K.S.","Klenk H.P.","Schuster S.C.","Hensel R."],"citationCrossReferences":[{"database":"PubMed","id":"22003381"},{"database":"DOI","id":"10.1371/journal.pone.0024222"}],"title":"The complete genome sequence of Thermoproteus tenax: a physiologically versatile member of the Crenarchaeota.","publicationDate":"2011","journal":"PLoS ONE","firstPage":"E24222","lastPage":"E24222","volume":"6"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"ATCC 35583 / DSM 2078 / JCM 9277 / NBRC 100435 / Kra 1","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"22408157","citationType":"journal article","authors":["Plagens A.","Tjaden B.","Hagemann A.","Randau L.","Hensel R."],"citationCrossReferences":[{"database":"PubMed","id":"22408157"},{"database":"DOI","id":"10.1128/jb.00206-12"}],"title":"Characterization of the CRISPR/Cas subtype I-A system of the hyperthermophilic crenarchaeon Thermoproteus tenax.","publicationDate":"2012","journal":"J. Bacteriol.","firstPage":"2491","lastPage":"2500","volume":"194"},"referencePositions":["SUBUNIT","INDUCTION","OPERON STRUCTURE"],"referenceComments":[{"value":"ATCC 35583 / DSM 2078 / JCM 9277 / NBRC 100435 / Kra 1","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"FN869859","properties":[{"key":"ProteinId","value":"CCC81881.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_014127136.1","properties":[{"key":"NucleotideSequenceId","value":"NC_016070.1"}]},{"database":"AlphaFoldDB","id":"G4RJY6","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"G4RJY6","properties":[{"key":"Description","value":"-"}]},{"database":"STRING","id":"768679.TTX_1246","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"768679-TTX_1246","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"11262126","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ttn:TTX_1246","properties":[{"key":"Description","value":"-"}]},{"database":"PATRIC","id":"fig|768679.9.peg.1259","properties":[{"key":"GeneDesignation","value":"-"}]},{"database":"eggNOG","id":"arCOG01452","properties":[{"key":"ToxonomicScope","value":"Archaea"}]},{"database":"eggNOG","id":"arCOG04194","properties":[{"key":"ToxonomicScope","value":"Archaea"}]},{"database":"HOGENOM","id":"CLU_1052197_0_0_2","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000002654","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"GO","id":"GO:0046872","properties":[{"key":"GoTerm","value":"F:metal ion binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0003676","properties":[{"key":"GoTerm","value":"F:nucleic acid binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0004521","properties":[{"key":"GoTerm","value":"F:RNA endonuclease activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0051607","properties":[{"key":"GoTerm","value":"P:defense response to virus"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0043571","properties":[{"key":"GoTerm","value":"P:maintenance of CRISPR repeat elements"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"Gene3D","id":"3.30.70.240","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_01471","properties":[{"key":"EntryName","value":"Cas2"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR002729","properties":[{"key":"EntryName","value":"CRISPR-assoc_Cas1"}]},{"database":"InterPro","id":"IPR021127","properties":[{"key":"EntryName","value":"CRISPR_associated_Cas2"}]},{"database":"InterPro","id":"IPR019199","properties":[{"key":"EntryName","value":"Virulence_VapD/CRISPR_Cas2"}]},{"database":"NCBIfam","id":"TIGR01573","properties":[{"key":"EntryName","value":"cas2"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR34405","properties":[{"key":"EntryName","value":"CRISPR-ASSOCIATED ENDORIBONUCLEASE CAS2"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR34405:SF3","properties":[{"key":"EntryName","value":"CRISPR-ASSOCIATED ENDORIBONUCLEASE CAS2 3"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01867","properties":[{"key":"EntryName","value":"Cas_Cas1"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF09827","properties":[{"key":"EntryName","value":"CRISPR_Cas2"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF143430","properties":[{"key":"EntryName","value":"TTP0101/SSO1404-like"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MDEVLLLTGGISITTRALRALLATGATVAVFSPRGEPLGIFMRPVGDATGAKRRCQYKAAEDGRGLQYAKSWVFKKILGQRDNIKAWRRRLRGYSQYAESLAKALPGAGLHGAMETPRRRRRGQDGGQAGVRGRPTHPPVPPGAGRRSPGGAPRGQEASLRRDPQRGQSSGALHMYVIVVYDITENDVRAKVADILRAYGLARIQRSAYVGRLPPALVKELAERLARAVRGANADIAIFKVDKRTIDTSLRIPPRPPAGHVALH","length":264,"molWeight":28671,"crc64":"DE5DB4F0C6367AF9","md5":"923322A8A3CE0479058F4039F6DF606A"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"COFACTOR":1,"SUBUNIT":1,"INDUCTION":1,"SIMILARITY":2,"CAUTION":1},"countByFeatureType":{"Chain":1,"Region":3,"Compositional bias":1,"Binding site":1},"uniParcId":"UPI00022BCB36"}}