{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"F5HHY1","uniProtkbId":"CEP3_HHV8P","entryAudit":{"firstPublicDate":"2013-10-16","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2011-06-28","entryVersion":36,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Human herpesvirus 8 type P (isolate GK18)","commonName":"HHV-8","synonyms":["Kaposi's sarcoma-associated herpesvirus"],"taxonId":868565,"lineage":["Viruses","Duplodnaviria","Heunggongvirae","Peploviricota","Herviviricetes","Herpesvirales","Orthoherpesviridae","Gammaherpesvirinae","Rhadinovirus","Rhadinovirus humangamma8","Human herpesvirus 8"]},"organismHosts":[{"scientificName":"Homo sapiens","commonName":"Human","taxonId":9606}],"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_04042"}],"value":"Cytoplasmic envelopment protein 3"}}},"genes":[{"geneName":{"value":"ORF38"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_04042"}],"value":"Plays an important role in the cytoplasmic envelopment of tegument proteins and capsids during the assembly and egress processes. Also participates in viral entry at the fusion step probably by regulating the core fusion machinery"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_04042"}],"value":"Interacts with cytoplasmic envelopment protein 2; this interaction is essential for the proper localization of each protein to the assembly complex and thus for the production of infectious virus"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_04042"}],"value":"Virion membrane-associated tegument protein. Associates with host membrane lipids rafts. During virion morphogenesis, this protein probably accumulates in the endosomes and trans-Golgi where secondary envelopment occurs. It is probably transported to the cell surface from where it is endocytosed and directed to the trans-Golgi network (TGN)"}]},"subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_04042"}],"value":"Virion tegument","id":"SL-0273"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_04042"}],"value":"Virion membrane","id":"SL-0275"},"topology":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_04042"}],"value":"Lipid-anchor","id":"SL-9901"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_04042"}],"value":"Host cell membrane","id":"SL-0375"},"topology":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_04042"}],"value":"Lipid-anchor","id":"SL-9901"},"orientation":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_04042"}],"value":"Cytoplasmic side","id":"SL-9910"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_04042"}],"value":"Host Golgi apparatus membrane","id":"SL-0426"},"topology":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_04042"}],"value":"Lipid-anchor","id":"SL-9901"},"orientation":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_04042"}],"value":"Cytoplasmic side","id":"SL-9910"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_04042"}],"value":"Myristoylation and palmitoylation (probably on one or more of the nearby cysteines at the N-terminus) enable membrane-binding and Golgi apparatus-specific targeting and are essential for efficient packaging"}],"commentType":"PTM"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_04042"}],"value":"Phosphorylated. Phosphorylation does not seem to be required for recycling to the host Golgi apparatus. Packaging is selective for underphosphorylated forms"}],"commentType":"PTM"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_04042"}],"value":"Belongs to the herpesviridae cytoplasmic envelopment protein 3 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Initiator methionine","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"Removed; by host","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_04042"}]},{"type":"Chain","location":{"start":{"value":2,"modifier":"EXACT"},"end":{"value":61,"modifier":"EXACT"}},"description":"Cytoplasmic envelopment protein 3","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_04042"}],"featureId":"PRO_0000423784"},{"type":"Lipidation","location":{"start":{"value":2,"modifier":"EXACT"},"end":{"value":2,"modifier":"EXACT"}},"description":"N-myristoyl glycine; by host","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_04042"}]}],"keywords":[{"id":"KW-1032","category":"Cellular component","name":"Host cell membrane"},{"id":"KW-1040","category":"Cellular component","name":"Host Golgi apparatus"},{"id":"KW-1043","category":"Cellular component","name":"Host membrane"},{"id":"KW-0449","category":"PTM","name":"Lipoprotein"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-0519","category":"PTM","name":"Myristate"},{"id":"KW-0564","category":"PTM","name":"Palmitate"},{"id":"KW-0597","category":"PTM","name":"Phosphoprotein"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0946","category":"Cellular component","name":"Virion"},{"id":"KW-0920","category":"Cellular component","name":"Virion tegument"}],"references":[{"referenceNumber":1,"citation":{"id":"10400794","citationType":"journal article","authors":["Glenn M.","Rainbow L.","Aurade F.","Davison A.","Schulz T.F."],"citationCrossReferences":[{"database":"PubMed","id":"10400794"},{"database":"DOI","id":"10.1128/jvi.73.8.6953-6963.1999"}],"title":"Identification of a spliced gene from Kaposi's sarcoma-associated herpesvirus encoding a protein with similarities to latent membrane proteins 1 and 2A of Epstein-Barr virus.","publicationDate":"1999","journal":"J. 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